LOCUS       3._AAV-MYL2-SauriABE8e- 8021 bp ds-DNA     circular     19-FEB-2024
DEFINITION  AAV genome encoding SauriABE8e and Sa sgRNA cassette on a single
            AAV, with BsmBI sites for protospacer installation.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name:
            AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI.gbk
FEATURES             Location/Qualifiers
     repeat_region   22..151
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    217..551
                     /label="MYL2 promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    565..4464
                     /label="start codon-BPNLS-TadA-SauriCas9 D10A-BPNLS-stop codon"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             565..4464
                     /label="Translation 565-4464"
                     /translation="MKRTADGSEFESPKKKRKVSEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFGVRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQSSINSGGSSGGSSGSETPGTSESATPESSGGSSGGSMQENQQKQNYILGLAIGITSVGYGLIDSKTREVIDAGVRLFPEADSENNSNRRSKRGARRLKRRRIHRLNRVKDLLADYQMIDLNNVPKSTDPYTIRVKGLREPLTKEEFAIALLHIAKRRGLHNISVSMGDEEQDNELSTKQQLQKNAQQLQDKYVCELQLERLTNINKVRGEKNRFKTEDFVKEVKQLCETQRQYHNIDDQFIQQYIDLVSTRREYFEGPGNGSPYGWDGDLLKWYEKLMGRCTYFPEELRSVKYAYSADLFNALNDLNNLVVTRDDNPKLEYYEKYHIIENVFKQKKNPTLKQIAKEIGVQDYDIRGYRITKSGKPQFTSFKLYHDLKNIFEQAKYLEDVEMLDEIAKILTIYQDEISIKKALDQLPELLTESEKSQIAQLTGYTGTHRLSLKCIHIVIDELWESPENQMEIFTRLNLKPKKVEMSEIDSIPTTLVDEFILSPVVKRAFIQSIKVINAVINRFGLPEDIIIELAREKNSKDRRKFINKLQKQNEATRKKIEQLLAKYGNTNAKYMIEKIKLHDMQEGKCLYSLEAIPLEDLLSNPTHYEVDHIIPRSVSFDNSLNNKVLVKQSENSKKGNRTPYQYLSSNESKISYNQFKQHILNLSKAKDRISKKKRDMLLEERDINKFEVQKEFINRNLVDTRYATRELSNLLKTYFSTHDYAVKVKTINGGFTNHLRKVWDFKKHRNHGYKHHAEDALVIANADFLFKTHKALRRTDKILEQPGLEVNDTTVKVDTEEKYQELFETPKQVKNIKQFRDFKYSHRVDKKPNRQLINDTLYSTREIDGETYVVQTLKDLYAKDNEKVKKLFTERPQKILMYQHDPKTFEKLMTILNQYAEAKNPLAAYYEDKGEYVTKYAKKGNGPAIHKIKYIDKKLGSYLDVSNKYPETQNKLVKLSLKSFRFDIYKCEQGYKMVSIGYLDVLKKDNYYYIPKDKYEAEKQKKKIKESDLFVGSFYYNDLIMYEDELFRVIGVNSDINNLVELNMVDITYKDFCEVNNVTGEKRIKKTIGKRVVLIEKYTTDILGNLYKTPLPKKPQLIFKRGELSGGSKRTADGSEFEPKKKRKV*"
     CDS             601..621
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /product="nuclear localization signal of SV40 (simian virus40) large T antigen"
                     /translation="PKKKRKV"
     misc_feature    622..1119
                     /label="ABE8e"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(769..783)
                     /label="seq-Sauri-2-R"
                     /note="sequence: tgttgggtcgtgcag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          846..867
                     /label="ABE8e-QP-F"
                     /note="sequence: tgacgccaccctgtacgtgaca"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(972..993)
                     /label="ABE8e-QP-R"
                     /note="sequence: gccggggtagttcagcacgttc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1218..1237
                     /label="seq-Sauri-1"
                     /note="sequence: gcaggagaaccagcagaagc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(1364..1383)
                     /label="seq-Sauri-1-R"
                     /note="sequence: ccgcttagatcttctattgc"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1654..1675
                     /label="SauriCas9-QP-F"
                     /note="sequence: aagaacgctcagcagctgcagg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(1791..1812)
                     /label="SauriCas9-QP-R"
                     /note="sequence: gttgtggtactgccgctgggtc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2019..2038
                     /label="seq-Sauri-2"
                     /note="sequence: cgatctgaacaacctggttg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2799..2818
                     /label="seq-sauri-3"
                     /note="sequence: gatcgaaaagatcaagctgc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          3575..3594
                     /label="seq-sauri-4"
                     /note="sequence: ggcagctgatcaatgatacc"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          4357..4376
                     /label="seq-sauri-5"
                     /note="sequence: ctgcctaagaagccccagct"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4399..4464
                     /label="SaKKH-Sauri-Nme2-Cj-overlap2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             4441..4461
