LOCUS       pssAAV_Seq17_CMV        7157 bp    DNA        circular     26-APR-2024
SOURCE      
  ORGANISM  
COMMENT     ApEinfo:methylated:1
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2504..2524
                     /label="NLS"
                     /ApEinfo_fwdcolor=""#ff0000""
                     /ApEinfo_revcolor=""green""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="NLS"
                     /label="NLS"
                     /ApEinfo_label="NLS"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1..82
                     /dnas_title=""ITR""
                     /vntifkey=""21""
                     /label="ITR"
                     /ApEinfo_fwdcolor=""pink""
                     /ApEinfo_revcolor=""pink""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="ITR"
                     /label="ITR"
                     /ApEinfo_label="ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    4420..4559
                     /dnas_title=""ITR(1)""
                     /vntifkey=""21""
                     /label="ITR(1)"
                     /ApEinfo_label=""ITR""
                     /ApEinfo_fwdcolor=""pink""
                     /ApEinfo_revcolor=""pink""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="ITR(1)"
                     /label="ITR(1)"
                     /ApEinfo_label="ITR(1)"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    5477..6333
                     /dnas_title=""AmpR""
                     /vntifkey=""21""
                     /label="AmpR"
                     /ApEinfo_fwdcolor=""pink""
                     /ApEinfo_revcolor=""pink""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2030..2429
                     /label="beta_globin-intron"
                     /ApEinfo_fwdcolor=""#ffc0cb""
                     /ApEinfo_revcolor=""green""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="beta_globin-intron"
                     /label="beta_globin-intron"
                     /ApEinfo_label="beta_globin-intron"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2447..2476
                     /label="NLS(1)"
                     /ApEinfo_label=""NLS""
                     /ApEinfo_fwdcolor=""#ff0000""
                     /ApEinfo_revcolor=""green""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="NLS(1)"
                     /label="NLS(1)"
                     /ApEinfo_label="NLS(1)"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2477..2497
                     /label="NES"
                     /ApEinfo_fwdcolor=""#ff0000""
                     /ApEinfo_revcolor=""green""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="NES"
                     /label="NES"
                     /ApEinfo_label="NES"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_signal     3283..3870
                     /dnas_title=""WPRE""
                     /vntifkey=""87""
                     /label="WPRE"
                     /locus_tag="WPRE"
                     /label="WPRE"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    3902..4379
                     /note=""poly A""
                     /locus_tag="poly A"
                     /label="poly A"
                     /ApEinfo_label="poly A"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    83..1274
                     /note=""Sequ17""
                     /locus_tag="Sequ17"
                     /label="Sequ17"
                     /ApEinfo_label="Sequ17"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1275..1933
                     /note=""CMV Promoter""
                     /locus_tag="CMV Promoter"
                     /label="CMV Promoter"
                     /ApEinfo_label="CMV Promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2525..3244
                     /dnas_title=""Venus""
                     /vntifkey=""21""
                     /label="Venus"
                     /ApEinfo_fwdcolor=""#ffff00""
                     /ApEinfo_revcolor=""pink""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="Venus"
                     /label="Venus"
                     /ApEinfo_label="Venus"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtCTTTT CTTTTTTCTA ATTTATTTTA
      181 ATTTATTCAC TTTACATCCC ACTCACTGCC CCCTCCCACT CACACCCTCC CACAATCCTT
      241 TCCCCATCCC CCATCCCCCA TCCCCTTTTC TTCTGAGCAG GTGGGGGTCC CATGGGTATT
      301 CCCTAACCCT GCCACTTCAA GTCTTCCTCT CCCACTGAGG CGAGGCAAGG CAGCCCAGCT
      361 AGAACATATC CCACAGACAG GCAACAGCTT TTGGGATAGC GCTCATTCTG GTTGTTTAGA
      421 ACCCATATGA AGACCAAGCT GCACATCTGC TACATTCGTA AAAGGAAGCC TAGGTCCAGC
      481 CTGTGTATGT TCATTGGTTG GTAGTTCAGT CTCTGAGAAC TCCAAGGGTC CAGGTTAGTT
      541 GATTCTATTG GTCTTCCTGT GGAGTTCCTA TCCCCTTCGG GGCCTGCAAT CTTTCTTCCT
      601 ATTCTTCTTT AAGAGTCTCC AAGCTCCATC CACCGATTGA TTGTGAGTGT CTGTATCTGC
      661 CTGAGTCAGC TGCTGGGTGG AGCCTCTCAG AGAACAACAT GCTCCTGTCT TCAAGCATAA
      721 CAGAGTATCA TTAATAATGT AAGGGATTGG GCTTGCCCAT AGGATGGATC TCAGGACGGT
      781 TATTGGTTGG CCATTCCGTC AGTCTCTGCT CCCTGCCCCG TGCCTACACT TCGTGTAGAC
      841 AGGATAAATT TTGGGTTGAA AGTTTTGTGG CTGGGTTGGT GTCTGTATCA CTGCACTGGG
      901 GTACAGGAGA TAGCCTCTTC AGGTTCCATA TCCACAATGT TGTGAATCAC AGTTTCACAG
      961 TTAATGTCAT CCCCATTGAT TCTTGCTGCC CCCTTTTCTT CAAAGTTCTC TCAGCCTTGA
     1021 CGGGAGGAGT GTGAGACAGA CATTACATCT AGGGCTGAGT ATTCTGTAGT CTCTTATTCT
     1081 CTGCACCATG GCCAGTTGTG GTTCTCTGTG TTACATGCTA TCTGCTTCAA AAAAGAAGCT
     1141 TCCTTGATGG GGCTTGTTTG ATGCACTAAC CCATGCACCA TGTCCATTTA GCAGAAATAA
     1201 TAGTAGATTG GCTCTGCTCT CTTGACAAGG GGTCATGGTC CGTGCAATGG CCTCTGAACT
     1261 GACCCTCATT ATCTACGCGT ctagttatta atagtaatca attacggggt cattagttca
