LOCUS       Exported               14773 bp ds-DNA     circular SYN 25-APR-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14773)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Apr 25, 2024 from SnapGene 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14773
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    524..548
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     primer_bind     629..646
                     /label=nos terminator FW
                     /note="color: 緑; sequence: TGCATGCCGGTCGATCTA"
     primer_bind     640..668
                     /label=NosT_Asc_infu
                     /note="color: 緑; sequence: 
                     Agatcccccggcgcgccgatctagtaacatagatgacaccgcgcg; added: 
                     2023-12-06"
     primer_bind     640..653
                     /label=Cre_RV
                     /note="color: 緑; sequence: 
                     agcctcgaattcgtaacccccgatctagtaacat; added: 2023-12-06"
     terminator      641..893
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     primer_bind     641..667
                     /label=35SpIbMybNosT_SbfI
                     /note="color: 緑; sequence: 
                     cctgcagGATCTAGTAACATAGATGACACCGCGC"
     primer_bind     641..667
                     /label=6. BBMcassette_R
                     /note="color: 緑; sequence: 
                     TTTGTTTATTCATTATAAgatctagtaacatagatgacaccgcgc; added: 
                     2023-12-05"
     primer_bind     641..667
                     /label=8. BBMcassette_R
                     /note="color: 緑; sequence: 
                     TTTGTTTATTCATTATAAgatctagtaacatagatgacaccgcgc; added: 
                     2023-12-05"
     primer_bind     641..667
                     /label=infusionMYB-R
                     /note="color: 青; sequence: 
                     aaacactgatagtttgatctagtaacatagatgacaccgcgc; added: 
                     2023-12-06"
     primer_bind     complement(893..912)
                     /label=nos terminator RV
                     /note="color: 緑; sequence: 
                     CTCCCATATGGTCGAAATTCCCTAGAGTCAAGCAG; added: 2019-12-01"
     CDS             complement(1091..2116)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /note="color: #ccffcc"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     1141..1163
                     /label=HygforProbe_Rev
                     /note="color: 緑; sequence: CGAGTACTTCTACACAGCCATCG; added: 
                     2023-12-06"
     primer_bind     complement(1808..1827)
                     /label=HygforProbe_For
                     /note="color: 緑; sequence: GTGTCACGTTGCAAGACCTG; added: 
                     2023-12-06"
     primer_bind     1986..2009
                     /label=HPTr(exci.check)
                     /note="color: 緑; sequence: cgccctcctacatcgaagctgaaa; added:
                     2023-12-06"
     promoter        complement(2152..2331)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     2424..2457
                     /label=BBM WUS FW
                     /note="color: 緑; sequence: 
                     CGGTACCCGGGGATCAATCAAAGGCCATGGAGTC"
     primer_bind     2562..2583
                     /label=IbMYB_35S_Rev
                     /note="color: 青; sequence: 
                     gtctagatatctcgagccttggtggagcacgacacacttgt; added: 
                     2023-12-06"
     primer_bind     2566..2583
                     /label=infusion BBM FW
                     /note="color: 緑; sequence: 
                     GCACCAAGAGACTAGGGAGCACGACACACTTGT"
     primer_bind     2605..2629
                     /label=infusionMYB-F
                     /note="color: 青; sequence: 
                     tcccgccttcagtttacagtctcagaagaccaaagggca; added: 2023-12-06"
     primer_bind     2606..2629
                     /label=Pme_35SpIbMybNosT
                     /note="color: 緑; sequence: gtttaaaCAGTCTCAGAAGACCAAAGGGCA"
     primer_bind     2611..2628
                     /label=Asc1_CaMV35S FW
                     /note="color: 緑; sequence: GGCGCGCCCTCAGAAGACCAAAGGGC"
     primer_bind     2614..2645
                     /label=MinimumCaMV_inf_F
                     /note="color: 緑; sequence: 
                     TCGAGATGACCAAAGGGCAATTGAGACTTTTCAAC; added: 2020-02-26"
     promoter        2632..2977
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
                     /note="color: #ffcc99; direction: RIGHT"
     primer_bind     2983..2996
                     /label=CRE_NosT_Rev
                     /note="color: 青; sequence: tcctgcaggtcgactctagaggatc; 
                     added: 2023-12-06"
     primer_bind     complement(2985..3007)
                     /label=CaMV35S_Asc1 RV
                     /note="color: 緑; sequence: GGCGCGCCTGGTAATTGTGGATCCTCTAGAG"
     enhancer        3006..3030
                     /label=omega sequence
                     /note="color: #99cc00"
     CDS             3048..4799
                     /codon_start=1
                     /label=BBM
                     /note="color: #ff99cc"
                     /translation="MNSMNNWLGFSLSPHDQNHHRTDVDSSTTRTAVDVAGGYCFDLAA
