LOCUS       EZH2-WT-pHIVEGFP_04              9995 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(5030..6208)
                     /note="pLannotate"
                     /label="EF-1-alpha promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             8965..9825
                     /note="pLannotate"
                     /label="AmpR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1369..2088)
                     /note="pLannotate"
                     /label="EGFP"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     misc_feature    complement(2089..2677)
                     /note="pLannotate"
                     /label="IRES2"
                     /database="addgene"
                     /identity="99.83"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     enhancer        complement(8215..8594)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(8015..8213)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     LTR             complement(7817..8024)
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="addgene"
                     /identity="99.043"
                     /match_length="81.0077519379845"
                     /fragment="True"
                     /other="LTR"
     LTR             complement(839..1151)
                     /note="pLannotate"
                     /label="5' LTR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="49.369085173501574"
                     /fragment="True"
                     /other="LTR"
     LTR             complement(651..838)
                     /note="pLannotate"
                     /label="3' LTR (Delta-U3)"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="80.34188034188034"
                     /fragment="True"
                     /other="LTR"
     misc_feature    complement(7645..7770)
                     /note="pLannotate"
                     /label="HIV-1 Psi"
                     /database="addgene"
                     /identity="98.438"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     misc_feature    complement(6271..6388)
                     /note="pLannotate"
                     /label="cPPT/CTS"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     promoter        8860..8964
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(4968..4991)
                     /note="pLannotate"
                     /label="FLAG"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(2697..2717)
                     /note="pLannotate"
                     /label="SV40 NLS"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    complement(8749..8801)
                     /note="pLannotate"
                     /label="bom"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="37.5886524822695"
                     /fragment="True"
                     /other="misc feature"
     misc_feature    complement(1340..1359)
                     /note="pLannotate"
                     /label="MCS"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="18.867924528301888"
                     /fragment="True"
                     /other="misc feature"
     misc_feature    complement(1153..1172)
                     /note="pLannotate"
                     /label="cPPT/CTS"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="17.094017094017094"
                     /fragment="True"
                     /other="misc feature"
     ncRNA           complement(6861..7196)
                     /note="pLannotate"
                     /label="RRE"
                     /database="infernal"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="ncRNA"
     ncRNA           complement(7511..7593)
                     /note="pLannotate"
                     /label="HIV GSL3"
                     /database="infernal"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="ncRNA"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgc tgctagagat
      661 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
      721 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
      781 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacagtc
      841 cggatgcagc tctcgggcca tgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa
      901 tacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca agcgggtgtt
      961 ctctccttca ttggcttctt ctaccttctc ttgctcaact ggtactagct tgtagcacca
     1021 tccaaaggtc agtggatatc tgatccctgg ccctggtgtg tagttctgcc aatcagggaa
     1081 gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcgtt gggagtgaat
     1141 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
     1201 agtcattggt cttaaaggta cctgaggtgt gactggaaaa cccacctcct cctcctcttg
     1261 tgcttctagc caggcacaat cagcattggt agctgctgta ttgctacttg tgattgctcc
     1321 atgtttttct aggtctcgat cgaggtcgac ggtatcgatg cggccgcttt acttgtacag
     1381 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
     1441 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg
     1501 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
     1561 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt
