LOCUS       Exported                8880 bp ds-DNA     linear   UNA 18-JAN-2019
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 16, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8880
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    38..57
                     /locus_tag="AP00102-pOM1-seq.F1"
                     /label=AP00102-pOM1-seq.F1
     promoter        57..151
                     /locus_tag="B2E2_promoter"
                     /label=B2E2_promoter
     misc_feature    81..169
                     /locus_tag="PH_promoter"
                     /label=PH_promoter
     misc_feature    174..201
                     /locus_tag="POEM1-F primer"
                     /label=POEM1-F primer
     misc_feature    174..196
                     /locus_tag="AP00103-pOM1-seq.F2"
                     /label=AP00103-pOM1-seq.F2
     exon            224..1366
                     /locus_tag="MBP from pMAL-c5G"
                     /label=MBP from pMAL-c5G
     misc_feature    complement(286..308)
                     /locus_tag="AP0267-MBP-nR"
                     /label=AP0267-MBP-nR
     misc_feature    1289..1309
                     /locus_tag="AP0268-MBP-cF"
                     /label=AP0268-MBP-cF
     misc_feature    1379..1402
                     /locus_tag="PreScission(1)"
                     /label=PreScission
     misc_feature    1403..1410
                     /locus_tag="NotI"
                     /label=NotI
     misc_feature    1411..3397
                     /locus_tag="DDX3X_opt_Sf9"
                     /label=DDX3X_opt_Sf9
     misc_feature    1630..1635
                     /locus_tag="XhoI"
                     /label=XhoI
     misc_feature    1738..1743
                     /locus_tag="SacII"
                     /label=SacII
     misc_feature    2288..2293
                     /locus_tag="XbaI"
                     /label=XbaI
     misc_feature    2324..2329
                     /locus_tag="EcoRV"
                     /label=EcoRV
     misc_feature    2384..2386
                     /locus_tag="G325E"
                     /label=G325E
     misc_feature    2642..2647
                     /locus_tag="SpeI"
                     /label=SpeI
     misc_feature    3398..3405
                     /locus_tag="AscI"
                     /label=AscI
     misc_feature    3407..3436
                     /locus_tag="linker"
                     /label=linker
     misc_feature    join(3437..4054,4058..4153)
                     /locus_tag="mono eGFP (A206K) mammalian"
                     /label=mono eGFP (A206K) mammalian
     misc_feature    3437..3457
                     /locus_tag="AP0015.hGFP/Cherry-nF"
                     /label=AP0015.hGFP/Cherry-nF
     misc_feature    complement(3437..3457)
                     /locus_tag="AP0017.hGFP/Cherry-nR"
                     /label=AP0017.hGFP/Cherry-nR
     misc_feature    3741..3760
                     /locus_tag="AP0023.hGFP-mid-F"
                     /label=AP0023.hGFP-mid-F
     misc_feature    complement(3839..3858)
                     /locus_tag="AP0024.hGFP-mid-R"
                     /label=AP0024.hGFP-mid-R
     misc_feature    4055..4057
                     /locus_tag="A206K"
                     /label=A206K
     misc_feature    4133..4153
                     /locus_tag="AP0016.hGFP/Cherry-cF"
                     /label=AP0016.hGFP/Cherry-cF
     misc_feature    complement(4133..4153)
                     /locus_tag="AP0018.hGFP/Cherry-cR"
                     /label=AP0018.hGFP/Cherry-cR
     misc_feature    4154..4177
                     /locus_tag="PreScission"
                     /label=PreScission
     misc_feature    4187..4204
                     /locus_tag="6xHis"
                     /label=6xHis
     misc_feature    4251..4281
                     /locus_tag="POEM1-R primer"
                     /label=POEM1-R primer
     polyA_signal    complement(4285..4476)
                     /locus_tag="SV40 late polyA"
                     /label=SV40 late polyA
     polyA_site      4377..4411
                     /locus_tag="SV40 polyA"
                     /label=SV40 polyA
     misc_feature    complement(4424..4446)