                     PSDESSAVQTSFLSPFGVTLEAFTRDNNSHSRDWDINGGACNNINNNEQNGPKLENFLG
                     RTTTIYNTNETVVDGNGDCGGGDGGGGGSLGLSMIKTWLSNHSVANANHQDNGNGARGL
                     SLSMNSSTSDSNNYNNNDDVVQEKTIVDVVETTPKKTIESFGQRTSIYRGVTRHRWTGR
                     YEAHLWDNSCKREGQTRKGRQVYLGGYDKEEKAARAYDLAALKYWGTTTTTNFPLSEYE
                     KEVEEMKHMTRQEYVASLRRKSSGFSRGASIYRGVTRHHQHGRWQARIGRVAGNKDLYL
                     GTFGTQEEAAEAYDIAAIKFRGLSAVTNFDMNRYNVKAILESPSLPIGSSAKRLKDVNN
                     PVPAMMISNNVSESANNVSGWQNTAFQHHQGMDLSLLQQQQERYVGYYNGGNLSTESTR
                     VCFKQEEEQQHFLRNSPSHMTNVDHHSSTSDDSVTVCGNVVSYGGYQGFAIPVGTSVNY
                     DPFTAAEIAYNARNHYYYAQHQQQQQIQQSPGGDFPVAISNNHSSNMYFHGEGGGEGAP
                     TFSVWNDT"
     primer_bind     3048..3074
                     /label=AtBBM_CDS_For
                     /note="color: 緑; sequence: ATGAACTCGATGAATAACTGGTTAGGC; 
                     added: 2019-09-10"
     primer_bind     3048..3074
                     /label=AvrII-BBM3xHAnosT_For
                     /note="color: 緑; sequence: 
                     cctaggATGAACTCGATGAATAACTGGTTAGGC; added: 2023-12-05"
     primer_bind     3117..3136
                     /label=qRT_BBMfor
                     /note="color: 紫; sequence: gttgactcctccaccaccag; added: 
                     2023-10-31"
     primer_bind     complement(3147..3168)
                     /label=BBM_seq1R
                     /note="color: 緑; sequence: aacagtaccctccggcaacatc; added: 
                     2023-12-05"
     primer_bind     complement(3226..3245)
                     /label=qRT_BBM1rev
                     /note="color: 紫; sequence: ttcgagggtgacaccgaaag; added: 
                     2023-10-31"
     primer_bind     3852..3875
                     /label=BBM_seq1F
                     /note="color: 緑; sequence: accaccactactactaacttcccc; added:
                     2023-12-05"
     primer_bind     4346..4367
                     /label=BBM_seq2F
                     /note="color: 緑; sequence: gagcttattgcagcaacagcag; added: 
                     2023-12-05"
     primer_bind     4428..4452
                     /label=bbm8-1
                     /note="color: 緑; sequence: ttcaaacaagaggaggaacaacaac; 
                     added: 2023-11-21"
     primer_bind     complement(4510..4534)
                     /label=bbm8-2
                     /note="color: 緑; sequence: ccacaaacggtaacagaatcatcag; 
                     added: 2023-11-21"
     primer_bind     4520..4543
                     /label=bbm10-1
                     /note="color: 緑; sequence: tgttaccgtttgtggaaatgttgt; added:
                     2023-12-05"
     primer_bind     4524..4547
                     /label=bbm5-1
                     /note="color: 緑; sequence: accgtttgtggaaatgttgttagt; added:
                     2023-12-05"
     primer_bind     complement(4604..4626)
                     /label=bbm5-2
                     /note="color: 緑; sequence: aagcaatctcagcagcagtaaag; added: 
                     2023-12-05"
     primer_bind     complement(4661..4682)
                     /label=bbm10-2
                     /note="color: 緑; sequence: ctgaatctgctgttgttgctga; added: 
                     2023-12-05"
     primer_bind     complement(4775..4799)
                     /label=AtBBM_CDS_stop_Rev
                     /note="color: 緑; sequence: AGTGTCGTTCCAAACTGAAAACGTT; 
                     added: 2023-12-05"
     primer_bind     complement(4777..4799)
                     /label=AtBBM_CDS_Rev
                     /note="color: 緑; sequence: ctaagtgtcgttccaaactgaaaacg; 
                     added: 2019-09-10"
     primer_bind     complement(4777..4799)
                     /label=infusion35SBBMrev
                     /note="color: 緑; sequence: 
                     ATATgagctcGTCGACCTAAGTGTCGTTCCAAACTGAAAACG; added: 
                     2023-12-05"
     CDS             4803..5036
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /note="color: #ff0000"
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     terminator      5052..5301
                     /label=HSPter
                     /note="color: #999999"
     primer_bind     5299..5334
                     /label=infusion RPS5A F
                     /note="color: 緑; sequence: 
                     AAGGAATTCGCGGCCTTTCAATTTCATGGTGAGGAT"
     primer_bind     5314..5335
                     /label=RPS5A_For
                     /note="color: 青; sequence: 
                     gaccaaagggcaattttcaatttcatggtgaggata; added: 2023-12-06"
     promoter        5315..5998
                     /label=RPS5A promoter
                     /note="color: #ff6600; direction: RIGHT"
     primer_bind     5683..5711
                     /label=Hpa1_RPS5A_inf_F
                     /note="color: 緑; sequence: aatattccgtaagttaaccggctaaaacc; 
                     added: 2020-11-19"
     primer_bind     5976..5998
                     /label=IbMYB_NosT_For
                     /note="color: 青; sequence: 
                     agtcgacctgcaggaaggctctgtttctctcaccacagcc; added: 
                     2023-12-06"
     primer_bind     5976..5998