     1621 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
     1681 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
     1741 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
     1801 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
     1861 ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg
     1921 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
     1981 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
     2041 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg ttgtggccat
     2101 attatcatcg tgtttttcaa aggaaaacca cgtccccgtg gttcgggggg cctagacgtt
     2161 tttttaacct cgactaaaca catgtaaagc atgtgtaccg aggccccaga tcagatccca
     2221 tacaatgggg taccttctgg gcatccttca gccccttgtt gaatacgctt gaggagagcc
     2281 atttgactct ttccacaact atccaactca caacgtggca ctggggttgt gccgcctttg
     2341 caggtgtatc ttatacacgt ggcttttggc cgcagaggca cctgtcgcca ggtggggggt
     2401 tccgctgcct gcaaagggtc gctacagacg ttgtttgtct tcaagaagct tccagaggaa
     2461 ctgcttcctt cacgacattc aacagacctt gcattccttt ggcgagaggg gaaagacccc
     2521 taggaatgct cgtcaagaag acagggccag gtttccgggc cctcacattg ccaaaagacg
     2581 gcaatatggt ggaaaataac atatagacaa acgcacaccg gccttattcc aagcggcttc
     2641 ggccagtaac gttagggggg ggggggaggg agaggggcgg atcctagccc ctattatacc
     2701 tttctcttct tttttggagg gatttccatt tctctttcga tgccgacata cttcagggca
     2761 tcagcctggc tgtatctgta atcaaaaaac agctcttcgc cagtctggat ggctctcttg
     2821 gcaaaaatac ctatcctgtg atcaccatta accatcataa cttttgcata gcagtttgga
     2881 tttaccgaat gatttgcaaa acgaattttg ttacccttgc gggttgcatc caccacaaaa
     2941 tcattgttca agttgaacag aaagctgcac atgtatttat catacacttt ccctcttctg
     3001 tcagcttcat cttgagaaat aatctctcca cagtattctg agatgaattc atttttctgc
     3061 acaggatctt tgataaaaat cccccagcct gccacgtcag atggtgccag caatagatgc
     3121 tttttggagc cccgctgaat actgcagttc ttgcaggaca catttttact gtcccaatgg
     3181 tcagcggctc cacaagtaag acagaggtca gggtcacact ctcggacagc caggtagcac
     3241 gggcactgct tggtgttgca ctgtgctttg cagcggcatc ccggaaagcg gttttgacac
     3301 tctgaactac attgacaaaa cttttcacaa aaattttgtg ctatcacaca agggcacgaa
     3361 ctgtcacaag gctgccgtgg atgatcacag ggttgatagt tgtaaacatg gttagaggag
     3421 ccgtcctttt tcagctgtat ctttctgcag tgtgcagccc acaaccggtg tttcctcttc
     3481 tttttccttg gaggagtatc cacatcctca gcgggagctg gagctatgat gctagattct
     3541 ttgactctaa actcatacac ctgtctacat gttttggtcc caattaacct agcaatggca
     3601 cagaaattgt catagtaagt gccaatgagg actctaaaca ttgaggcttc agcaccactc
     3661 cactccacat tctcaggagg ttcaatattt ggcttcatct ttattggtgt ttgacaccga
     3721 gaatttgctt cagaggagct cgaagtttca tctttcttct cttcttcttc tttatcattg
     3781 ttctctcccc ccgtttcagt ccctgcttcc ctatcactgt ctgtatcctt tgattccagc
     3841 acattaatgg tgggggtgct gggcctgcta ctgttattgg gaagccgtcc tcttctgcgg
     3901 cctcctggac gttttggtgg ggtctttatc cgctcagcgg tgagagcagc agcaaactcc
     3961 tttgctccct ccaaatgctg gtaacactgt ggtccacaag gtttgttgtc tagagctgtt
     4021 tctgtgttct tccgcttata agtgttgggt gttgcatgaa aagaataatt gcacttacga
     4081 tgtaggaagc agtcatattt aaaacatcgc ctacagaaaa gcgtatgaaa ggagtgtaag
     4141 ctttgctctc tctgaacaga tttagcattt ggtccatcta tgttgggggt acattcagga
     4201 ggaagtgcgc ctgggagctg ctgttcggtg agttctttat atttttcctt tagttcttct
     4261 gctgtgccct tatctggaaa cattgaggaa atggcttcaa aaattttatc agaaggaaat
     4321 ttccgaggtg ggcggctttc tttatcatct cggtgatcct ccagatcttt ctgcttttct
     4381 tctctttctt caggatcgtc tccatcatca tcatcgtcat catcattata ttgaccaagg
     4441 gcattcacca actccacaaa aatttcatca tttataaacc cacattctct atccccgtgt
     4501 actttcccat cataattttt tattagttct tcaatgaaag taccatcctg atctaaaact
     4561 tcatctccca tataaggaat gttatgtaaa acagtttcat cttccaccat aaaattctgc
     4621 tgtaggggtg accaagaata cattatgggt actgaagcaa ctgcattcag agtctttaat
     4681 gggatgactt gtgttggaaa atccaagtca ctggtcaccg aacactccct agtcccgcgc
     4741 aatgagctca cagaagtcag gatgtgcaca ggctgtatcc ttcgctgttt ccattcttgg
     4801 tttaagattt ccgttctttc caaaattttc tgacgattgg aactaaacat actctttact
     4861 tcatcagctc gtctgaacct cttgagctgt ctcagtcgca tgtactctga ttttacacgc
     4921 ttccgccaac aaactggtcc cttctcagat ttcttcccag tctggccctt gtcgtcatcg
     4981 tctttgtagt ccatggtggg tactctagaa tagttaactc agcggccgct cacgacacct
     5041 gaaatggaag aaaaaaactt tgaaccactg tctgaggctt gagaatgaac caagatccaa
     5101 actcaaaaag ggcaaattcc aaggagaatt acatcaagtg ccaagctggc ctaacttcag
     5161 tctccaccca ctcagtgtgg ggaaactcca tcgcataaaa cccctccccc caacctaaag
     5221 acgacgtact ccaaaagctc gagaactaat cgaggtgcct ggacggcgcc cggtactccg
     5281 tggagtcaca tgaagcgacg gctgaggacg gaaaggccct tttcctttgt gtgggtgact
     5341 cacccgcccg ctctcccgag cgccgcgtcc tccattttga gctccctgca gcagggccgg
     5401 gaagcggcca tctttccgct cacgcaactg gtgccgaccg ggccagcctt gccgcccagg
     5461 gcggggcgat acacggcggc gcgaggccag gcaccagagc aggccggcca gcttgagact
     5521 acccccgtcc gattctcggt ggccgcgctc gcaggccccg cctcgccgaa catgtgcgct
     5581 gggacgcacg ggccccgtcg ccgcccgcgg ccccaaaaac cgaaatacca gtgtgcagat
