LOCUS       pOCC120-MBP-Ded1        8706 bp    DNA     circular UNA 08-APR-2024
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...5c-hkp70ad
VERSION     urn.local...5c-hkp70ad
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    38..57
                     /standard_name="AP00102-pOM1-seq.F1"
     promoter        57..151
     misc_feature    81..169
                     /standard_name="PH_promoter"
     misc_feature    174..201
                     /standard_name="POEM1-F primer"
     misc_feature    174..196
                     /standard_name="AP00103-pOM1-seq.F2"
     exon            224..1366
                     /standard_name="MBP from pMAL-c5G"
     misc_feature    complement(286..308)
                     /standard_name="AP0267-MBP-nR"
     misc_feature    1289..1309
                     /standard_name="AP0268-MBP-cF"
     primer_bind     1315..1332
                     /standard_name="Seq-pOCC120-fw"
     misc_feature    1379..1402
                     /standard_name="PreScission(1)"
     misc_feature    1405..1408
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    <1405..1410
                     /standard_name="Not1"
     misc_feature    1412..1819
                     /standard_name="disordered"
     misc_feature    1502..1507
                     /standard_name="BglII"
     misc_feature    1505..1663
                     /standard_name="Prion-like"
     misc_feature    1844..2908
                     /standard_name="Dexdc-helicc: just as an info to not cut
                     in here!"
     primer_bind     1941..1961
                     /standard_name="Seq-Ded1-fw"
     misc_feature    complement(2013..2014)
                     /standard_name="se till here"
     primer_bind     2085..2106
                     /standard_name="Ded1∆C-fw"
     misc_feature    2117..2122
                     /standard_name="SacI"
     misc_feature    2646
                     /standard_name="seq rev till here"
     misc_feature    complement(2962..2986)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Ded1∆C-rev (wo asc1) (reversed)"
     misc_feature    2987..3223
                     /standard_name="Disordered"
     misc_feature    3224..>3229
                     /standard_name="Asc1"
     misc_feature    3226..3229
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    3233..3262
                     /standard_name="linker"
     misc_feature    join(3263..3880,3884..3979)
                     /standard_name="eGFP mammalian"
     misc_feature    3263..3283
                     /standard_name="AP0015.hGFP/Cherry-nF"
     misc_feature    complement(3263..3283)
                     /standard_name="AP0017.hGFP/Cherry-nR"
     misc_feature    complement(3329..3346)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Seq-pOCC120-rev"
     misc_feature    3567..3586
                     /standard_name="AP0023.hGFP-mid-F"
     misc_feature    complement(3665..3684)
                     /standard_name="AP0024.hGFP-mid-R"
     misc_feature    3881..3883
                     /standard_name="A206K"
     misc_feature    3959..3979
                     /standard_name="AP0016.hGFP/Cherry-cF"
     misc_feature    complement(3959..3979)
                     /standard_name="AP0018.hGFP/Cherry-cR"
     misc_feature    3980..4003
                     /standard_name="PreScission"
     misc_feature    4013..4030
                     /standard_name="6xHis"
     misc_feature    4077..4107
                     /standard_name="POEM1-R primer"
     polyA_signal    complement(4111..4302)
     polyA_site      4203..4237
     misc_feature    complement(4250..4272)
                     /standard_name="AP00104-pOM1-seq.R"
     rep_origin      complement(5195..5877)
     CDS             complement(5972..6832)
                     /standard_name="AmpR"
     misc_feature    5972..5999
                     /standard_name="AP00120-Amp-C-R"
     misc_feature    complement(5975..5993)
                     /standard_name="AP0049.Amp-CF"
     misc_feature    complement(5978..6003)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Ded1∆C-rev (wo asc1)"
     misc_feature    complement(6813..6832)
                     /standard_name="AP0052.Amp-nF"
     misc_feature    6813..6832
                     /standard_name="AP0053.Amp-nR"
     promoter        complement(6868..6903)
     misc_feature    7231..7248
                     /standard_name="AP00111-M13(AP_design)"
     misc_feature    complement(7239..7257)
                     /standard_name="AP0261-sgRNA_seq-F"
     primer_bind     7253..7270
                     /standard_name="M13-fwd"
     misc_feature    7253..7270
                     /standard_name="AP00110-M13(18mer)"
     primer_bind     7280..7300
                     /standard_name="T7"
     misc_feature    7281..7300
                     /standard_name="AP00109-T7-promoter"
ORIGIN      
        1 agctgccacc attttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtat
       61 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
      121 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
      181 attcataccg tcccaccatc gggcgcggat cgccaccatg ggcatgaaaa tcgaagaagg
      241 taaactggta atctggatta acggcgataa aggctataac ggtctcgctg aagtcggtaa
      301 gaaattcgag aaagataccg gaattaaagt caccgttgag catccggata aactggaaga
      361 gaaattccca caggttgcgg caactggcga tggccctgac attatcttct gggcacacga
      421 ccgctttggt ggctacgctc aatctggcct gttggctgaa atcaccccgg acaaagcgtt
      481 ccaggacaag ctgtatccgt ttacctggga tgccgtacgt tacaacggca agctgattgc
      541 ttacccgatc gctgttgaag cgttatcgct gatttataac aaagatctgc tgccgaaccc
      601 gccaaaaacc tgggaagaga tcccggcgct ggataaagaa ctgaaagcga aaggtaagag
      661 cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg ccgctgattg ctgctgacgg
      721 gggttatgcg ttcaagtatg aaaacggcaa gtacgacatt aaagacgtgg gcgtggataa
      781 cgctggcgcg aaagcgggtc tgaccttcct ggttgacctg attaaaaaca aacacatgaa
      841 tgcagacacc gattactcca tcgcagaagc tgcctttaat aaaggcgaaa cagcgatgac
      901 catcaacggc ccgtgggcat ggtccaacat cgacaccagc aaagtgaatt atggtgtaac
      961 ggtactgccg accttcaagg gtcaaccatc caaaccgttc gttggcgtgc tgagcgcagg
     1021 tattaacgcc gccagtccga acaaagagct ggcaaaagag ttcctcgaaa actatctgct
     1081 gactgatgaa ggtctggaag cggttaataa agacaaaccg ctgggtgccg tagcgctgaa
     1141 gtcttacgag gaagagttgg tgaaagatcc gcgtattgcc gccactatgg aaaacgccca
     1201 gaaaggtgaa atcatgccga acatcccgca gatgtccgct ttctggtatg ccgtgcgtac
     1261 tgcggtgatc aacgccgcca gcggtcgtca gactgtcgat gaagccctga aagacgcgca
     1321 gactaattcg agctcgaaca acaacaacaa taacaataac aacaacagca gcggcagact
     1381 ggaagttctg ttccaggggc cggcggccgc aatggctgaa ctgtcagaac aggtacaaaa
     1441 cctgagtatc aatgataaca atgaaaatgg atacgttccg ccgcatttgc gcggaaagcc
     1501 gagatctgca cgcaataact catccaatta taacaacaac aatggtggtt acaatggtgg
     1561 aagaggcgga ggttcctttt tttcaaacaa tcgccgtggc ggatatggta acggtggatt
     1621 ttttggcggt aataatggcg gatccaggag taatggcaga tcaggtggtc gttggatcga
     1681 tggcaagcac gttcctgcgc cacgtaacga gaaggctgaa attgcgatct tcggagttcc
     1741 cgaggatcca aatttccaat catccggaat taactttgac aactacgatg acatcccggt
     1801 tgatgcctca ggaaaagatg tccctgaacc gataaccgaa tttacgtctc cccctttgga
     1861 cggtttgttg ctggagaaca tcaaactcgc tcgttttacc aagccaacac ccgtacagaa
     1921 atattctgtc ccgattgtag ctaacggtag agacttgatg gcatgcgctc aaacaggatc
     1981 cggcaagacg ggcggatttc tgtttcctgt gctgtctgaa tcattcaaaa ctggacccag
     2041 cccccaaccc gaaagtcagg gctcatttta ccaaagaaag gcgtacccga cagctgtgat
     2101 tatggctccc acgcgcgagc tcgcaactca gatattcgac gaggcgaaaa aatttacata
     2161 cagatcatgg gttaaggcat gcgtagtata cggcggtagt cctattggaa accagctcag
     2221 agagatagag agaggctgtg accttctggt ggcgactccc ggacgcctga atgatttgct
     2281 tgaaagggga aaaataagtc tcgcaaacgt gaaatacctt gtacttgatg aggctgacag
     2341 gatgctcgat atgggctttg aacctcaaat caggcatatt gtcgaagatt gcgacatgac
     2401 gcccgttgga gagagacaaa cattgatgtt cagtgcaaca tttccagcag acatccagca
     2461 tcttgcccgt gatttcctgt ccgattacat ctttctgtca gtcggcagag ttggctccac
     2521 gtctgagaac atcacacaga aggttttgta cgtcgagaat caagataaaa aatcagcttt
     2581 gttggacttg ttgagtgcat cgaccgacgg tctgacactt atttttgtag aaactaagag
     2641 aatggctgat caacttacag acttcttgat tatgcagaac tttcgcgcca cagccatcca
     2701 cggtgatcgc acacaatccg agcgcgagag ggcattggcg gcctttcgca gcggcgcggc
     2761 tactctgttg gtagctacag ctgtcgctgc tcgcggattg gacattccta atgtcaccca
     2821 tgttataaat tatgatttgc catccgatgt agacgattat gtgcatcgta tcggcagaac
     2881 tggaagagcc ggaaatacgg gactcgccac tgcatttttt aattcagaaa actccaatat
     2941 tgtgaagggc ctccacgaga tcctgacaga ggctaaccaa gaagtgccgt ctttcctgaa
     3001 ggatgctatg atgagcgccc ccggatcgcg ttctaacagt aggcgcggag gtttcggacg
     3061 caataacaat agagactacc gtaaagcagg aggtgcgtcc gcaggaggat ggggatcaag
     3121 tcgttctagg gataactcct ttaggggcgg tagtggttgg ggtagtgatt ccaagagttc
     3181 aggttggggt aattcgggcg gatccaacaa ctcttcatgg tggggcgcgc cgggatccgc
     3241 tggctccgct gctggttctg gcatggtgag caagggcgag gagctgttca ccggggtggt
     3301 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     3361 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     3421 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     3481 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     3541 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     3601 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     3661 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     3721 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     3781 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     3841 cgtgctgctg cccgacaacc actacctgag cacccagtcc aaactgagca aagaccccaa
     3901 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     3961 catggacgag ctgtacaagc tggaagttct gttccagggg ccgggcagca gccatcacca
     4021 tcaccatcac agtggataat agaagcttgt cgagaagtac tagaggatca taatcagcca