                     /label=Eco53del_loxP_For
                     /note="color: 青; sequence: 
                     gattacgaattcgagGCTCTGTTTCTCTCACCACAGCC; added: 2023-12-06"
     primer_bind     complement(5977..6006)
                     /label=RPS5A_inf_Nru1_R
                     /note="color: 緑; sequence: GGATCCTCGGCTGTGGTGAGAGAAACagag; 
                     added: 2020-11-19"
     primer_bind     complement(5981..5998)
                     /label=infusion RPS5A R
                     /note="color: 緑; sequence: 
                     CTGTGGCGGCTCCATTGGCTGTGGTGAGAGAAAC"
     primer_bind     complement(5981..5998)
                     /label=RPS5A_Rev
                     /note="color: 青; sequence: 
                     tcgcgaattcaagctggctgtggtgagagaaac; added: 2023-12-06"
     CDS             6058..6975
                     /codon_start=1
                     /label=SDRX_WUS
                     /note="color: #a6acb3"
                     /translation="MDLDLELRLGFAPMEPPQHQHHHHQADQESGNNNNNKSGSGGYTC
                     RQTSTRWTPTTEQIKILKELYYNNAIRSPTADQIQKITARLRQFGKIEGKNVFYWFQNH
                     KARERQKKRFNGTNMTTPSSSPNSVMMAANDHYHPLLHHHHGVPMQRPANSVNVKLNQD
                     HHLYHHNKPYPSFNNGNLNHASSGTECGVVNASNGYMSSHVYGSMEQDCSMNYNNVGGG
                     WANMDHHYSSAPYNFFDRAKPLFGLEGHQEEEECGGDAYLEHRRTAPAFPMHGEDHING
                     GSGAIWKYGQSEVRPCASLELRLN"
     CDS             6063..6975
                     /codon_start=1
                     /label=改変WUS
                     /note="color: #3366ff"
                     /translation="LDLELRLGFAPMEPPQHQHHHHQADQESGNNNNNKSGSGGYTCRQ
                     TSTRWTPTTEQIKILKELYYNNAIRSPTADQIQKITARLRQFGKIEGKNVFYWFQNHKA
                     RERQKKRFNGTNMTTPSSSPNSVMMAANDHYHPLLHHHHGVPMQRPANSVNVKLNQDHH
                     LYHHNKPYPSFNNGNLNHASSGTECGVVNASNGYMSSHVYGSMEQDCSMNYNNVGGGWA
                     NMDHHYSSAPYNFFDRAKPLFGLEGHQEEEECGGDAYLEHRRTAPAFPMHGEDHINGGS
                     GAIWKYGQSEVRPCASLELRLN"
     misc_feature    6064..6093
                     /label=SRDX
                     /note=" LDLDLELRLGFA"
                     /note="color: #ffff00"
     primer_bind     6096..6116
                     /label=avr2_WUS
                     /note="color: 緑; sequence: 
                     agtcgtcctaggATGGAGCCGCCACAGCATCA; added: 2023-12-05"
     CDS             6097..6975
                     /codon_start=1
                     /label=WUS-mutation (WUSm1)
                     /note="color: #99ccff"
                     /translation="MEPPQHQHHHHQADQESGNNNNNKSGSGGYTCRQTSTRWTPTTEQ
                     IKILKELYYNNAIRSPTADQIQKITARLRQFGKIEGKNVFYWFQNHKARERQKKRFNGT
                     NMTTPSSSPNSVMMAANDHYHPLLHHHHGVPMQRPANSVNVKLNQDHHLYHHNKPYPSF
                     NNGNLNHASSGTECGVVNASNGYMSSHVYGSMEQDCSMNYNNVGGGWANMDHHYSSAPY
                     NFFDRAKPLFGLEGHQEEEECGGDAYLEHRRTAPAFPMHGEDHINGGSGAIWKYGQSEV
                     RPCASLELRLN"
     primer_bind     6097..6116
                     /label=asc1_WUS
                     /note="color: 緑; sequence: 
                     acgatcGGCGCGCCATGGAGCCGCCACAGCATCA; added: 2023-12-05"
     primer_bind     6097..6114
                     /label=infusion WUS F
                     /note="color: 緑; sequence: ATGGAGCCGCCACAGCAT; added: 
                     2023-12-05"
     primer_bind     6323..6344
                     /label=wus1-1
                     /note="color: 緑; sequence: tcggaaagattgagggcaagaa; added: 
                     2023-12-05"
     primer_bind     6324..6344
                     /label=wus6-1
                     /note="color: 緑; sequence: CGGAAAGATTGAGGGCAAGAA; added: 
                     2023-11-27"
     primer_bind     complement(6418..6440)
                     /label=wus6-2
                     /note="color: 緑; sequence: GAGTTGGGTGATGAAGATGGTGT; added: 
                     2023-11-27"
     primer_bind     complement(6468..6492)
                     /label=wus1-2
                     /note="color: 緑; sequence: gtgatgatggtgaagtagaggatga; 
                     added: 2023-12-05"
     primer_bind     6649..6670
                     /label=WUSseq_F1
                     /note="color: 緑; sequence: ggctacatgagtagccatgtct; added: 
                     2020-11-19"
     primer_bind     6718..6737
                     /label=qRT_WUS1_For
                     /note="color: 紫; sequence: ggaggatgggcaaacatgga; added: 
                     2023-10-31"
     primer_bind     complement(6720..6739)
                     /label=WUSseq_R1
                     /note="color: 緑; sequence: Gatccatgtttgcccatcct; added: 
                     2020-11-19"
     misc_feature    6796..6837
                     /label=Acidic region
                     /note="color: #00ccff"
     primer_bind     complement(6846..6858)
                     /label=qRT_WUS1_Rev
                     /note="color: 紫; sequence: gaggaagcgtacgtcgatgt; added: 
                     2023-10-31"
     misc_feature    6856..6879
                     /label=WUS-box_mutation
                     /note="color: #ff0000"
     misc_feature    6859..6861