                     /locus_tag="AP00104-pOM1-seq.R"
                     /label=AP00104-pOM1-seq.R
     rep_origin      complement(5369..6051)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    complement(5437..5443)
                     /locus_tag="branch site(1)"
                     /label=branch site
     CDS             complement(6146..7006)
                     /locus_tag="AmpR"
                     /label=AmpR
     misc_feature    6146..6173
                     /locus_tag="AP00120-Amp-C-R"
                     /label=AP00120-Amp-C-R
     misc_feature    complement(6149..6167)
                     /locus_tag="AP0049.Amp-CF"
                     /label=AP0049.Amp-CF
     misc_feature    complement(6987..7006)
                     /locus_tag="AP0052.Amp-nF"
                     /label=AP0052.Amp-nF
     misc_feature    6987..7006
                     /locus_tag="AP0053.Amp-nR"
                     /label=AP0053.Amp-nR
     promoter        complement(7042..7077)
                     /locus_tag="Bact.prom. from pEGFP-N3"
                     /label=Bact.prom. from pEGFP-N3 promoter
     misc_feature    complement(7344..7362)
                     /locus_tag="subcloning(PhiC)-R"
                     /label=subcloning(PhiC)-R
     misc_feature    7405..7422
                     /locus_tag="AP00111-M13(AP_design)"
                     /label=AP00111-M13(AP_design)
     misc_feature    complement(7413..7431)
                     /locus_tag="AP0261-sgRNA_seq-F"
                     /label=AP0261-sgRNA_seq-F
     misc_feature    7427..7444
                     /locus_tag="AP00110-M13(18mer)"
                     /label=AP00110-M13(18mer)
     primer_bind     7427..7444
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     primer_bind     7454..7474
                     /locus_tag="T7"
                     /label=T7
     misc_feature    7455..7474
                     /locus_tag="AP00109-T7-promoter"
                     /label=AP00109-T7-promoter
     misc_feature    8036..8042
                     /locus_tag="branch site"
                     /label=branch site
ORIGIN
        1 agctgccacc attttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtat
       61 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
      121 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
      181 attcataccg tcccaccatc gggcgcggat cgccaccatg ggcatgaaaa tcgaagaagg
      241 taaactggta atctggatta acggcgataa aggctataac ggtctcgctg aagtcggtaa
      301 gaaattcgag aaagataccg gaattaaagt caccgttgag catccggata aactggaaga
      361 gaaattccca caggttgcgg caactggcga tggccctgac attatcttct gggcacacga
      421 ccgctttggt ggctacgctc aatctggcct gttggctgaa atcaccccgg acaaagcgtt
      481 ccaggacaag ctgtatccgt ttacctggga tgccgtacgt tacaacggca agctgattgc
      541 ttacccgatc gctgttgaag cgttatcgct gatttataac aaagatctgc tgccgaaccc
      601 gccaaaaacc tgggaagaga tcccggcgct ggataaagaa ctgaaagcga aaggtaagag
      661 cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg ccgctgattg ctgctgacgg
      721 gggttatgcg ttcaagtatg aaaacggcaa gtacgacatt aaagacgtgg gcgtggataa
      781 cgctggcgcg aaagcgggtc tgaccttcct ggttgacctg attaaaaaca aacacatgaa
      841 tgcagacacc gattactcca tcgcagaagc tgcctttaat aaaggcgaaa cagcgatgac
      901 catcaacggc ccgtgggcat ggtccaacat cgacaccagc aaagtgaatt atggtgtaac
      961 ggtactgccg accttcaagg gtcaaccatc caaaccgttc gttggcgtgc tgagcgcagg
     1021 tattaacgcc gccagtccga acaaagagct ggcaaaagag ttcctcgaaa actatctgct
     1081 gactgatgaa ggtctggaag cggttaataa agacaaaccg ctgggtgccg tagcgctgaa
     1141 gtcttacgag gaagagttgg tgaaagatcc gcgtattgcc gccactatgg aaaacgccca
     1201 gaaaggtgaa atcatgccga acatcccgca gatgtccgct ttctggtatg ccgtgcgtac
     1261 tgcggtgatc aacgccgcca gcggtcgtca gactgtcgat gaagccctga aagacgcgca
     1321 gactaattcg agctcgaaca acaacaacaa taacaataac aacaacagca gcggcagact
     1381 ggaagttctg ttccaggggc cggcggccgc aatgtcccac gtggcagttg agaacgcttt
     1441 gggcctggat caacagttcg ccggattgga cctcaactcg tcggacaacc aatcaggagg
     1501 ctccacggcc tcaaagggac gttacatccc gccacatctc cgcaataggg aggcaaccaa
     1561 aggcttctac gataaagatt cctcaggttg gtcgtcatca aaggataaag atgcttactc
     1621 atcgtttggc tcgaggtcag attcacgcgg caaatccagc ttcttcagtg atcgcggatc