     4081 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     4141 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     4201 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     4261 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgcttga
     4321 gcctaggaga tccgaaccag ataagtgaaa gcctgcatcc gcagatctta attaataaaa
     4381 cacgatacat tgttattagt acatttatta agcgctagat tctgtgcgtt gttgatttac
     4441 agacaattgt tgtacgtatt ttaataattc attaaattta taatctttag ggtggtatgt
     4501 tagagcgaaa atcaaatgat tttcagcgtc tttatatctg aatttaaata ttaaatcctc
     4561 aatagatttg taaaataggt ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat
     4621 ggctggacta tctaatggat tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag
     4681 caatctagct ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt
     4741 caaaatatta tgcgcttttg tatttctttc atcactgtcg ttagtgtaca attgactcga
     4801 cgtaaacacg ttaaataacg cttggacata tttaacatcg ggcgtgttag ctttattagg
     4861 ccgattatcg tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag acgattttgc
     4921 catagccaca cgacgcctat taattgtgtc ggctaacacg tccgcgatca aatttgtagt
     4981 tgagcttttt gggcctcatg ggccttccgc tcactgcccg ctttccagtc gggaaacctg
     5041 tcgtgccagc tgcattaaca tggtcatagc tgtttccttg cgtattgggc gctctccgct
     5101 tcctcgctca ctgactcgct gcgctcggtc gttcgggtaa agcctggggt gcctaatgag
     5161 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5221 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5281 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5341 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5401 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5461 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5521 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5581 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5641 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5701 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5761 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5821 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5881 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5941 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6001 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6061 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6121 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6181 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6241 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
     6301 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6361 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6421 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6481 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6541 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     6601 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6661 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6721 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6781 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc attactcttc
     6841 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6901 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6961 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     7021 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     7081 agatagggtt gagtggccgc tacagggcgc tcccattcgc cattcaggct gcgcaactgt
     7141 tgggaagggc gtttcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     7201 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     7261 gacggccagt gagcgcgacg taatacgact cactataggg cgaattggcg gaaggccgtc
     7321 aaggccgtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     7381 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     7441 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     7501 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     7561 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     7621 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     7681 aatgtcgtgg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     7741 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     7801 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     7861 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     7921 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtggacaag
     7981 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     8041 aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     8101 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     8161 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     8221 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     8281 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     8341 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     8401 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     8461 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     8521 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     8581 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     8641 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     8701 atcaca
//