                     /label=L-->A
                     /note="color: #ff0000"
     misc_feature    6865..6867
                     /label=L-->A
                     /note="color: #ff0000"
     primer_bind     complement(6947..6976)
                     /label=infusion WUS R
                     /note="color: 緑; sequence: 
                     ATCTCGAGTGCGGCCCTAGTTCAGACGTAGCTCAagagaagcgc; added: 
                     2023-12-05"
     primer_bind     complement(6947..6975)
                     /label=WUS_pac1 stop
                     /note="color: 緑; sequence: 
                     atggtcttaattaactagttcagacgtagctcaagagaagcgc; added: 
                     2023-12-05"
     primer_bind     complement(6947..6972)
                     /label=WUS_sal1 non-stop
                     /note="color: 緑; sequence: 
                     accatggtcgacGTTCAGACGTAGCTCAAGAGAAGCGC; added: 2023-12-05"
     primer_bind     complement(6948..6973)
                     /label=WUS_pac1 non_stop
                     /note="color: 緑; sequence: 
                     acgtcgttaattaaGTTCAGACGTAGCTCAAGAGAAGCG; added: 2023-12-05"
     misc_feature    6952..6972
                     /label=EAR-like motif
                     /note="color: #00ccff"
     primer_bind     complement(6957..6975)
                     /label=BBM WUS RV
                     /note="color: 緑; sequence: 
                     ATCGACCGGCATGCACTAGTTCAGACGTAGCTCA; added: 2023-12-05"
     terminator      6998..7250
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     primer_bind     complement(7223..7252)
                     /label=NosT_Asc_infu
                     /note="color: 緑; sequence: 
                     Agatcccccggcgcgccgatctagtaacatagatgacaccgcgcg; added: 
                     2023-12-06"
     primer_bind     complement(7224..7250)
                     /label=35SpIbMybNosT_SbfI
                     /note="color: 緑; sequence: 
                     cctgcagGATCTAGTAACATAGATGACACCGCGC"
     primer_bind     complement(7224..7250)
                     /label=6. BBMcassette_R
                     /note="color: 緑; sequence: 
                     TTTGTTTATTCATTATAAgatctagtaacatagatgacaccgcgc; added: 
                     2023-12-05"
     primer_bind     complement(7224..7250)
                     /label=8. BBMcassette_R
                     /note="color: 緑; sequence: 
                     TTTGTTTATTCATTATAAgatctagtaacatagatgacaccgcgc; added: 
                     2023-12-05"
     primer_bind     complement(7224..7250)
                     /label=infusionMYB-R
                     /note="color: 青; sequence: 
                     aaacactgatagtttgatctagtaacatagatgacaccgcgc; added: 
                     2023-12-06"
     primer_bind     complement(7238..7253)
                     /label=Cre_RV
                     /note="color: 緑; sequence: 
                     agcctcgaattcgtaacccccgatctagtaacat; added: 2023-12-06"
     primer_bind     complement(7256..7283)
                     /label=WUS_inf_R_20mer
                     /note="color: 緑; sequence: CTTTGGTCATCTCGAGTGCGGCCGcgaa; 
                     added: 2020-11-19"
     primer_bind     complement(7260..7275)
                     /label=infusion BBM RV
                     /note="color: 緑; sequence: 
                     gcactctcagtacaaatatctcgagtgcggccg; added: 2023-12-05"
     primer_bind     7269..7303
                     /label=MinimumCaMV_inf_F
                     /note="color: 緑; sequence: 
                     TCGAGATGACCAAAGGGCAATTGAGACTTTTCAAC; added: 2020-02-26"
     primer_bind     7272..7286
                     /label=Asc1_CaMV35S FW
                     /note="color: 緑; sequence: GGCGCGCCCTCAGAAGACCAAAGGGC"
     primer_bind     7276..7287
                     /label=Pme_35SpIbMybNosT
                     /note="color: 緑; sequence: gtttaaaCAGTCTCAGAAGACCAAAGGGCA"
     primer_bind     7276..7287
                     /label=infusionMYB-F
                     /note="color: 青; sequence: 
                     tcccgccttcagtttacagtctcagaagaccaaagggca; added: 2023-12-06"
     promoter        7290..7635
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
                     /note="color: #ffcc99; direction: RIGHT"
     primer_bind     complement(7650..7690)
                     /label=MinimumCaMV_inf_R
                     /note="color: 緑; sequence: 
                     CATTATGGATCCTCGATCCTCGCGAATTCAAGCTTCCATGG; added: 
                     2020-11-19"
     misc_feature    7688..8434
                     /label=IbMYB
                     /note="color: #800080; direction: RIGHT"
     primer_bind     7688..7711
                     /label=IbMyb_For
                     /note="color: 緑; sequence: atggttatttcatctgtatggtcg"
     primer_bind     7734..7759
                     /label=MYB_check_F
                     /note="color: 緑; sequence: AAGGTTCATGGTCCGAAGAAGAAGAC; 
                     added: 2023-12-05"
     primer_bind     7748..7776
                     /label=IbMyb_F_gPCR
                     /note="color: 緑; sequence: GAAGAAGAAGACCAACTTTTGAGGGAGTG; 
                     added: 2023-12-05"
     primer_bind     complement(7798..7817)
