LOCUS       473-d0-Mito-V5-BS2      5321 bp ds-DNA     circular     03-APR-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          complement(245..265)
                     /label="TL-0120"
                     /note="sequence: CCAGTGGCGATAAGTCGTGTC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(427..453)
                     /label="JY-0271"
                     /note="sequence: GCTACCAGCGGTGGTTTGTTTGCCGGA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(427..453)
                     /label="JY-0287"
                     /note="sequence: GCTACCAGCGGTGGTTTGTTTGCCGGA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          428..454
                     /label="BR-0017"
                     /note="sequence: CCGGCAAACAAACCACCGCTGGTAGCG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          577..598
                     /label="BR-0019"
                     /note="sequence: GTCATGAGATTATCAAAAAGGA"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    623..908
                     /label="Cer region"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(846..870)
                     /label="BR-0039"
                     /note="sequence: CATAATCCCTTAATTGTACGCACCG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          855..888
                     /label="KJ-1200"
                     /note="sequence: CAATTAAGGGATTATGGTAAATCCACTTACTGTC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          871..898
                     /label="JY-0059 (with modifications)"
                     /note="sequence: gtaaatccacttactgtctgccctcgta"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          886..907
                     /label="KJ-1202"
                     /note="sequence: GTCTGCCCTCGTAGCCATCGAG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(890..912)
                     /label="BR-0033"
                     /note="sequence: TTTATCTCGATGGCTACGAGGGC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          917..934
                     /label="JD-0627"
                     /note="sequence: agtactccggccacgatg"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(933..959)
                     /label="SR-1694"
                     /note="sequence: AGATCTCGATCCTCTACGCCGGACGCA"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          935..954
                     /label="BR-0627"
                     /note="sequence: cgtccggcgtagaggatcga"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          935..994
                     /label="BR-0272"
                     /note="sequence: cgtccggcgtagaggatcgagatcttcaatattggccattagccatattattcattggtt"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(937..959)
                     /label="JD-0180"
                     /note="sequence: agatctcgatcctctacgccgga"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(938..978)
                     /label="BR-0271"
                     /note="sequence: ggctaatggccaatattgaAGATCTCGATCCTCTACGCCGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     promoter        960..1701
                     /label="CMV immediate/early promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1019..1044
                     /label="DL-0242"
                     /note="sequence: ATTGGCCATTGCATACGTTGTATCTA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1173..1200
                     /label="KJ-0512"
                     /note="sequence: CGTTACATAACTTACGGTAAATGGCCCG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1597..1655
                     /label="KJ-1225"
                     /note="sequence: tttccaaaatgtcgtaataaccccgccccgttgacgcaaatgggcggtaggcgtgtacg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1618..1638
                     /label="BR-0021"
                     /note="sequence: CCCGCCCCGTTGACGCAAATG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1631..1650
                     /label="BR-0139"
                     /note="sequence: CGCAAATGGGCGGTAGGCGT"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          1631..1651
                     /label="CMVf"
                     /note="sequence: CGCAAATGGGCGGTAGGCGTG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1636..1655
                     /label="HZ-335"
                     /note="sequence: atgggcggtaggcgtgtacg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(1673..1711)
                     /label="DL-0428"
                     /note="sequence: agcttctagtgatctgacggttcactaaaccagctctgc"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1732..1753
                     /label="BR-0040"
                     /note="sequence: AGTTAAATTGCTAACGCAGTCA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1741..1760
                     /label="JC-22"
                     /note="sequence: GCTAACGCAGTCAGTGCTTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     intron          1816..1948
