LOCUS       2xUAS-1xhs-attB_       17094 bp DNA     circular SYN 24-APR-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17094)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Apr 24, 2024 from SnapGene Viewer 7.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        623..642
                     /label=T7 promoter
                     /note="T7 promoter"
                     /note="color: #ffc0cb"
     misc_feature    667..4802
                     /label=white dominant eye marker
                     /note="white dominant eye marker"
                     /note="color: #ff2430"
     misc_feature    2277..2297
                     /label=TRIP w hairpin
                     /note="color: #a6acb3"
     primer_bind     3876..3894
                     /label=MCS1Removal_PmlI_F
                     /note="color: black; sequence: aacaccacgtgccccagtt"
     primer_bind     4779..4799
                     /label=white_seqF
                     /note="color: black; sequence: atgcttggatttcactggaac; 
                     added: 2019-03-20"
     primer_bind     complement(4785..4803)
                     /label=MCS1Removal_PmeI_AvrII_AgeI_R
                     /note="color: black; sequence: 
                     atgcaACCGGTagtcCCTAGGagtcGTTTAAACctagttccagtgaaatcc"
     primer_bind     complement(4785..4802)
                     /label=MCS1Removal_AgeI_R
                     /note="color: black; sequence: 
                     atgcaACCGGTctagttccagtgaaatcc"
     misc_feature    4809..5172
                     /label=5xUAS-hsp70 basal promoter
                     /label=5xUAS-hsp70(2)
                     /note="5xUAS-hsp70"
                     /note="color: #7cf2ff"
     misc_feature    4824..4842
                     /label=UAS repeat
                     /label=UAS repeat(4)
                     /note="color: #a5c1ff"
     misc_feature    4843..4861
                     /label=UAS repeat
                     /label=UAS repeat(7)
                     /note="color: #4dffa4"
     misc_feature    4862..4880
                     /label=UAS repeat
                     /label=UAS repeat(8)
                     /note="color: #ff5ea9"
     misc_feature    4881..4899
                     /label=UAS repeat
                     /label=UAS repeat(10)
                     /note="color: #ff6cf3"
     misc_feature    4900..4916
                     /label=partial UAS repeat
                     /label=partial UAS repeat(2)
                     /note="color: #b33f8f"
     primer_bind     5055..5077
                     /label=pUAST F2
                     /note="color: black; sequence: CAGTAAAGTGCAAGTTAAAGTGA"
     primer_bind     5092..5113
                     /label=pV20 F
                     /note="color: black; sequence: ACCAGCAACCAAGTAAATCAAC"
     primer_bind     5112..5131
                     /label=Brainbow_FseI_F
                     /note="color: black; sequence: 
                     atgctGGCCGGCCactgcaactactgaaatctg; added: 2019-03-20"
     primer_bind     complement(5139..5161)
                     /label=hsp_AscI_R
                     /note="color: black; sequence: 
                     tgcatGGCGCGCCctcttcttgtattcaataattac"
     misc_feature    5173..5194
                     /label=MCS1
                     /label=multiple cloning site(2)
                     /note="multiple cloning site"
                     /note="color: #ff99cc"
     primer_bind     5186..5214
                     /label=Brainbow_FseI_F
                     /note="color: black; sequence: 
                     atgctGGCCGGCCactgcaactactgaaatctg; added: 2019-03-20"
     misc_feature    5195..9458
                     /label=dBrainbow
                     /note="color: #cc99ff"
     primer_bind     complement(5222..5244)
                     /label=hsp_AscI_R
                     /note="color: black; sequence: 
                     tgcatGGCGCGCCctcttcttgtattcaataattac"
     protein_bind    complement(5261..5294)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
                     /note="color: #31849b; direction: LEFT"
     protein_bind    complement(5316..5349)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
                     /note="color: #31849b; direction: LEFT"
     protein_bind    complement(5371..5404)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: LEFT"
     misc_feature    5408..6988
                     /label=spacer with poly(A) signal
                     /note="color: #666699"
     primer_bind     5445..5462
                     /label=EcoRI-m71-stop-F
                     /note="color: black; sequence: 
                     gcctaGAATTCgaagtttctatactttctagagaatagaaacttcgcccttcggccgcc
                     a; added: 2019-05-21"
     primer_bind     complement(5484..5501)
                     /label=pUAST-R
