LOCUS pcDNA3_mId1_BICE 7181 bp DNA circular 12-APR-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7181) AUTHORS . TITLE Direct Submission JOURNAL Exported Sunday, Apr 12, 2020 from SnapGene Viewer 5.0.8 https://www.snapgene.com COMMENT Created: Saturday, December 27, 2008 1:53 PM pcDNA3 COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 209..863 /note="CMV promoter" /locus_tag="CMV promoter" /label="CMV promoter" /ApEinfo_label="CMV promoter" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 916..1362 /note="Mouse Id1" /locus_tag="Mouse Id1" /label="Mouse Id1" /ApEinfo_label="Mouse Id1" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 2753..2984 /note="bGH pA" /locus_tag="bGH pA" /label="bGH pA" /ApEinfo_label="bGH pA" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 3525..3850 /note="SV40 promoter" /locus_tag="SV40 promoter" /label="SV40 promoter" /ApEinfo_label="SV40 promoter" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 3886..4680 /note="Neo ORF" /locus_tag="Neo ORF" /label="Neo ORF" /ApEinfo_label="Neo ORF" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 4735..5107 /note="SV40 pA" /locus_tag="SV40 pA" /label="SV40 pA" /ApEinfo_label="SV40 pA" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 1387..1997 /note="IRES" /locus_tag="BICEP IRES from Sigma" /label="BICEP IRES from Sigma" /ApEinfo_label="BICEP IRES from Sigma" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 1998..2717 /locus_tag="YFP" /label="YFP" /ApEinfo_label="YFP" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ccaccatgaa ggtcgccagt ggcagtgccg cagccgctgc aggccctagc 961 tgttcgctga aggcgggcag gacagcgggc gaggtggtac ttggtctgtc ggagcaaagc 1021 gtggccatct cgcgctgcgc tgggacgcgc ctgcccgcct tgctggacga gcagcaggtg 1081 aacgtcctgc tctacgacat gaacggctgc tactcacgcc tcaaggagct ggtgcccacc 1141 ctgccccaga accgcaaagt gagcaaggtg gagatcctgc agcatgtaat cgactacatc 1201 agggacctgc agctggagct gaactcggag tctgaagtcg ggaccaccgg aggccgggga 1261 ctgcctgtcc gcgccccgct cagcaccctg aacggcgaga tcagtgcctt ggcggccgag 1321 gcggcatgtg ttccagccga cgatcgcatc ttgtgtcgct gagaattcct ctagggcggc 1381 caattccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa 1441 ggccggtgtg cgtttgtcta tatgtgattt tccaccatat tgccgtcttt tggcaatgtg 1501 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc 1561 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct 1621 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac 1681 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc 1741 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta 1801 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg 1861 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg 1921 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccggga 1981 taattcctgc agccaatatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca 2041 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg 2101 agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc 2161 ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct 2221 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 2281 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt 2341 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 2401 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg 2461 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg 2521 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 2581 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga 2641 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcATGG 2701 ACGAGCTGTA CAAGTAAtct agagggccct attctatagt gtcacctaaa tgctagagct 2761 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc 2821 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 2881 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 2941 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg 3001 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc 3061 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 3121 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 3181 ccccgtcaag ctctaaatcg gggcatccct ttagggttcc gatttagtgc tttacggcac 3241 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 3301 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 3361 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgggg 3421 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc 3481 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt 3541 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 3601 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 3661 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 3721 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 3781 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 3841 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 3901 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 3961 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 4021 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg 4081 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 4141 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 4201 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 4261 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 4321 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 4381 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc 4441 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 4501 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 4561 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 4621 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 4681 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 4741 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 4801 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt 4861 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 4921 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 4981 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 5041 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 5101 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 5161 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 5221 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 5281 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 5341 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 5401 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 5461 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 5521 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 5581 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc 5641 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 5701 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 5761 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 5821 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 5881 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 5941 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 6001 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 6061 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 6121 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 6181 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 6241 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 6301 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 6361 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 6421 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 6481 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 6541 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 6601 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 6661 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 6721 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 6781 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 6841 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 6901 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 6961 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 7021 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 7081 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 7141 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c //