LOCUS       9._pAAV-MYL2-HA-ABE8e-S 6147 bp ds-DNA     circular     25-FEB-2024
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name:
            addgene-plasmid-80930-sequence-152874.gbk Expresses Cas9N in
            mammalian cells..
FEATURES             Location/Qualifiers
     primer_bind     143..162
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     396..413
                     /label="L4440"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     protein_bind    530..551
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        566..596
                     /label="lac promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     protein_bind    604..620
                     /label="lac operator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     609..631
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     628..644
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     628..644
                     /label="M13 rev"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     repeat_region   671..800
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(848..868)
                     /label="pBABE 3'"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    896..915
                     /label="overlap"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    915..934
                     /label="overlap"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     enhancer        916..934
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          916..954
                     /label="SpeI-MYL2-F"
                     /note="sequence: gacattgattattgactaggggttactcttccccattag"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    935..1269
                     /label="MYL2"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1281..1305
                     /label="overlap"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          1281..1355
                     /label="NcoI-HA-F"
                     /note="sequence: gctagcgctaccggtcgccaccatggcctacccatacgatgttccagattacgctccaaagaagaagcggaaagt"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     regulatory      1297..1306
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1303..1308
                     /label="Translation 1303-1308"
                     /translation="MA"
     misc_feature    1309..1335
                     /label="HA"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1309..1335
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="YPYDVPDYA"
     misc_feature    1311..1334
                     /label="overlap"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1336..1356
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             1336..1950
                     /label="Translation 1336-1950"
                     /translation="PKKKRKVSEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFGVRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQSSINSGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     misc_feature    1357..1854
                     /label="ABE8e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1504..1518)
                     /label="seq-Sauri-2-R"
                     /note="sequence: tgttgggtcgtgcag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1581..1602
                     /label="ABE8e-QP-F"
                     /note="sequence: tgacgccaccctgtacgtgaca"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1827..1842
                     /label="overlap"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1827..1846
                     /label="SSNC-over1-F"
                     /note="sequence: tcagaagaaggcccagagct"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    1855..1950
                     /label="Linker"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1951..3264
                     /label="Sauri N aa1-438"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             1951..3264
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MQENQQKQNYILGLAIGITSVGYGLIDSKTREVIDAGVRLFPEADSENNSNRRSKRGARRLKRRRIHRLNRVKDLLADYQMIDLNNVPKSTDPYTIRVKGLREPLTKEEFAIALLHIAKRRGLHNISVSMGDEEQDNELSTKQQLQKNAQQLQDKYVCELQLERLTNINKVRGEKNRFKTEDFVKEVKQLCETQRQYHNIDDQFIQQYIDLVSTRREYFEGPGNGSPYGWDGDLLKWYEKLMGRCTYFPEELRSVKYAYSADLFNALNDLNNLVVTRDDNPKLEYYEKYHIIENVFKQKKNPTLKQIAKEIGVQDYDIRGYRITKSGKPQFTSFKLYHDLKNIFEQAKYLEDVEMLDEIAKILTIYQDEISIKKALDQLPELLTESEKSQIAQLTGYTGTHRLSLKCIHIVIDELWESPENQMEIFTRLNLKPKKVEM"
     primer          1953..1972
                     /label="seq-Sauri-1"
                     /note="sequence: gcaggagaaccagcagaagc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1993..1995
                     /label="D15A"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2099..2118)
                     /label="seq-Sauri-1-R"
                     /note="sequence: ccgcttagatcttctattgc"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2389..2410
                     /label="SauriCas9-QP-F"
                     /note="sequence: aagaacgctcagcagctgcagg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2526..2547)
                     /label="SauriCas9-QP-R"