                     /note="color: black; sequence: cacaccacagaagtaagg; added: 
                     2019-03-20"
     intron          5544..5609
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /note="color: #------"
     primer_bind     complement(5613..5635)
                     /label=pUAST R2
                     /note="color: black; sequence: CATTCATCAGTTCCATAGGTTGG; 
                     added: 2019-03-20"
     CDS             5740..5760
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     primer_bind     complement(5935..5958)
                     /label=univSV40seqR
                     /note="color: black; sequence: ACCTCTACAAATGTGGTATGGCTG; 
                     added: 2019-03-20"
     primer_bind     6029..6057
                     /label=univSV40seqF
                     /note="color: black; sequence: 
                     GTTAACTTGTTTATTGCAGCTTATAATGG; added: 2019-03-20"
     polyA_signal    6032..6166
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(6056..6081)
                     /label=SV40 R JB26
                     /note="color: black; sequence: GTGATGCTATTGCTTTATTTGTAACC; 
                     added: 2019-03-20"
     primer_bind     complement(6069..6088)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC; added:
                     2019-05-21"
     primer_bind     6123..6142
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC; added:
                     2019-05-21"
     primer_bind     complement(6478..6497)
                     /label=stopcasette-seqR2
                     /note="color: black; sequence: gaccccgttctttcaccacg; added:
                     2019-05-21"
     primer_bind     complement(6975..7031)
                     /label=NotI-m71-stop-R
                     /note="color: black; sequence: 
                     tgagcggccgcgaagtttctattctctagaaagtatagaaacttccgtcttgcagttca
                     t; added: 2019-05-21"
     protein_bind    complement(6990..7023)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
                     /note="color: #31849b; direction: LEFT"
     CDS             7035..7748
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /note="color: #05fd14"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             7749..7781
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /note="color: #cc99b2"
                     /translation="QPELAPEDPED"
     protein_bind    complement(7801..7834)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
                     /note="color: #31849b; direction: LEFT"
     primer_bind     7837..7855
                     /label=mCherry_F_NotI
                     /note="color: black; sequence: 
                     tgcatGCGGCCGCCCAAATGGTGAGCAAGGGCGA; added: 2019-05-21"
     primer_bind     7837..7855
                     /label=mCherry_F_FseI
                     /note="color: black; sequence: 
                     tgcatGGCCGGCCCCAAATGGTGAGCAAGGGCGA; added: 2019-05-21"
     CDS             7839..8546
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /note="color: #00ffff"
                     /translation="MVSKGEELTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     primer_bind     complement(8020..8039)
                     /label=mTFP1_seqR
                     /note="color: black; sequence: ggtcagaatgtcgtaggaga"
     primer_bind     complement(8527..8548)
                     /label=mCherry KpnI-R
                     /note="color: black; sequence: 
                     tgcagGGTACCTTACTTGTACAGCTCGTCCATGC; added: 2019-05-21"
     primer_bind     complement(8528..8548)
                     /label=mCherry_seq_R
                     /note="color: black; sequence: TTACTTGTACAGCTCGTCCATG; 
                     added: 2019-05-21"
     primer_bind     complement(8531..8548)
                     /label=mCherry_R_XbaI
                     /note="color: black; sequence: 
                     ctcgaTCTAGATTACTTGTACAGCTCGTCC; added: 2019-05-21"
     primer_bind     complement(8531..8548)
                     /label=mCherry_R_PacI
                     /note="color: black; sequence: 
                     ctcgaTTAATTAATTACTTGTACAGCTCGTCC; added: 2019-05-21"
     misc_feature    8547..8639
                     /label=3x HA tag
                     /note="color: #ffffff"
     CDS             8547..8573
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             8577..8603
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             8607..8633
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     protein_bind    complement(8659..8692)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: LEFT"
     CDS             8697..9398
                     /codon_start=1
                     /label=mKO2
                     /note="color: #ff0000"