                     /label="Translation 4441-4461"
                     /translation="PKKKRKV"
     primer          4441..4461
                     /label="NLS-F"
                     /note="sequence: cccaagaagaagaggaaagtc"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     polyA_signal    4475..4699
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    4718..4808
                     /label="Scaffold in vivo"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(4745..4764)
                     /label="Scaffold-R"
                     /note="sequence: ggcaaaatgccgtgtttatc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(4809..4829)
                     /label="sgRNA"
                     /note="sequence: ctacttAccaaaccatgcctg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     promoter        complement(4839..5079)
                     /label="U6 promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     complement(4889..4908)
                     /label="LKO.1 5'"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(5059..5079)
                     /label="hU6-F"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="Human U6 promoter, forward primer"
     repeat_region   5103..5232
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(5251..5267)
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="common sequencing primer, one of multiple similar variants"
     primer_bind     complement(5251..5268)
                     /label="M13 Forward"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward"
     primer_bind     complement(5260..5282)
                     /label="M13/pUC Forward"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="In lacZ gene"
     rep_origin      5409..5864
                     /label="f1 ori"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
                     /direction="RIGHT"
     primer_bind     complement(5496..5515)
                     /label="F1ori-R"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="F1 origin, reverse primer"
     primer_bind     5706..5727
                     /label="F1ori-F"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="F1 origin, forward primer"
     promoter        5890..5994
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /gene="bla"
     CDS             5995..6855
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"
                     /gene="bla"
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     primer_bind     complement(6213..6232)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7026..7614
                     /label="ori"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /direction="RIGHT"
     primer_bind     7515..7534
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="pBR322 origin, forward primer"
     primer_bind     7768..7785
                     /label="L4440"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="L4440 vector, forward primer"
     protein_bind    7902..7923
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="CAP binding activates transcription in the presence of cAMP."
                     /bound_moiety="E. coli catabolite activator protein"
     promoter        7938..7968
                     /label="lac promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7976..7992
                     /label="lac operator"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /bound_moiety="lac repressor encoded by lacI"
     primer_bind     7981..8003
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="In lacZ gene"
     primer_bind     8000..8016
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8000..8016
                     /label="M13 rev"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="common sequencing primer, one of multiple similar variants"
ORIGIN
        1 ttacgccaga tttaattaag gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
       61 cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag
      121 ggagtggcca actccatcac taggggttcc tgcggcctct agaattcgct agctaggtct
      181 tgaaaggagt gggaattggc tccggtgccc gtcagtgggt tactcttccc cattagacaa
      241 tggcagggaa gagagcacac cccatcatcc ccaggccagg ccccagccac tgactcttta
      301 accttgaagg catttttggg tctcacgtgt ccacccaggc gggtggccgc ctttgagcag
      361 ctcttacttc agaagaacgg catggagtgg ggggtggggg gcttaggtgg cctccgcctc
      421 acctacaact gccaaaagtg gtcatggggt tatttttaac cccaggggag aggtatttat