                     /note="sequence: gttgtggtactgccgctgggtc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2647..2671
                     /label="overlap"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2647..2671)
                     /label="BglII-over-R"
                     /note="sequence: gcttctcataccacttcagcagatc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2754..2773
                     /label="seq-Sauri-2"
                     /note="sequence: cgatctgaacaacctggttg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          3121..3140
                     /label="Seq-SauriN-F"
                     /note="sequence: atcgctcagctgaccggcta"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(3243..3264)
                     /label="SauriN-over-R"
                     /note="sequence: catttccaccttctttggcttg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3247..3264
                     /label="overlap"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          3247..3284
                     /label="SauriN-over-F"
                     /note="sequence: ccaaagaaggtggaaatgtgtctggctggcgatactct"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3265..3570
                     /label="Rma Intein-N"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             3265..3570
                     /label="Translation 3265-3570"
                     /translation="CLAGDTLITLADGRRVPIRELVSQQNFSVWALNPQTYRLERARVSRAFCTGIKPVYRLTTRLGRSIRATANHRFLTPQGWKRVDELQPGDYLALPRRIPTAS"
     CDS             3571..3573
                     /label="Translation 3571-3573"
                     /translation="*"
     primer          complement(3574..3594)
                     /label="SacI-over-R"
                     /note="sequence: ccaaactcatcgagctcgaga"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    3579..3594
                     /label="overlap"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(3584..3603)
                     /label="EBV-rev"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     polyA_signal    3613..3694
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     3638..3657
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     repeat_region   3877..4006
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(4025..4041)
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(4025..4042)
                     /label="M13 Forward"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(4034..4056)
                     /label="M13/pUC Forward"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      4183..4638
                     /label="f1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     complement(4270..4289)
                     /label="F1ori-R"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     4480..4501
                     /label="F1ori-F"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        4665..4769
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             4770..5630
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     complement(4988..5007)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      5801..242
                     /label="ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
       61 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      121 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      181 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      241 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctgcggt tttgctcaca
      301 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      361 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      421 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      481 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      541 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      601 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccagat
      661 ttaattaagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg
      721 ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa
      781 ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact tatctacctg
      841 cggccgcgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
      901 agtatgcaaa gcatggacat tgattattga ctaggggtta ctcttcccca ttagacaatg
      961 gcagggaaga gagcacaccc catcatcccc aggccaggcc ccagccactg actctttaac
     1021 cttgaaggca tttttgggtc tcacgtgtcc acccaggcgg gtggccgcct ttgagcagct
     1081 cttacttcag aagaacggca tggagtgggg ggtggggggc ttaggtggcc tccgcctcac
     1141 ctacaactgc caaaagtggt catggggtta tttttaaccc caggggagag gtatttattg
     1201 ttccacagca ggggcagagg ccagcaggct cctcgaactc tccagaggtg gcaactggcc
     1261 tcagacaccg taccgaagcc gctagcgcta ccggtcgcca ccatggccta cccatacgat
     1321 gttccagatt acgctccaaa gaagaagcgg aaagtctctg aggtggagtt ttcccacgag
     1381 tactggatga gacatgccct gaccctggcc aagagggcac gggatgagag ggaggtgcct
     1441 gtgggagccg tgctggtgct gaacaataga gtgatcggcg agggctggaa cagagccatc
     1501 ggcctgcacg acccaacagc ccatgccgaa attatggccc tgagacaggg cggcctggtc
     1561 atgcagaact acagactgat tgacgccacc ctgtacgtga cattcgagcc ttgcgtgatg
