LOCUS       Exported                5722 bp ds-DNA     circular SYN 01-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Hammond Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 16, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..589
                     /label=CMV
                     /note="CMV"
     misc_feature    1
                     /label=Kanamycin
                     /note="Kanamycin"
     misc_feature    1
                     /label=GFP tag
                     /note="GFP tag"
     primer_bind     519..539
                     /label=CMV-F
     CDS             613..1320
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     misc_feature    613..1320
                     /label=mNG
     misc_feature    613..633
                     /label=EGFP
                     /note="EGFP"
     CDS             613..633
                     /codon_start=1
                     /label=EGFP
                     /note="EGFP"
                     /translation="MVSKGEE"
     primer_bind     613..630
                     /label=FPi-F
                     /note="Amplify fluorescent protein from eGFP/CFP etc pr 
                     mCherry etc"
     primer_bind     616..633
                     /label=FPi-F2.0
     primer_bind     complement(616..633)
                     /label=FPv-R
                     /note="Amplify vector without eGFP/CFP etc pr mCherry etc. 
                     Use to exchange with FPi primers."
     regulatory      868..877
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     1202..1222
                     /label=NeonGFWD1
     primer_bind     complement(1301..1335)
                     /label=NG-NIR1-VR2
     misc_feature    1301..1320
                     /label=EGFP
                     /note="EGFP"
     CDS             1301..1320
                     /codon_start=1
                     /label=EGFP
                     /note="EGFP"
                     /translation="MDELYK"
     primer_bind     1301..1320
                     /label=FPv-F
                     /note="Amplify vector without eGFP/CFP etc pr mCherry etc. 
                     Use to exchange with FPi primers."
     primer_bind     complement(1301..1320)
                     /label=FPi-R
                     /note="Amplify fluorescent protein from eGFP/CFP etc pr 
                     mCherry etc"
     misc_feature    1321..1335
                     /label=Linker
     CDS             1339..2301
                     /codon_start=1
                     /label=Nir2-aLNS
                     /translation="SPAFPREKWQRKRTQVKIRNVTSNHRASDTVVCEGRPQVLSGRFM
                     YGPLDVVTLTGEKVDVYIMTQPLSGKWIHFGTEVTNSSGRLTFPVPPERALGIGVYPVR
                     MVVRGDHTYAECCLTVVARGTEAVVFSIDGSFTASVSIMGSDPKVRAGAVDVVRHWQDS
                     GYLIVYVTGRPDMQKHRVVAWLSQHNFPHGVVSFCDGLTHDPLRQKAMFLQSLVQEVEL
                     NIVAGYGSPKDVAVYAALGLSPSQTYIVGRAVRKLQAQCQFLSDGYVAHLGQLEAGSHS
                     HASSGPPRAALGKSSYGVAAPVDFLRKQSQLLRSRGPSQA"
     primer_bind     complement(2306..2330)
                     /label=NG-NIR1-IR2
     primer_bind     complement(2309..2320)
                     /label=NG-NIR1-IR
     primer_bind     2311..2339
                     /label=NG-NIR1-VF2
     misc_feature    2321..2408
                     /label=MCS
                     /note="MCS"
     primer_bind     2321..2340
                     /label=RepGFPf
     primer_bind     complement(2345..2365)
                     /label=INPPL1_V_R
     primer_bind     complement(2355..2374)
                     /label=EGFP_PIP5K1B_vR
     primer_bind     2367..2381
                     /label=INPPL1_V_F
     primer_bind     2379..2399
                     /label=EGFP_PIP5K1B_vF
     primer_bind     complement(2416..2435)
                     /label=SV40-pArev
     primer_bind     2469..2491
                     /label=A42
     misc_feature    2638..3093
                     /label=f1
                     /note="f1"
     promoter        3155..3183
                     /label=Bacterial
                     /note="Bacterial"
     promoter        3267..3496
                     /label=SV40early
                     /note="SV40early"
     misc_feature    3434..3569
                     /label=SV40
                     /note="SV40"
     misc_feature    3618..4412
                     /label=Kan/Neo
                     /note="Kan/Neo"
     misc_feature    4648..4666
                     /label=HSV-TK
                     /note="HSV-TK"
     misc_feature    4997..5640
                     /label=pUC
                     /note="pUC"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggcctctct cccagcgaca
      661 catgagttac acatctttgg ctccatcaac ggtgtggact ttgacatggt gggtcagggc
      721 accggcaatc caaatgatgg ttatgaggag ttaaacctga agtccaccaa gggtgacctc
      781 cagttctccc cctggattct ggtccctcat atcgggtatg gcttccatca gtacctgccc
      841 taccctgacg ggatgtcgcc tttccaggcc gccatggtag atggctccgg ataccaagtc
      901 catcgcacaa tgcagtttga agatggtgcc tcccttactg ttaactaccg ctacacctac
      961 gagggaagcc acatcaaagg agaggcccag gtgaagggga ctggtttccc tgctgacggt
