LOCUS       pUC57-T7-PAB1-NL        3235 bp    DNA     circular UNA 08-APR-2024
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...5n-hkp7bbf
VERSION     urn.local...5n-hkp7bbf
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     source          join(1..>400,<1092..3235)
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    289..305
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name=2048
     primer_bind     379..395
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /standard_name="M13 fwd primer bind"
     misc_feature    397..400
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     unsure          <397..401
     misc_feature    397..553
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CAAACAAACAACTCAAAACAAAAAAAACTAAATACAAC"
     misc_feature    complement(402..421)
                     /standard_name="T7 Promoter"
     5'UTR           422..554
                     /standard_name="PAB1"
     misc_feature    555..1070
                     /standard_name="NanoLuc"
     misc_feature    1067^1068
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="AATTCTCATGGTTTTCCTCCTGAAGTTGAAGAACAAGCTGCT
                     GGTACTTTACCTATGTCTTGTGCTCAAGAATCTGGTATGGATCGTCATCCTGCTGCTT
                     GTGCTTCTGCTCGTATTAATGTT"
     source          <1071..>1090
                     /organism="pAG304GPD-ccdB-EGFP"
                     /mol_type="other DNA"
     misc_feature    1071..>1095
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     terminator      1084..>1090
     unsure          1091..>1095
     misc_feature    1092..1095
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     primer_bind     complement(1145..1161)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /standard_name="M13 rev primer bind"
     protein_bind    1169..1185
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /standard_name="lac operator protein bind"
     promoter        complement(1193..1223)
     misc_feature    complement(1233..1252)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name=2049
     rep_origin      complement(1547..2135)
     CDS             complement(2313..3122)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
                     GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /standard_name="aph(3')-Ia CDS"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ctaatacgac tcactatagg
      421 gagtaacatt tttacttaat aaccgtttga agcatagagc aggccctggt accaccacct
      481 aatatctggc tttttattca ataaaaactc aaaaaaaaaa atccaaaaaa aactaaaaaa
      541 ccaataaaaa taaaatggtt tttactttag aagattttgt tggtgattgg cgtcaaactg
      601 caggttataa tttagatcaa gttttagaac aaggtggtgt ttcttctttg tttcaaaact
      661 taggtgtttc tgttactcct attcaacgta ttgttttatc tggtgaaaat ggtttgaaga
      721 ttgatattca tgttattatt ccatatgaag gtttgtctgg tgatcaaatg ggtcaaattg
      781 aaaagatttt caaagttgtt tatcctgtcg acgatcatca tttcaaagtt attttgcatt
      841 atggtacttt agttattgat ggtgttactc ctaatatgat tgattatttt ggtcgtcctt
      901 atgaaggtat tgctgttttc gatggtaaaa agatcactgt tactggtact ttatggaatg
      961 gtaacaagat tattgatgaa cgtttgatta atcctgatgg ttctttatta tttcgtgtta
     1021 ctattaatgg tgttactggt tggcgtttat gtgaacgtat tttagcttaa ctcgagtcat
     1081 gtaattagtt ggatcccggg cccgtcgact gcagaggcct gcatgcaagc ttggcgtaat
     1141 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     1201 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     1261 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     1321 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     1381 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1441 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1501 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1561 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1621 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1681 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1741 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1801 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1861 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1921 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1981 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2041 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2101 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2161 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2221 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca agcccaatct
     2281 gaataatgtt acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga
     2341 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     2401 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     2461 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     2521 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta
     2581 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     2641 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg
     2701 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     2761 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttt
     2821 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     2881 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     2941 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     3001 aagcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     3061 aaatcagcat ccatgttgga atttaatcgc ggcctcgacg tttcccgttg aatatggctc
     3121 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata
     3181 tttttatctt gtgcaatgta acatcagaga ttttgagaca cgggccagag ctgca
//