     1681 aggctcacgc ggaaggtttg acgacagggg acgttccgac tatgatggca taggttcccg
     1741 cggtgatcgc agtggctttg gcaaattcga aagaggtggc aattcgcgct ggtgtgacaa
     1801 atctgatgaa gacgactggt cgaaaccact tccgccatct gagagactgg agcaagagct
     1861 gttctcagga ggaaacaccg gaattaattt cgaaaagtac gacgatattc cagtagaagc
     1921 gacgggaaat aactgccctc cacacatcga aagctttagt gacgtcgaaa tgggagagat
     1981 catcatgggt aatatcgaac tcaccagata cactcgtcct actcctgttc aaaaacatgc
     2041 tattccaatt ataaaagaaa agcgtgatct tatggcatgt gcacaaacag gatctggtaa
     2101 gacagcagcc tttctcctgc caatcctttc acagatatac tcggatggcc ccggggaagc
     2161 tctccgtgct atgaaggaaa acggaaggta cggtcgcaga aaacagtatc ctatttcttt
     2221 ggtcctggcc cctacgaggg aactggcggt gcaaatttat gaggaagccc gcaaattttc
     2281 atataggtct agagttaggc cctgtgtcgt atacggagga gctgatatcg gacaacaaat
     2341 ccgtgacctt gaaaggggct gtcaccttct tgtcgccaca ccagaacgct tggtagatat
     2401 gatggaaaga ggaaaaatag gcttggactt ctgcaagtac ctcgtcctcg acgaagccga
     2461 ccgtatgctt gatatgggat ttgagcctca gatacgtcgc atagttgagc aagatacaat
     2521 gccgcctaag ggcgtcaggc acactatgat gttctcagcc acttttccga aggagattca
     2581 gatgttggcc cgtgatttcc tggacgaata catattcctc gccgtcggaa gagtcggtag
     2641 tactagtgaa aatatcaccc aaaaagtcgt ttgggttgag gaaagcgaca aacgtagttt
     2701 ccttcttgat ctgttgaatg ctacgggtaa agacagtctt acactggtgt ttgttgagac
     2761 taaaaaggga gctgactccc ttgaagactt tctgtatcat gaaggatatg cttgtacgtc
     2821 catacatgga gatcgctctc aaagggaccg tgaagaggcg ctccatcaat tccgctccgg
     2881 caagagccca atcttggttg ctaccgcagt cgcggctagg ggtttggata ttagcaatgt
     2941 taagcatgtt atcaacttcg atctccccag cgatatagaa gagtatgttc accgcatcgg
     3001 acgcacaggt cgtgtgggca accttggact ggccacgagt ttcttcaacg aaagaaatat
     3061 taatataacg aaagatctgc tcgacctgct tgtcgaagct aaacaggaag taccatcctg
     3121 gcttgaaaat atggcgtatg aacaccatta taaaggctca tcacgtggaa ggtcaaagag
     3181 tagcaggttt tcgggcggct ttggtgccag agactaccgc cagtcatcgg gtgcaagttc
     3241 gagctccttc tcttccagtc gcgcatcatc tagtcgtagc ggtggcggag gtcatggaag
     3301 cagcagggga ttcggcggag gtggctatgg tggcttctat aattcagacg gctacggagg
     3361 aaactacaac tcgcaaggtg tagactggtg gggaaacggc gcgccgggat ccgctggctc
     3421 cgctgctggt tctggcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     3481 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     3541 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     3601 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     3661 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     3721 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     3781 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     3841 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     3901 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     3961 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     4021 gctgcccgac aaccactacc tgagcaccca gtccaaactg agcaaagacc ccaacgagaa
     4081 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     4141 cgagctgtac aagctggaag ttctgttcca ggggccgggc agcagccatc accatcacca
     4201 tcacagtgga taatagaagc ttgtcgagaa gtactagagg atcataatca gccataccac
     4261 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     4321 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     4381 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4441 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag
     4501 gagatccgaa ccagataagt gaaagcctgc atccgcagat cttaattaat aaaacacgat
     4561 acattgttat tagtacattt attaagcgct agattctgtg cgttgttgat ttacagacaa
     4621 ttgttgtacg tattttaata attcattaaa tttataatct ttagggtggt atgttagagc
     4681 gaaaatcaaa tgattttcag cgtctttata tctgaattta aatattaaat cctcaataga
     4741 tttgtaaaat aggtttcgat tagtttcaaa caagggttgt ttttccgaac cgatggctgg
     4801 actatctaat ggattttcgc tcaacgccac aaaacttgcc aaatcttgta gcagcaatct
     4861 agctttgtcg atattcgttt gtgttttgtt ttgtaataaa ggttcgacgt cgttcaaaat
     4921 attatgcgct tttgtatttc tttcatcact gtcgttagtg tacaattgac tcgacgtaaa
     4981 cacgttaaat aacgcttgga catatttaac atcgggcgtg ttagctttat taggccgatt
     5041 atcgtcgtcg tcccaaccct cgtcgttaga agttgcttcc gaagacgatt ttgccatagc
     5101 cacacgacgc ctattaattg tgtcggctaa cacgtccgcg atcaaatttg tagttgagct