     1021 cctgtgatga ccaactcgct gaccgctgcg gactggtgca ggtcgaagaa gacttacccc
     1081 aacgacaaaa ccatcatcag tacctttaag tggagttaca ccactggaaa tggcaagcgc
     1141 taccggagca ctgcgcggac cacctacacc tttgccaagc caatggcggc taactatctg
     1201 aagaaccagc cgatgtacgt gttccgtaag acggagctca agcactccaa gaccgagctc
     1261 aacttcaagg agtggcaaaa ggcctttacc gatgtgatgg gcatggacga gctgtacaag
     1321 ggaggctcag gaggcatgtc tccagcattt cctcgggaaa agtggcagcg taagcgcaca
     1381 caagtgaaga ttagaaacgt tacatcgaat caccgtgcgt cggataccgt agtgtgcgag
     1441 gggcgaccgc aggtgcttag tgggcggttt atgtacggcc ccttagatgt cgtaactttg
     1501 actggggaaa aggtggacgt gtacatcatg actcagccct tatcaggtaa atggatccac
     1561 tttggtactg aggtgacgaa cagcagtggt cgcctcactt tccctgttcc gccagagagg
     1621 gcattgggga ttggtgtcta cccagtgcgg atggtcgtaa gaggggacca tacgtatgcg
     1681 gagtgctgtc taacggtcgt cgcgcgcggc acagaggcag tggtattttc aatagacggc
     1741 tcattcactg ctagtgtttc cattatgggg tccgatccga aggttcgagc gggggcggtc
     1801 gatgtggtca ggcactggca agatagtggg tatctgatag tatatgtgac cgggcgcccc
     1861 gatatgcaaa aacatagagt agtcgcatgg ctttctcagc ataattttcc ccacggtgta
     1921 gtatcgtttt gcgacggtct cacacatgat ccactgagac aaaaagcaat gtttctacag
     1981 tccctggtcc aagaggtcga gctaaacatt gtagcaggat atggaagccc taaagacgtg
     2041 gcggtctatg cggccttagg gctatcgcca agtcagactt atatagttgg acgtgccgtc
     2101 agaaaacttc aggctcaatg ccagtttcta agtgatggtt acgtcgcaca tctggggcaa
     2161 cttgaagcgg gttcacactc tcatgcgagc tccggtccac ctagagctgc gctaggcaag
     2221 tcgtcttacg gcgtcgcagc gccagttgac ttcctacgaa agcagtcgca actcctccgg
     2281 agtcggggtc ctagtcaagc ataagcggcc gctcgagtct tccggactca gatctcgagc
     2341 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata
     2401 actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     2461 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     2521 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     2581 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa
     2641 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     2701 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     2761 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     2821 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     2881 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     2941 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     3001 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     3061 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     3121 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3181 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
     3241 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     3301 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     3361 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     3421 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3481 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     3541 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     3601 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     3661 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     3721 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3781 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3841 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3901 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3961 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     4021 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     4081 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     4141 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     4201 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     4261 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     4321 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     4381 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     4441 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     4501 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     4561 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     4621 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     4681 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     4741 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     4801 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
     4861 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4921 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4981 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5041 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5101 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5161 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5221 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5281 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5341 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5401 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5461 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5521 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5581 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5641 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5701 taaccgtatt accgccatgc at
//
