LOCUS       pGYL17                  4933 bp ds-DNA     circular     07-AUG-2023
DEFINITION  .
KEYWORDS    "accession:AY738222" "creator:Promega" "marker:AmpR"
            "organism:Mammalian Cells"
COMMENT     Promoterless vector for measuring the activity of promoter and
            enhancer sequences with a luciferase assay.
FEATURES             Location/Qualifiers
     misc_feature    20..399
                     /label="Alb-10K Enh"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    438..755
                     /label="minimal albpro_318bp"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(776..795)
                     /label="FF luc 5' reverse"
                     /note="sequence: TCCATGGTGGCTTTACCAAC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             791..2443
                     /label="luciferase"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1025..1044
                     /label="ON8047"
                     /note="sequence: GTGGTGTGCAGCGAGAATAG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(1104..1125)
                     /label="ON8048"
                     /note="sequence: CGCTCGTTGTAGATGTCGTTAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2305..2324
                     /label="ON8045"
                     /note="sequence: GGTTACAACCGCCAAGAAGC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2347..2366)
                     /label="ON8046"
                     /note="sequence: GTCCTTTAGGCACCTCGTCC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     polyA_signal    2487..2608
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     rep_origin      complement(3027..3615)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          3476..3495
                     /label="ON7888_AAVtiter_ORI_F"
                     /note="sequence: TTGGTATCTGCGCTCTGCTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(3560..3579)
                     /label="ON7889_AAVTiter_ORI_R"
                     /note="sequence: GCAAACAAAAAAACCACCGC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             complement(3815..4606)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(4607..4675)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     polyA_signal    4780..4828
                     /label="poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4842..4933
                     /label="pause site"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          4882..4901
                     /label="RV3primer"
                     /note="sequence: CTAGCAAAATAGGCTGTCCC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 GGCCTAACTG GCCGGTACCG ATTACATTAC ACATTCTGCA AGCATAGCAC AGAGCAATGT
       61 TCTACTTTAA TTACTTTCAT TTTCTTGTAT CCTCACAGCC TAGAAAATAA CCTGCGTTAC
      121 AGCATCCACT CAGTATCCCT TGAGCATGAG GTGACACTAC TTAACATAGG GACGAGATGG
      181 TACTTTGTGT CTCCTGCTCT GTCAGCAGGG CACTGTACTT GCTGATACCA GGGAATGTTT
      241 GTTCTTAAAT ACCATCATTC CGGACGTGTT TGCCTTGGCC AGTTTTCCAT GTACATGCAG
      301 AAAGAAGTTT GGACTGATCA ATACAGTCCT CTGCCTTTAA AGCAATAGGA AAAGGCCAAC
      361 TTGTCTACGT TTAGTATGTG GCTGTAGAAA GGGTATAGAC TCGAGGATAT CAAGATCTGG
      421 CCTCGGCGGC CAAGCTTTGC TCAAATGGGA GACAAAGAGA TTAAGCTCTT ATGTAAAATT
      481 TGCTGTTTTA CATAACTTTA ATGAATGGAC AAAGTCTTGT GCATGGGGGT GGGGGTGGGG
      541 TTAGAGGGGA ACAGCTCCAG ATGGCAAACA TACGCAAGGG ATTTAGTCAA ACAACTTTTT
      601 GGCAAAGATG GTATGATTTT GTAATGGGGT AGGAACCAAT GAAATGCGAG GTAAGTATGG
      661 TTAATAATCT ACAGTTATTG GTTAAAGAAG TATATTAGAG CGAGTCTTTC TGCACACAGA
      721 TCACCTTCCT ATCAACCCCA CTAGCCTCTG GCAAAAAGCT TGGCAATCCG GTACTGTTGG
      781 TAAAGCCACC ATGGAAGATG CCAAAAACAT TAAGAAGGGC CCAGCGCCAT TCTACCCACT
      841 CGAAGACGGG ACCGCCGGCG AGCAGCTGCA CAAAGCCATG AAGCGCTACG CCCTGGTGCC
      901 CGGCACCATC GCCTTTACCG ACGCACATAT CGAGGTGGAC ATTACCTACG CCGAGTACTT
      961 CGAGATGAGC GTTCGGCTGG CAGAAGCTAT GAAGCGCTAT GGGCTGAATA CAAACCATCG
     1021 GATCGTGGTG TGCAGCGAGA ATAGCTTGCA GTTCTTCATG CCCGTGTTGG GTGCCCTGTT
     1081 CATCGGTGTG GCTGTGGCCC CAGCTAACGA CATCTACAAC GAGCGCGAGC TGCTGAACAG
     1141 CATGGGCATC AGCCAGCCCA CCGTCGTATT CGTGAGCAAG AAAGGGCTGC AAAAGATCCT
     1201 CAACGTGCAA AAGAAGCTAC CGATCATACA AAAGATCATC ATCATGGATA GCAAGACCGA
     1261 CTACCAGGGC TTCCAAAGCA TGTACACCTT CGTGACTTCC CATTTGCCAC CCGGCTTCAA
     1321 CGAGTACGAC TTCGTGCCCG AGAGCTTCGA CCGGGACAAA ACCATCGCCC TGATCATGAA
