LOCUS       pSpy1C                  4917 bp DNA     circular SYN 18-MAR-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...19a-f1zml09
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 18, 2024 from SnapGene Viewer 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3719..3755
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
                     /organism="synthetic DNA construct"
     source          3850..4884
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
                     /organism="synthetic DNA construct"
     primer_bind     complement(5..21)
                     /label=M13_r
     misc_feature    complement(24..45)
                     /direction=LEFT
                     /label=pJRS-R
                     /label=nonstandard type: motif
     terminator      complement(352..394)
                     /note="/created_by=g_geneious"
     -35_signal      482..487
                     /label=-35
     -10_signal      505..510
                     /label=-10
     CDS             624..1274
                     /codon_start=1
                     /label=Cm(R)
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     terminator      1601..1634
                     /note="/created_by=g_geneious"
     CDS             complement(1649..2344)
                     /codon_start=1
                     /label=REP A
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNSSDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
     CDS             complement(2413..2571)
                     /codon_start=1
                     /label=REP C
                     /translation="MVISESKKRVMISLTKEQDKKLTDMAKQKGFSKSAVAALAIEEYA
                     RKESEQKK"
     terminator      complement(3094..3146)
                     /note="/created_by=g_geneious"
     terminator      3720..3754
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     3790..3807
                     /label=M13_f
     misc_feature    3821..3826
                     /label=EcoRI
                     /label=nonstandard type: restriction site
                     /note="/Recognition_pattern=G^AATTC"
                     /note="/annotation_group=GAATTC"
     misc_feature    3836..3841
                     /label=XbaI
                     /label=nonstandard type: restriction site
                     /note="/Recognition_pattern=T^CTAGA"
                     /note="/annotation_group=TCTAGA"
     misc_feature    3844..3849
                     /label=NgoMIV
                     /label=nonstandard type: restriction site
                     /note="/Recognition_pattern=G^CCGGC"
                     /note="/annotation_group=GCCGGC"
     protein_bind    3916..3937
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3952..3982
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3990..4006
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4024..4035
                     /label=RBS
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4042..4719
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      4764..4835
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4851..4878
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    4895..4900
                     /label=SpeI
                     /label=nonstandard type: restriction site
                     /note="/Recognition_pattern=A^CTAGT"
                     /note="/annotation_group=ACTAGT"
     misc_feature    4902..4907
                     /label=PstI
                     /label=nonstandard type: restriction site
                     /note="/Recognition_pattern=CTGCA^G"
                     /note="/annotation_group=CTGCAG"
ORIGIN
        1 atgtgtcata gctgtttcct gcaaagaatg gtgatgacaa ttgtacaggt tatcaataaa
       61 gccattgata ctctcattct tatcccgtat ttattaactt ctcctagtat tcgtaattat
      121 catggctgtc atggcgcatt aacggaataa agggtgtgct taaatcgggc cattttgcgt
      181 aataagaaaa aggattaatt atgagcgaat tgaattaata ataaggtaat agatttacat
      241 tagaaaatga aaggggattt tatgcgtgag aatgttacag tctatcccgg caatagttac
      301 ccttattatc aagataagaa agaaaaggat ttttcgctac gctcaaatcc tttaaaaaaa
      361 cacaaaagac cacatttttt aatgtggtct ttattcttca actaaagcac ccattagttc
      421 aacaaacgaa aattggataa agtgggatat ttttaaaata tatatttatg ttacagtaat
      481 attgactttt aaaaaaggat tgattctaat gaagaaagca gacaagtaag cctcctaaat
      541 tcactttaga taaaaattta ggaggcatat caaatgaact ttaataaaat tcactttaga
      601 taaaaattta ggaggcatat caaatgaact ttaataaaat tgatttagac aattggaaga
      661 gaaaagagat atttaatcat tatttgaacc aacaaacgac ttttagtata accacagaaa
      721 ttgatattag tgttttatac cgaaacataa aacaagaagg atataaattt taccctgcat
      781 ttattttctt agtgacaagg gtgataaact caaatacagc ttttagaact ggttacaata
      841 gcgacggaga gttaggttat tgggataagt tagagccact ttatacaatt tttgatggtg
      901 tatctaaaac attctctggt atttggactc ctgtaaagaa tgacttcaaa gagttttatg
