LOCUS       Exported                8112 bp ds-DNA     circular SYN 11-DEC-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 16, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     /Volumes/bogdanova/sequences/EXPRESSION 
            VECTORSPROJECT/DESTINATION/PreSci-Not-Asc/pOCC50_pOEM1-N-MBP-HIS6.a 
            pe from1 to 6550 
            /private/Network/Servers/fileserver.mpi-cbg.de/bogdanova/sequences/ 
            EXPRESSIONVECTORSPROJECT/DESTINATION/PreSci-Not-Asc/pOCC5_pOEM1-N-H 
            IS6.apefrom 1 to5404 
            /private/Network/Servers/fileserver.mpi-cbg.de/bogdanova/sequences/ 
            Vector/EXPRESSION/Baculovirus/TH0567-pOEM1.ape from 1 to 5008
FEATURES             Location/Qualifiers
     source          1..8112
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        57..151
                     /locus_tag="B2E2_promoter"
                     /label=B2E2_promoter
     misc_feature    81..169
                     /locus_tag="PH_promoter"
                     /label=PH_promoter
     misc_feature    230..247
                     /locus_tag="6xHis"
                     /label=6xHis
     exon            260..1402
                     /locus_tag="MBP from pMAL-c5G"
                     /label=MBP from pMAL-c5G
     misc_feature    1415..1438
                     /locus_tag="PreScission"
                     /label=PreScission
     misc_feature    1447..3433
                     /locus_tag="DDX3X_opt_Sf9"
                     /label=DDX3X_opt_Sf9
     misc_feature    1666..1671
                     /locus_tag="XhoI"
                     /label=XhoI
     misc_feature    1774..1779
                     /locus_tag="SacII"
                     /label=SacII
     misc_feature    2324..2329
                     /locus_tag="XbaI"
                     /label=XbaI
     misc_feature    2360..2365
                     /locus_tag="EcoRV"
                     /label=EcoRV
     misc_feature    2678..2683
                     /locus_tag="SpeI"
                     /label=SpeI
     polyA_signal    complement(3517..3708)
                     /locus_tag="SV40 late polyA"
                     /label=SV40 late polyA
     polyA_site      3609..3643
                     /locus_tag="SV40 polyA"
                     /label=SV40 polyA
     rep_origin      complement(4601..5283)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     CDS             complement(5378..6238)
                     /locus_tag="AmpR"
                     /label=AmpR
     misc_feature    complement(5381..5399)
                     /locus_tag="AP0049.Amp-CF"
                     /label=AP0049.Amp-CF
     misc_feature    complement(6219..6238)
                     /locus_tag="AP0052.Amp-nF"
                     /label=AP0052.Amp-nF
     misc_feature    6219..6238
                     /locus_tag="AP0053.Amp-nR"
                     /label=AP0053.Amp-nR
     promoter        complement(6274..6309)
                     /locus_tag="Bact.prom. from pEGFP-N3"
                     /label=Bact.prom. from pEGFP-N3
     primer_bind     6659..6676
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     primer_bind     6686..6706
                     /locus_tag="T7"
                     /label=T7
ORIGIN
        1 agctgccacc attttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtat
       61 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
      121 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
      181 attcataccg tcccaccatc gggcgcggat cgccaccatg ggcagcagcc atcaccatca
      241 ccatcacagc agcggcagaa tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg
      301 cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat
      361 taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac
      421 tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc
      481 tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac
      541 ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt
      601 atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc
      661 ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga
      721 accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa
      781 cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac
      841 cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc
      901 agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc
      961 caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca
     1021 accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa
     1081 agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt
     1141 taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggtgaa
     1201 agatccgcgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat
     1261 cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg
     1321 tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa
     1381 caacaataac aataacaaca acagcagcgg cagactggaa gttctgttcc aggggccggc
     1441 ggccgcaatg tcccacgtgg cagttgagaa cgctttgggc ctggatcaac agttcgccgg
     1501 attggacctc aactcgtcgg acaaccaatc aggaggctcc acggcctcaa agggacgtta
     1561 catcccgcca catctccgca atagggaggc aaccaaaggc ttctacgata aagattcctc
     1621 aggttggtcg tcatcaaagg ataaagatgc ttactcatcg tttggctcga ggtcagattc
     1681 acgcggcaaa tccagcttct tcagtgatcg cggatcaggc tcacgcggaa ggtttgacga
     1741 caggggacgt tccgactatg atggcatagg ttcccgcggt gatcgcagtg gctttggcaa
     1801 attcgaaaga ggtggcaatt cgcgctggtg tgacaaatct gatgaagacg actggtcgaa
     1861 accacttccg ccatctgaga gactggagca agagctgttc tcaggaggaa acaccggaat
     1921 taatttcgaa aagtacgacg atattccagt agaagcgacg ggaaataact gccctccaca
     1981 catcgaaagc tttagtgacg tcgaaatggg agagatcatc atgggtaata tcgaactcac
     2041 cagatacact cgtcctactc ctgttcaaaa acatgctatt ccaattataa aagaaaagcg
     2101 tgatcttatg gcatgtgcac aaacaggatc tggtaagaca gcagcctttc tcctgccaat
     2161 cctttcacag atatactcgg atggccccgg ggaagctctc cgtgctatga aggaaaacgg
     2221 aaggtacggt cgcagaaaac agtatcctat ttctttggtc ctggccccta cgagggaact
     2281 ggcggtgcaa atttatgagg aagcccgcaa attttcatat aggtctagag ttaggccctg
     2341 tgtcgtatac ggaggagctg atatcggaca acaaatccgt gaccttgaaa ggggctgtca
     2401 ccttcttgtc gccacaccag gacgcttggt agatatgatg gaaagaggaa aaataggctt
     2461 ggacttctgc aagtacctcg tcctcgacga agccgaccgt atgcttgata tgggatttga
     2521 gcctcagata cgtcgcatag ttgagcaaga tacaatgccg cctaagggcg tcaggcacac
     2581 tatgatgttc tcagccactt ttccgaagga gattcagatg ttggcccgtg atttcctgga
     2641 cgaatacata ttcctcgccg tcggaagagt cggtagtact agtgaaaata tcacccaaaa
     2701 agtcgtttgg gttgaggaaa gcgacaaacg tagtttcctt cttgatctgt tgaatgctac
     2761 gggtaaagac agtcttacac tggtgtttgt tgagactaaa aagggagctg actcccttga
     2821 agactttctg tatcatgaag gatatgcttg tacgtccata catggagatc gctctcaaag
     2881 ggaccgtgaa gaggcgctcc atcaattccg ctccggcaag agcccaatct tggttgctac
     2941 cgcagtcgcg gctaggggtt tggatattag caatgttaag catgttatca acttcgatct
     3001 ccccagcgat atagaagagt atgttcaccg catcggacgc acaggtcgtg tgggcaacct
     3061 tggactggcc acgagtttct tcaacgaaag aaatattaat ataacgaaag atctgctcga
     3121 cctgcttgtc gaagctaaac aggaagtacc atcctggctt gaaaatatgg cgtatgaaca
     3181 ccattataaa ggctcatcac gtggaaggtc aaagagtagc aggttttcgg gcggctttgg
     3241 tgccagagac taccgccagt catcgggtgc aagttcgagc tccttctctt ccagtcgcgc
     3301 atcatctagt cgtagcggtg gcggaggtca tggaagcagc aggggattcg gcggaggtgg
     3361 ctatggtggc ttctataatt cagacggcta cggaggaaac tacaactcgc aaggtgtaga
     3421 ctggtgggga aacggcgcgc cgtaatagaa gcttgtcgag aagtactaga ggatcataat
     3481 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     3541 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     3601 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3661 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact
     3721 gcttgagcct aggagatccg aaccagataa gtgaaagcct gcatccgcag atcttaatta
     3781 ataaaacacg atacattgtt attagtacat ttattaagcg ctagattctg tgcgttgttg
     3841 atttacagac aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg
     3901 gtatgttaga gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa
     3961 atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga
     4021 accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg
     4081 tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac
     4141 gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg
     4201 actcgacgta aacacgttaa ataacgcttg gacatattta acatcgggcg tgttagcttt
     4261 attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga
     4321 ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt
     4381 tgtagttgag ctttttgggc ctcatgggcc ttccgctcac tgcccgcttt ccagtcggga
     4441 aacctgtcgt gccagctgca ttaacatggt catagctgtt tccttgcgta ttgggcgctc
     4501 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc gggtaaagcc tggggtgcct
     4561 aatgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4621 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4681 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4741 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4801 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4861 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4921 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4981 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5041 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5101 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     5161 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5221 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5281 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5341 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5401 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5461 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     5521 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     5581 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     5641 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     5701 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     5761 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     5821 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     5881 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     5941 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     6001 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6061 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6121 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6181 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatta
     6241 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6301 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6361 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     6421 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     6481 agaccgagat agggttgagt ggccgctaca gggcgctccc attcgccatt caggctgcgc
     6541 aactgttggg aagggcgttt cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     6601 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     6661 taaaacgacg gccagtgagc gcgacgtaat acgactcact atagggcgaa ttggcggaag
     6721 gccgtcaagg ccgtgacgtt aaaactatta agccatccaa tcgaccgtta gtcgaatcag
     6781 gaccgctggt gcgagaagcc gcgaagtatg gcgaatgcat cgtataacgt gtggagtccg
     6841 ctcattagag cgtcatgttt agacaagaaa gctacatatt taattgatcc cgatgatttt
     6901 attgataaat tgaccctaac tccatacacg gtattctaca atggcggggt tttggtcaaa
     6961 atttccggac tgcgattgta catgctgtta acggctccgc ccactattaa tgaaattaaa
     7021 aattccaatt ttaaaaaacg cagcaagaga aacatttgta tgaaagaatg cgtagaagga
     7081 aagaaaaatg tcgtggacat gctgaacaac aagattaata tgcctccgtg tataaaaaaa
     7141 atattgaacg atttgaaaga aaacaatgta ccgcgcggcg gtatgtacag gaagaggttt
     7201 atactaaact gttacattgc aaacgtggtt tcgtgtgcca agtgtgaaaa ccgatgttta
     7261 atcaaggctc tgacgcattt ctacaaccac gactccaagt gtgtgggtga agtcatgcat
     7321 cttttaatca aatcccaaga tgtgtataaa ccaccaaact gccaaaaaat gaaaactgtg
     7381 gacaagctct gtccgtttgc tggcaactgc aagggtctca atcctatttg taattattga
     7441 ataataaaac aattataaat gctaaatttg ttttttatta acgatacaaa ccaaacgcaa
     7501 caagaacatt tgtagtatta tctataattg aaaacgcgta gttataatcg ctgaggtaat
     7561 atttaaaatc attttcaaat gattcacagt taatttgcga caatataatt ttattttcac
     7621 ataaactaga cgccttgtcg tcttcttctt cgtattcctt ctctttttca tttttctcct
     7681 cataaaaatt aacatagtta ttatcgtatc catatatgta tctatcgtat agagtaaatt
     7741 ttttgttgtc ataaatatat atgtcttttt taatggggtg tatagtaccg ctgcgcatag
     7801 tttttctgta atttacaaca gtgctatttt ctggtagttc ttcggagtgt gttgctttaa
     7861 ttattaaatt tatataatca atgaatttgg gatcgtcggt tttgtacaat atgttgccgg
     7921 catagtacgc agcttcttct agttcaatta caccattttt tagcagcacc ggattaacat
     7981 aactttccaa aatgttgtac gaaccgttaa acaaaaacag ttcacctccc ttttctatac
     8041 tattgtctgc gagcagttgt ttgttgttaa aaataacagc cattgtaatg agacgcacaa
     8101 actaatatca ca
//