                     /translation="MIPVGVSKGEESVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYE
                     GHQEMTLRVTMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERS
                     LEFEDGGSASVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDG
                     VLKGDVTMYLKLEGGGNHKCQMKTTYKAAKGILEMPGDHYIGHRLVRKTEGNITEQVED
                     AVAHSGMDELYK"
     primer_bind     complement(8829..8847)
                     /label=mKO2_seq_R
                     /note="color: black; sequence: tcatctcctggtgtccttc"
     primer_bind     9207..9225
                     /label=mKO2_seq_F
                     /note="color: black; sequence: atgtacctgaagctggagg"
     primer_bind     complement(9379..9398)
                     /label=mCherry KpnI-R
                     /note="color: black; sequence: 
                     tgcagGGTACCTTACTTGTACAGCTCGTCCATGC; added: 2019-05-21"
     primer_bind     complement(9380..9398)
                     /label=mCherry_seq_R
                     /note="color: black; sequence: TTACTTGTACAGCTCGTCCATG; 
                     added: 2019-05-21"
     primer_bind     complement(9383..9398)
                     /label=mCherry_R_XbaI
                     /note="color: black; sequence: 
                     ctcgaTCTAGATTACTTGTACAGCTCGTCC; added: 2019-05-21"
     primer_bind     complement(9383..9398)
                     /label=mCherry_R_PacI
                     /note="color: black; sequence: 
                     ctcgaTTAATTAATTACTTGTACAGCTCGTCC; added: 2019-05-21"
     CDS             9414..9458
                     /codon_start=1
                     /label=V5 tag
                     /note="color: #993366"
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(9443..9469)
                     /label=Brainbow_PacI_R
                     /note="color: black; sequence: 
                     tcgagTTAATTAAgtcaggtgctatccagg"
     misc_feature    9460..9483
                     /label=MCS1
                     /label=multiple cloning site(2)
                     /note="multiple cloning site"
                     /note="color: #ff99cc"
     misc_feature    9484..10183
                     /label=SV40 poly(A)
                     /label=SV40 poly(A)(2)
                     /note="SV40 poly(A)"
                     /note="color: #7dff74"
     primer_bind     complement(9501..9518)
                     /label=pUAST-R
                     /note="color: black; sequence: cacaccacagaagtaagg; added: 
                     2019-03-20"
     primer_bind     complement(9630..9652)
                     /label=pUAST R2
                     /note="color: black; sequence: CATTCATCAGTTCCATAGGTTGG; 
                     added: 2019-03-20"
     primer_bind     complement(9951..9974)
                     /label=univSV40seqR
                     /note="color: black; sequence: ACCTCTACAAATGTGGTATGGCTG; 
                     added: 2019-03-20"
     primer_bind     10045..10073
                     /label=univSV40seqF
                     /note="color: black; sequence: 
                     GTTAACTTGTTTATTGCAGCTTATAATGG; added: 2019-03-20"
     primer_bind     complement(10072..10097)
                     /label=SV40 R JB26
                     /note="color: black; sequence: GTGATGCTATTGCTTTATTTGTAACC; 
                     added: 2019-03-20"
     primer_bind     complement(10085..10104)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC; added:
                     2019-05-21"
     primer_bind     10139..10158
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC; added:
                     2019-05-21"
     primer_bind     10184..10208
                     /label=hsp_HindIII_F
                     /note="color: black; sequence: 
                     tcgagAAGCTTctagaatcccaaaacaaac"
     misc_feature    10190..10635
                     /label=heat-shock inducible Hsp70 promoter
                     /note="color: #99cc00"
     primer_bind     10529..10551
                     /label=pUAST F2
                     /note="color: black; sequence: CAGTAAAGTGCAAGTTAAAGTGA"
     primer_bind     10566..10587
                     /label=pV20 F
                     /note="color: black; sequence: ACCAGCAACCAAGTAAATCAAC"
     primer_bind     10586..10605
                     /label=Brainbow_FseI_F
                     /note="color: black; sequence: 
                     atgctGGCCGGCCactgcaactactgaaatctg; added: 2019-03-20"
     primer_bind     complement(10613..10645)
                     /label=hsp_AscI_R
                     /note="color: black; sequence: 
                     tgcatGGCGCGCCctcttcttgtattcaataattac"
     primer_bind     10648..10703
                     /label=Fragment.FOR-BsiWI
                     /note="color: black; sequence: 
                     aatcgCGTACGaaacCATTATCTGagtgttaaatggtaggcatcacacacgattaacaa