                     /label=IbMyb_seqR1
                     /note="color: 緑; sequence: GGGGAATTAGATGCCATTTT; added: 
                     2023-12-05"
     primer_bind     8118..8136
                     /label=IbMyb_seqF1
                     /note="color: 緑; sequence: GGCCTCGACCTCGGAGATT; added: 
                     2023-12-05"
     primer_bind     complement(8234..8258)
                     /label=IbMyb_R_gPCR
                     /note="color: 緑; sequence: CCATTAGCAAGTCCGACGTTGTTTC; 
                     added: 2023-12-05"
     primer_bind     complement(8366..8392)
                     /label=MYB_check_R
                     /note="color: 緑; sequence: ACTCCAACAGATTTGTCCTTCCTCATT; 
                     added: 2023-12-05"
     primer_bind     complement(8409..8432)
                     /label=IbMyb_Rev
                     /note="color: 緑; sequence: ttaacagttctgacagtaggtcta"
     terminator      8468..8720
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #c0c0c0"
     primer_bind     complement(8693..8720)
                     /label=NosT_Asc_infu
                     /note="color: 緑; sequence: 
                     Agatcccccggcgcgccgatctagtaacatagatgacaccgcgcg; added: 
                     2023-12-06"
     primer_bind     complement(8694..8735)
                     /label=infusionMYB-R
                     /note="color: 青; sequence: 
                     aaacactgatagtttgatctagtaacatagatgacaccgcgc; added: 
                     2023-12-06"
     primer_bind     complement(8694..8720)
                     /label=35SpIbMybNosT_SbfI
                     /note="color: 緑; sequence: 
                     cctgcagGATCTAGTAACATAGATGACACCGCGC"
     primer_bind     complement(8694..8720)
                     /label=6. BBMcassette_R
                     /note="color: 緑; sequence: 
                     TTTGTTTATTCATTATAAgatctagtaacatagatgacaccgcgc; added: 
                     2023-12-05"
     primer_bind     complement(8694..8720)
                     /label=8. BBMcassette_R
                     /note="color: 緑; sequence: 
                     TTTGTTTATTCATTATAAgatctagtaacatagatgacaccgcgc; added: 
                     2023-12-05"
     misc_feature    8735..8759
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     CDS             10090..10719
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /note="color: #993366"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11148..12221
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /note="color: #993366"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12287..12481
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /note="color: #ffff00"
     misc_feature    12825..12965
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     primer_bind     12895..12913
                     /label=infusion_Myb_loxP_FW
                     /note="color: 緑; sequence: TTGTACTGAGAGTGCACCA; added: 
                     2023-12-05"
     rep_origin      complement(13151..13739)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(join(13985..14773,1..3))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /note="color: #ccffcc"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 catgatgttt aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca
       61 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
      121 ctgtacccca aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc
      181 gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag
      241 cttacgaacc gaacaggctt atgtccactg ggttcgtgcc cgaattgatc acaggcagca
      301 acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac
      361 ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc
      421 ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg
      481 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg
      541 gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact
      601 gaattaacgc cgaattgaat tatcagcttg catgccggtc gatctagtaa catagatgac
      661 accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa
      721 atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt catgcattac
      781 atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga
      841 ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcttga
      901 ctctagggaa ttaattcctg aatcactgcg accggccctc ccgcgaccca gccgagcgag
      961 cttagcgaac tgtggacgag aactgtgcca ccaagcgtaa ggccgttctc tcgcatttgc
     1021 cttgctaggc tcgcgcgagt tgctggctga ggcgttctcg aaatcagctc ttgttcggtc
     1081 ggcatctact ctattccttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg
     1141 cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac
     1201 gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg
     1261 catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca
     1321 tatacgcccg gagccttggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg
     1381 tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt
     1441 gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg
     1501 tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct
     1561 cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca
     1621 tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg
     1681 tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat
     1741 cggccgcagc gatagcatcc atagcctccg cgaccggctg aagaacagcg ggcagttcgg
     1801 tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc
     1861 tctcgctgaa ctccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt
     1921 gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat
     1981 atccacgccc tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca
     2041 tcaggtcgga gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga
     2101 gttcaggctt tttcataggg gggattcgag ttgagagtga atatgagact ctaattggat
     2161 accgagggga atttatggaa cgtcagtgga gcatttttga caagaaatat ttgctagctg
     2221 atagtgacct taggcgactt ttgaacgcgc aataatggtt tctgacgtat gtgcttagct
     2281 cattaaactc cagaaacccg cggctcagtg gctccttcaa cgttgcggtt ctgtcagttc
     2341 caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt
     2401 ctccgctcat gatcgataat tcgcggtacc cggggatcaa tcaaaggcca tggagtcaaa
     2461 gattcaaata gaggacctaa cagaactcgc cgtaaagact ggcgaacagt tcatacagag
     2521 tctcttacga ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacacact
     2581 tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt
     2641 tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt
     2701 tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
     2761 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
     2821 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
     2881 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
     2941 ctctatataa ggaagttcat ttcatttgga gaggacacgg gggactctag aggatccaca
     3001 attaccaaca acaacaaaca acaaacaaca ttacaattac agatcccatg aactcgatga
     3061 ataactggtt aggcttctct ctctctcctc atgatcaaaa tcatcaccgt acggatgttg
     3121 actcctccac caccagaacc gccgtagatg ttgccggagg gtactgtttt gatctggccg
     3181 ctccctccga tgaatcttct gccgttcaaa catcttttct ttctcctttc ggtgtcaccc
     3241 tcgaagcttt caccagagac aataatagtc actcccgaga ttgggacatc aatggtggtg
     3301 catgcaataa cattaacaat aacgaacaaa atggaccaaa gcttgagaat ttcctcggcc
     3361 gcaccaccac gatttacaat accaacgaga ccgttgtaga tggaaatggc gattgtggag
     3421 gaggagacgg tggtggtggc ggctcactag gcctttcgat gataaaaaca tggctgagta
     3481 atcattcggt tgctaatgct aatcatcaag acaatggtaa cggtgcacga ggcttgtccc
     3541 tctctatgaa ttcatctact agtgatagca acaactacaa caacaatgat gatgtcgtcc
     3601 aagagaagac tattgttgat gtcgtagaaa ctacaccgaa gaaaactatt gagagttttg
     3661 gacaaaggac gtctatatac cgcggtgtta caaggcatcg gtggacaggt agatacgagg
     3721 cacatttatg ggacaatagt tgcaaaagag aaggccagac tcgcaaagga agacaagttt
     3781 atctgggagg ttatgacaaa gaagaaaaag cagctagggc ttacgattta gccgcactaa
     3841 agtattgggg aaccaccact actactaact tccccttgag tgaatatgag aaagaggtag
     3901 aagagatgaa gcacatgacg aggcaagagt atgttgcctc tctgcgcagg aaaagtagtg
     3961 gtttctctcg tggtgcatcg atttatcgag gagtaacaag gcatcaccaa catggaaggt
     4021 ggcaagctag gatcggaaga gtcgccggta acaaagacct ctacttggga actttcggca
     4081 cacaggaaga ggctgctgag gcttatgaca ttgcagccat taaattcaga ggattaagcg
     4141 cagtgactaa cttcgacatg aacagataca atgttaaagc aatcctcgag agcccgagtc
     4201 tacctattgg tagttctgcg aaacgtctca aggacgttaa taatccggtt ccagctatga
     4261 tgattagtaa taacgtttca gagagtgcaa ataatgttag cggttggcaa aacactgcgt
     4321 ttcagcatca tcagggaatg gatttgagct tattgcagca acagcaggag aggtacgttg
     4381 gttattacaa tggaggaaac ttgtctaccg agagtactag ggtttgtttc aaacaagagg
     4441 aggaacaaca acacttcttg agaaactcgc cgagtcacat gactaatgtt gatcatcata
     4501 gctcgacctc tgatgattct gttaccgttt gtggaaatgt tgttagttat ggtggttatc
     4561 aaggattcgc aatccctgtt ggaacatcgg ttaattacga tccctttact gctgctgaga
     4621 ttgcttacaa cgcaagaaat cattattact atgctcagca tcagcaacaa cagcagattc
     4681 agcagtcgcc gggaggagat tttccggtgg cgatttcgaa taaccatagc tctaacatgt
     4741 actttcacgg ggaaggtggt ggagaagggg ctccaacgtt ttcagtttgg aacgacactg
     4801 gggccccccc gaccgatgtc agcctggggg acgagctcca cttagacggc gaggacgtgg
     4861 cgatggcgca tgccgacgcg ctagacgatt tcgatctgga catgttgggg gacggggatt
     4921 ccccagggcc gggatttacc ccccacgact ccgcccccta cggcgctctg gatatggccg
     4981 acttcgagtt tgagcagatg tttaccgatg cccttggaat tgacgagtac ggtgggtagg
     5041 tcgacgagct catatgaaga tgaagatgaa atatttggtg tgtcaaataa aaagcttgtg
     5101 tgcttaagtt tgtgtttttt tcttggcttg ttgtgttatg aatttgtggc tttttctaat
     5161 attaaatgaa tgtaagatct cattataatg aataaacaaa tgtttctata atccattgtg
     5221 aatgttttgt tggatctctt ctgcagcata taactactgt atgtgctatg gtatggacta
     5281 tggaatatga ttaaagataa ggaattcgcg gcctttcaat ttcatggtga ggatatgcag
     5341 ttttctttgt atatcattct tcttcttctt tgtagcttgg agtcaaaatc ggttccttca
     5401 tgtacataca tcaaggatat gtccttctga atttttatat cttgcaataa aaatgcttgt
     5461 accaattgaa acaccagctt tttgagttct atgatcactg acttggttct aaccaaaaaa
     5521 aaaaaaatgt ttaatttaca tatctaaaag taggtttagg gaaacctaaa cagtaaaata
     5581 tttgtatatt attcgaattt cactcatcat aaaaacttaa attgcaccat aaaattttgt
     5641 tttactatta atgatgtaat ttgtgtaact taagataaaa ataatattcc gtaagttaac
     5701 cggctaaaac cacgtataaa ccagggaacc tgttaaaccg gttctttact ggataaagaa