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1852..1875
                     /label="SR-0362"
                     /note="sequence: caatagaaactgggcttgtcgaga"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1949..1970
                     /label="JD-0178"
                     /note="sequence: gtgtccactcccagttcaatta"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1949..1976
                     /label="KJ-1369"
                     /note="sequence: gtgtccactcccagttcaattacagctc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1950..1970
                     /label="SR-0447"
                     /note="sequence: tgtccactcccagttcaatta"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1957..1978
                     /label="SR-0460"
                     /note="sequence: tcccagttcaattacagctctt"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1957..1985
                     /label="SR-1910"
                     /note="sequence: tcccagttcaattacagctcttaaggcta"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1957..1985)
                     /label="SR-1911"
                     /note="sequence: tagccttaagagctgtaattgaactggga"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(1958..1997)
                     /label="BR-0291"
                     /note="sequence: GGTGGCATACTCTAGCCTTAAGAGCTGTAATTGAACTGGG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1958..1997)
                     /label="YC-0004"
                     /note="sequence: GGTGGCATACTCTAGCCTTAAGAGCTGTAATTGAACTGGG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(1962..1997)
                     /label="BR-0597"
                     /note="sequence: GGTGGCatactctagccttaagagctgtaattgaac"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(1962..2000)
                     /label="TL-0296"
                     /note="sequence: CATGGTGGCATACTCTAGCCTTAAGAGCTGTAATTGAAC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(1962..2000)
                     /label="DL-0286"
                     /note="sequence: CATGGTGGCatactctagccttaagagctgtaattgaac"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1963..1991)
                     /label="JD-0082"
                     /note="sequence: atactctagccttaagagctgtaattgaa"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          1964..2013
                     /label="TQ-0096"
                     /note="sequence: tcaattacagctcttaaggctagagtatGCCACCATGCTGGCTACACGTG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1966..2024
                     /label="SP-41"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(1971..2050)
                     /label="SP-37"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(1971..2098)
                     /label="SP-33"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1974..2032)
                     /label="TQ-0094"
                     /note="sequence: TTCCCTACCAGAGAAAATACACGTGTAGCCAGCATGGTGGCatactctagccttaagag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1979..1997
                     /label="overlap"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1981..2000)
                     /label="JD-1066"
                     /note="sequence: catGGTGGCatactctagcc"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1998..2069
                     /label="MTS-Matrix"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1998..2069
                     /label="Translation 1998-2069"
                     /translation="MLATRVFSLVGKRAISTSVCVRAH"
     misc_feature    2031..2089
                     /label="SP-36"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(2046..2104)
                     /label="SP-42"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2070..2087
                     /label="SP-43"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2070..2111
                     /label="V5-tag"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2083..2141
                     /label="SP-34"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2112..2126
                     /label="KK420"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2112..2135
                     /label="KK424"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2112..2152
                     /label="KK425"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          2112..2155
                     /label="SP-0019"
                     /note="sequence: ACTCATCAAATAGTAACGACTCAATACGGCAAAGTAAAAGGCAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    2112..3581
                     /label="BS2"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             2112..3581
                     /label="Translation 2112-3581"
