LOCUS       pAAV_Seq17_VS33_        7144 bp    DNA        circular     26-APR-2024
SOURCE      
  ORGANISM  
COMMENT     ApEinfo:methylated:1
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     polyA_signal    3889..4366
                     /dnas_title=""BGH polyA""
                     /vntifkey=""25""
                     /label="BGH polyA"
                     /ApEinfo_fwdcolor=""pink""
                     /ApEinfo_revcolor=""pink""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="BGH polyA"
                     /label="BGH polyA"
                     /ApEinfo_label="BGH polyA"
                     /ApEinfo_fwdcolor="#ff3eee"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1..82
                     /dnas_title=""ITR""
                     /vntifkey=""21""
                     /label="ITR"
                     /ApEinfo_fwdcolor=""pink""
                     /ApEinfo_revcolor=""pink""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="ITR"
                     /label="ITR"
                     /ApEinfo_label="ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    4407..4546
                     /dnas_title=""ITR(1)""
                     /vntifkey=""21""
                     /label="ITR(1)"
                     /ApEinfo_label=""ITR""
                     /ApEinfo_fwdcolor=""pink""
                     /ApEinfo_revcolor=""pink""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="ITR(1)"
                     /label="ITR(1)"
                     /ApEinfo_label="ITR(1)"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    5464..6320
                     /dnas_title=""AmpR""
                     /vntifkey=""21""
                     /label="AmpR"
                     /ApEinfo_fwdcolor=""pink""
                     /ApEinfo_revcolor=""pink""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2497..2517
                     /label="NLS"
                     /ApEinfo_fwdcolor=""#ff0000""
                     /ApEinfo_revcolor=""green""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="NLS"
                     /label="NLS"
                     /ApEinfo_label="NLS"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2491..2501
                     /label="2xNLS"
                     /ApEinfo_fwdcolor=""#ff0000""
                     /ApEinfo_revcolor=""green""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="2xNLS"
                     /label="2xNLS"
                     /ApEinfo_label="2xNLS"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2277..2409
                     /note=""Chimeric Intron""
                     /locus_tag="Chimeric Intron"
                     /label="Chimeric Intron"
                     /ApEinfo_label="Chimeric Intron"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2277..2377
                     /note=""Intron: HBB""
                     /locus_tag="Intron: HBB"
                     /label="Intron: HBB"
                     /ApEinfo_label="Intron: HBB"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2378..2409
                     /note=""Intron""
                     /locus_tag="Intron"
                     /label="Intron"
                     /ApEinfo_label="Intron"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_signal     3270..3857
                     /dnas_title=""WPRE""
                     /vntifkey=""87""
                     /label="WPRE"
                     /locus_tag="WPRE"
                     /label="WPRE"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2215..2251
                     /note=""4.26""
                     /locus_tag="4.26"
                     /label="4.26"
                     /ApEinfo_label="4.26"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             2518..3192
                     /dnas_title=""TagRFP""
                     /gene=""TagRFP""
                     /label="TagRFP"
                     /ApEinfo_fwdcolor=""#ff0000""
                     /ApEinfo_revcolor=""pink""
                     /ApEinfo_graphicformat=""arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0""
                     /locus_tag="TagRFP"
                     /label="TagRFP"
                     /ApEinfo_label="TagRFP"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    83..635
                     /note=""480bp""
                     /locus_tag="480bp"
                     /label="480bp"
                     /ApEinfo_label="480bp"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    538..2208
                     /note=""Sequence 15""
                     /locus_tag="Sequence 15"
                     /label="Sequence 15"
                     /ApEinfo_label="Sequence 15"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcGTAGATA ATGAGGGTCA GTTCAGAGGC
      181 CATTGCACGG ACCATGACCC CTTGTCAAGA GAGCAGAGCC AATCTACTAT TATTTCTGCT
      241 AAATGGACAT GGTGCATGGG TTAGTGCATC AAACAAGCCC CATCAAGGAA GCTTCTTTTT
      301 TGAAGCAGAT AGCATGTAAC ACAGAGAACC ACAACTGGCC ATGGTGCAGA GAATAAGAGA
      361 CTACAGAATA CTCAGCCCTA GATGTAATGT CTGTCTCACA CTCCTCCCGT CAAGGCTGAG
      421 AGAACTTTGA AGAAAAGGGG GCAGCAAGAA TCAATGGGGA TGACATTAAC TGTGAAACTG
      481 TGATTCACAA CATTGTGGAT ATGGAACCTG AAGAGGCTAT CTCCTGTACC CCAGTGCAGT
      541 GATACAGACA CCAACCCAGC CACAAAACTT TCAACCCAAA ATTTATCCTG TCTACACGAA
      601 GTGTAGGCAC GGGGCAGGGA GCAGAGACTG ACGGAacgcG TGAGCGTTCC TTTCTCAGCT
      661 CCTTGGCCAG GCAGGCGCTC GCGGCTACCT GCCCTGCTTG GCGCCGCACT GAAGATTCGC
      721 CGCGGCGGAA CAGTTGAGTC TCCATCTGGC TACCAACCCA CCCGAGCTTT CTTCTCCTCC
      781 ATCACCACCT TCCTGCCTCC CCCTCCTCCC CCTCCTTTCC GTCTTCCCTC TCCACCCCCG
      841 CCCCCAATCT CCTCCTCTTT TTCTCACTAC AAGCGGTTGC TGATGCTGAA GCCGAGCGTC
      901 ACTTCGGCTC CCACGGCAGA CATGGCGACA TTGACAGTGG TCCAGCCGCT TACTCTGGAC
      961 AGAGGTAAGG GAGCGGCTGG TCCGTCAGGC CACACAGTCC GCACCGCTTC GCCCTCCGCC
     1021 CTGCCCAGTC CCACAGGGAA GGCAGGATGC GCTCGGGCAG GGGCAGCTGG GGGAGGAGGA
     1081 TCAGCCCAGG AGGGTTTCGC GCCCGGAGCA GGACACTTTC TTCCCCTCTC TCCTCTCCTG
     1141 GTCCCCGCTG CCCCTTCTTG CTGCGCTCTC CGGGGCGTAG GTGCTTGCCA GGGCTCGAAT
     1201 GTTTCAGCTG ACAGTCTCGG TCCCTCGCCG GGTCGCCTCC GGGCCCCGGC TTTGTTGGAG
     1261 AGGATGCGCC GGGACCGCTG AGCGCTGGTG ACTGGAGGTT GAAGAGGGGC AGAGAAAGCT
     1321 CTCCCAGCCA GAGCTTGCTA AGGGACCAGT GGGGAAGCTA GCTGCCAACT GCCGGCGCGG
