LOCUS       Exported                3178 bp ds-DNA     circular SYN 14-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 16, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    402..424
                     /locus_tag="T7_promoter"
                     /label=T7_promoter
     misc_feature    425..430
                     /locus_tag="SacI"
                     /label=SacI
     misc_feature    431..480
                     /locus_tag="5UTR_beta-globin"
                     /label=5UTR_beta-globin
     misc_feature    481..486
                     /locus_tag="PstI"
                     /label=PstI
     misc_feature    487..495
                     /locus_tag="Kozak"
                     /label=Kozak
     misc_feature    496..1069
                     /locus_tag="nanoLUC"
                     /label=nanoLUC
     misc_feature    1012..1069
                     /locus_tag="3UTR_MCS"
                     /label=3UTR_MCS
     misc_feature    1047..1069
                     /locus_tag="Short_polyA"
                     /label=Short_polyA
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt ctaatacgac tcactatagg
      421 gagagagctc acatttgctt ctgacacaac tgtgttcact agcaacctca aacagacacc
      481 ctgcaggccg ccaccatggt ttttacttta gaagattttg ttggtgattg gcgtcaaact
      541 gctggttata atttagatca agttttagaa caaggtggtg tttcttcttt gtttcaaaac
      601 ttaggtgttt ctgttactcc tattcaacgt attgttttat ctggtgaaaa tggtttgaag
      661 attgatattc atgttattat tccatatgaa ggtttgtctg gtgatcaaat gggtcaaatt
      721 gaaaagattt tcaaagttgt ttatcctgtg gacgatcatc atttcaaagt tattttgcat
      781 tatggtactt tagttattga tggtgttact cctaatatga ttgattattt tggtcgtcct
      841 tatgaaggta ttgctgtttt cgatggtaaa aagatcactg ttactggtac tttatggaat
      901 ggtaacaaga ttattgatga acgtttgatt aatcctgatg gttctttatt atttcgtgtt
      961 actattaatg gtgttactgg ttggcgttta tgtgaacgta ttttagctta agcggccgca
     1021 ccggtgctag ctcgagacta gtcgacaaaa aaaaaaaaaa aaaaaaaaaa agcttggtgt
     1081 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     1141 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     1201 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     1261 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     1321 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     1381 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     1441 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1501 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1561 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1621 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1681 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1741 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1801 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1861 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1921 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1981 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2041 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2101 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     2161 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaagcccaa
     2221 tctgaataat gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa
     2281 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     2341 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     2401 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     2461 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt
     2521 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     2581 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
     2641 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     2701 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     2761 tttccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     2821 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     2881 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     2941 tacaagcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     3001 tataaatcag catccatgtt ggaatttaat cgcggcctcg acgtttcccg ttgaatatgg
     3061 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     3121 atatttttat cttgtgcaat gtaacatcag agattttgag acacgggcca gagctgca
//