                     /translation="THQIVTTQYGKVKGTTENGVHKWKGIPYAKPPVGQWRFKAPEPPEVWEDVLDATAYGSICPQPSDLLSLSYTELPRQSEDCLYVNVFAPDTPSKNLPVMVWIHGGAFYLGAGSEPLYDGSKLAAQGEVIVVTLNYRLGPFGFLHLSSFNEAYSDNLGLLDQAAALKWVRENISAFGGDPDNVTVFGESAGGMSIAALLAMPAAKGLFQKAIMESGASRTMTKEQAASTSAAFLQVLGINEGQLDKLHTVSAEDLLKAADQLRIAEKENIFQLFFQPALDPKTLPEEPEKAIAEGAASGIPLLIGTTRDEGYLFFTPDSDVHSQETLDAALEYLLGKPLAEKVADLYPRSLESQIHMMTDLLFWRPAVAYASAQSHYAPVWMYRFDWHPKKPPYNKAFHALELPFVFGNLDGLERMAKAEITDEVKQLSHTIQSAWITFAKTGNPSTEAVNWPAYHEETRETLILDSEITIENDPESEKRQKLFPSKGEGS"
     primer          complement(2115..2141)
                     /label="HZ-388"
                     /note="sequence: GCCGTATTGAGTCGTTACTATTTGATG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(2115..2141)
                     /label="SP-0029"
                     /note="sequence: GCCGTATTGAGTCGTTACTATTTGATG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2118..2167
                     /label="HZ-345"
                     /note="sequence: CAAATAGTAACGACTCAATACGGCAAAGTAAAAGGCACAACGGAAAACGG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(2118..2167)
                     /label="HZ-346"
                     /note="sequence: CCGTTTTCCGTTGTGCCTTTTACTTTGCCGTATTGAGTCGTTACTATTTG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2367..2390
                     /label="KJ-0708"
                     /note="sequence: GTATTTGCGCCTGACACCCCAAGT"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(2367..2390)
                     /label="KJ-0484"
                     /note="sequence: ACTTGGGGTGTCAGGCGCAAATAC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          2391..2411
                     /label="KJ-0488"
                     /note="sequence: AAAAATCTTCCTGTCATGGTG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2391..2414)
                     /label="KJ-0649"
                     /note="sequence: CCACACCATGACAGGAAGATTTTT"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          2526..2549
                     /label="KJ-0710"
                     /note="sequence: CCGTTTGGCTTTTTGCACTTGTCT"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(2526..2549)
                     /label="KJ-0709"
                     /note="sequence: AGACAAGTGCAAAAAGCCAAACGG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          2600..2618
                     /label="KJ-0711"
                     /note="sequence: CGCGCTGAAATGGGTGCGA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2600..2618)
                     /label="KJ-0712"
                     /note="sequence: TCGCACCCATTTCAGCGCG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2757..2781
                     /label="KJ-0322"
                     /note="sequence: GCTTCTCGAACGATGACGAAAGAAC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2757..2781)
                     /label="KJ-0713"
                     /note="sequence: GTTCTTTCGTCATCGTTCGAGAAGC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          3108..3131
                     /label="KJ-0714"
                     /note="sequence: TTACTAGGGAAGCCGCTGGCAGAG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(3108..3131)
                     /label="KJ-0715"
                     /note="sequence: CTCTGCCAGCGGCTTCCCTAGTAA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          3517..3542
                     /label="KJ-0716"
                     /note="sequence: CGATCGAAAACGATCCCGAATCTGAA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(3517..3542)
                     /label="KJ-0717"
                     /note="sequence: TTCAGATTCGGGATCGTTTTCGATCG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(3536..3581)
                     /label="KK-0391"
                     /note="sequence: GGATCCTTCTCCTTTTGAAGGGAATAGCTTCTGCCTTTTTTCAGAT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(3554..3581)
                     /label="SP-32"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(3554..3611)
                     /label="SP-35"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          3560..3581
                     /label="KJ-0650"
                     /note="sequence: CCCTTCAAAAGGAGAAGGATCC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(3562..3581)
                     /label="KJ-0321"
                     /note="sequence: GGATCCTTCTCCTTTTGAAG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             3582..3584
                     /label="Translation 3582-3584"
                     /translation="*"
     misc_feature    3582..3612
                     /label="SP-31"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          3585..3606
                     /label="BR-0598"
                     /note="sequence: tgtttaaacgaattcgggctcg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          3585..3608
                     /label="TL-0373"
                     /note="sequence: TGTTTAAACGAATTCGGGCTCGGT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          3585..3611
                     /label="BR-0203"
