LOCUS       pssAAV_Seq17_CMV        7148 bp    DNA        circular     26-APR-2024
SOURCE      
  ORGANISM  
COMMENT     ApEinfo:methylated:1
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2504..2524
                     /label="NLS"
                     /ApEinfo_fwdcolor=""#ff0000""
                     /ApEinfo_revcolor=""green""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="NLS"
                     /label="NLS"
                     /ApEinfo_label="NLS"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1..82
                     /dnas_title=""ITR""
                     /vntifkey=""21""
                     /label="ITR"
                     /ApEinfo_fwdcolor=""pink""
                     /ApEinfo_revcolor=""pink""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="ITR"
                     /label="ITR"
                     /ApEinfo_label="ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    4411..4550
                     /dnas_title=""ITR(1)""
                     /vntifkey=""21""
                     /label="ITR(1)"
                     /ApEinfo_label=""ITR""
                     /ApEinfo_fwdcolor=""pink""
                     /ApEinfo_revcolor=""pink""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="ITR(1)"
                     /label="ITR(1)"
                     /ApEinfo_label="ITR(1)"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    5468..6324
                     /dnas_title=""AmpR""
                     /vntifkey=""21""
                     /label="AmpR"
                     /ApEinfo_fwdcolor=""pink""
                     /ApEinfo_revcolor=""pink""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2030..2429
                     /label="beta_globin-intron"
                     /ApEinfo_fwdcolor=""#ffc0cb""
                     /ApEinfo_revcolor=""green""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="beta_globin-intron"
                     /label="beta_globin-intron"
                     /ApEinfo_label="beta_globin-intron"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2447..2476
                     /label="NLS(1)"
                     /ApEinfo_label=""NLS""
                     /ApEinfo_fwdcolor=""#ff0000""
                     /ApEinfo_revcolor=""green""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="NLS(1)"
                     /label="NLS(1)"
                     /ApEinfo_label="NLS(1)"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2477..2497
                     /label="NES"
                     /ApEinfo_fwdcolor=""#ff0000""
                     /ApEinfo_revcolor=""green""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="NES"
                     /label="NES"
                     /ApEinfo_label="NES"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_signal     3274..3861
                     /dnas_title=""WPRE""
                     /vntifkey=""87""
                     /label="WPRE"
                     /locus_tag="WPRE"
                     /label="WPRE"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    3893..4370
                     /note=""poly A""
                     /locus_tag="poly A"
                     /label="poly A"
                     /ApEinfo_label="poly A"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    83..1274
                     /note=""Sequence 17_reverse""
                     /locus_tag="Sequence 17_reverse"
                     /label="Sequence 17_reverse"
                     /ApEinfo_label="Sequence 17_reverse"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1275..1933
                     /note=""CMV Promoter""
                     /locus_tag="CMV Promoter"
                     /label="CMV Promoter"
                     /ApEinfo_label="CMV Promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2525..3235
                     /label="TFP1"
                     /ApEinfo_fwdcolor=""#0080ff""
                     /ApEinfo_revcolor=""green""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="TFP1"
                     /label="TFP1"
                     /ApEinfo_label="TFP1"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtAGATA ATGAGGGTCA GTTCAGAGGC
      181 CATTGCACGG ACCATGACCC CTTGTCAAGA GAGCAGAGCC AATCTACTAT TATTTCTGCT
      241 AAATGGACAT GGTGCATGGG TTAGTGCATC AAACAAGCCC CATCAAGGAA GCTTCTTTTT
      301 TGAAGCAGAT AGCATGTAAC ACAGAGAACC ACAACTGGCC ATGGTGCAGA GAATAAGAGA
      361 CTACAGAATA CTCAGCCCTA GATGTAATGT CTGTCTCACA CTCCTCCCGT CAAGGCTGAG
      421 AGAACTTTGA AGAAAAGGGG GCAGCAAGAA TCAATGGGGA TGACATTAAC TGTGAAACTG
      481 TGATTCACAA CATTGTGGAT ATGGAACCTG AAGAGGCTAT CTCCTGTACC CCAGTGCAGT
      541 GATACAGACA CCAACCCAGC CACAAAACTT TCAACCCAAA ATTTATCCTG TCTACACGAA
      601 GTGTAGGCAC GGGGCAGGGA GCAGAGACTG ACGGAATGGC CAACCAATAA CCGTCCTGAG
      661 ATCCATCCTA TGGGCAAGCC CAATCCCTTA CATTATTAAT GATACTCTGT TATGCTTGAA
      721 GACAGGAGCA TGTTGTTCTC TGAGAGGCTC CACCCAGCAG CTGACTCAGG CAGATACAGA
      781 CACTCACAAT CAATCGGTGG ATGGAGCTTG GAGACTCTTA AAGAAGAATA GGAAGAAAGA
      841 TTGCAGGCCC CGAAGGGGAT AGGAACTCCA CAGGAAGACC AATAGAATCA ACTAACCTGG
      901 ACCCTTGGAG TTCTCAGAGA CTGAACTACC AACCAATGAA CATACACAGG CTGGACCTAG
      961 GCTTCCTTTT ACGAATGTAG CAGATGTGCA GCTTGGTCTT CATATGGGTT CTAAACAACC
     1021 AGAATGAGCG CTATCCCAAA AGCTGTTGCC TGTCTGTGGG ATATGTTCTA GCTGGGCTGC
     1081 CTTGCCTCGC CTCAGTGGGA GAGGAAGACT TGAAGTGGCA GGGTTAGGGA ATACCCATGG
     1141 GACCCCCACC TGCTCAGAAG AAAAGGGGAT GGGGGATGGG GGATGGGGAA AGGATTGTGG
     1201 GAGGGTGTGA GTGGGAGGGG GCAGTGAGTG GGATGTAAAG TGAATAAATT AAAATAAATT
     1261 AGAAAAAAGA AAAGACGCGT ctagttatta atagtaatca attacggggt cattagttca
     1321 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     1381 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1441 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1501 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1561 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1621 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1681 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     1741 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     1801 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
