LOCUS       Exported               12180 bp ds-DNA     linear   UNA 06-MAY-2024
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 6, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12180
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          10779..11489
                     /organism="unspecified"
                     /mol_type="genomic DNA"
                     /label=source:unspecified
                     /note="/locus_tag=source:unspecified"
     source          11544..11562
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="/locus_tag=source:synthetic DNA construct"
     LTR             91..324
                     /locus_tag="3' LTR (Delta-U3)"
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR)
                     from HIV-1"
     promoter        complement(349..367)
                     /locus_tag="SP6 promoter"
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(350..367)
                     /locus_tag="SP6"
                     /label=SP6
     promoter        1155..1259
                     /gene="bla"
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
     CDS             1458..2117
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      2291..2879
                     /direction=RIGHT
                     /locus_tag="ori"
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3125..3454
                     /locus_tag="SV40 promoter"
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          4588..4653
                     /locus_tag="small t intron"
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4783..4803
                     /codon_start=1
                     /locus_tag="SV40 NLS"
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5228..5362
                     /locus_tag="SV40 poly(A) signal"
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             5531..6164
                     /locus_tag="3' LTR"
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6211..6336
                     /locus_tag="HIV-1 Psi"
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    6833..7065
                     /locus_tag="RRE"
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     repeat_region   7553..7732
                     /locus_tag="cPPT"
                     /label=cPPT
     primer_bind     7708..7731
                     /locus_tag="cPPT_5'seq"
                     /label=cPPT_5'seq
     misc_feature    complement(7770..7778)
                     /locus_tag="CTCF binding site"
                     /label=CTCF binding site
     misc_feature    7945..7958
                     /locus_tag="CTCF binding site(1)"
                     /label=CTCF binding site
                     /label=CTCF binding site(1)
     misc_feature    7997..8435
                     /locus_tag="UCOE"
                     /label=UCOE
     misc_feature    8368..8376
                     /locus_tag="missing in actual plasmid"
                     /label=missing in actual plasmid
     misc_feature    8435..8436
                     /locus_tag="origin"
                     /label=origin
     promoter        8437..9615
                     /locus_tag="EF-1-alpha promoter(1)"
                     /label=EF-1-alpha promoter
                     /label=EF-1-alpha promoter(1)
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     promoter        8437..8515
                     /locus_tag="EF-1-alpha promoter"
                     /label=EF-1-alpha promoter
     misc_feature    8437..8515
                     /locus_tag="EFS"
                     /label=EFS
     misc_feature    8437..8515
                     /locus_tag="origin(1)"
                     /label=origin
                     /label=origin(1)
     intron          8668..9606
                     /locus_tag="EF-1-alpha intron A"
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
     misc_feature    9664..9677
                     /locus_tag="HindIIIKpnI"
                     /label=HindIIIKpnI
     gene            9681..10376
                     /locus_tag="BFP"
                     /label=BFP
     misc_feature    10377..10442
                     /locus_tag="P2A"
                     /label=P2A
     misc_feature    10455..10640
                     /locus_tag="Sec61B-linker"
                     /label=Sec61B-linker
     misc_feature    10647..10661
                     /locus_tag="SEC61B flank"
                     /label=SEC61B flank
     misc_feature    10662..10730
                     /locus_tag="Sec61B TMD"
                     /label=Sec61B TMD
     misc_feature    10731..10748
                     /locus_tag="Sec61B flank"
                     /label=Sec61B flank
     misc_feature    10749..10778
                     /locus_tag="Linker sequence"
                     /label=Linker sequence
     CDS             10779..11489
                     /codon_start=1
                     /locus_tag="superfolder GFP"
                     /product="GFP variant that folds robustly even when fused
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    11494..11498
                     /locus_tag="NotI and XhoI"
                     /label=NotI and XhoI
     primer_bind     11563..11571
                     /locus_tag="1167_seq(1)"
                     /label=1167_seq
                     /label=1167_seq(1)
     enhancer        11572..12163
                     /locus_tag="WPRE"
                     /label=WPRE
     primer_bind     11572..11585
                     /locus_tag="1167_seq"
                     /label=1167_seq
     CDS             complement(12044..12055)
                     /codon_start=1
                     /locus_tag="Factor Xa site"
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12164..12180)
                     /locus_tag="KS primer"
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
       61 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
      121 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
      181 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      241 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      301 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca
      361 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga
      421 caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct
      481 ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc
      541 cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct
      601 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
      661 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
      721 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
      781 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
      841 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
      901 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
      961 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     1021 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     1081 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     1141 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     1201 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     1261 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     1321 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     1381 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     1441 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     1501 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     1561 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     1621 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     1681 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     1741 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1801 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1861 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1921 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     1981 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     2041 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     2101 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     2161 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     2221 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     2281 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2341 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2401 taactggctt cagcagagcg cagataccaa atactgtctt tctagtgtag ccgtagttag
     2461 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2521 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2581 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2641 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2701 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2761 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2821 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2881 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2941 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3001 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     3061 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
     3121 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3181 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3241 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3301 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3361 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     3421 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
     3481 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
     3541 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
     3601 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