     1381 GGACCCGAAG GGAGGAAGTT TGTCCCCGCG CTGGAGATGA GGGGTCTGTG CAGCTGTTGT
     1441 GACTGGGAGA TCGCCCCAGG GCGGCAGGGC GCTTGGCTGG GAAGCTGGGT GCAAAGCCGC
     1501 GGGACTGCCT GGCTAGGGCG CTGCCCTCGG GCGAAAGGGC AGAGCAGAGC TCGGGCAACC
     1561 TTCGCTGGCT CAGGGACACC GCATCCGGAG CCGAGCCGGC TTGGCTCCAG CTTCCCGCTC
     1621 GGAGCTGCGG CTCTGGTCTT GGTACTCCCT CAGCCTGCAG TTCCAGGGAG GTAGCAACTC
     1681 CTGTCTCCTG CTCCAGCAAA CCTTGTTCTT CACCAGTCAA GTGTACCTCA GTGCCTGGGC
     1741 TGCAGAAGGC GCGGAGACAA AGCGCAGGCT GGGTAACTGT TTAACTTCTA GCCTGTGACC
     1801 TGTGCGTCCT ACTCTTTGGC TGAAACGGAA AGAGCTTTTG CTCAAACCTT TCCAAACCTT
     1861 TCCAAACCTT TCTCTAGGAT CGGGCCCTGC TGAGTGCACC CGAGTAGTAC CTGGCGGTGG
     1921 ATGGGAGTTT CCTTTCTGCC TCACCAGGTC CAGTTAGTTA TTAGGGCTCC AGAGTCCCCA
     1981 GCTCTGCAGA GAGAGGGCAC CTCTAGCTCT GGGTCCGCAG CGGCGGGGCT TAGGACCCGG
     2041 TGCAGTCATT TGAAAATGCT GTGCTCAGAA GAAAATGATT TAGTCCTTGT CCCTCTAAAA
     2101 TTTCTCTTTC TGGAGGATTA AACTAGCGGC CCAGCAAAGC AAAAAGAGCA TCGTACAGCG
     2161 AGCACAGTGG CAAAGCTGGT AAACTGTATT TGAGGTTCTT AATGTGGAAC CGgtTCGAGa
     2221 gagggtatat aatggaagct cgacttccag GGGCCCGGTC GTGAGGCACT GGGCAGGTAA
     2281 GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA ACTGGGCTTG TCGAGACAGA
     2341 GAAGACTCTT GCGTTTCTGA TAGGCACCTA TTGGTCTTAC TGACATCCAC TTTGCCTTTC
     2401 TCTCCACAGG TGTCCACCCT GCAGGCTTAA GcaatcgatA CCATGGACCC AAAAAAGAAG
     2461 CGGAAGGTCC CAAAAAAGAA GCGGAAGGTC gaattcccaa aaaagaagcg gaaggtcatg
     2521 gtgtctaagg gcgaagagct gattaaggag aacatgcaca tgaagctgta catggagggc
     2581 accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     2641 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
     2701 ctggctacca gcttcatgta cggcagcaga accttcatca accacaccca gggcatcccc
     2761 gacttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
     2821 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
     2881 aacgtcaaga tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca
     2941 ctcggctggg aggccaacac cgagatgctg taccccgctg acggcggcct ggaaggcaga
     3001 agcgacatgg ccctgaagct cgtgggcggg ggccacctga tctgcaactt caagaccaca
     3061 tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggaccac
     3121 agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgaggtggct
     3181 gtggccagat actgcgacct ccctagcaaa ctggggcaca aacttaattg aggatcctct
     3241 agagtcgacc tgcagaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     3301 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3361 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3421 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     3481 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     3541 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     3601 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     3661 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3721 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3781 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3841 tgggccgcct ccccgcatcg attaccgtcg acctcgagac ctagatctac gggtggcatc
     3901 cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca
     3961 gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata
     4021 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg
     4081 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa
     4141 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc
     4201 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca
     4261 tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca
     4321 aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg
     4381 taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc
     4441 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
     4501 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg
     4561 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
     4621 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4681 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4741 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4801 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
     4861 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4921 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta
     4981 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5041 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
     5101 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     5161 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     5221 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     5281 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     5341 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5401 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5461 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5521 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5581 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5641 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5701 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5761 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5821 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5881 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5941 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6001 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6061 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6121 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6181 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6241 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6301 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6361 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6421 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6481 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6541 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6601 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     6661 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6721 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6781 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6841 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6901 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6961 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7021 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7081 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7141 atgt     
//
