LOCUS       pGL_4.79-mCherry        4722 bp DNA     circular SYN 17-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 17, 2024 from SnapGene 7.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..120
                     /label=mCherryBglIIkozakstartFor
     regulatory      94..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             100..849
                     /codon_start=1
                     /label=mCherry
                     /translation="MGPGLGMLSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGE
                     GRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKW
                     ERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYP
                     EDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIV
                     EQYERAEGRHSTGGMDELYKGLEGGAP"
     primer_bind     complement(835..854)
                     /label=mCherry MFE1 STOP reverse primer
     polyA_signal    1010..1131
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1234..1591
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1442..1577
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1622..2416
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    2441..2489
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(2816..3404)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(3604..4395,4396..4464))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4569..4617
                     /note="synthetic polyadenylation signal"
     misc_feature    4631..4722
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4671..4690
                     /label=RV3 Primer
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tgggcccggg cctagggatg
      121 ttgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
      181 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      241 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
      301 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
      361 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
      421 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct acaggacggc
      481 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
      541 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
      601 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
      661 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
      721 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
      781 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggtct agaggggggc
      841 gcgccttaag aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata
      901 actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
      961 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     1021 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     1081 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaagccctta
     1141 ggggatcctt tgcgtattgg gcgctcttcc gctgatctgc gcagcaccat ggcctgaaat
     1201 aacctctgaa agaggaactt ggttagctac cttctgaggc ggaaagaacc agctgtggaa
     1261 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     1321 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     1381 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     1441 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     1501 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     1561 aggctttttt ggaggcctag gcttttgcaa aaagctcgat tcttctgaca ctagcgccac
     1621 catgatcgaa caagacggcc tccatgctgg cagtcccgca gcttgggtcg aacgcttgtt
     1681 cgggtacgac tgggcccagc agaccatcgg atgtagcgat gcggccgtgt tccgtctaag
     1741 cgctcaaggc cggcccgtgc tgttcgtgaa gaccgacctg agcggcgccc tgaacgagct
     1801 tcaagacgag gctgcccgcc tgagctggct ggccaccacc ggcgtaccct gcgccgctgt
     1861 gttggatgtt gtgaccgaag ccggccggga ctggctgctg ctgggcgagg tccctggcca
     1921 ggatctgctg agcagccacc ttgcccccgc tgagaaggtt tctatcatgg ccgatgcaat
     1981 gcggcgcctg cacaccctgg accccgctac ctgccccttc gaccaccagg ctaagcatcg
     2041 gatcgagcgt gctcggaccc gcatggaggc cggcctggtg gaccaggacg acctggacga
     2101 ggagcatcag ggcctggccc ccgctgaact gttcgcccga ctgaaagccc gcatgccgga
     2161 cggtgaggac ctggttgtca cacacggaga tgcctgcctc cctaacatca tggtcgagaa
     2221 tggccgcttc tccggcttca tcgactgcgg tcgcctagga gttgccgacc gctaccagga
     2281 catcgccctg gccacccgcg acatcgctga ggagcttggc ggcgagtggg ccgaccgctt
     2341 cttagtcttg tacggcatcg cagctcccga cagccagcgc atcgccttct accgcttgct
     2401 cgacgagttc ttttaatgat ctagaaccgg tcatggccgc aataaaatat ctttattttc
     2461 attacatctg tgtgttggtt ttttgtgtgt tcgaactaga tgctgtcgac cgatgccctt
     2521 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
     2581 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg
     2641 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2701 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2761 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2821 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2881 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2941 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3001 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3061 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3121 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3181 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3241 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3301 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3361 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3421 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3481 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3541 tctaaagtat atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag
     3601 tggttaccag tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc
     3661 catagtggcc tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg
     3721 ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat
     3781 gaaccagcca gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat
     3841 ccagtctatg agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg
     3901 aagagttgtg gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc
     3961 gttcaactct ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa
     4021 tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc
     4081 gctcatggta atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt
     4141 ttccgtgacc ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag
     4201 ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt
     4261 gctcatcatc gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag
     4321 atccagttcg atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac
     4381 cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc
     4441 gacacgaaaa tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca
     4501 gggttactag tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga
     4561 caggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
     4621 cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat
     4681 aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ct
//
