LOCUS       pERASE                  4457 bp DNA     circular SYN 22-MAR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 22, 2024 from SnapGene Viewer 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(524..2200,4438..4457,1..523)
                     /mol_type="other DNA"
                     /note="This feature has 7 segments: 1: 524 .. 947 /
                     #ffffff 2: 948 .. 1114 / #ffffff 3: 1115 .. 1121 / #ffffff 
                     4: 1122 .. 1126 / #ffffff 5: 1127 .. 2170 / #ffffff 6: 2171
                     .. 2200 / #ffffff 7: 4438 .. 523 / #ffffff"
                     /organism="synthetic DNA construct"
     source          3332..4366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(4438..4457,1..947)
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      152..740
     primer_bind     894..911
                     /standard_name="L4440"
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        948..1114
                     /standard_name="Pveg"
                     /label=Pveg promoter
                     /note="/created_by=g_geneious"
                     /note="/modified_by=lautenschlaeger"
     promoter        1034..1039
                     /standard_name="-35"
                     /label=-35 promoter
                     /note="/created_by=g_geneious"
     promoter        1055..1063
                     /standard_name="-10"
                     /label=-10 promoter
                     /note="/created_by=g_geneious"
     misc_feature    1115..1121
                     /standard_name="RBS"
                     /label=nonstandard type: Ribosome Binding site
                     /note="/created_by=g_geneious"
     CDS             1127..2170
                     /codon_start=1
                     /standard_name="PheS*"
                     /label=PheS*
                     /translation="MDLQAQLEELKTKTLETLQSLTGNHTKELQDLRVAVLGKKGSLTD
                     LLKGLKDLSNDLRPVVGKQVNEVRDLLTKAFEEQAKIVEAAKIQAQLDAESIDVTLPGR
                     QMTLGHRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDMQDTF
                     YITEEILLRTHTSPVQARTLDQHDFSKGPLKMVSPGRVFRRDTDDATHSHQFHQIEGLV
                     VGKNISMGDLKGTLEMIIKKMFGDERSIRLRPSYFPFSEPSVEVDVSCFKCGGKGCNVC
                     KKTGWIEILGAGMVHPSVLEMSGVDAKEYSGFGFGLGQERIAMLRYGINDIRGFYQGDQ
                     RFSEQFN"
     terminator      2171..2200
                     /standard_name="tt3"
                     /label=tt3 terminator
                     /note="/vntifkey=43"
     -35_signal      2366..2371
                     /standard_name="Putative -35 ermB"
                     /label=Putative -35 ermB
     -10_signal      2390..2398
                     /standard_name="Putative -10 ermB"
                     /label=Putative -10 ermB
     RBS             2439..2445
                     /standard_name="ermB RBS"
                     /label=ermB RBS
     CDS             2454..3188
                     /standard_name="ermB"
                     /label=ermB
     protein_bind    3398..3419
                     /standard_name="CAP binding site"
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the
                     presenceof cAMP."
     promoter        3434..3464
                     /standard_name="lac promoter"
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3472..3488
                     /standard_name="lac operator binding site"
                     /label=lac operator binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3506..3517
                     /standard_name="RBS"
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3524..4201
                     /codon_start=1
                     /standard_name="mRFP1"
                     /product="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      4246..4317
                     /standard_name="rrnB T1 terminator"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4333..4360
                     /standard_name="T7Te terminator"
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(4404..4420)
                     /standard_name="M13r"
                     /label=M13r
                     /note="color: black; sequence: caggaaacagctatgac; added: 
                     2023-02-10"
ORIGIN
        1 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
       61 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
      121 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      181 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      241 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
      301 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
      361 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
      421 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
      481 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
