LOCUS pGfa2_myrTdTomat 6893 bp DNA circular 02-JUN-2020 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6893) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, Jun 2, 2020 from SnapGene Viewer 5.1.3 https://www.snapgene.com COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers protein_bind 106..127 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presenceof cAMP." /locus_tag="CAP binding site" /label="CAP binding site" /ApEinfo_label="CAP binding site" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" promoter 142..172 /note="promoter for the E. coli lac operon" /locus_tag="lac promoter" /label="lac promoter" /ApEinfo_label="lac promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" protein_bind 180..196 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /locus_tag="lac operator" /label="lac operator" /ApEinfo_label="lac operator" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" primer_bind 204..220 /note="common sequencing primer, one of multiple similar variants" /locus_tag="M13 rev" /label="M13 rev" /ApEinfo_label="M13 rev" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 253..2462 /note="pGfa2" /locus_tag="pGfa2" /label="pGfa2" /ApEinfo_label="pGfa2" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 2416..2429 /locus_tag="hGFAP 5' UTR" /label="hGFAP 5' UTR" /ApEinfo_label="hGFAP 5' UTR" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 2430..2432 /locus_tag="Mutated start codon" /label="Mutated start codon" /ApEinfo_label="Mutated start codon" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 2474..3928 /note="mTdTomato" /locus_tag="myrTdTomato" /label="myrTdTomato" /ApEinfo_label="myrTdTomato" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 2474..2476 /locus_tag="Start codon" /label="Start codon" /ApEinfo_label="Start codon" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" CDS 2498..3928 /codon_start=1 /product="tandem dimeric (pseudo-monomeric) derivative ofDsRed (Shaner et al., 2004)" /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQT A KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDG G LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEI H QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGR H HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRP Y EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERV M NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDG V LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQY E RSEGRHHLFLYGMDELYK" /locus_tag="tdTomato" /label="tdTomato" /ApEinfo_label="tdTomato" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 3944..4472 /note="mP-1" /locus_tag="mP-1" /label="mP-1" /ApEinfo_label="mP-1" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" primer_bind complement(4497..4513) /note="common sequencing primer, one of multiple similar variants" /locus_tag="M13 fwd" /label="M13 fwd" /ApEinfo_label="M13 fwd" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" promoter 4987..5091 /gene="bla" /locus_tag="AmpR promoter" /label="AmpR promoter" /ApEinfo_label="AmpR promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" CDS 5092..5952 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="confers resistance to ampicillin, carbenicillin,and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY I ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY S PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR W EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS A LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA S LIKHW" /locus_tag="AmpR" /label="AmpR" /ApEinfo_label="AmpR" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" rep_origin 6123..6711 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /locus_tag="ori" /label="ori" /ApEinfo_label="ori" /ApEinfo_fwdcolor="#999999" /ApEinfo_revcolor="#999999" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" ORIGIN 1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 181 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attcgagctc 241 ggtaccagat ctgagctccc acctccctct ctgtgctggg actcacagag ggagacctca 301 ggaggcagtc tgtccatcac atgtccaaat gcagagcata ccctgggctg ggcgcagtgg 361 cgcacaactg taattccagc actttgggag gctgatgtgg aaggatcact tgagcccaga 421 agttctagac cagcctgggc aacatggcaa gaccctatct ctacaaaaaa agttaaaaaa 481 tcagccacgt gtggtgacac acacctgtag tcccagctat tcaggaggct gaggtgaggg 541 gatcacttaa ggctgggagg ttgaggctgc agtgagtcgt ggttgcgcca ctgcactcca 601 gcctgggcaa cagtgagacc ctgtctcaaa agacaaaaaa aaaaaaaaaa aaaaaaagaa 661 catatcctgg tgtggagtag gggacgctgc tctgacagag gctcgggggc ctgagctggc 721 tctgtgagct ggggaggagg cagacagcca ggccttgtct gcaagcagac ctggcagcat 781 tgggctggcc gccccccagg gcctcctctt catgcccagt gaatgactca ccttggcaca 841 gacacaatgt tcggggtggg cacagtgcct gcttcccgcc gcaccccagc ccccctcaaa 901 tgccttccga gaagcccatt gagcaggggg cttgcattgc accccagcct gacagcctgg 961 catcttggga taaaagcagc acagccccct aggggctgcc cttgctgtgt ggcgccaccg 1021 gcggtggaga acaaggctct attcagcctg tgcccaggaa aggggatcag gggatgccca 1081 ggcatggaca gtgggtggca gggggggaga ggagggctgt ctgcttccca gaagtccaag 1141 gacacaaatg ggtgagggga ctgggcaggg ttctgaccct gtgggaccag agtggagggc 1201 gtagatggac ctgaagtctc cagggacaac agggcccagg tctcaggctc ctagttgggc 1261 ccagtggctc cagcgtttcc aaacccatcc atccccagag gttcttccca tctctccagg 1321 ctgatgtgtg ggaactcgag gaaataaatc tccagtggga gacggagggg tggccaggga 1381 aacggggcgc tgcaggaata aagacgagcc agcacagcca gctcatgtgt aacggctttg 1441 tggagctgtc aaggcctggt ctctgggaga gaggcacagg gaggccagac aaggaagggg 1501 tgacctggag ggacagatcc aggggctaaa gtcctgataa ggcaagagag tgccggcccc 1561 ctcttgccct atcaggacct ccactgccac atagaggcca