                     c; added: 2019-02-18"
     misc_feature    10649..10654
                     /label=MCS2
                     /note="color: #ff99cc"
     primer_bind     10659..10703
                     /label=Fragment.FOR
                     /note="color: black; sequence: 
                     CATTATCTGagtgttaaatggtaggcatcacacacgattaacaac"
     misc_feature    10676..12752
                     /label=CreEBD w/ intron
                     /note="color: #a6acb3"
     CDS             10676..10678
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre start codon
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /translation="M"
     primer_bind     complement(10678..10698)
                     /label=pV2022R
                     /note="color: black; sequence: TAATCGTGTGTGATGCCTACC"
     intron          10679..10841
                     /label=ftz intron
                     /note="intron"
                     /note="color: #ffff00"
     primer_bind     complement(10813..10861)
                     /label=Fragment.REV
                     /note="color: black; sequence: 
                     tgtacggtcagtaaattggactgtaagcataagcaaagaaaaaatgggt"
     primer_bind     10822..10867
                     /label=Vector.FOR
                     /note="color: black; sequence: 
                     ttctttgcttatgcttacagtccaatttactgaccgtacaccaaaa"
     CDS             10841..11866
                     /codon_start=2
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     G"
     primer_bind     11819..11839
                     /label=Cre-seqF
                     /note="color: black; sequence: cctggatagtgaaacaggggc; 
                     added: 2019-03-20"
     primer_bind     complement(11848..11874)
                     /label=pRH200-R
                     /note="color: black; sequence: 
                     CGTTAGCGGCCGCTCGCCATCTTCCAGCAGGC"
     primer_bind     11867..11894
                     /label=EBD304
                     /note="color: black; sequence: 
                     CGCTCGCGGCCGCACAGCCTGGCCTTGTCCCTG"
     CDS             11875..12752
                     /codon_start=3
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /note="color: #993366"
                     /translation="SLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNL
                     ADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNL
                     LLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLK
                     SLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLY
                     SMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYIT
                     GEAEGFPATV"
     primer_bind     complement(12735..12760)
                     /label=CreEBD_PmeI_R
                     /note="color: black; sequence: 
                     tcgatGTTTAAACtcagactgtggcagggaa"
     misc_feature    12753..12760
                     /label=MCS2
                     /note="color: #ff99cc"
     primer_bind     12754..12770
                     /label=PmeI removal F
                     /note="color: black; sequence: 
                     tgactGCGATCGCTTTAAACctagaggatc"
     misc_feature    12766..13465
                     /label=SV40 poly(A)
                     /label=SV40 poly(A)(1)
                     /note="SV40 poly(A)"
                     /note="color: #7dff74"
     primer_bind     complement(12783..12800)
                     /label=pUAST-R
                     /note="color: black; sequence: cacaccacagaagtaagg; added: 
                     2019-03-20"
     primer_bind     complement(12912..12934)
                     /label=pUAST R2
                     /note="color: black; sequence: CATTCATCAGTTCCATAGGTTGG; 
                     added: 2019-03-20"
     primer_bind     complement(13233..13256)
                     /label=univSV40seqR
                     /note="color: black; sequence: ACCTCTACAAATGTGGTATGGCTG; 
                     added: 2019-03-20"
     primer_bind     13327..13355
                     /label=univSV40seqF
                     /note="color: black; sequence: 
                     GTTAACTTGTTTATTGCAGCTTATAATGG; added: 2019-03-20"
     primer_bind     complement(13354..13379)
                     /label=SV40 R JB26
                     /note="color: black; sequence: GTGATGCTATTGCTTTATTTGTAACC; 
                     added: 2019-03-20"
     primer_bind     complement(13367..13386)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC; added:
                     2019-05-21"
     primer_bind     13421..13440
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC; added:
                     2019-05-21"
     primer_bind     13457..13477
                     /label=stopcasette-seqF
                     /note="color: black; sequence: tctggatccGCATGCctgcag; 
                     added: 2019-05-21"
