LOCUS pcDNA3_mId1_IRES 7176 bp DNA circular 12-APR-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7181) AUTHORS . TITLE Direct Submission JOURNAL Exported Sunday, Apr 12, 2020 from SnapGene Viewer 5.0.8 https://www.snapgene.com COMMENT Created: Saturday, December 27, 2008 1:53 PM pcDNA3 COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 209..863 /note="CMV promoter" /locus_tag="CMV promoter" /label="CMV promoter" /ApEinfo_label="CMV promoter" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 916..1362 /note="Mouse Id1" /locus_tag="Mouse Id1" /label="Mouse Id1" /ApEinfo_label="Mouse Id1" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 2748..2979 /note="bGH pA" /locus_tag="bGH pA" /label="bGH pA" /ApEinfo_label="bGH pA" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 3520..3845 /note="SV40 promoter" /locus_tag="SV40 promoter" /label="SV40 promoter" /ApEinfo_label="SV40 promoter" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 3881..4675 /note="Neo ORF" /locus_tag="Neo ORF" /label="Neo ORF" /ApEinfo_label="Neo ORF" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 4730..5102 /note="SV40 pA" /locus_tag="SV40 pA" /label="SV40 pA" /ApEinfo_label="SV40 pA" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 1369..1992 /locus_tag="EMCV IRES" /label="EMCV IRES" /ApEinfo_label="EMCV IRES" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 1993..2712 /locus_tag="YFP" /label="YFP" /ApEinfo_label="YFP" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ccaccatgaa ggtcgccagt ggcagtgccg cagccgctgc aggccctagc 961 tgttcgctga aggcgggcag gacagcgggc gaggtggtac ttggtctgtc ggagcaaagc 1021 gtggccatct cgcgctgcgc tgggacgcgc ctgcccgcct tgctggacga gcagcaggtg 1081 aacgtcctgc tctacgacat gaacggctgc tactcacgcc tcaaggagct ggtgcccacc 1141 ctgccccaga accgcaaagt gagcaaggtg gagatcctgc agcatgtaat cgactacatc 1201 agggacctgc agctggagct gaactcggag tctgaagtcg ggaccaccgg aggccgggga 1261 ctgcctgtcc gcgccccgct cagcaccctg aacggcgaga tcagtgcctt ggcggccgag 1321 gcggcatgtg ttccagccga cgatcgcatc ttgtgtcgct gagaattcTG GCGAAAGGGG 1381 GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA 1441 AAACGACGGC CAGTGAATTG TAATACGACT CACTATAGGG CGAATTAATT CCGGTTATTT 1501 TCCACCATAT TGCCGTCTTT TGGCAATGTG AGGGCCCGGA AACCTGGCCC TGTCTTCTTG 1561 ACGAGCATTC CTAGGGGTCT TTCCCCTCTC GCCAAAGGAA TGCAAGGTCT GTTGAATGTC 1621 GTGAAGGAAG CAGTTCCTCT GGAAGCTTCT TGAAGACAAA CAACGTCTGT AGCGACCCTT 1681 TGCAGGCAGC GGAACCCCCC ACCTGGCGAC AGGTGCCTCT GCGGCCAAAA GCCACGTGTA 1741 TAAGATACAC CTGCAAAGGC GGCACAACCC CAGTGCCACG TTGTGAGTTG GATAGTTGTG 1801 GAAAGAGTCA AATGGCTCTC CTCAAGCGTA TTCAACAAGG GGCTGAAGGA TGCCCAGAAG 1861 GTACCCCATT GTATGGGATC TGATCTGGGG CCTCGGTGCA CATGCTTTAC ATGTGTTTAG 1921 TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA CGTGGTTTTC CTTTGAAAAA 1981 CACGATGATA CCatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 2041 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 2101 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg 2161 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc 2221 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 2281 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 2341 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 2401 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac 2461 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc 2521 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 2581 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc 2641 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg cATGGACGAG 2701 CTGTACAAGT AAtctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg 2761 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc 2821 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc 2881 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa 2941 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc 3001 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc 3061 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 3121 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 3181 tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga 3241 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 3301 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 3361 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tggggatttc 3421 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg 3481 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca 3541 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 3601 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 3661 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 3721 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg 3781 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat 3841 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 3901 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 3961 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 4021 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 4081 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 4141 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 4201 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 4261 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 4321 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 4381 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 4441 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 4501 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 4561 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 4621 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 4681 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 4741 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 4801 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt 4861 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 4921 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt 4981 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 5041 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 5101 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 5161 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 5221 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 5281 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 5341 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 5401 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 5461 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 5521 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 5581 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt 5641 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 5701 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 5761 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 5821 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 5881 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 5941 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 6001 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 6061 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 6121 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 6181 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 6241 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 6301 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 6361 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 6421 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 6481 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 6541 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 6601 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 6661 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 6721 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 6781 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 6841 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 6901 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 6961 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 7021 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 7081 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 7141 ccgcgcacat ttccccgaaa agtgccacct gacgtc //