      481 tgttccacag caggggcaga ggccagcagg ctcctcgaac tctccagagg tggcaactgg
      541 cctcagacac cgaccggtgc caccatgaaa cggacagccg acggaagcga gttcgagtca
      601 ccaaagaaga agcggaaagt ctctgaggtg gagttttccc acgagtactg gatgagacat
      661 gccctgaccc tggccaagag ggcacgggat gagagggagg tgcctgtggg agccgtgctg
      721 gtgctgaaca atagagtgat cggcgagggc tggaacagag ccatcggcct gcacgaccca
      781 acagcccatg ccgaaattat ggccctgaga cagggcggcc tggtcatgca gaactacaga
      841 ctgattgacg ccaccctgta cgtgacattc gagccttgcg tgatgtgcgc cggcgccatg
      901 atccactcta ggatcggccg cgtggtgttt ggcgtgagga actcaaaaag aggcgccgca
      961 ggctccctga tgaacgtgct gaactacccc ggcatgaatc accgcgtcga aattaccgag
     1021 ggaatcctgg cagatgaatg tgccgccctg ctgtgcgatt tctatcggat gcctagacag
     1081 gtgttcaatg ctcagaagaa ggcccagagc tccatcaact ccggaggatc tagcggaggc
     1141 tcctctggct ctgagacacc tggcacaagc gagagcgcaa cacctgaaag cagcgggggc
     1201 agcagcgggg ggtcaatgca ggagaaccag cagaagcaaa attacatcct gggcctggcc
     1261 atcggcatca ccagcgtggg ctatggcctg atcgacagca agaccagaga agtgattgac
     1321 gccggcgtgc ggctattccc agaggccgac tctgaaaaca acagcaatag aagatctaag
     1381 cggggcgccc ggagactgaa aagacgaaga atccacagac tgaacagagt gaaagacctc
     1441 ctggctgact accagatgat cgacttaaac aacgtgccca agtctaccga cccctacacc
     1501 atccgggtga agggactgcg ggaacctctg accaaggaag agtttgccat cgctctgctg
     1561 catatcgcca agagaagagg cctgcacaac atctccgtga gcatgggcga tgaggaacag
     1621 gacaacgagc tgtccaccaa gcagcagctg cagaagaacg ctcagcagct gcaggacaaa
     1681 tacgtgtgcg agctgcaact ggaaagactg accaacatca acaaggtcag aggcgagaag
     1741 aaccggttca agaccgaaga tttcgtgaag gaagtgaagc agctgtgcga gacccagcgg
     1801 cagtaccaca acatcgacga tcagttcatc cagcagtaca tcgacctggt gagcacccgg
     1861 agagaatact tcgagggccc tggcaacggc tctccatatg gctgggatgg agatctgctg
     1921 aagtggtatg agaagctgat gggcagatgc acctacttcc ctgaggagct gagaagcgtg
     1981 aagtacgcct acagcgccga tctgtttaac gccctgaacg atctgaacaa cctggttgtg
     2041 acccgcgacg acaaccctaa gctggaatac tacgagaaat accacattat cgagaacgtg
     2101 ttcaagcaga agaaaaatcc aactctgaag caaatcgcca aagagatcgg cgtgcaggat
     2161 tacgacatca gaggatacag aattaccaag tccggtaagc ctcagttcac cagcttcaaa
     2221 ctctaccacg acctgaaaaa catctttgaa caggccaaat acctggaaga tgtggagatg
     2281 ctggatgaga tagctaaaat cctgacaatc taccaagacg agatcagcat caagaaagcc
     2341 ctggaccagc tgcctgagct gctgaccgag agcgaaaaaa gccagatcgc tcagctgacc
     2401 ggctacaccg gtacacatag actgtctctg aagtgcatcc acatcgtgat cgacgagctg
     2461 tgggagagcc ccgaaaacca gatggaaatc ttcaccagac tgaacctcaa gccaaagaag
     2521 gtggaaatga gcgagatcga cagcatccct accacactgg tggatgagtt catcctgagc
     2581 cctgtggtga agcgggcctt catccagtcc atcaaggtga tcaacgctgt gatcaacaga
     2641 ttcggcctgc ccgaggacat cattatcgag ctggccagag agaagaacag caaggacaga
     2701 aggaagttca tcaacaagct gcagaaacag aatgaggcca cccggaaaaa aatcgagcag
     2761 ctgctggcca agtacggcaa taccaatgcc aagtacatga tcgaaaagat caagctgcat
     2821 gacatgcagg agggcaagtg tctgtacagc ctggaagcta tccccctgga agacctgctg
     2881 tctaatccta cacactacga ggtggaccac atcatcccta gaagcgtgtc cttcgacaac
     2941 agcctgaaca acaaggttct ggtgaagcaa agcgagaaca gcaagaaggg caataggacc
     3001 ccttaccagt acctgagcag caacgagtcc aagatctctt acaaccagtt caagcagcac
     3061 attctgaacc tgtctaaggc caaagataga atcagcaaga agaaacgaga tatgctgctg
     3121 gaagaacggg acatcaacaa attcgaggtg cagaaggaat tcatcaacag aaaccttgtg
     3181 gacacccggt acgccactcg ggagctgagc aacctgctga agacctactt cagcacacac
     3241 gactacgccg tgaaagtgaa gaccatcaac ggcggcttca caaaccacct gaggaaggtg
     3301 tgggacttca agaagcaccg gaaccacggc tacaagcacc acgccgagga tgccctggtc
     3361 atcgccaacg ccgactttct gttcaaaacc cacaaggccc tgagacggac agacaagatc
     3421 ctggaacagc ctggactgga agtcaacgac accaccgtga aggtggacac agaggagaag
     3481 taccaggagt tattcgagac accgaaacaa gtgaagaaca tcaagcagtt tagagatttc
     3541 aagtattctc acagagttga caagaagccc aaccggcagc tgatcaatga taccctgtac
     3601 agtaccagag agatcgatgg cgaaacctac gtggtccaaa cactgaaaga cctgtacgcc
     3661 aaggacaatg aaaaggtgaa aaagctcttt acagaacggc ctcaaaagat actgatgtac
     3721 cagcacgatc ctaagacctt tgagaaactg atgaccattc tgaatcagta cgctgaggca
     3781 aagaatcctc tggccgctta ttacgaggat aagggcgaat acgtgaccaa gtacgccaag
     3841 aagggcaacg gccctgccat ccacaagatc aaatacatcg acaagaaact gggcagctac
     3901 ctggacgtga gtaacaaata tcctgagaca cagaacaagc tggtgaaact gtctctgaag