     primer_bind     complement(13457..13477)
                     /label=PmeI removal R
                     /note="color: black; sequence: ctgcagGCATGCggatccaga"
     misc_feature    13472..13835
                     /label=5xUAS-hsp70 basal promoter
                     /label=5xUAS-hsp70(1)
                     /note="5xUAS-hsp70"
                     /note="color: #7cf2ff"
     misc_feature    13487..13505
                     /label=UAS repeat
                     /label=UAS repeat(2)
                     /note="color: #a5c1ff"
     misc_feature    13506..13524
                     /label=UAS repeat
                     /label=UAS repeat(9)
                     /note="color: #4dffa4"
     misc_feature    13525..13543
                     /label=UAS repeat
                     /label=UAS repeat(6)
                     /note="color: #ff5ea9"
     misc_feature    13544..13562
                     /label=UAS repeat
                     /label=UAS repeat(11)
                     /note="color: #ff6cf3"
     misc_feature    13563..13579
                     /label=partial UAS repeat
                     /label=partial UAS repeat(1)
                     /note="color: #b33f8f"
     primer_bind     13718..13740
                     /label=pUAST F2
                     /note="color: black; sequence: CAGTAAAGTGCAAGTTAAAGTGA"
     primer_bind     13755..13776
                     /label=pV20 F
                     /note="color: black; sequence: ACCAGCAACCAAGTAAATCAAC"
     primer_bind     13775..13794
                     /label=Brainbow_FseI_F
                     /note="color: black; sequence: 
                     atgctGGCCGGCCactgcaactactgaaatctg; added: 2019-03-20"
     primer_bind     complement(13802..13824)
                     /label=hsp_AscI_R
                     /note="color: black; sequence: 
                     tgcatGGCGCGCCctcttcttgtattcaataattac"
     misc_feature    13836..13890
                     /label=MCS
                     /label=multiple cloning site(3)
                     /note="multiple cloning site"
                     /note="color: #ff99cc"
     misc_feature    13891..14590
                     /label=SV40 poly(A)
                     /note="SV40 poly(A)"
                     /note="color: #7dff74"
     primer_bind     complement(13908..13925)
                     /label=pUAST-R
                     /note="color: black; sequence: cacaccacagaagtaagg; added: 
                     2019-03-20"
     primer_bind     complement(14037..14059)
                     /label=pUAST R2
                     /note="color: black; sequence: CATTCATCAGTTCCATAGGTTGG; 
                     added: 2019-03-20"
     primer_bind     complement(14358..14381)
                     /label=univSV40seqR
                     /note="color: black; sequence: ACCTCTACAAATGTGGTATGGCTG; 
                     added: 2019-03-20"
     primer_bind     14452..14480
                     /label=univSV40seqF
                     /note="color: black; sequence: 
                     GTTAACTTGTTTATTGCAGCTTATAATGG; added: 2019-03-20"
     primer_bind     complement(14479..14504)
                     /label=SV40 R JB26
                     /note="color: black; sequence: GTGATGCTATTGCTTTATTTGTAACC; 
                     added: 2019-03-20"
     primer_bind     complement(14492..14511)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC; added:
                     2019-05-21"
     primer_bind     14546..14565
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC; added:
                     2019-05-21"
     misc_feature    14597..14881
                     /label=attB site
                     /note="attB site"
                     /note="color: #ffe84f"
     promoter        14905..14924
                     /label=T3 promoter
                     /note="T3 promoter"
                     /note="color: #ffc0cb"
     CDS             complement(join(16106..16900,16931..16966))
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /note="This feature has 2 segments:
                        1: 16106 .. 16900 / #ffc0cb
                        2: 16931 .. 16966 / #ffc0cb"
                     /db_xref="GI:124365493"
                     /protein_id="ABN09740.1"
                     /translation="MSIQHFRVALIPAHPETLVKVKDAEDQLGARVGYIELDLNSGKIL
                     ESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDG
                     MTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPN
                     DERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADK
                     SGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcct gcaggtcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
     4861 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagactc
     4921 tagcgagcgc cggagtataa atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa
     4981 caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca
     5041 agctaaacaa tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac
     5101 caagtaaatc aactgcaact actgaaatct gccaagaagt aattattgaa tacaagaaga
     5161 gaactctgaa tagggaattg ggaattggcc ggccactgca actactgaaa tctgccaaga
     5221 agtaattatt gaatacaaga agagaactct gaatagatct ataacttcgt ataaagtatc
     5281 ctatacgaag ttatgaggca gcgcggctat cgtggataac ttcgtataat gtgtactata
     5341 cgaagttatc tggccacgac gggcgttcct ataacttcgt ataatgtatg ctatacgaag
     5401 ttatgccctg aatgcggccg ccagtgtgat ggatatctgc agaattcgcc cttcggccgc
     5461 caaaatggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     5521 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     5581 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     5641 agtggtggaa tgcctttaat gaggaaaacc ctgttttgct cagaagaaat gccatctagt
     5701 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta
     5761 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
     5821 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac
     5881 aagaaaatta tggaaaaata tttgatgtat agtgccttga ctagagatca taatcagcca
     5941 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ctctgaacct
     6001 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     6061 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6121 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgcg tcgcgccact
     6181 tcaacgctcg atgggagcgt cattggtggg cggggtaacc gtcgaaatca gtgtttacgc
     6241 ttccaatcgc aacaaaaaat tcactgcaac actgaaaagc atacgaaaac gatgaagatt
     6301 gtacgaggaa ccataaagta ttttatccac aaagacacgt atagcagaaa agccaagtta
     6361 actcggcgat aagttgtgta cacaagaata aaatcggcca gattcagtgt tgtcagaaat
     6421 aagaaaaccc cactatgttt ttctttgcct tttctttctc ccagcgatca ttcatttcgt
     6481 ggtgaaagaa cggggtcatt gcacggagtt tcgactgcgg gaaagcagag ctgccgttca
     6541 cttcgtctat aattagcgct ttctattttc cccgattcgg gccgctgctg cgcttttccg
     6601 cctgctgttt gtggcaagtg tagcagcagg ctgtgcacgc agtgtgggca tgcacttggc
     6661 tttccaccgt tggtatcgat tctctgggac gatgagtcat tcctttcggg gccacagcat
     6721 aatcgttgcc agctcaccga aatggtgact tcatttctta actgccgtca agcatgcgat
     6781 tgtacataca tacatattta tatatgtaca tatttatgtg actatggtag gtcgatataa
     6841 tagcaatcaa cgcaagcaaa tgtgtcagtc ctgcttacag gaacgattct atttagtaat
     6901 tttcgttgta taaagtaatt atgtatgtat gtaagcccca taaatctgaa acaattaggc
     6961 aaaaccatgc gaggatgaac tgcaagacga taacttcgta taaagtatcc tatacgaagt
     7021 tatgcggccg caaaatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
     7081 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     7141 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     7201 catggccaac acttgtcact actttaactt atggtgttca atgcttttca agatacccag
     7261 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtccaggaaa
     7321 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
     7381 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
     7441 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
     7501 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg
     7561 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
     7621 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
     7681 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
     7741 tatacaaaca gcccgagctg gcccccgagg atcccgagga ttgacgtaag ctagctcgag
     7801 ataacttcgt ataatgtgta ctatacgaag ttatcaaaat ggtgagcaag ggcgaggagc
     7861 tcacaatggg cgtaatcaag cccgacatga agatcaagct gaagatggag ggcaacgtga
     7921 atggccacgc cttcgtgatc gagggcgagg gcgagggcaa gccctacgac ggcaccaaca
     7981 ccatcaacct ggaggtgaag gagggagccc ccctgccctt ctcctacgac attctgacca
     8041 ccgcgttcgc ctacggcaac agggccttca ccaagtaccc cgacgacatc cccaactact
     8101 tcaagcagtc cttccccgag ggctactctt gggagcgcac catgaccttc gaggacaagg
     8161 gcatcgtgaa ggtgaagtcc gacatctcca tggaggagga ctccttcatc tacgagatac
     8221 acctcaaggg cgagaacttc ccccccaacg gccccgtgat gcagaagaag accaccggct
     8281 gggacgcctc caccgagagg atgtacgtgc gcgacggcgt gctgaagggc gacgtcaagc
     8341 acaagctgct gctggagggc ggcggccacc accgcgttga cttcaagacc atctacaggg
     8401 ccaagaaggc ggtgaagctg cccgactatc actttgtgga ccaccgcatc gagatcctga
     8461 accacgacaa ggactacaac aaggtgaccg tttacgagag cgccgtggcc cgcaactcca
     8521 ccgacggcat ggacgagctg tacaagtacc catacgatgt tcctgactat gcgggctatc
     8581 cctatgacgt cccggactat gcaggatatc catatgacgt tccagattac gctggcggat
     8641 aacgtaagct agggtaccat aacttcgtat aatgtatgct atacgaagtt atcaaaatga
     8701 taccggtcgg agtaagcaaa ggtgaggaga gcgtgatcaa acccgagatg aagatgcgct
     8761 actatatgga cggcagcgtc aacggccacg agtttaccat cgagggcgag ggcacgggac
     8821 gcccgtacga aggacaccag gagatgaccc tgcgcgtgac catggccgag ggtggcccga
     8881 tgcccttcgc cttcgatctg gtgagccacg tgttctgcta cggtcaccgc gtcttcacca