     5761 atgaaagccc atgtagacag ctccattaga gcccaaaccc taaatttctc atctatataa
     5821 aaggagtgac attagggttt ttgttcgtcc tcttaaagct tctcgttttc tctgccgtct
     5881 ctctcattcg cgcgacgcaa acgatcttca ggtgatcttc tttctccaaa tcctctctca
     5941 taactctgat ttcgtacttg tgtatttgag ctcacgctct gtttctctca ccacagccga
     6001 ggatccacaa ttaccaacaa caacaaacaa caaacaacat tacaattaca gatcccgatg
     6061 gatctggatc tagaactccg tttgggtttc gccccgatgg agccgccaca gcatcagcat
     6121 catcatcatc aagccgacca agaaagcggc aacaacaaca acaacaagtc cggctctggt
     6181 ggttacacgt gtcgccagac cagcacgagg tggacaccga cgacggagca aatcaaaatc
     6241 ctcaaagaac tttactacaa caatgcaatc cggtcaccaa cagccgatca gatccagaag
     6301 atcactgcaa ggctgagaca gttcggaaag attgagggca agaacgtctt ttactggttc
     6361 cagaaccata aggctcgtga gcgtcagaag aagagattca acggaacaaa catgaccaca
     6421 ccatcttcat cacccaactc ggttatgatg gcggctaacg atcattatca tcctctactt
     6481 caccatcatc acggtgttcc catgcagaga cctgctaatt ccgtcaacgt taaacttaac
     6541 caagaccatc atctctatca tcataacaag ccatatccca gcttcaataa cgggaattta
     6601 aatcatgcaa gctcaggtac tgaatgtggt gttgttaatg cttctaatgg ctacatgagt
     6661 agccatgtct atggatctat ggaacaagac tgttctatga attacaacaa cgtaggtgga
     6721 ggatgggcaa acatggatca tcattactca tctgcacctt acaacttctt cgatagagca
     6781 aagcctctgt ttggtctaga aggtcatcaa gaagaagaag aatgtggtgg cgatgcttat
     6841 ctggaacatc gacgtacggc ccctgctttc cctatgcacg gtgaagatca catcaacggt
     6901 ggtagtggtg ccatctggaa gtatggccaa tcggaagttc gcccttgcgc ttctcttgag
     6961 ctacgtctga actaggtcga cgagctcgaa tttccccgat cgttcaaaca tttggcaata
     7021 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     7081 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     7141 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     7201 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattcgc
     7261 ggccgcactc gagatgacca aagggcaatt gagacttttc aacaaagggt aatatccgga
     7321 aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag
     7381 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc
     7441 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
     7501 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
     7561 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
     7621 catttggaga gaacacgggg gactcttgac catggaagct tgaattcgcg aggatcgagg
     7681 atccataatg gttatttcat ctgtatggtc gggatcgtct tcgagagtga gaaaaggttc
     7741 atggtccgaa gaagaagacc aacttttgag ggagtgcatt cagaaatatg gtgaaggaaa
     7801 atggcatcta attcccctta gagctggatt gaataggtgc agaaaaagtt gtagattaag
     7861 atggttgaat tatctccgtc ccgatataaa gagaggcgaa tttagtcccg atgaaattga
     7921 tctcattctg cgcctccata ggctcttagg caacaggtgg tcgcttattg ctggaagaat
     7981 tccgggaaga acagcaaacg atgtgaagaa tttatggaac acccatcttc agaagaaggt
     8041 gtctgccatg gcttcttcaa ggcaagataa ttattggaag ggcaaagccc cagaaatcac
     8101 ggaaaacacc gtcgttaggc ctcgacctcg gagattctta aaggcctcat catctccgac
     8161 gactctattg accggaaatg ctaccatggt tgcctatgat ggtcaactcc aagaacatat
     8221 gacgacacaa ccggaaacaa cgtcggactt gctaatggaa aatgtccaac aaaaaaactt
     8281 aacaaccact ttgccttcag cactagaaac aacgccacac gacaatgtga agtggtggga
     8341 agatgtactc tccgacaagg aactcaatga ggaaggacaa atctgttgga gtgagtttcc
     8401 aactgatata gacctactgt cagaactgtt aagctctgca gatctggtga ccagctcgaa
     8461 tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     8521 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     8581 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     8641 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     8701 gtcatctatg ttactagatc aaactatcag tgtttgacag gatatattgg cgggtaaacc
     8761 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     8821 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt
     8881 taaagtactt taaagtactt taaagtactt tgatccaacc cctccgctgc tatagtgcag
     8941 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag
     9001 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc
     9061 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg
     9121 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac
     9181 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca
     9241 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga
     9301 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc
     9361 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca
     9421 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc
     9481 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg
     9541 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg
     9601 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc
     9661 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc
     9721 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg
     9781 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat
     9841 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt
     9901 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag
     9961 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
    10021 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