     1621 tgcgccggcg ccatgatcca ctctaggatc ggccgcgtgg tgtttggcgt gaggaactca
     1681 aaaagaggcg ccgcaggctc cctgatgaac gtgctgaact accccggcat gaatcaccgc
     1741 gtcgaaatta ccgagggaat cctggcagat gaatgtgccg ccctgctgtg cgatttctat
     1801 cggatgccta gacaggtgtt caatgctcag aagaaggccc agagctccat caactccgga
     1861 ggatctagcg gaggctcctc tggctctgag acacctggca caagcgagag cgcaacacct
     1921 gaaagcagcg ggggcagcag cggggggtca atgcaggaga accagcagaa gcaaaattac
     1981 atcctgggcc tggccatcgg catcaccagc gtgggctatg gcctgatcga cagcaagacc
     2041 agagaagtga ttgacgccgg cgtgcggcta ttcccagagg ccgactctga aaacaacagc
     2101 aatagaagat ctaagcgggg cgcccggaga ctgaaaagac gaagaatcca cagactgaac
     2161 agagtgaaag acctcctggc tgactaccag atgatcgact taaacaacgt gcccaagtct
     2221 accgacccct acaccatccg ggtgaaggga ctgcgggaac ctctgaccaa ggaagagttt
     2281 gccatcgctc tgctgcatat cgccaagaga agaggcctgc acaacatctc cgtgagcatg
     2341 ggcgatgagg aacaggacaa cgagctgtcc accaagcagc agctgcagaa gaacgctcag
     2401 cagctgcagg acaaatacgt gtgcgagctg caactggaaa gactgaccaa catcaacaag
     2461 gtcagaggcg agaagaaccg gttcaagacc gaagatttcg tgaaggaagt gaagcagctg
     2521 tgcgagaccc agcggcagta ccacaacatc gacgatcagt tcatccagca gtacatcgac
     2581 ctggtgagca cccggagaga atacttcgag ggccctggca acggctctcc atatggctgg
     2641 gatggagatc tgctgaagtg gtatgagaag ctgatgggca gatgcaccta cttccctgag
     2701 gagctgagaa gcgtgaagta cgcctacagc gccgatctgt ttaacgccct gaacgatctg
     2761 aacaacctgg ttgtgacccg cgacgacaac cctaagctgg aatactacga gaaataccac
     2821 attatcgaga acgtgttcaa gcagaagaaa aatccaactc tgaagcaaat cgccaaagag
     2881 atcggcgtgc aggattacga catcagagga tacagaatta ccaagtccgg taagcctcag
     2941 ttcaccagct tcaaactcta ccacgacctg aaaaacatct ttgaacaggc caaatacctg
     3001 gaagatgtgg agatgctgga tgagatagct aaaatcctga caatctacca agacgagatc
     3061 agcatcaaga aagccctgga ccagctgcct gagctgctga ccgagagcga aaaaagccag
     3121 atcgctcagc tgaccggcta caccggtaca catagactgt ctctgaagtg catccacatc
     3181 gtgatcgacg agctgtggga gagccccgaa aaccagatgg aaatcttcac cagactgaac
     3241 ctcaagccaa agaaggtgga aatgtgtctg gctggcgata ctctcattac cctggccgat
     3301 ggacgacgag tgcctattag agaactggtg tcacagcaga atttttccgt gtgggctctg
     3361 aatcctcaga cttaccgcct ggagagggct agagtgagta gagctttctg taccggcatc
     3421 aaacctgtgt accgcctcac cactagactg gggagatcca ttagggccac tgccaaccac
     3481 cgatttctca cacctcaggg ctggaaacga gtcgatgaac tccagcctgg agattacctg
     3541 gctctgccta ggagaatccc tactgcctcc tgatctcgag ctcgatgagt ttggacaaac
     3601 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     3661 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     3721 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     3781 tggtagcggc cgcggaatgt gtgtcagtaa aacctctaca aatgtggtag cggccgcagg
     3841 tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg
     3901 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     3961 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagcctt aattaaccta
     4021 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     4081 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     4141 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     4201 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4261 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4321 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4381 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4441 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4501 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4561 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4621 tattaacgtt tataatttca ggtggcatct ttcggggaaa tgtgcgcgga acccctattt
     4681 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4741 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4801 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4861 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaata
     4921 gtggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4981 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5041 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5101 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5161 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5221 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5281 taccaaacga cgagcgtgac accacgatgc ctgtagtaat ggtaacaacg ttgcgcaaac
     5341 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5401 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5461 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5521 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5581 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5641 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5701 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5761 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5821 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5881 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5941 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6001 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6061 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6121 cggggggttc gtgcacacag cccagct
//