tgattgaccc ttagacaaag 1621 ggctggtgtc caatcccagc ccccagcccc agaactccag ggaatgaatg ggcagagagc 1681 aggaatgtgg gacatctgtg ttcaagggaa ggactccagg agtctgctgg gaatgaggcc 1741 tagtaggaaa tgaggtggcc cttgagggta cagaacaggt tcattcttcg ccaaattccc 1801 agcaccttgc aggcacttac agctgagtga gataatgcct gggttatgaa atcaaaaagt 1861 tggaaagcag gtcagaggtc atctggtaca gcccttcctt cccttttttt tttttttttt 1921 ttgtgagaca aggtctctct ctgttgccca ggctggagtg gcgcaaacac agctcactgc 1981 agcctcaacc tactgggctc aagcaatcct ccagcctcag cctcccaaag tgctgggatt 2041 acaagcatga gccaccccac tcagcccttt ccttcctttt taattgatgc ataataattg 2101 taagtattca tcatggtcca accaaccctt tcttgaccca ccttcctaga gagagggtcc 2161 tcttgcttca gcggtcaggg ccccagaccc atggtctggc tccaggtacc acctgcctca 2221 tgcaggagtt ggcgtgccca ggaagctctg cctctgggca cagtgacctc agtggggtga 2281 ggggagctct ccccatagct gggctgcggc ccaaccccac cccctcaggc tatgccaggg 2341 ggtgttgcca ggggcacccg ggcatcgcca gtctagccca ctccttcata aagccctcgc 2401 atcccaggag cgagcagagc cagagcaggt tggagaggag acgcatcacc tccgctgctc 2461 gcggggatcc accatgggtt gctgtttctc caagaccatg gtgagcaagg gagaggaggt 2521 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt 2581 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa 2641 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta 2701 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt 2761 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt 2821 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac 2881 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac 2941 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct 3001 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt 3061 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga 3121 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctggggca 3181 tggcaccggc agcaccggca gcggcagctc cggcaccgcc tcctccgagg acaacaacat 3241 ggccgtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca 3301 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa 3361 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt 3421 catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct 3481 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt 3541 gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg 3601 cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc 3661 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct 3721 gaagctgaag gacggcggcc gctacctggt ggagttcaag accatctaca tggccaagaa 3781 gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa 3841 cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct 3901 gtacggcatg gacgagctgt acaagtaagg atccgcagat cccggccaga taccgatgct 3961 gccgcagcaa aagcaggagc agatgccgcc gtcgcaggcg aagatgtcgc agacggagga 4021 ggcgatgctg ccggcggagg aggcgaagta agtagagggc tgggctgggc tgtggggggt 4081 gtggggtgcg ggactgggca gtctgggagt ccctctcacc acttttctta cctttctagg 4141 atgctgccgt cgccgccgct catacaccat aaggtgtaaa aaatactaga tgcacagaat 4201 agcaagtcca tcaaaactcc tgcgtgagaa ttttaccaga cttcaagagc atctcgccac 4261 atcttgaaaa atgccaccgt ccgatgaaaa acaggagcct gctaaggaac aatgccacct 4321 gtcaataaat gttgaaaact catcccattc ctgcctcttg gtccttgggc ttggggaggg 4381 gtgcgcggat gtggttaggg aacatgactg gtcaaatggg aagggcttca aaagaattcc 4441 caatattgac taccaagcca cctgtacaga tctgcctgca ggcatgcaag cttggcactg 4501 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 4561 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 4621 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg 4681 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc 4741 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 4801 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 4861 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt 4921 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga 4981 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 5041 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 5101 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 5161 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 5221 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 5281 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 5341 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 5401 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 5461 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 5521 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 5581 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 5641 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 5701 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 5761 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 5821 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 5881 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 5941 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 6001 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 6061 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 6121 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 6181 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 6241 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac 6301 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 6361 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 6421 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 6481 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 6541 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 6601 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 6661 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 6721 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 6781 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 6841 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga aga //