LOCUS       pTYC1_pGYL1+5898        5203 bp ds-DNA     circular     07-AUG-2023
DEFINITION  .
KEYWORDS    "accession:AY738222" "creator:Promega" "marker:AmpR"
            "organism:Mammalian Cells"
COMMENT     Promoterless vector for measuring the activity of promoter and
            enhancer sequences with a luciferase assay.
FEATURES             Location/Qualifiers
     misc_feature    20..669
                     /label="all_tcpo_peak_5898"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    670..694
                     /label="HiFi_homology arm2"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    708..1025
                     /label="minimal albpro_318bp"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1046..1065)
                     /label="FF luc 5' reverse"
                     /note="sequence: TCCATGGTGGCTTTACCAAC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1061..2713
                     /label="firefly luciferase"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1295..1314
                     /label="ON8047"
                     /note="sequence: GTGGTGTGCAGCGAGAATAG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(1374..1395)
                     /label="ON8048"
                     /note="sequence: CGCTCGTTGTAGATGTCGTTAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2575..2594
                     /label="ON8045"
                     /note="sequence: GGTTACAACCGCCAAGAAGC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2617..2636)
                     /label="ON8046"
                     /note="sequence: GTCCTTTAGGCACCTCGTCC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     polyA_signal    2748..2939
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     polyA_signal    2757..2878
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     rep_origin      complement(3297..3885)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          3746..3765
                     /label="ON7888_AAVtiter_ORI_F"
                     /note="sequence: TTGGTATCTGCGCTCTGCTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(3830..3849)
                     /label="ON7889_AAVTiter_ORI_R"
                     /note="sequence: GCAAACAAAAAAACCACCGC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             complement(4085..4876)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(4877..4945)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     polyA_signal    5050..5098
                     /label="poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    5112..5203
                     /label="pause site"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          5152..5171
                     /label="RV3primer"
                     /note="sequence: CTAGCAAAATAGGCTGTCCC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    5202..19
                     /label="HiFi_homology arm1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 GGCCTAACTG GCCGGTACCG GCTGTTCTCA TCATGCGCAT GTAGCTGAGG AAGACCAGCT
       61 CCCCTAGCCT CCACTATGCA GTACCAGATT GACTGGAGTG TGCTTGTTGG AAGGTTTTAC