                     /note="sequence: tgtttaaAcgaattcgggctcggtacc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          3604..3626
                     /label="SR-1915"
                     /note="sequence: tcggtacccggggatcctctaga"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(3604..3626)
                     /label="SR-1916"
                     /note="sequence: tctagaggatccccgggtaccga"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          3609..3632
                     /label="BR-0046"
                     /note="sequence: ACCCGGGGATCCTCTAGAGTCGAC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(3627..3647)
                     /label="BR-0245"
                     /note="sequence: CTTGCATGCCTGCAGGTCGAC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          3650..3669
                     /label="HZ-200"
                     /note="sequence: GATCCGGCTGCTAACAAAGC"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          3650..3671
                     /label="TL-0270"
                     /note="sequence: GATCCGGCTGCTAACAAAGCCC"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          3650..3671
                     /label="BR-0468"
                     /note="sequence: GATCCGGCTGCTAACAAAGCCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          3650..3678
                     /label="BR-0866"
                     /note="sequence: GATCCGGCTGCTAACAAAGCCCGAAAGGA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          3650..3678
                     /label="TL-0308"
                     /note="sequence: GATCCGGCTGCTAACAAAGCCCGAAAGGA"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          3666..3695
                     /label="BR-0051"
                     /note="sequence: AAGCCCGAAAGGAAGCTGAGTTGGCTGCTG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3666..3695)
                     /label="BR-0052"
                     /note="sequence: CAGCAGCCAACTCAGCTTCCTTTCGGGCTT"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          3679..3700
                     /label="BR-0037"
                     /note="sequence: AGCTGAGTTGGCTGCTGCCACC"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(3699..3717)
                     /label="BR-0811"
                     /note="sequence: GCTAGTTATTGCTCAGCGG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3699..3717)
                     /label="T7term"
                     /note="sequence: GCTAGTTATTGCTCAGCGG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          3724..3746
                     /label="KJ-0987"
                     /note="sequence: ccttggggcggccgcttcgagca"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          3731..3757
                     /label="KJ-1340"
                     /note="sequence: GCGGCCGCTTCGAGCAGACATGATAAG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(3741..3761)
                     /label="JC-23"
                     /note="sequence: GTATCTTATCATGTCTGCTCG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3745..3966
                     /label="SV40 late polyadenylation signal"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          3888..3919
                     /label="SR-0653"
                     /note="sequence: TGCATTCATTTTATGTTTCAGGTTCAGGGGGA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(3888..3919)
                     /label="SR-1517"
                     /note="sequence: TCCCCCTGAACCTGAAACATAAAATGAATGCA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          3939..3964
                     /label="DL-0767"
                     /note="sequence: agcaagtaaaacctctacaaatgtgg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(3939..3964)
                     /label="DL-0758"
                     /note="sequence: ccacatttgtagaggttttacttgct"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(3939..3977)
                     /label="KJ-0898"
                     /note="sequence: gaattcgattttaccacatttgtagaggttttacttgct"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(3944..3977)
                     /label="KJ-0769"
                     /note="sequence: gaattcgattttaccacatttgtagaggttttac"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(3961..3996)
                     /label="KJ-0989"
                     /note="sequence: gcgcaaagaggatccattagaattcgattttaccac"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(3970..3996)
                     /label="BR-0044"
                     /note="sequence: GCGCAAAGAGGATCCATTAGAATTCGA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(3970..3996)
                     /label="DL-0521"
                     /note="sequence: gcgcaaagaggatccattagaattcga"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          3978..4000
                     /label="KJ-0861"
                     /note="sequence: taatggatcctctttgcgcttgc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          3978..4002