     5161 ttttgggcct catgggcctt ccgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5221 cagctgcatt aacatggtca tagctgtttc cttgcgtatt gggcgctctc cgcttcctcg
     5281 ctcactgact cgctgcgctc ggtcgttcgg gtaaagcctg gggtgcctaa tgagcaaaag
     5341 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5401 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5461 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5521 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5581 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5641 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5701 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5761 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5821 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5881 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5941 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6001 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6061 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6121 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6181 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6241 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6301 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6361 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6421 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
     6481 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6541 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6601 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6661 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6721 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     6781 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6841 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6901 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6961 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcattact cttccttttt
     7021 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7081 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     7141 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     7201 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     7261 ggttgagtgg ccgctacagg gcgctcccat tcgccattca ggctgcgcaa ctgttgggaa
     7321 gggcgtttcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     7381 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     7441 cagtgagcgc gacgtaatac gactcactat agggcgaatt ggcggaaggc cgtcaaggcc
     7501 gtgacgttaa aactattaag ccatccaatc gaccgttagt cgaatcagga ccgctggtgc
     7561 gagaagccgc gaagtatggc gaatgcatcg tataacgtgt ggagtccgct cattagagcg
     7621 tcatgtttag acaagaaagc tacatattta attgatcccg atgattttat tgataaattg
     7681 accctaactc catacacggt attctacaat ggcggggttt tggtcaaaat ttccggactg
     7741 cgattgtaca tgctgttaac ggctccgccc actattaatg aaattaaaaa ttccaatttt
     7801 aaaaaacgca gcaagagaaa catttgtatg aaagaatgcg tagaaggaaa gaaaaatgtc
     7861 gtggacatgc tgaacaacaa gattaatatg cctccgtgta taaaaaaaat attgaacgat
     7921 ttgaaagaaa acaatgtacc gcgcggcggt atgtacagga agaggtttat actaaactgt
     7981 tacattgcaa acgtggtttc gtgtgccaag tgtgaaaacc gatgtttaat caaggctctg
     8041 acgcatttct acaaccacga ctccaagtgt gtgggtgaag tcatgcatct tttaatcaaa
     8101 tcccaagatg tgtataaacc accaaactgc caaaaaatga aaactgtgga caagctctgt
     8161 ccgtttgctg gcaactgcaa gggtctcaat cctatttgta attattgaat aataaaacaa
     8221 ttataaatgc taaatttgtt ttttattaac gatacaaacc aaacgcaaca agaacatttg
     8281 tagtattatc tataattgaa aacgcgtagt tataatcgct gaggtaatat ttaaaatcat
     8341 tttcaaatga ttcacagtta atttgcgaca atataatttt attttcacat aaactagacg
     8401 ccttgtcgtc ttcttcttcg tattccttct ctttttcatt tttctcctca taaaaattaa
     8461 catagttatt atcgtatcca tatatgtatc tatcgtatag agtaaatttt ttgttgtcat
     8521 aaatatatat gtctttttta atggggtgta tagtaccgct gcgcatagtt tttctgtaat
     8581 ttacaacagt gctattttct ggtagttctt cggagtgtgt tgctttaatt attaaattta
     8641 tataatcaat gaatttggga tcgtcggttt tgtacaatat gttgccggca tagtacgcag
     8701 cttcttctag ttcaattaca ccatttttta gcagcaccgg attaacataa ctttccaaaa
     8761 tgttgtacga accgttaaac aaaaacagtt cacctccctt ttctatacta ttgtctgcga
     8821 gcagttgttt gttgttaaaa ataacagcca ttgtaatgag acgcacaaac taatatcaca
//
