LOCUS       pGYL18                  4213 bp ds-DNA     circular     07-AUG-2023
DEFINITION  .
KEYWORDS    "accession:AY738226" "creator:Promega" "marker:AmpR"
            "organism:Mammalian Cells"
COMMENT     Promoterless vector for measuring the activity of promoter and
            enhancer sequences with a Renilla luciferase assay.
FEATURES             Location/Qualifiers
     misc_feature    20..399
                     /label="Alb-10K Enh"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    438..755
                     /label="minimal albpro_318bp"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          511..530
                     /label="ON8755"
                     /note="sequence: AAAGTCTTGTGCATGGGGGT"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             791..1726
                     /label="hRluc"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(857..876)
                     /label="5'-hRluc reverse sequencing primer "
                     /note="sequence: ACGTTCATTTGCTTGCAGCG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          1462..1481
                     /label="ON8049"
                     /note="sequence: TCTCGTTAAGGGAGGCAAGC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1507..1526)
                     /label="ON8050"
                     /note="sequence: CCCGAAGGTAGGCGTTGTAG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     polyA_signal    1767..1888
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1984..2003)
                     /label="ON8756"
                     /note="sequence: CGGTCGACGGATCCTTATCG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      complement(2307..2895)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(3095..3886)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(3887..3955)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     polyA_signal    4060..4108
                     /label="poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4122..4213
                     /label="pause site"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          4162..4181
                     /label="RV3primer"
                     /note="sequence: CTAGCAAAATAGGCTGTCCC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 GGCCTAACTG GCCGGTACCG ATTACATTAC ACATTCTGCA AGCATAGCAC AGAGCAATGT
       61 TCTACTTTAA TTACTTTCAT TTTCTTGTAT CCTCACAGCC TAGAAAATAA CCTGCGTTAC
      121 AGCATCCACT CAGTATCCCT TGAGCATGAG GTGACACTAC TTAACATAGG GACGAGATGG
      181 TACTTTGTGT CTCCTGCTCT GTCAGCAGGG CACTGTACTT GCTGATACCA GGGAATGTTT
      241 GTTCTTAAAT ACCATCATTC CGGACGTGTT TGCCTTGGCC AGTTTTCCAT GTACATGCAG
      301 AAAGAAGTTT GGACTGATCA ATACAGTCCT CTGCCTTTAA AGCAATAGGA AAAGGCCAAC
      361 TTGTCTACGT TTAGTATGTG GCTGTAGAAA GGGTATAGAC TCGAGGATAT CAAGATCTGG
      421 CCTCGGCGGC CAAGCTTTGC TCAAATGGGA GACAAAGAGA TTAAGCTCTT ATGTAAAATT
      481 TGCTGTTTTA CATAACTTTA ATGAATGGAC AAAGTCTTGT GCATGGGGGT GGGGGTGGGG
      541 TTAGAGGGGA ACAGCTCCAG ATGGCAAACA TACGCAAGGG ATTTAGTCAA ACAACTTTTT
      601 GGCAAAGATG GTATGATTTT GTAATGGGGT AGGAACCAAT GAAATGCGAG GTAAGTATGG
      661 TTAATAATCT ACAGTTATTG GTTAAAGAAG TATATTAGAG CGAGTCTTTC TGCACACAGA
      721 TCACCTTCCT ATCAACCCCA CTAGCCTCTG GCAAAAAGCT TGGCAATCCG GTACTGTTGG
      781 TAAAGCCACC ATGGCTTCCA AGGTGTACGA CCCCGAGCAA CGCAAACGCA TGATCACTGG
      841 GCCTCAGTGG TGGGCTCGCT GCAAGCAAAT GAACGTGCTG GACTCCTTCA TCAACTACTA
      901 TGATTCCGAG AAGCACGCCG AGAACGCCGT GATTTTTCTG CATGGTAACG CTGCCTCCAG
      961 CTACCTGTGG AGGCACGTCG TGCCTCACAT CGAGCCCGTG GCTAGATGCA TCATCCCTGA
     1021 TCTGATCGGA ATGGGTAAGT CCGGCAAGAG CGGGAATGGC TCATATCGCC TCCTGGATCA
     1081 CTACAAGTAC CTCACCGCTT GGTTCGAGCT GCTGAACCTT CCAAAGAAAA TCATCTTTGT