     3961 agctttagat tcgacatcta caaatgtgaa cagggctaca agatggtgtc cattggctac
     4021 ctcgacgtac tgaagaagga caactactac tacatcccca aagataagta cgaggccgag
     4081 aagcagaaaa agaagatcaa ggaaagcgac ctcttcgtgg gcagcttcta ctacaacgac
     4141 ctgatcatgt acgaggacga actcttccgg gtgatcggag tgaactccga tatcaacaac
     4201 ctggttgagc tgaatatggt cgacatcacc tacaaggatt tctgcgaggt gaacaacgtg
     4261 acaggcgaga agagaatcaa gaaaaccatc ggaaagagag tggtgctgat cgagaagtat
     4321 acgaccgaca tcctgggaaa tctgtataaa acgcccctgc ctaagaagcc ccagctcatt
     4381 ttcaagagag gcgagctgtc tggcggctca aaaagaaccg ccgacggcag cgaattcgag
     4441 cccaagaaga agaggaaagt ctaatagatc tcgactgtgc cttctagttg ccagccatct
     4501 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     4561 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     4621 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     4681 gatgcggtgg gctctatggc tcgagcggcc caagcttaaa aaaatctcgc caacaagttg
     4741 acgagataaa cacggcattt tgccttgttt tagtagattc tgtaattttc attacagagt
     4801 actaaaacca ggcatggttt ggTaagtagc ggtgtttcgt cctttccaca agatatataa
     4861 agccaagaaa tcgaaatact ttcaagttac ggtaagcata tgatagtcca ttttaaaaca
     4921 taattttaaa actgcaaact acccaagaaa ttattacttt ctacgtcacg tattttgtac
     4981 taatatcttt gtgtttacag tcaaattaat tctaattatc tctctaacag ccttgtatcg
     5041 tatatgcaaa tatgaaggaa tcatgggaaa taggccctct tcctgcccga ccttgcggcc
     5101 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     5161 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     5221 gcgagcgcgc agccttaatt aacctaattc actggccgtc gttttacaac gtcgtgactg
     5281 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     5341 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     5401 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     5461 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     5521 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     5581 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     5641 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     5701 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     5761 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     5821 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     5881 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5941 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6001 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6061 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6121 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6181 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     6241 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6301 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6361 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     6421 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     6481 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     6541 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     6601 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     6661 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     6721 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6781 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6841 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6901 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6961 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7021 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7081 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7141 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7201 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7261 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7321 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7381 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7441 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7501 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7561 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7621 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7681 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     7741 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     7801 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     7861 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7921 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7981 agcggataac aatttcacac aggaaacagc tatgaccatg a
//