     1381 CAGTAGTGGC AGTACCGGAT TGCCCAAGGG CGTAGCCCTA CCGCACCGCA CCGCTTGTGT
     1441 CCGATTCAGT CATGCCCGCG ACCCCATCTT CGGCAACCAG ATCATCCCCG ACACCGCTAT
     1501 CCTCAGCGTG GTGCCATTTC ACCACGGCTT CGGCATGTTC ACCACGCTGG GCTACTTGAT
     1561 CTGCGGCTTT CGGGTCGTGC TCATGTACCG CTTCGAGGAG GAGCTATTCT TGCGCAGCTT
     1621 GCAAGACTAT AAGATTCAAT CTGCCCTGCT GGTGCCCACA CTATTTAGCT TCTTCGCTAA
     1681 GAGCACTCTC ATCGACAAGT ACGACCTAAG CAACTTGCAC GAGATCGCCA GCGGCGGGGC
     1741 GCCGCTCAGC AAGGAGGTAG GTGAGGCCGT GGCCAAACGC TTCCACCTAC CAGGCATCCG
     1801 CCAGGGCTAC GGCCTGACAG AAACAACCAG CGCCATTCTG ATCACCCCCG AAGGGGACGA
     1861 CAAGCCTGGC GCAGTAGGCA AGGTGGTGCC CTTCTTCGAG GCTAAGGTGG TGGACTTGGA
     1921 CACCGGTAAG ACACTGGGTG TGAACCAGCG CGGCGAGCTG TGCGTCCGTG GCCCCATGAT
     1981 CATGAGCGGC TACGTTAACA ACCCCGAGGC TACAAACGCT CTCATCGACA AGGACGGCTG
     2041 GCTGCACAGC GGCGACATCG CCTACTGGGA CGAGGACGAG CACTTCTTCA TCGTGGACCG
     2101 GCTGAAGAGC CTGATCAAAT ACAAGGGCTA CCAGGTAGCC CCAGCCGAAC TGGAGAGCAT
     2161 CCTGCTGCAA CACCCCAACA TCTTCGACGC CGGGGTCGCC GGCCTGCCCG ACGACGATGC
     2221 CGGCGAGCTG CCCGCCGCAG TCGTCGTGCT GGAACACGGT AAAACCATGA CCGAGAAGGA
     2281 GATCGTGGAC TATGTGGCCA GCCAGGTTAC AACCGCCAAG AAGCTGCGCG GTGGTGTTGT
     2341 GTTCGTGGAC GAGGTGCCTA AAGGACTGAC CGGCAAGTTG GACGCCCGCA AGATCCGCGA
     2401 GATTCTCATT AAGGCCAAGA AGGGCGGCAA GATCGCCGTG TAATAATTCT AGAGTCGGGG
     2461 CGGCCGGCCG CTTCGAGCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC
     2521 TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT
     2581 AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA
     2641 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAA
     2701 AATCGATAAG GATCCGTCGA CCGATGCCCT TGAGAGCCTT CAACCCAGTC AGCTCCTTCC
     2761 GGTGGGCGCG GGGCATGACT ATCGTCGCCG CACTTATGAC TGTCTTCTTT ATCATGCAAC
     2821 TCGTAGGACA GGTGCCGGCA GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG
     2881 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA
     2941 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
     3001 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
     3061 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
     3121 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
     3181 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT
     3241 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
     3301 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
     3361 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
     3421 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT
     3481 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC
     3541 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA
     3601 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
     3661 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
     3721 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
     3781 GACAGCGGCC GCAAATGCTA AACCACTGCA GTGGTTACCA GTGCTTGATC AGTGAGGCAC
     3841 CGATCTCAGC GATCTGCCTA TTTCGTTCGT CCATAGTGGC CTGACTCCCC GTCGTGTAGA
     3901 TCACTACGAT TCGTGAGGGC TTACCATCAG GCCCCAGCGC AGCAATGATG CCGCGAGAGC
     3961 CGCGTTCACC GGCCCCCGAT TTGTCAGCAA TGAACCAGCC AGCAGGGAGG GCCGAGCGAA
     4021 GAAGTGGTCC TGCTACTTTG TCCGCCTCCA TCCAGTCTAT GAGCTGCTGT CGTGATGCTA
     4081 GAGTAAGAAG TTCGCCAGTG AGTAGTTTCC GAAGAGTTGT GGCCATTGCT ACTGGCATCG
     4141 TGGTATCACG CTCGTCGTTC GGTATGGCTT CGTTCAACTC TGGTTCCCAG CGGTCAAGCC
     4201 GGGTCACATG ATCACCCATA TTATGAAGAA ATGCAGTCAG CTCCTTAGGG CCTCCGATCG
     4261 TTGTCAGAAG TAAGTTGGCC GCGGTGTTGT CGCTCATGGT AATGGCAGCA CTACACAATT
     4321 CTCTTACCGT CATGCCATCC GTAAGATGCT TTTCCGTGAC CGGCGAGTAC TCAACCAAGT
     4381 CGTTTTGTGA GTAGTGTATA CGGCGACCAA GCTGCTCTTG CCCGGCGTCT ATACGGGACA
     4441 ACACCGCGCC ACATAGCAGT ACTTTGAAAG TGCTCATCAT CGGGAATCGT TCTTCGGGGC
     4501 GGAAAGACTC AAGGATCTTG CCGCTATTGA GATCCAGTTC GATATAGCCC ACTCTTGCAC
     4561 CCAGTTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC GGGGTGTGCA AAAACAGGCA
     4621 AGCAAAATGC CGCAAAGAAG GGAATGAGTG CGACACGAAA ATGTTGGATG CTCATACTCG
     4681 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTACTA GTACGTCTCT CAAGGATAAG
     4741 TAAGTAATAT TAAGGTACGG GAGGTATTGG ACAGGCCGCA ATAAAATATC TTTATTTTCA
     4801 TTACATCTGT GTGTTGGTTT TTTGTGTGAA TCGATAGTAC TAACATACGC TCTCCATCAA
     4861 AACAAAACGA AACAAAACAA ACTAGCAAAA TAGGCTGTCC CCAGTGCAAG TGCAGGTGCC
     4921 AGAACATTTC TCT
//