                     /label="KJ-0899"
                     /note="sequence: taatggatcctctttgcgcttgcgt"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(4001..4024)
                     /label="KJ-0771"
                     /note="sequence: tgggctatctggacaagggaaaac"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          4061..4085
                     /label="BR-0312"
                     /note="sequence: cggactggctttctacgtgttccgc"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          4214..4273
                     /label="BR-0102"
                     /note="sequence: aggatctgatggcgcaggggatcaagatctgatcaagagacaggatgacggtcgtttcgc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(4219..4241)
                     /label="BR-0101"
                     /note="sequence: ATCTTGATCCCCTGCGCCATCAG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          4242..4267
                     /label="BR-0100"
                     /note="sequence: ctgatcaagagacaggatgacggtcg"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            4274..5068
                     /label="kanR gene"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             4274..5068
                     /label="kanR CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          4462..4484
                     /label="BR-0038"
                     /note="sequence: GGCAGCGCGGCTATCGTGGCTGG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(4511..4535)
                     /label="KJ-0753"
                     /note="sequence: CCGCTTCAGTGACAACGTCGAGCAC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          4580..4603
                     /label="KJ-0754"
                     /note="sequence: CTGTCATCTCACCTTGCTCCTGCC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          4604..4632
                     /label="SR-0498"
                     /note="sequence: gagaaagtatccatcatggctgatgcaat"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(4604..4632)
                     /label="SR-0499"
                     /note="sequence: attgcatcagccatgatggatactttctc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          4924..4948
                     /label="DL-0740"
                     /note="sequence: ctatcaggacatagcgttggctacc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          5021..5051
                     /label="DL-1352"
                     /note="sequence: gattcgcagcgcatcgccttctatcgccttc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          5069..5091
                     /label="KJ-1694"
                     /note="sequence: GCGGGACTCTGGGGTTCGAAATG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          5123..5154
                     /label="BR-0053"
                     /note="sequence: TCACTCAAAGGCGGTAATACGGTTATCCACAG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(5123..5154)
                     /label="BR-0054"
                     /note="sequence: CTGTGGATAACCGTATTACCGCCTTTGAGTGA"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(5202..5221)
                     /label="KJ-0353"
                     /note="sequence: TTTTTACGGTTCCTGGCCTT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      5237..5273
                     /label="ColE1-derived rep origin"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(5279..5298)
                     /label="TL-0107"
                     /note="sequence: ACCTCTGACTTGAGCGTCGA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
       61 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      121 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      181 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      241 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      301 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
      361 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
      421 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
      481 attacgcgca gaaaaaaagg atttcaagaa gatcctttga tcttttctac ggggtctgac
      541 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
      601 ttcacctaga tccttttata gtccggaaat acaggaacgc acgctggatg gcccttcgct
      661 gggatggtga aaccatgaaa aatggcagct tcagtggatt aagtgggggt aatgtggcct
      721 gtaccctctg gttgcatagg tattcatacg gttaaaattt atcaggcgcg attgcggcag
      781 tttttcgggt ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg ctgaacgcgt
      841 tttagcggtg cgtacaatta agggattatg gtaaatccac ttactgtctg ccctcgtagc
      901 catcgagata aaccgcagta ctccggccac gatgcgtccg gcgtagagga tcgagatctt
      961 caatattggc cattagccat attattcatt ggttatatag cataaatcaa tattggctat
     1021 tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca
     1081 atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc aattacgggg