      121 TTTGGCAGGT ACAAATTCAC AGGCCTGGGT GTGTGGACAA CAGCCAACTA TGACCTACAG
      181 CACCGTGACC CCCTGCCCAC TGCCCTTCAC CTCGCCTGGG CCATTGATCA TGGTAACATG
      241 GACCAAGAGG GCAGCGGGGT GCAGGGGTCC CTCCAGTGCA GAGCATGCTG GCCTGACTCC
      301 CGAGCACCAC CTGCTGTTGC CTGGGGCAAA CTCTGGGTTT GGAGGACTCC CAGGTAAGAG
      361 AAACCCTGTG ACCCCAACAA CCCTGTAGGT GAAGAGGAGG TTCACAAGGT GAACTCTGGT
      421 GACTTCACAG CACCAAGAGC TTGATGGGTC AGAGCCACCT CCAACCAAGT CATTGTCCTC
      481 ACCAGAGACC TTCTGTAACA GTGCCTGCAG CTCAACTGAC ACAGCTTGCT CATAGTCTAG
      541 TTGGTAAGGT GCTTGTAGCT CAGCCAATAC AGTACTTGTA GCCCAGTGAG TAAGGTGCTT
      601 GCAGCTCAGT TGGTAGAGTG CTTGTCAAGC ACACAGGAGG CCCATCAGAT CACCCCATAA
      661 GCAAGGTAGC TCGAGGATAT CAAGATCTGG CCTCGGCGGC CAAGCTTTGC TCAAATGGGA
      721 GACAAAGAGA TTAAGCTCTT ATGTAAAATT TGCTGTTTTA CATAACTTTA ATGAATGGAC
      781 AAAGTCTTGT GCATGGGGGT GGGGGTGGGG TTAGAGGGGA ACAGCTCCAG ATGGCAAACA
      841 TACGCAAGGG ATTTAGTCAA ACAACTTTTT GGCAAAGATG GTATGATTTT GTAATGGGGT
      901 AGGAACCAAT GAAATGCGAG GTAAGTATGG TTAATAATCT ACAGTTATTG GTTAAAGAAG
      961 TATATTAGAG CGAGTCTTTC TGCACACAGA TCACCTTCCT ATCAACCCCA CTAGCCTCTG
     1021 GCAAAAAGCT TGGCAATCCG GTACTGTTGG TAAAGCCACC ATGGAAGATG CCAAAAACAT
     1081 TAAGAAGGGC CCAGCGCCAT TCTACCCACT CGAAGACGGG ACCGCCGGCG AGCAGCTGCA
     1141 CAAAGCCATG AAGCGCTACG CCCTGGTGCC CGGCACCATC GCCTTTACCG ACGCACATAT
     1201 CGAGGTGGAC ATTACCTACG CCGAGTACTT CGAGATGAGC GTTCGGCTGG CAGAAGCTAT
     1261 GAAGCGCTAT GGGCTGAATA CAAACCATCG GATCGTGGTG TGCAGCGAGA ATAGCTTGCA
     1321 GTTCTTCATG CCCGTGTTGG GTGCCCTGTT CATCGGTGTG GCTGTGGCCC CAGCTAACGA
     1381 CATCTACAAC GAGCGCGAGC TGCTGAACAG CATGGGCATC AGCCAGCCCA CCGTCGTATT
     1441 CGTGAGCAAG AAAGGGCTGC AAAAGATCCT CAACGTGCAA AAGAAGCTAC CGATCATACA
     1501 AAAGATCATC ATCATGGATA GCAAGACCGA CTACCAGGGC TTCCAAAGCA TGTACACCTT
     1561 CGTGACTTCC CATTTGCCAC CCGGCTTCAA CGAGTACGAC TTCGTGCCCG AGAGCTTCGA
     1621 CCGGGACAAA ACCATCGCCC TGATCATGAA CAGTAGTGGC AGTACCGGAT TGCCCAAGGG
     1681 CGTAGCCCTA CCGCACCGCA CCGCTTGTGT CCGATTCAGT CATGCCCGCG ACCCCATCTT
     1741 CGGCAACCAG ATCATCCCCG ACACCGCTAT CCTCAGCGTG GTGCCATTTC ACCACGGCTT
     1801 CGGCATGTTC ACCACGCTGG GCTACTTGAT CTGCGGCTTT CGGGTCGTGC TCATGTACCG
     1861 CTTCGAGGAG GAGCTATTCT TGCGCAGCTT GCAAGACTAT AAGATTCAAT CTGCCCTGCT
     1921 GGTGCCCACA CTATTTAGCT TCTTCGCTAA GAGCACTCTC ATCGACAAGT ACGACCTAAG
     1981 CAACTTGCAC GAGATCGCCA GCGGCGGGGC GCCGCTCAGC AAGGAGGTAG GTGAGGCCGT
     2041 GGCCAAACGC TTCCACCTAC CAGGCATCCG CCAGGGCTAC GGCCTGACAG AAACAACCAG
     2101 CGCCATTCTG ATCACCCCCG AAGGGGACGA CAAGCCTGGC GCAGTAGGCA AGGTGGTGCC
     2161 CTTCTTCGAG GCTAAGGTGG TGGACTTGGA CACCGGTAAG ACACTGGGTG TGAACCAGCG
     2221 CGGCGAGCTG TGCGTCCGTG GCCCCATGAT CATGAGCGGC TACGTTAACA ACCCCGAGGC
     2281 TACAAACGCT CTCATCGACA AGGACGGCTG GCTGCACAGC GGCGACATCG CCTACTGGGA
     2341 CGAGGACGAG CACTTCTTCA TCGTGGACCG GCTGAAGAGC CTGATCAAAT ACAAGGGCTA
     2401 CCAGGTAGCC CCAGCCGAAC TGGAGAGCAT CCTGCTGCAA CACCCCAACA TCTTCGACGC
     2461 CGGGGTCGCC GGCCTGCCCG ACGACGATGC CGGCGAGCTG CCCGCCGCAG TCGTCGTGCT
     2521 GGAACACGGT AAAACCATGA CCGAGAAGGA GATCGTGGAC TATGTGGCCA GCCAGGTTAC
     2581 AACCGCCAAG AAGCTGCGCG GTGGTGTTGT GTTCGTGGAC GAGGTGCCTA AAGGACTGAC