     8941 aataccccga ggaaatcccc gattacttca agcaagcctt cccggagggc ctgtcgtggg
     9001 agcgcagcct ggagttcgag gatggcggaa gcgccagcgt gagcgcgcac atcagcctgc
     9061 ggggcaacac cttctaccat aagagcaagt tcaccggcgt gaatttccca gccgatggcc
     9121 ccatcatgca gaaccagagt gtggattggg agcccagcac cgagaagatc accgcaagcg
     9181 atggcgtgct gaagggcgat gttaccatgt acctgaagct ggagggaggc ggcaaccaca
     9241 agtgccagat gaaaaccacc tacaaggccg ccaaggggat cctggagatg cccggagatc
     9301 actacattgg ccaccgcctg gtccgcaaga cagaaggaaa catcaccgag caggtggagg
     9361 atgccgtggc gcattcgggc atggatgagc tgtacaaggg cgcgccagct aatggcaagc
     9421 ccatccccaa ccccctgctg ggcctggata gcacctgact taattaactg aagacgtcct
     9481 agaggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
     9541 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
     9601 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
     9661 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
     9721 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
     9781 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
     9841 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
     9901 aaattatgga aaaatatttg atgtatagtg ccttgactag agatcataat cagccatacc
     9961 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
    10021 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
    10081 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    10141 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccaagcttc tagaatccca
    10201 aaacaaactg gttattgtgg taggtcattt gtttggcaga aagaaaactc gagaaatttc
    10261 tctggccgtt attcgttatt ctctcttttc tttttgggtc tctccctctc tgcactaatg
    10321 ctctctcact ctgtcacaca gtaaacggca tactgctctc gttggttcga gagagcgcgc
    10381 ctcgaatgtt cgcgaaaaga gcgccggagt ataaatagag gcgcttcgtc tacggagcga
    10441 caattcaatt caaacaagca aagtgaacac gtcgctaagc gaaagctaag caaataaaca
    10501 agcgcagctg aacaagctaa acaatctgca gtaaagtgca agttaaagtg aatcaattaa
    10561 aagtaaccag caaccaagta aatcaactgc aactactgaa atctgccaag aagtaattat
    10621 tgaatacaag aagagggcgc gccatatgcg tacgaaacca ttatctgagt gttaaatggt
    10681 aggcatcaca cacgattaac aacccctaaa aatacacttt gaaaatattg aaaatatggg
    10741 tacctctagt ctcgagtttg tatacatttt tgatattttc aaacaatacg cagttataaa
    10801 actcattagc taacccattt tttctttgct tatgcttaca gtccaattta ctgaccgtac
    10861 accaaaattt gcctgcatta ccggtcgatg caacgagtga tgaggttcgc aagaacctga
    10921 tggacatgtt cagggatcgc caggcgtttt ctgagcatac ctggaaaatg cttctgtccg
    10981 tttgccggtc gtgggcggca tggtgcaagt tgaataaccg gaaatggttt cccgcagaac
    11041 ctgaagatgt tcgcgattat cttctatatc ttcaggcgcg cggtctggca gtaaaaacta
    11101 tccagcaaca tttgggccag ctaaacatgc ttcatcgtcg gtccgggctg ccacgaccaa
    11161 gtgacagcaa tgctgtttca ctggttatgc ggcggatccg aaaagaaaac gttgatgccg
    11221 gtgaacgtgc aaaacaggct ctagcgttcg aacgcactga tttcgaccag gttcgttcac
    11281 tcatggaaaa tagcgatcgc tgccaggata tacgtaatct ggcatttctg gggattgctt
    11341 ataacaccct gttacgtata gccgaaattg ccaggatcag ggttaaagat atctcacgta
    11401 ctgacggtgg gagaatgtta atccatattg gcagaacgaa aacgctggtt agcaccgcag
    11461 gtgtagagaa ggcacttagc ctgggggtaa ctaaactggt cgagcgatgg atttccgtct
    11521 ctggtgtagc tgatgatccg aataactacc tgttttgccg ggtcagaaaa aatggtgttg
    11581 ccgcgccatc tgccaccagc cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa
    11641 ctcatcgatt gatttacggc gctaaggatg actctggtca gagatacctg gcctggtctg
    11701 gacacagtgc ccgtgtcgga gccgcgcgag atatggcccg cgctggagtt tcaataccgg
    11761 agatcatgca agctggtggc tggaccaatg taaatattgt catgaactat atccgtaacc
    11821 tggatagtga aacaggggca atggtgcgcc tgctggaaga tggcgagcgg ccgcacagcc
    11881 tggccttgtc cctgacggcc gaccagatgg tcagtgcctt gttggatgct gagcccccca
    11941 tactctattc cgagtatgat cctaccagac ccttcagtga agcttcgatg atgggcttac
    12001 tgaccaacct ggcagacagg gagctggttc acatgatcaa ctgggcgaag agggtgccag
    12061 gctttgtgga tttgaccctc catgatcagg tccaccttct agaatgtgcc tggctagaga
    12121 tcctgatgat tggtctcgtc tggcgctcca tggagcaccc agggaagcta ctgtttgctc
    12181 ctaacttgct cttggacagg aaccagggaa aatgtgtaga gggcatggtg gagatcttcg
    12241 acatgctgct ggctacatca tctcggttcc gcatgatgaa tctgcaggga gaggagtttg
    12301 tgtgcctcaa atctattatt ttgcttaatt ctggagtgta cacatttctg tccagcaccc
    12361 tgaagtctct ggaagagaag gaccatatcc accgagtcct ggacaagatc acagacactt
    12421 tgatccacct gatggccaag gcaggcctga ccctgcagca gcagcaccag cggctggccc
    12481 agctcctcct catcctctcc cacatcaggc acatgagtaa caaaggcatg gagcatctgt
    12541 acagcatgaa gtgcaagaac gtggtgcccc tctatgacct gctgctggag atgctggacg
    12601 cccaccgcct acatgcgccc actagccgtg gaggggcatc cgtggaggag acggaccaaa
    12661 gccacttggc cactgcgggc tctacttcat cgcattcctt gcaaaagtat tacatcacgg
    12721 gggaggcaga gggtttccct gccacagtct gagtttaaac ctagaggatc tttgtgaagg