     1141 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     1201 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1261 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1321 cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac
     1381 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg
     1441 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc
     1501 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     1561 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc
     1621 gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     1681 ggtttagtga accgtcagat cactagaagc tttattgcgg tagtttatca cagttaaatt
     1741 gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag aagttggtcg
     1801 tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa
     1861 ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact
     1921 gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcttaa
     1981 ggctagagta tGCCACCATG CTGGCTACAC GTGTATTTTC TCTGGTAGGG AAGCGTGCTA
     2041 TCTCTACATC TGTATGCGTA CGTGCTCACg gcaagcccat ccccaacccc ctgctgggcc
     2101 tggacagcac cACTCATCAA ATAGTAACGA CTCAATACGG CAAAGTAAAA GGCACAACGG
     2161 AAAACGGCGT ACATAAGTGG AAAGGCATCC CCTATGCCAA GCCGCCTGTC GGACAATGGC
     2221 GTTTTAAAGC ACCTGAGCCG CCTGAAGTGT GGGAAGATGT GCTTGATGCC ACAGCGTACG
     2281 GCTCTATTTG CCCGCAGCCG TCTGATTTGC TGTCACTTTC GTATACTGAG CTGCCCCGCC
     2341 AGTCCGAGGA TTGCTTGTAT GTCAATGTAT TTGCGCCTGA CACCCCAAGT AAAAATCTTC
     2401 CTGTCATGGT GTGGATTCAC GGAGGCGCTT TTTATCTAGG AGCGGGCAGT GAGCCATTGT
     2461 ATGACGGATC AAAACTTGCG GCACAGGGAG AAGTCATTGT CGTTACATTG AACTATCGGC
     2521 TGGGGCCGTT TGGCTTTTTG CACTTGTCTT CATTTAATGA GGCGTATTCT GATAACCTTG
     2581 GGCTTTTAGA CCAAGCCGCC GCGCTGAAAT GGGTGCGAGA GAATATTTCA GCGTTTGGCG
     2641 GTGATCCCGA TAACGTAACA GTATTTGGAG AATCCGCCGG CGGGATGAGC ATTGCCGCGC
     2701 TGCTTGCTAT GCCTGCGGCA AAAGGCCTGT TCCAGAAAGC AATCATGGAA AGCGGCGCTT
     2761 CTCGAACGAT GACGAAAGAA CAAGCGGCGA GCACCTCGGC AGCCTTTTTA CAGGTCCTTG
     2821 GGATTAACGA GGGCCAACTG GATAAATTGC ATACGGTTTC TGCGGAAGAT TTGCTAAAAG
     2881 CGGCTGATCA GCTTCGGATT GCAGAAAAAG AAAATATCTT TCAGCTGTTC TTCCAGCCCG
     2941 CCCTTGATCC GAAAACGCTG CCTGAAGAAC CAGAAAAAGC GATCGCAGAA GGGGCTGCTT
     3001 CCGGTATTCC GCTATTAATT GGAACAACCC GTGATGAAGG ATATTTATTT TTCACCCCGG
     3061 ATTCAGACGT TCATTCTCAG GAAACGCTTG ATGCAGCGCT CGAGTATTTA CTAGGGAAGC
     3121 CGCTGGCAGA GAAAGTTGCC GATTTGTATC CGCGTTCTCT GGAAAGCCAA ATTCACATGA
     3181 TGACTGATTT ATTATTTTGG CGCCCTGCCG TCGCCTATGC ATCCGCACAG TCTCATTACG
     3241 CCCCTGTCTG GATGTACAGG TTCGATTGGC ACCCGAAGAA GCCGCCGTAC AATAAAGCGT
     3301 TTCACGCATT AGAGCTTCCT TTTGTCTTTG GAAATCTGGA CGGATTGGAA CGAATGGCAA
     3361 AAGCGGAGAT TACGGATGAG GTGAAACAGC TTTCTCACAC GATACAATCA GCGTGGATCA
     3421 CGTTCGCCAA AACAGGAAAC CCAAGCACCG AAGCTGTGAA TTGGCCTGCG TATCATGAAG
     3481 AAACGAGAGA GACGCTGATT TTAGACTCAG AGATTACGAT CGAAAACGAT CCCGAATCTG
     3541 AAAAAAGGCA GAAGCTATTC CCTTCAAAAG GAGAAGGATC Ctaatgttta aacgaattcg
     3601 ggctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctg atccggctgc
     3661 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     3721 accccttggg gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa
     3781 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     3841 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     3901 tgtttcaggt tcagggggag atgtgggagg tttttttaag caagtaaaac ctctacaaat
     3961 gtggtaaaat cgaattctaa tggatcctct ttgcgcttgc gttttccctt gtccagatag
     4021 cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtgt
     4081 tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgag cttcaaaaga
     4141 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
     4201 tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag agacaggatg
     4261 acggtcgttt cgcatgcttg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     4321 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     4381 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     4441 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     4501 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     4561 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     4621 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     4681 agcgaaacat cgcatcgagc gagcacgcac tcggatggaa gccggtcttg tcgatcagga
     4741 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4801 gcgtatgccg gatggtgagg atctcgtcgt gactcatggc gatgcctgct tgccgaatat
     4861 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4921 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4981 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     5041 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     5101 gcgacgccca accggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     5161 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5221 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5281 gacgctcaag tcagaggtgg cgaaacccga caggactata a
//