    10081 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
    10141 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
    10201 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
    10261 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
    10321 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
    10381 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
    10441 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
    10501 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
    10561 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
    10621 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
    10681 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
    10741 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
    10801 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
    10861 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
    10921 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
    10981 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
    11041 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc
    11101 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca
    11161 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
    11221 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
    11281 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
    11341 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
    11401 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
    11461 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
    11521 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
    11581 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
    11641 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
    11701 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
    11761 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
    11821 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
    11881 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
    11941 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
    12001 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
    12061 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
    12121 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
    12181 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
    12241 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt
    12301 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
    12361 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
    12421 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
    12481 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
    12541 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
    12601 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
    12661 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
    12721 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
    12781 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
    12841 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
    12901 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    12961 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    13021 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    13081 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    13141 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    13201 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    13261 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    13321 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    13381 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    13441 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    13501 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    13561 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
    13621 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    13681 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    13741 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    13801 ggattttggt catgcatgat atatctccca atttgtgtag ggcttattat gcacgcttaa
    13861 aaataataaa agcagacttg acctgatagt ttggctgtga gcaattatgt gcttagtgca
    13921 tctaacgctt gagttaagcc gcgccgcgaa gcggcgtcgg cttgaacgaa tttctagcta
    13981 gacattattt gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca
    14041 actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt
    14101 caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat
    14161 ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta
    14221 catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta
    14281 gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta
    14341 ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg
    14401 tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt
    14461 cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta
    14521 cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca
    14581 aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac
    14641 tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg
    14701 gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga
    14761 tcaccgcttc ccc
//
