LOCUS       pOCC254-MBP-Pab1        8592 bp    DNA     circular UNA 08-APR-2024
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local...4i-hkp6tf1
VERSION     urn.local...4i-hkp6tf1
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..2179
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pOCC254-eIF4G (digested by NotI, AscI)
                     Fragment 2 - Digestion of pOCC254_pOEM1_N_ - Fragment 2
                     (concatenated sequence 1) (concatenated sequence 1)"
     promoter        57..151
     misc_feature    81..169
                     /standard_name="PH_promoter"
     misc_feature    174..201
                     /standard_name="POEM1-F primer"
     misc_feature    230..247
                     /standard_name="6xHis"
     exon            260..1402
                     /standard_name="MBP from pMAL-c5G(1)"
     exon            260..1365
                     /standard_name="MBP from pMAL-c5G"
     primer_bind     1351..1368
                     /standard_name=1995
     misc_feature    1415..1438
                     /standard_name="PreScission"
     gene            1439..2146
                     /standard_name="Cherry in pOCC"
     primer_bind     2047..2064
                     /standard_name="pOCC254-fw"
     misc_feature    2147..2152
                     /standard_name="linker"
     misc_feature    2153..2173
                     /standard_name="TEV-site"
     misc_feature    2153..2173
                     /standard_name="TEV cleavage site"
     misc_feature    2176..3919
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pOCC102-MBP-Pab1 (digested by NotI, AscI)
                     Fragment 1 (concatenated sequence 2)"
     misc_feature    2176..3919
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="PAB1 (digested by AscI, NotI) Fragment 2
                     (concatenated sequence 2)"
     misc_feature    <2176..2179
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="NotI/AscI Digestion of pOCC102
                     (concatenated sequence 1)"
     misc_feature    <2176..>2181
                     /note="Geneious type: ORF"
                     /standard_name="eIF4G"
     misc_feature    2176..2179
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    <2176..2182
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    join(3916..>3919,<2176..2179)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="NotI/AscI Digestion of pOCC102
                     (concatenated sequence 1)"
     misc_feature    <2176..>2179
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    join(2176..>2179,<3916..3919)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of NotI/AscI Tif4631 optimized
                     (IDT) no STOP - Fragment 2 (concatenated sequence 2)"
     misc_feature    3914..>3919
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TAA"
     misc_feature    <3914..>3919
                     /note="Geneious type: ORF"
                     /standard_name="eIF4G"
     misc_feature    3916..>3919
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="NotI/AscI Digestion of pOCC102
                     (concatenated sequence 1)"
     misc_feature    3916..3919
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    3916..8592
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pOCC254-eIF4G (digested by NotI, AscI)
                     Fragment 2 - Digestion of pOCC254_pOEM1_N_ - Fragment 2
                     (concatenated sequence 1) (concatenated sequence 3)"
     misc_feature    <3916..>3919
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TAA"
     misc_feature    3916..2179
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pOCC254_pOEM1_N_ - Fragment 2
                     (concatenated sequence 1)"
     misc_feature    3963..3993
                     /standard_name="POEM1-R primer"
     polyA_signal    complement(3997..4188)
     primer_bind     complement(4057..4074)
                     /standard_name="pOCC254-rev"
     polyA_site      4089..4123
     rep_origin      complement(5081..5763)
     CDS             complement(5858..6718)
                     /standard_name="AmpR"
     misc_feature    complement(5861..5879)
                     /standard_name="AP0049.Amp-CF"
     misc_feature    complement(6699..6718)
                     /standard_name="AP0052.Amp-nF"
     misc_feature    6699..6718
                     /standard_name="AP0053.Amp-nR"
     promoter        complement(6754..6789)
     primer_bind     7139..7156
                     /standard_name="M13-fwd"
     primer_bind     7166..7186
                     /standard_name="T7"
ORIGIN      
        1 agctgccacc attttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtat
       61 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
      121 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
      181 attcataccg tcccaccatc gggcgcggat cgccaccatg ggcagcagcc atcaccatca
      241 ccatcacagc agcggcagaa tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg
      301 cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat
      361 taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac
      421 tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc
      481 tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac
      541 ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt
      601 atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc
      661 ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga
      721 accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa
      781 cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac
      841 cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc
      901 agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc
      961 caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca
     1021 accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa
     1081 agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt
     1141 taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggtgaa
     1201 agatccgcgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat
     1261 cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg
     1321 tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa
     1381 caacaataac aataacaaca acagcagcgg cagactggaa gttctgttcc aggggccgat
     1441 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     1501 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     1561 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     1621 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     1681 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     1741 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     1801 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     1861 gcagaagaag acgatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     1921 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     1981 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     2041 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     2101 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggat ccgaaaactt
     2161 gtatttccag ggcgcggccg caatggctga tattactgat aagacagctg aacaattgga
     2221 aaacttgaat attcaagatg accaaaagca agccgccact ggttcagaaa gccaatctgt
     2281 tgaaaactct tctgcatcat tatatgttgg tgacttagaa ccttctgttt ccgaagccca
     2341 cttatatgat atcttctctc caatcggttc agtctcctcc attcgtgtct gtcgtgatgc
     2401 catcactaag acctctttgg gctatgctta tgttaacttt aacgaccatg aagccggcag
     2461 aaaagcaatt gagcaattga actacactcc aatcaagggt agattatgcc gtattatgtg
     2521 gtctcaacgt gacccatcat tgagaaagaa gggttctggt aacatcttta tcaagaactt
     2581 gcaccctgat attgacaaca aggctttgta tgacactttc tctgtgtttg gtgacatctt
     2641 gtccagcaag attgccaccg acgaaaacgg aaaatccaag ggttttgggt ttgttcactt
     2701 cgaagaagaa ggtgctgcca aggaagctat tgatgctttg aatggtatgc tgttgaacgg
     2761 tcaagaaatt tatgttgctc ctcacttgtc cagaaaggaa cgtgactctc aattggaaga
     2821 gactaaggca cattacacta acctttatgt gaaaaacatc aactccgaaa ctactgacga
     2881 acaattccaa gaattgtttg ccaaatttgg tccaattgtt tctgcctctt tggaaaagga
     2941 tgctgatgga aaattgaagg gtttcgggtt tgttaactac gaaaagcatg aagacgctgt
     3001 gaaagctgtt gaagctttga atgactctga actaaatgga gaaaagttat acgttggtcg
     3061 tgcccaaaag aagaatgaac gtatgcatgt cttgaagaag caatacgaag cttacagatt
     3121 ggaaaaaatg gccaagtacc aaggtgttaa tttgtttgtt aagaacttag atgacagcgt
     3181 tgatgacgaa aagttggaag aagaatttgc tccatatggt actatcactt ctgcaaaggt
     3241 tatgagaacc gaaaacggta agtctaaggg ttttggtttt gtttgtttct caactccaga
     3301 ggaagctact aaggccatta cagaaaagaa ccaacaaatt gttgctggta agccattata
     3361 cgttgccatt gctcaaagaa aagacgtaag acgttctcaa ttggctcaac aaatccaagc
     3421 cagaaatcaa atgagatacc agcaagctac tgctgccgct gccgccgccg ctgccggtat
     3481 gccaggtcaa ttcatgcctc caatgttcta tggtgttatg ccaccaagag gtgttccatt
     3541 caacggtcca aacccacaac aaatgaaccc aatgggcggt atgccaaaga acggcatgcc
     3601 acctcaattt agaaatggtc cggtttacgg cgtcccccca caaggtggtt tcccaagaaa
     3661 tgccaacgat aacaaccaat tttatcaaca aaagcaaaga caagctttgg gtgaacaatt
     3721 atacaagaag gtttctgcta agacttcaaa tgaagaagca gctggtaaaa ttactggtat
     3781 gattttggat ttgccacctc aagaggtctt cccattgttg gaaagtgatg aattgttcga
     3841 acaacactac aaagaagctt ctgctgccta tgagtctttc aaaaaggagc aagaacaaca
     3901 aactgagcaa gctggcgcgc cgtaatagaa gcttgtcgag aagtactaga ggatcataat