    12781 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
    12841 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
    12901 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
    12961 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
    13021 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
    13081 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
    13141 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
    13201 tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta
    13261 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
    13321 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    13381 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    13441 aatgtatctt atcatgtctg gatccgcatg cctgcaggtc ggagtactgt cctccgagcg
    13501 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
    13561 tactgtcctc cgagcggaga ctctagcgag cgccggagta taaatagagg cgcttcgtct
    13621 acggagcgac aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc
    13681 aaataaacaa gcgcagctga acaagctaaa caatctgcag taaagtgcaa gttaaagtga
    13741 atcaattaaa agtaaccagc aaccaagtaa atcaactgca actactgaaa tctgccaaga
    13801 agtaattatt gaatacaaga agagaactct gaatagggaa ttgggaattc gttaacagat
    13861 ctgcggccgc ggctcgaggg tacctctaga ggatctttgt gaaggaacct tacttctgtg
    13921 gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat
    13981 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa
    14041 cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt
    14101 gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc
    14161 ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt
    14221 ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa
    14281 aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atatttgatg tatagtgcct
    14341 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
    14401 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
    14461 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    14521 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    14581 gtctggatcc actagtgtcg acgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc
    14641 agggcgtgcc cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga
    14701 tgaacgggtc gaggtggcgg tagttgatcc cggcgaacgc gcggcgcacc gggaagccct
    14761 cgccctcgaa accgctgggc gcggtggtca cggtgagcac gggacgtgcg acggcgtcgg
    14821 cgggtgcgga tacgcggggc agcgtcagcg ggttctcgac ggtcacggcg ggcatgtcga
    14881 cactagttct agccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
    14941 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
    15001 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
    15061 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    15121 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
    15181 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    15241 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    15301 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    15361 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    15421 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    15481 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    15541 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    15601 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    15661 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    15721 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    15781 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    15841 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    15901 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    15961 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    16021 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    16081 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    16141 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    16201 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    16261 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    16321 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    16381 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    16441 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    16501 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    16561 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    16621 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    16681 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    16741 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    16801 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    16861 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    16921 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    16981 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    17041 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac
//