     2641 CGGCAAGTTG GACGCCCGCA AGATCCGCGA GATTCTCATT AAGGCCAAGA AGGGCGGCAA
     2701 GATCGCCGTG TAATAATTCT AGAGTCGGGG CGGCCGGCCG CTTCGAGCAG ACATGATAAG
     2761 ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG
     2821 TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA
     2881 CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA
     2941 AAGCAAGTAA AACCTCTACA AATGTGGTAA AATCGATAAG GATCCGTCGA CCGATGCCCT
     3001 TGAGAGCCTT CAACCCAGTC AGCTCCTTCC GGTGGGCGCG GGGCATGACT ATCGTCGCCG
     3061 CACTTATGAC TGTCTTCTTT ATCATGCAAC TCGTAGGACA GGTGCCGGCA GCGCTCTTCC
     3121 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT
     3181 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG
     3241 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC
     3301 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA
     3361 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
     3421 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
     3481 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
     3541 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
     3601 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
     3661 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
     3721 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC
     3781 GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT
     3841 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC
     3901 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     3961 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     4021 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGCGGCC GCAAATGCTA AACCACTGCA
     4081 GTGGTTACCA GTGCTTGATC AGTGAGGCAC CGATCTCAGC GATCTGCCTA TTTCGTTCGT
     4141 CCATAGTGGC CTGACTCCCC GTCGTGTAGA TCACTACGAT TCGTGAGGGC TTACCATCAG
     4201 GCCCCAGCGC AGCAATGATG CCGCGAGAGC CGCGTTCACC GGCCCCCGAT TTGTCAGCAA
     4261 TGAACCAGCC AGCAGGGAGG GCCGAGCGAA GAAGTGGTCC TGCTACTTTG TCCGCCTCCA
     4321 TCCAGTCTAT GAGCTGCTGT CGTGATGCTA GAGTAAGAAG TTCGCCAGTG AGTAGTTTCC
     4381 GAAGAGTTGT GGCCATTGCT ACTGGCATCG TGGTATCACG CTCGTCGTTC GGTATGGCTT
     4441 CGTTCAACTC TGGTTCCCAG CGGTCAAGCC GGGTCACATG ATCACCCATA TTATGAAGAA
     4501 ATGCAGTCAG CTCCTTAGGG CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCGGTGTTGT
     4561 CGCTCATGGT AATGGCAGCA CTACACAATT CTCTTACCGT CATGCCATCC GTAAGATGCT
     4621 TTTCCGTGAC CGGCGAGTAC TCAACCAAGT CGTTTTGTGA GTAGTGTATA CGGCGACCAA
     4681 GCTGCTCTTG CCCGGCGTCT ATACGGGACA ACACCGCGCC ACATAGCAGT ACTTTGAAAG
     4741 TGCTCATCAT CGGGAATCGT TCTTCGGGGC GGAAAGACTC AAGGATCTTG CCGCTATTGA
     4801 GATCCAGTTC GATATAGCCC ACTCTTGCAC CCAGTTGATC TTCAGCATCT TTTACTTTCA
     4861 CCAGCGTTTC GGGGTGTGCA AAAACAGGCA AGCAAAATGC CGCAAAGAAG GGAATGAGTG
     4921 CGACACGAAA ATGTTGGATG CTCATACTCG TCCTTTTTCA ATATTATTGA AGCATTTATC
     4981 AGGGTTACTA GTACGTCTCT CAAGGATAAG TAAGTAATAT TAAGGTACGG GAGGTATTGG
     5041 ACAGGCCGCA ATAAAATATC TTTATTTTCA TTACATCTGT GTGTTGGTTT TTTGTGTGAA
     5101 TCGATAGTAC TAACATACGC TCTCCATCAA AACAAAACGA AACAAAACAA ACTAGCAAAA
     5161 TAGGCTGTCC CCAGTGCAAG TGCAGGTGCC AGAACATTTC TCT
//