     3961 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     4021 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     4081 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4141 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact
     4201 gcttgagcct aggagatccg aaccagataa gtgaaagcct gcatccgcag atcttaatta
     4261 ataaaacacg atacattgtt attagtacat ttattaagcg ctagattctg tgcgttgttg
     4321 atttacagac aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg
     4381 gtatgttaga gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa
     4441 atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga
     4501 accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg
     4561 tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac
     4621 gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg
     4681 actcgacgta aacacgttaa ataacgcttg gacatattta acatcgggcg tgttagcttt
     4741 attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga
     4801 ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt
     4861 tgtagttgag ctttttgggc ctcatgggcc ttccgctcac tgcccgcttt ccagtcggga
     4921 aacctgtcgt gccagctgca ttaacatggt catagctgtt tccttgcgta ttgggcgctc
     4981 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc gggtaaagcc tggggtgcct
     5041 aatgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5101 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5161 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5221 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5281 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5341 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5401 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5461 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5521 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5581 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     5641 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5701 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5761 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5821 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5881 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5941 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6001 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6061 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     6181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     6481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatta
     6721 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6781 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6841 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     6901 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     6961 agaccgagat agggttgagt ggccgctaca gggcgctccc attcgccatt caggctgcgc
     7021 aactgttggg aagggcgttt cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     7081 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     7141 taaaacgacg gccagtgagc gcgacgtaat acgactcact atagggcgaa ttggcggaag
     7201 gccgtcaagg ccgtgacgtt aaaactatta agccatccaa tcgaccgtta gtcgaatcag
     7261 gaccgctggt gcgagaagcc gcgaagtatg gcgaatgcat cgtataacgt gtggagtccg
     7321 ctcattagag cgtcatgttt agacaagaaa gctacatatt taattgatcc cgatgatttt
     7381 attgataaat tgaccctaac tccatacacg gtattctaca atggcggggt tttggtcaaa
     7441 atttccggac tgcgattgta catgctgtta acggctccgc ccactattaa tgaaattaaa
     7501 aattccaatt ttaaaaaacg cagcaagaga aacatttgta tgaaagaatg cgtagaagga
     7561 aagaaaaatg tcgtggacat gctgaacaac aagattaata tgcctccgtg tataaaaaaa
     7621 atattgaacg atttgaaaga aaacaatgta ccgcgcggcg gtatgtacag gaagaggttt
     7681 atactaaact gttacattgc aaacgtggtt tcgtgtgcca agtgtgaaaa ccgatgttta
     7741 atcaaggctc tgacgcattt ctacaaccac gactccaagt gtgtgggtga agtcatgcat
     7801 cttttaatca aatcccaaga tgtgtataaa ccaccaaact gccaaaaaat gaaaactgtg
     7861 gacaagctct gtccgtttgc tggcaactgc aagggtctca atcctatttg taattattga
     7921 ataataaaac aattataaat gctaaatttg ttttttatta acgatacaaa ccaaacgcaa
     7981 caagaacatt tgtagtatta tctataattg aaaacgcgta gttataatcg ctgaggtaat
     8041 atttaaaatc attttcaaat gattcacagt taatttgcga caatataatt ttattttcac
     8101 ataaactaga cgccttgtcg tcttcttctt cgtattcctt ctctttttca tttttctcct
     8161 cataaaaatt aacatagtta ttatcgtatc catatatgta tctatcgtat agagtaaatt
     8221 ttttgttgtc ataaatatat atgtcttttt taatggggtg tatagtaccg ctgcgcatag
     8281 tttttctgta atttacaaca gtgctatttt ctggtagttc ttcggagtgt gttgctttaa
     8341 ttattaaatt tatataatca atgaatttgg gatcgtcggt tttgtacaat atgttgccgg
     8401 catagtacgc agcttcttct agttcaatta caccattttt tagcagcacc ggattaacat
     8461 aactttccaa aatgttgtac gaaccgttaa acaaaaacag ttcacctccc ttttctatac
     8521 tattgtctgc gagcagttgt ttgttgttaa aaataacagc cattgtaatg agacgcacaa
     8581 actaatatca ca
//
