LOCUS       LB_121_pAAV_SC_m4.26_mo 4982 bp ds-DNA     circular     25-OCT-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     LTR             1..141
                     /label="left ITR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    144..175
                     /label="MCS"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    176..187
                     /label="multiple cloning region"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    193..204
                     /label="insertion"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     TATA_signal     204..236
                     /label="4.26"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          214..233
                     /label="SeqPrimer_4.26_frow"
                     /note="sequence: ataatggaagctcgacttcc"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    237..261
                     /label="GGTCGTGAGGCACTGGGCAG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    251..273
                     /label="GCACTGGGCAGGTAAGTATCAAG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    262..362
                     /label="Intron: HBB"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    262..394
                     /label="Chimeric Intron"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    363..394
                     /label="Intron"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(380..402)
                     /label="GTGGACACCTGTGGAGAGAAAGG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    418..480
                     /label="tGFP"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    494..1960
                     /label="Substituted with the library sequence"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    505..526
                     /label="GCGGCCGCATTAGGCACCCCAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    542..561
                     /label="CTTCCGGCTCGTATAATGTG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(542..561)
                     /label="CACATTATACGAGCCGGAAG"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     gene            613..1272
                     /label="CamR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1614..1919
                     /label="ccdB survival"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(1931..1953)
                     /label="CTGCAGACTGGCTGTGTATAAGG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(1953..1967)
                     /label="TCGTCGACGATATCC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(1957..1971)
                     /label="GAATTCGTCGACGAT"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(1961..1975)
                     /label="GGCCGAATTCGTCGA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1988..2209
                     /label="SV40 late poly(A) signal"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2220..2224
                     /label="BamHI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2225..2228
                     /label="SalI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     LTR             complement(2269..2385)
                     /label="right ITR (del trs)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2443..2462
                     /label="GTCAAAGCAACCATAGTACG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2443..2462)
                     /label="CGTACTATGGTTGCTTTGAC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      2477..2783
                     /label="f1 origin"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(2823..2842)
                     /label="GGCCGAAATCGGCAAAATCC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     exon            3302..4159
                     /label="Amp R(bla) ORF"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4163..4182)
                     /label="GAGTAAACTTGGTCTGACAG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      4310..4977
                     /label="pUC origin"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    4957..4976
                     /label="CTTTTGCTGGCCTTTTGCTC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(4957..4976)
                     /label="GAGCAAAAGGCCAGCAAAAG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcGGCCGCg attcacacGG TACCtcagcc agtaAGATCT
      181 gCTAaCCTCG AGTGAGAGGT ACCGagaggg tatataatgg aagctcgact tccagGGGCC
      241 CGGTCGTGAG GCACTGGGCA GGTAAGTATC AAGGTTACAA GACAGGTTTA AGGAGACCAA
      301 TAGAAACTGG GCTTGTCGAG ACAGAGAAGA CTCTTGCGTT TCTGATAGGC ACCTATTGGT
      361 CTTACTGACA TCCACTTTGC CTTTCTCTCC ACAGGTGTCC ACCCTGCAGG CTTAAGcatg
      421 gctagcaaag gagaagaact cttcactgga gttgtcccaa ttcttgttga attagatTGA
      481 tCTAGAGCAT GCACCGGTGA TATCgcggcc gcattaggca ccccaggctt tacactttat
      541 gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct
      601 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      661 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      721 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      781 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
      841 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      901 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      961 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     1021 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     1081 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     1141 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     1201 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     1261 ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg
     1321 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
     1381 agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
     1441 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
     1501 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
     1561 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt
     1621 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     1681 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     1741 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     1801 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     1861 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     1921 tgtcaggctc ccttatacac agccagtctg cagGATATCG TCGACGAATT CGGCCGGCCG
     1981 CTTCGAGCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG
     2041 TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA
     2101 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG
     2161 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAa aatcgataag
     2221 gatccgaccg atgcccttga gagccttcAG CGCTTTGACG gtTaccaccc actccctctc
     2281 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     2341 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
     2401 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     2461 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     2521 tacacttgcc agcgccTtag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     2581 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     2641 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     2701 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     2761 actcttgttc caaactggaa caacactcaa cTctatctcg ggctattctt ttgatttata
     2821 agggattttg ccgatttcgg Tctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     2881 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     2941 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     3001 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     3061 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     3121 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     3181 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     3241 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     3301 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     3361 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     3421 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     3481 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     3541 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     3601 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     3661 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     3721 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     3781 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     3841 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     3901 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     3961 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4021 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4081 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4141 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     4201 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     4261 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4321 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4381 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4441 ggtaactggc ttcagcagag cgcagatacc aaatactgtT cttctagtgt agccgtagtt
     4501 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4561 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4621 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4681 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4741 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4801 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4861 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4921 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4981 gt
//