     3661 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
     3721 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
     3781 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
     3841 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
     3901 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
     3961 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
     4021 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
     4081 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
     4141 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
     4201 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
     4261 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
     4321 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
     4381 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
     4441 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
     4501 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     4561 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     4621 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     4681 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     4741 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     4801 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     4861 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     4921 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
     4981 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
     5041 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
     5101 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
     5161 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     5221 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5281 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5341 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     5401 tcccacccca taccctatta ccactgccaa ttacctagtg gtttcattta ctctaaacct
     5461 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     5521 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
     5581 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     5641 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     5701 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     5761 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     5821 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     5881 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     5941 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     6001 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6061 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6121 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     6181 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     6241 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     6301 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     6361 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     6421 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     6481 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     6541 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     6601 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     6661 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     6721 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     6781 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     6841 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     6901 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     6961 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     7021 caagaatcct ggctgtgaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     7081 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     7141 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     7201 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     7261 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     7321 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     7381 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     7441 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     7501 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat
     7561 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     7621 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     7681 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa
     7741 gcttgatatc gaattcggga ggtggtccct gcagttacgc caatgataac ccccgccaga
     7801 aaaatcttag tagccttccc tttttgtttt ccgtgcccca actcggcgga ttgactcggc
     7861 cccttccgga aacacccgaa tcaacttcta gtcaaattat tgttcacgcc gcaatgaccc
     7921 acccctggcc cgcgtctgtg gaactgaccc ctggtgtaca ggagagttcg ctgctgaaag
     7981 tggtcccaaa ggggtactag tttttaagct cccaactccc cctcccccag cgtctggagg
     8041 attccacacc ctcgcaccgc aggggcgagg aagtgggcgg agtccggttt tggcgccagc
     8101 cgctgaggct gccaagcaga aaagccaccg ctgaggagac tccggtcact gtcctcgccc
     8161 cgcctccccc ttccctcccc ttggggacca ccgggcgcca cgccgcgaac ggtaagtgcc
     8221 gcggtcgtcg gcgcctccgc cctcccccta gggccccaat tcccagcggg cgcggcgcgc
     8281 ggcccctccc cccgccgggc gcgcgcccgc tgccccgccc ttcgtggccg cccggcgtgg
     8341 gcggtgccac ccctcccccc ggcggccccc ctgcagccgc gcgcagctcc cggctccctc
     8401 ccccttcgga tgtggcttga gctgtaggcg cggaggggct ccggtgcccg tcagtgggca
     8461 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
     8521 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     8581 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     8641 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
     8701 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
     8761 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
     8821 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
     8881 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     8941 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     9001 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     9061 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     9121 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     9181 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     9241 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     9301 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     9361 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     9421 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     9481 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     9541 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     9601 tttcaggtgt cgtggaattc tcacgcgtct gcaggatatc aagcttgcgg taccgcgggc
     9661 ccgaagctta cgccaccatg agcgagctga ttaaggagaa catgcacatg aagctgtaca
     9721 tggagggcac cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct
     9781 acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct
     9841 tcgacatcct ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg
     9901 gcatccccga cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca
     9961 catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc
    10021 tcatctacaa cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga
    10081 agaaaacact cggctgggag gccttcaccg agacgctgta ccccgctgac ggcggcctgg
    10141 aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacatca
    10201 agaccacata tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg
    10261 tggactacag actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg
    10321 aggtggcagt ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaatggaa
    10381 gcggagctac taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac
    10441 ctggatccgg atccggatct cctggtccga cccccagtgg cactaacgtg ggatcctcag
    10501 ggcgctctcc cagcaaagca gtggccgccc gagcggcggg atccactgtc cggcagagga
    10561 aaaatgccag ctgtgggaca aggagtgcag gccgcacaac ctcggcaggc accgggggga
    10621 tgtggcgatt ctacactcca ggatcctcac ctgggctcaa agttggccct gttccagtat
    10681 tggttatgag tcttctgttc atcgcttctg tatttatgtt gcacatttgg ggcaagtaca
    10741 ctcgttcggg cggatccggt tccggaggtt cgagctcaag caaaggagaa gaacttttca
    10801 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
    10861 tccgtggaga gggtgaaggt gatgctacaa acggaaaact cacccttaaa tttatttgca
    10921 ctactggaaa actacctgtt ccgtggccaa cacttgtcac tactctgacc tatggtgttc
    10981 aatgcttttc ccgttatccg gatcacatga aacggcatga ctttttcaag agtgccatgc
    11041 ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga tgacgggacc tacaagacgc
    11101 gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aagggtattg
    11161 attttaaaga agatggaaac attcttggac acaaactaga gtacaacttt aactcacaca
    11221 atgtatacat cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc
    11281 acaacgttga agatggttcc gttcaactag cagaccatta tcaacaaaat actccaattg
    11341 gcgatggccc tgtcctttta ccagacaacc attacctgtc gacacaatct gtcctttcga
    11401 aagatcccaa cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga
    11461 ttacacatgg catggatgag ctctacaaat aactcgagtc tagagggccc gtttaaaccc
    11521 gctgatcagc ctcgacctgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc
    11581 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
    11641 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
    11701 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
    11761 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
    11821 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
    11881 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
    11941 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
    12001 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
    12061 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
    12121 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
//