     1321 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     1381 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1441 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1501 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1561 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1621 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1681 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     1741 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     1801 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
     1861 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
     1921 ccgatccagc ctccgcggat tcgaatcccg gccgggaacg gtgcattgga acgcggattc
     1981 cccgtgccaa gagtgacgta agtaccgcct atagagtcta taggcccaca aaaaatgctt
     2041 tcttctttta atatactttt ttgtttatct tatttctaat actttcccta atctctttct
     2101 ttcagggcaa taatgataca atgtatcatg cctctttgca ccattctaaa gaataacagt
     2161 gataatttct gggttaaggc aatagcaata tttctgcata taaatatttc tgcatataaa
     2221 ttgtaactga tgtaagaggt ttcatattgc taatagcagc tacaatccag ctaccattct
     2281 gcttttattt tatggttggg ataaggctgg attattctga gtccaagcta ggcccttttg
     2341 ctaatcatgt tcatacctct tatcttcctc ccacagctcc tgggcaacgt gctggtctgt
     2401 gtgctggccc atcactttgg caaagaattg ggattcgaac ATCGATACCA TGGACCCAAA
     2461 AAAGAAGCGG AAGGTCCCAA AAAAGAAGCG GAAGGTCGAA TTCccaaaaa agaagcggaa
     2521 ggtcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2581 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2641 ctacggcaag ctgaccctga agctgatctg caccaccggc aagctgcccg tgccctggcc
     2701 caccctcgtg accaccctgg gctacggcct gcagtgcttc gcccgctacc ccgaccacat
     2761 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2821 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2881 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2941 gcacaagctg gagtacaact acaacagcca caacgtctat atcaccgccg acaagcagaa
     3001 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct
     3061 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3121 ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3181 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3241 gtaaGGATCC tctagagtcg acctgcagaa gcttatcgat aatcaacctc tggattacaa
     3301 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     3361 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     3421 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     3481 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     3541 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3601 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3661 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     3721 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3781 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3841 tcggatctcc ctttgggccg cctccccgca tcgattaccg tcgacctcga gacctagatc
     3901 tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     3961 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     4021 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     4081 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     4141 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     4201 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     4261 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     4321 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     4381 ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
     4441 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     4501 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
     4561 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
     4621 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4681 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4741 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4801 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     4861 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4921 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta
     4981 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     5041 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
     5101 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     5161 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     5221 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     5281 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     5341 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5401 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5461 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5521 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5581 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5641 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5701 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5761 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5821 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5881 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5941 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     6001 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6061 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6121 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6181 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6241 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6301 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6361 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6421 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6481 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6541 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6601 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     6661 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6721 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6781 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6841 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6901 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6961 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7021 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7081 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7141 gccttttgct cacatgt    
//