      961 atttatacct ttctgatgta gagaaatata atggttcggg gaaattgttt cccaaaacac
     1021 ctatacctga aaatgctttt tctctttcta ttattccatg gacttcattt actgggttta
     1081 acttaaatat caataataat agtaattacc ttctacccat tattacagca ggaaaattca
     1141 ttaataaagg taattcaata tatttaccgc tatctttaca ggtacatcat tctgtttgtg
     1201 atggttatca tgcaggattg tttatgaact ctattcagga attgtcagat aggcctaatg
     1261 actggctttt ataatatgag ataatgccga ctgtactttt tacagtcggt tttctaatgt
     1321 cactaacctg ccccgttagt tgaagaaggt ttttatatta cagctccaga tccatatcct
     1381 tctttttctg aaccgacttc tcctttttcg cttctttatt ccaattgctt tattgacgtt
     1441 gagcctcgga acccttaaca atcccaaaac ttgtcgactt tcgtcagggg ggcttttatt
     1501 tattcaataa tccctcctct caataaatct attgttgtac ttaattcaac ttccatttct
     1561 ctgtatcttt caatacgctc ttttaagtcc ttaatttctt tttttaattc ctcattttca
     1621 gcaaataact ctttttcttt gtttgtcatt ttatttcccc cgtttcagca tcaagaacct
     1681 ttgcataact tgctctatat ccacactgat aattgccctc aaaccataat ctaaaggcgc
     1741 tagagtttgt tgaaacaata tcttttacat cattcgtatt taaaattcca aactccgctc
     1801 ccctaaggcg aataaaagcc attaaatctt ttgtatttac caaattatag tcatccacta
     1861 tatctaaaag taaattcttc aattctcttt tttggctttc atcaagtgtt atatagcggt
     1921 caatatcaaa atcattaatg ttcaaaatat cttttttgtc gtatatatgt ttattcttag
     1981 caatagcgtc ctttgattca tgagtcaaat attcatatga acctttgata taatcaagta
     2041 tctcaacatg agcaactgaa ctattcccca attttcgctt aatcttgttc ctaacgcttt
     2101 ctattgttac aggatttcgt gcaatatata taacgtgata gtgtggtttt ttatagtgct
     2161 ttccatttcg tataacatca ctactattcc atgtatcttt atcttttttt tcgtccatat
     2221 cgtgtaaagg actgacagcc atagatacgc ccaaactctc taatttttct ttccaatcat
     2281 taggaattga gtcaggatat aataaaaatc caaaatttct agctttagta tttttaatag
     2341 ccatgatata ttaccttatc aaaaacaagt agcgaaaact cgtatccttc taaaaacgcg
     2401 agctttcgct tatttttttt gttctgattc ctttcttgca tattcttcta tagctaacgc
     2461 cgcaaccgca gattttgaaa aacctttttg tttcgccata tctgttaatt ttttatcttg
     2521 ctcttttgtc agagaaatca taactctttt tttcgattct gaaatcacca tttaaaaaac
     2581 tccaatcaaa taattttata aaattagtgt atcactttgt aatcataaaa acaacaataa
     2641 agctacttaa atatagattt ataaaaaacg ttggcgaaaa cgttggcgat tcgttggcga
     2701 ttgaaaaacc cctcaaaccc ttgagccagt tgggatagag cgtttttggc acaaaaattg
     2761 gcactcggca cttaatgggg ggtcgtagta cggaagcaaa attcgcttcc tttcccccca
     2821 tttttttcca aattccaaat ttttttcaaa aattttccag cgctaccgct cggcaaaatt
     2881 gcaagcaatt tttaaaatca aacccatgag ggaatttcat tccctcaaac tcccttgagc
     2941 ctcctccaac cgaaatagaa ggacgctgcg cttattattt cattcagtca tcggctttca
     3001 taatctaaca gacaacatct tcgctgcaaa gcacgctacg ctcaagggct tttacgctac
     3061 gataacgcct gttttaacga ttatgccgat aactaaacga aataaacgct aaaacgtctc
     3121 agaaacgatt ttgagacgtt ttaataaaaa atcgaccgaa aaataaatat aaatcggata
     3181 tacaatcggc aattgacgaa actgcaaaat atcctgtaaa ggatacggat tttatgaccg
     3241 atgatgaaga aaagaatttg aaacgtttgt ctgatttgga ggaaggttta caccgtaaaa
     3301 ggttaatctc ctatggtggt ttgttaaaag aaatacataa aaaattaaac cttgatgaca
     3361 cagaagaagg cgatttgatt catacagatg atgacgaaaa agccgatgaa gatggatttt
     3421 ctattattgc aatgtggaat tgggaacgga aaaattattt tattaaagag tagttcaaca
     3481 aacgggccag tttgttgaag attagatgct ataattgtta ttaaaaggat tgaaggatgc
     3541 ttaggaagac gagttattaa tagctgaata agaacggtgc tctccaaata ttcttattta
     3601 gaaaacgaaa tctaaaatta tctgaaaagg gaatgagaat agtgaatgga ccaataataa
     3661 tgactagaga agaaagaatg aagattgttc atgaaattaa ggaacgaata ttggataaca
     3721 taaaaaacgc ccggcggcaa ccgagcgttc tgaattatgg ggatgatgtt aaggctattg
     3781 gtgtttatgg taaaacgacg gccagtcagc gagaccagct gaattcaaaa aaaaatctag
     3841 atggccggcg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     3901 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     3961 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacatacta
     4021 gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt
     4081 ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt
     4141 gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg
     4201 ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt
     4261 aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg
     4321 gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg
     4381 caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgatggt
     4441 ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa
     4501 gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac
     4561 gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac
     4621 aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag
     4681 tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt
     4741 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     4801 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     4861 cgggtgggcc tttctgcgtt tataaccggt taatactagt actgcagtga aggtctc
//