     5641 cttggcccgc atttacaaga ctatcttgcc agaaaaaaag cgtcgcagca ggtcatcaaa
     5701 aattttaaat ggctagagac ttatcgaaag cagcgagaca ggcgcgaagg tgccaccaga
     5761 ttcgcacgcg gcggccccag cgcccaggcc aggcctcaac tcaagcacga ggcgaagggg
     5821 ctccttaagc gcaaggcctc gaactctccc acccacttcc aacccgaagc tcgggatcaa
     5881 gaatcacgta ctgcagccag gtggaagtaa ttcaaggcac gcaagggcca taacccgtaa
     5941 agaggccagg cccgcgggaa ccacacacgg cacttacctg tgttctggcg gcaaacccgt
     6001 tgcgaaaaag aacgttcacg gcgactactg cacttatata cggttctccc ccaccctcgg
     6061 gaaaaaggcg gagccagtac acgacatcac tttcccagtt taccccgcgc caccttctct
     6121 aggcaccggt tcaattgccg acccctcccc ccaacttctc ggggactgtg ggcgatgtgc
     6181 gctctgccca ctgacgggca ccggagcctc acgctagagc gggcccggta ctaaccaaac
     6241 tggatctctg ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg
     6301 taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta
     6361 ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt gtctgcagaa
     6421 ttggcgcacg cagtgccgat ccgttcacta atcgaatgga tctgtctctg tctctctctc
     6481 caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct
     6541 gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa
     6601 ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata taccacagcc
     6661 aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt
     6721 gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt
     6781 aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag
     6841 atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc
     6901 aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg
     6961 cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag
     7021 ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg
     7081 tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca
     7141 aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg
     7201 gtgctactcc taatggttca atttttacta ctttatattt atataattca cttctccaat
     7261 tgtccctcat atctcctcct ccaggtctga agatcagcgg ccggccgctt gctgtgcggt
     7321 ggtcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat
     7381 ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc
     7441 ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga
     7501 tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat
     7561 atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga
     7621 tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc
     7681 gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc
     7741 ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc
     7801 cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct gagggatctc
     7861 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
     7921 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
     7981 tctaaccaga gagacccagt acaggcaaaa cgcgctgctt atatagacct cccaccgtac
     8041 acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg
     8101 ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc
     8161 ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt
     8221 aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta
     8281 ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg
     8341 catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg
     8401 acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa
     8461 tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg
     8521 gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt
     8581 caataatcaa tgtcaacgcg tatatctggc ccgtacatcg cgaagcagcg caaaacgcct
     8641 aaccctaagc agattcttca tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat
     8701 tttgctcgcg cactactcag cgacctccaa cacacaagca gggagcagat actggcttaa
     8761 ctatgcggca tcagagcaga ttgtactgag agtgcaccat aggggatcgg gagatctccc
     8821 gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     8881 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     8941 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     9001 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     9061 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     9121 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     9181 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     9241 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     9301 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     9361 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     9421 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     9481 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     9541 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     9601 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     9661 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     9721 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     9781 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     9841 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     9901 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     9961 gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