     1141 GGGCCACGAC TGGGGGGCTT GTCTGGCCTT TCACTACTCC TACGAGCACC AAGACAAGAT
     1201 CAAGGCCATC GTCCATGCTG AGAGTGTCGT GGACGTGATC GAGTCCTGGG ACGAGTGGCC
     1261 TGACATCGAG GAGGATATCG CCCTGATCAA GAGCGAAGAG GGCGAGAAAA TGGTGCTTGA
     1321 GAATAACTTC TTCGTCGAGA CCATGCTCCC AAGCAAGATC ATGCGGAAAC TGGAGCCTGA
     1381 GGAGTTCGCT GCCTACCTGG AGCCATTCAA GGAGAAGGGC GAGGTTAGAC GGCCTACCCT
     1441 CTCCTGGCCT CGCGAGATCC CTCTCGTTAA GGGAGGCAAG CCCGACGTCG TCCAGATTGT
     1501 CCGCAACTAC AACGCCTACC TTCGGGCCAG CGACGATCTG CCTAAGATGT TCATCGAGTC
     1561 CGACCCTGGG TTCTTTTCCA ACGCTATTGT CGAGGGAGCT AAGAAGTTCC CTAACACCGA
     1621 GTTCGTGAAG GTGAAGGGCC TCCACTTCAG CCAGGAGGAC GCTCCAGATG AAATGGGTAA
     1681 GTACATCAAG AGCTTCGTGG AGCGCGTGCT GAAGAACGAG CAGTAATTCT AGAGTCGGGG
     1741 CGGCCGGCCG CTTCGAGCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC
     1801 TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT
     1861 AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA
     1921 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAA
     1981 AATCGATAAG GATCCGTCGA CCGATGCCCT TGAGAGCCTT CAACCCAGTC AGCTCCTTCC
     2041 GGTGGGCGCG GGGCATGACT ATCGTCGCCG CACTTATGAC TGTCTTCTTT ATCATGCAAC
     2101 TCGTAGGACA GGTGCCGGCA GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG
     2161 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA
     2221 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
     2281 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
     2341 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
     2401 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
     2461 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT
     2521 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
     2581 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
     2641 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
     2701 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT
     2761 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC
     2821 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA
     2881 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
     2941 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
     3001 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
     3061 GACAGCGGCC GCAAATGCTA AACCACTGCA GTGGTTACCA GTGCTTGATC AGTGAGGCAC
     3121 CGATCTCAGC GATCTGCCTA TTTCGTTCGT CCATAGTGGC CTGACTCCCC GTCGTGTAGA
     3181 TCACTACGAT TCGTGAGGGC TTACCATCAG GCCCCAGCGC AGCAATGATG CCGCGAGAGC
     3241 CGCGTTCACC GGCCCCCGAT TTGTCAGCAA TGAACCAGCC AGCAGGGAGG GCCGAGCGAA
     3301 GAAGTGGTCC TGCTACTTTG TCCGCCTCCA TCCAGTCTAT GAGCTGCTGT CGTGATGCTA
     3361 GAGTAAGAAG TTCGCCAGTG AGTAGTTTCC GAAGAGTTGT GGCCATTGCT ACTGGCATCG
     3421 TGGTATCACG CTCGTCGTTC GGTATGGCTT CGTTCAACTC TGGTTCCCAG CGGTCAAGCC
     3481 GGGTCACATG ATCACCCATA TTATGAAGAA ATGCAGTCAG CTCCTTAGGG CCTCCGATCG
     3541 TTGTCAGAAG TAAGTTGGCC GCGGTGTTGT CGCTCATGGT AATGGCAGCA CTACACAATT
     3601 CTCTTACCGT CATGCCATCC GTAAGATGCT TTTCCGTGAC CGGCGAGTAC TCAACCAAGT
     3661 CGTTTTGTGA GTAGTGTATA CGGCGACCAA GCTGCTCTTG CCCGGCGTCT ATACGGGACA
     3721 ACACCGCGCC ACATAGCAGT ACTTTGAAAG TGCTCATCAT CGGGAATCGT TCTTCGGGGC
     3781 GGAAAGACTC AAGGATCTTG CCGCTATTGA GATCCAGTTC GATATAGCCC ACTCTTGCAC
     3841 CCAGTTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC GGGGTGTGCA AAAACAGGCA
     3901 AGCAAAATGC CGCAAAGAAG GGAATGAGTG CGACACGAAA ATGTTGGATG CTCATACTCG
     3961 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTACTA GTACGTCTCT CAAGGATAAG
     4021 TAAGTAATAT TAAGGTACGG GAGGTATTGG ACAGGCCGCA ATAAAATATC TTTATTTTCA
     4081 TTACATCTGT GTGTTGGTTT TTTGTGTGAA TCGATAGTAC TAACATACGC TCTCCATCAA
     4141 AACAAAACGA AACAAAACAA ACTAGCAAAA TAGGCTGTCC CCAGTGCAAG TGCAGGTGCC
     4201 AGAACATTTC TCT
//