     1861 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
     1921 ccgatccagc ctccgcggat tcgaatcccg gccgggaacg gtgcattgga acgcggattc
     1981 cccgtgccaa gagtgacgta agtaccgcct atagagtcta taggcccaca aaaaatgctt
     2041 tcttctttta atatactttt ttgtttatct tatttctaat actttcccta atctctttct
     2101 ttcagggcaa taatgataca atgtatcatg cctctttgca ccattctaaa gaataacagt
     2161 gataatttct gggttaaggc aatagcaata tttctgcata taaatatttc tgcatataaa
     2221 ttgtaactga tgtaagaggt ttcatattgc taatagcagc tacaatccag ctaccattct
     2281 gcttttattt tatggttggg ataaggctgg attattctga gtccaagcta ggcccttttg
     2341 ctaatcatgt tcatacctct tatcttcctc ccacagctcc tgggcaacgt gctggtctgt
     2401 gtgctggccc atcactttgg caaagaattg ggattcgaac ATCGATACCA TGGACCCAAA
     2461 AAAGAAGCGG AAGGTCCCAA AAAAGAAGCG GAAGGTCGAA TTCccaaaaa agaagcggaa
     2521 ggtcATGGTG AGCAAGGGCG AGGAGACCAC AATGGGCGTA ATCAAGCCCG ACATGAAGAT
     2581 CAAGCTGAAG ATGGAGGGCA ACGTGAATGG CCACGCCTTC GTGATCGAGG GCGAGGGCGA
     2641 GGGCAAGCCC TACGACGGCA CCAACACCAT CAACCTGGAG GTGAAGGAGG GAGCCCCCCT
     2701 GCCCTTCTCC TACGACATTC TGACCACCGC GTTCGCCTAC GGCAACAGGG CCTTCACCAA
     2761 GTACCCCGAC GACATCCCCA ACTACTTCAA GCAGTCCTTC CCCGAGGGCT ACTCTTGGGA
     2821 GCGCACCATG ACCTTCGAGG ACAAGGGCAT CGTGAAGGTG AAGTCCGACA TCTCCATGGA
     2881 GGAGGACTCC TTCATCTACG AGATACACCT CAAGGGCGAG AACTTCCCCC CCAACGGCCC
     2941 CGTGATGCAG AAGAAGACCA CCGGCTGGGA CGCCTCCACC GAGAGGATGT ACGTGCGCGA
     3001 CGGCGTGCTG AAGGGCGACG TCAAGCACAA GCTGCTGCTG GAGGGCGGCG GCCACCACCG
     3061 CGTTGACTTC AAGACCATCT ACAGGGCCAA GAAGGCGGTG AAGCTGCCCG ACTATCACTT
     3121 TGTGGACCAC CGCATCGAGA TCCTGAACCA CGACAAGGAC TACAACAAGG TGACCGTTTA
     3181 CGAGAGCGCC GTGGCCCGCA ACTCCACCGA CGGCATGGAC GAGCTGTACA AGTAAGGATC
     3241 Ctctagagtc gacctgcaga agcttatcga taatcaacct ctggattaca aaatttgtga
     3301 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3361 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3421 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     3481 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     3541 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     3601 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     3661 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     3721 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     3781 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     3841 cctttgggcc gcctccccgc atcgattacc gtcgacctcg agacctagat ctacgggtgg
     3901 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc
     3961 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat
     4021 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt
     4081 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact
     4141 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga
     4201 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc
     4261 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct
     4321 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgattttg
     4381 taggtaacca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
     4441 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     4501 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     4561 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     4621 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4681 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4741 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4801 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     4861 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4921 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga
     4981 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5041 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     5101 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     5161 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     5221 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     5281 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     5341 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5401 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5461 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5521 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5581 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5641 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5701 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5761 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5821 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5881 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5941 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     6001 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6061 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6121 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     6181 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     6241 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6301 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6361 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6421 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6481 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6541 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6601 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     6661 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6721 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6781 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6841 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6901 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6961 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7021 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7081 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7141 tcacatgt     
//