      541 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      601 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      661 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      721 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
      781 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      841 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
      901 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcttt tttattttac
      961 ataatgatac ataatttacc gaaacttgcg gaacataatt gaggaatcat agaattttgt
     1021 caaaataatt ttattgacaa cgtcttatta acgttgatat aatttaaatt ttatttgaca
     1081 aaaatgggct cgtgttgtac aataaatgta gttaaaggag gataatatgg accttcaagc
     1141 tcaattggaa gaacttaaaa ctaaaacctt ggaaactctt caaagtctta ctggtaacca
     1201 tacaaaagaa ttgcaagatt tgcgtgttgc agttcttggt aagaagggtt cacttactga
     1261 tttgcttaaa ggtcttaagg atttgtcaaa cgatttgcgt cctgtggtag gcaaacaagt
     1321 aaacgaagtt cgtgacttat tgacaaaagc tttcgaagag caagctaaga tcgttgaagc
     1381 cgctaaaatt caagctcaat tggatgctga atcaatcgac gttactcttc ctggacgtca
     1441 aatgactctg gggcatcgtc acgttcttac tcaaacatca gaagaaatcg aagacatctt
     1501 ccttggtatg ggtttccaaa ttgtagatgg tttcgaagtt gaaaaagatt attataactt
     1561 cgaacgtatg aaccttccaa aagaccaccc tgctcgtgac atgcaagaca ctttctacat
     1621 tactgaagag atcttgttac gtactcacac ttcaccagta caagctcgta ctcttgatca
     1681 acacgacttc tctaagggcc ctttaaaaat ggtttcacca ggccgtgtat tccgccgcga
     1741 cactgacgac gctactcact cacaccaatt ccaccaaatc gaaggtcttg tagttggcaa
     1801 aaatatttca atgggtgact tgaaaggcac tttggaaatg atcattaaaa aaatgttcgg
     1861 tgacgaacgt agcatccgtc ttcgcccttc ttatttccct ttctcagaac catcagtaga
     1921 agttgacgta tcatgtttca aatgtggagg taaaggctgt aacgtttgta aaaaaactgg
     1981 ttggatcgaa atcttgggtg ctggtatggt tcacccaagt gttcttgaaa tgtcaggtgt
     2041 tgatgcaaaa gagtactcag gtttcggttt cggcttggga caagagcgta tcgctatgtt
     2101 gcgctacgga attaacgata ttcgtggctt ctaccaaggc gaccaacgtt tctcagaaca
     2161 atttaactaa aaaaaaggcc cacaaaagtg ggcctttttt aaacgaaatg atacaccaat
     2221 cagtgcaaaa aaagatataa tgggagataa gacggttcgt gttcgtgctg acttgcacca
     2281 tatcataaaa atcgaaacag caaagaatgg cggaaacgta aaagaagtta tggaaataag
     2341 acttagaagc aaacttaaga gtgtgttgat agtgcagtat cttaaaattt tgtataatag
     2401 gaattgaagt taaattagat gctaaaaatt tgtaattaag aaggagtgat tacatgaaca
     2461 aaaatataaa atattctcaa aactttttaa cgagtgaaaa agtactcaac caaataataa
     2521 aacaattgaa tttaaaagaa accgataccg tttacgaaat tggaacaggt aaagggcatt
     2581 taacgacgaa actggctaaa ataagtaaac aggtaacgtc tattgaatta gacagtcatc
     2641 tattcaactt atcgtcagaa aaattaaaac tgaatactcg tgtcacttta attcaccaag
     2701 atattctaca gtttcaattc cctaacaaac agaggtataa aattgttggg agtattcctt
     2761 accatttaag cacacaaatt attaaaaaag tggtttttga aagccatgcg tctgacatct
     2821 atctgattgt tgaagaagga ttctacaagc gtaccttgga tattcaccga acactagggt
     2881 tgctcttgca cactcaagtc tcgattcagc aattgcttaa gctgccagcg gaatgctttc
     2941 atcctaaacc aaaagtaaac agtgtcttaa taaaacttac ccgccatacc acagatgttc
     3001 cagataaata ttggaagcta tatacgtact ttgtttcaaa atgggtcaat cgagaatatc
     3061 gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa acacgccaaa gtaaacaatt
     3121 taagtaccgt tacttatgag caagtattgt ctatttttaa tagttatcta ttatttaacg
     3181 ggaggaaata attctatgag tcgcttttgt aaatttggaa agttacacgt tactaaaggg
     3241 aatgtagata aattattagg taaatctaaa acgtaaaacg acggccagtc agcgagacca
     3301 gctgaattca aaaaaaatct agatggccgg cgattcatta atgcagctgg cacgacaggt
     3361 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     3421 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     3481 gataacaatt tcacacatac tagagaaaga ggagaaatac tagatggctt cctccgaaga
     3541 cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga
     3601 gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact
     3661 gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca
     3721 gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc
     3781 cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac
     3841 cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg
     3901 taccaacttc ccgtccgatg gtccggttat gcagaaaaaa accatgggtt gggaagcttc
     3961 caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa
     4021 actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc
     4081 ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga
     4141 agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta
     4201 ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag
     4261 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     4321 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttataaccg gttaatacta
     4381 gtactgcagt gaaggtctca tgtgtcatag ctgtttcctg ctgtcagacc aagtttactt
     4441 cccgcgaaat taatacg
//
