LOCUS       pTYC10_hSTARR_seq_albpr 4549 bp ds-DNA     circular     07-AUG-2023
DEFINITION  .
KEYWORDS    "marker:AmpR, CmR" "ref:pmid\:29256496"
COMMENT     Vector to measure enhancer activity of candidates from a DNA
            library by STARR-seq.. <small>[1] Resolving systematic errors in
            widely used enhancer activity assays in human cells. Nat Methods.
            2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534. Muerdter F,
            Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, Pagani M,
            Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold CD, Stark A
            (pmid:29256496)</small>
FEATURES             Location/Qualifiers
     primer          15..36
                     /label="hSTARR-seq_SCP1 vector 15-36 FWD"
                     /note="sequence: caagtgcaggtgccagaacatt"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          47..66
                     /label="homology arm of ON8196 "
                     /note="sequence: aactggccggtacctgagct"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    68..385
                     /label="minimal albpro_318bp"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(387..406)
                     /label="homology arm of ON8197"
                     /note="sequence: cagtgcctcacgaccgggcc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     intron          412..544
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     primer          455..474
                     /label="ON8381-plasmid library forward"
                     /note="sequence: aactgggcttgtcgagacag"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(527..548)
                     /label="hSTARR-seq_SCP1 vector 296-317 REV"
                     /note="sequence: acacctgtggagagaaaggcaa"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          577..596
                     /label="ON8198-hSTARR_seq_sequencing primer-F"
                     /note="sequence: aaaggagaagaactcttcac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    633..647
                     /label="homology arm1-AgeI"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     promoter        679..684
                     /label="lac UV5 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        679..709
                     /label="lac UV5 promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        685..702
                     /label="lac UV5 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        703..709
                     /label="lac UV5 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             763..1419
                     /label="CmR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             763..1422
                     /label="CmR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1764..2069
                     /label="ccdB"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             1857..2066
                     /label="ccdB"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2111..2125
                     /label="homology arm-SalI"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(2128..2148)
                     /label="ON8382 plasmid library reverse"
                     /note="sequence: tatcatgtctgctcgaagcgg"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     polyA_signal    2138..2329
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     polyA_signal    2147..2268
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2212..2231)
                     /label="ON8199-hSTARR_seq_revSeqprimer/SV40pA-R"
                     /note="sequence: gcaatagcatcacaaatttc"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(2666..3348)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      complement(2684..3272)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          3133..3152
                     /label="ON7888_AAVtiter_ORI_F"
                     /note="sequence: TTGGTATCTGCGCTCTGCTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(3472..4263)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(4264..4332)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     polyA_signal    4437..4485
                     /label="poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4499..41
                     /label="pause site"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 ggctgtcccc agtgcaagtg caggtgccag aacatttctc tggcctaact ggccggtacc
       61 tgagctcTGC TCAAATGGGA GACAAAGAGA TTAAGCTCTT ATGTAAAATT TGCTGTTTTA
      121 CATAACTTTA ATGAATGGAC AAAGTCTTGT GCATGGGGGT GGGGGTGGGG TTAGAGGGGA
      181 ACAGCTCCAG ATGGCAAACA TACGCAAGGG ATTTAGTCAA ACAACTTTTT GGCAAAGATG
      241 GTATGATTTT GTAATGGGGT AGGAACCAAT GAAATGCGAG GTAAGTATGG TTAATAATCT
      301 ACAGTTATTG GTTAAAGAAG TATATTAGAG CGAGTCTTTC TGCACACAGA TCACCTTCCT
      361 ATCAACCCCA CTAGCCTCTG GCAAAgggcc cggtcgtgag gcactgggca ggtaagtatc
      421 aaggttacaa gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga
      481 ctcttgcgtt tctgataggc acctattggt cttactgaca tccactttgc ctttctctcc
      541 acaggtgtcc accctgcagg cttaagcatg gctagcaaag gagaagaact cttcactgga
      601 gttgtcccaa ttcttgttga attagattga tctagagcat gcaccggtga tatcgcggcc
      661 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      721 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      781 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      841 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      901 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      961 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
     1021 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     1081 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     1141 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1201 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1261 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1321 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1381 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg atccggctta
     1441 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1501 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     1561 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1621 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1681 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1741 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1801 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1861 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1921 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1981 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     2041 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     2101 caggatatcg tcgacgaatt cggccggccg cttcgagcag acatgataag atacattgat
     2161 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2221 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     2281 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     2341 aacctctaca aatgtggtaa aatcgataag gatccgaccg atgcccttga gagccttcaa
     2401 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
     2461 cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca
     2521 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2581 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2641 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2701 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2761 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2821 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2881 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2941 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3001 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3061 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3121 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3181 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3241 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3301 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3361 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3421 atgagtaaac ttggtctgac agcggccgca aatgctaaac cactgcagtg gttaccagtg
     3481 cttgatcagt gaggcaccga tctcagcgat ctgcctattt cgttcgtcca tagtggcctg
     3541 actccccgtc gtgtagatca ctacgattcg tgagggctta ccatcaggcc ccagcgcagc
     3601 aatgatgccg cgagagccgc gttcaccggc ccccgatttg tcagcaatga accagccagc
     3661 agggagggcc gagcgaagaa gtggtcctgc tactttgtcc gcctccatcc agtctatgag
     3721 ctgctgtcgt gatgctagag taagaagttc gccagtgagt agtttccgaa gagttgtggc
     3781 cattgctact ggcatcgtgg tatcacgctc gtcgttcggt atggcttcgt tcaactctgg
     3841 ttcccagcgg tcaagccggg tcacatgatc acccatatta tgaagaaatg cagtcagctc
     3901 cttagggcct ccgatcgttg tcagaagtaa gttggccgcg gtgttgtcgc tcatggtaat
     3961 ggcagcacta cacaattctc ttaccgtcat gccatccgta agatgctttt ccgtgaccgg
     4021 cgagtactca accaagtcgt tttgtgagta gtgtatacgg cgaccaagct gctcttgccc
     4081 ggcgtctata cgggacaaca ccgcgccaca tagcagtact ttgaaagtgc tcatcatcgg
     4141 gaatcgttct tcggggcgga aagactcaag gatcttgccg ctattgagat ccagttcgat
     4201 atagcccact cttgcaccca gttgatcttc agcatctttt actttcacca gcgtttcggg
     4261 gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga atgagtgcga cacgaaaatg
     4321 ttggatgctc atactcgtcc tttttcaata ttattgaagc atttatcagg gttactagta
     4381 cgtctctcaa ggataagtaa gtaatattaa ggtacgggag gtattggaca ggccgcaata
     4441 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa
     4501 catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaata
//