LOCUS       MSCV-LMP1-imC                 7585 bp    DNA     circular SYN 28-MAR-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(1703..2410)
                     /note="pLannotate"
                     /label="mCherry"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     LTR             complement(5064..5580)
                     /note="pLannotate"
                     /label="5' LTR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     LTR             complement(1087..1601)
                     /note="pLannotate"
                     /label="3' LTR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     misc_feature    complement(2424..2886)
                     /note="pLannotate"
                     /label="IRES"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             complement(4176..4592)
                     /note="pLannotate"
                     /label="gag (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    complement(4659..5000)
                     /note="pLannotate"
                     /label="MESV Ψ"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             complement(2999..4156)
                     /note="pLannotate"
                     /label="LMP1"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        6408..6512
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             6513..7373
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      join(7544..7585,1..547)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        871..901
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    835..856
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    909..925
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             5922..6071
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="86.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(6077..6176)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="70.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             5721..5922
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="17.0"
                     /fragment="True"
                     /other="CDS"
     CDS             730..822
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(6278..6313)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(1660..1686)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="25.0"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(5622..5720)
                     /note="pLannotate"
                     /label="env (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.0"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      721 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      781 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      841 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      901 gtgtggaatt gtgagcggat aacaatttca caattctccc cagctctttc tgcgctgtcc
      961 ataatgaact atttcaggag tattggagta tgtggattca aaacagtggc taattgagtc
     1021 aggattactt aatattcgac tcctaccctc agaatgttgt tctccaactt caagaaactg
     1081 ttacccatga aagacccccg ctgacgggta gtcaatcact cagaggagac cctcccaagg
     1141 aacagcgaga ccacaagtcg gatgcaactg caagagggtt tattggatac acgggtaccc
     1201 gggcgacgca gtctatcgga ggactggcgc gccgagtgag gggttgtggg ctcttttatt
     1261 gagctcgggg agcagaagcg cgcgaacaga agcgagaagc gaactgattg gttagttcaa
     1321 ataaggcaca gggtcatttc aggtccttgg ggcaccctgg aaacatctga tggttctcta
     1381 gaaactgctg agggcgggac cgcatctggg gaccatctgt tcttggccct gagccggggc
     1441 aggaactgct taccacagat atcctgtttg gcccatattc tgctgtctct ctgttcctaa
     1501 ccttgatctg aacttctcta ttctcagtta tgtattttcc atgccttgca aaatggcgtt
     1561 acttaagcta gcttgccaaa cctacaggtg gggtctttca ttcccccctt tttctggaga
     1621 ctaaataaaa tcttttattt tatcgataag cttggctgca ggtcgacggt atcgataagc
     1681 ttgataattc ttaattaatt tacttgtaca gctcgtccat gccgccggtg gagtggcggc
     1741 cctcggcgcg ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
     1801 acttgatgtt gacgttgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg
     1861 tcttgacctc agcgtcgtag tggccgccgt ccttcagctt cagcctctgc ttgatctcgc
     1921 ccttcagggc gccgtcctcg gggtacatcc gctcggagga ggcctcccag cccatggttt
     1981 tcttctgcat tacggggccg tctgagggga agttggtgcc gcgcagcttc accttgtaga
     2041 tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga
     2101 agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcaag tagtcgggga
     2161 tgtcggcggg gtgcttcacg taggccttgg agccgtacat gaactgaggg gacaggatgt
     2221 cccaggcgaa gggcaggggg ccacccttgg tcaccttcag cttggcggtc tgggtgccct
     2281 cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct
     2341 ccatgtgcac cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcgccct
     2401 tgctcaccat ggttgtggcc atattatcat cgtgtttttc aaaggaaaac cacgtccccg
     2461 tggttcgggg ggcctagacg tttttttaac ctcgactaaa cacatgtaaa gcatgtgcac
     2521 cgaggcccca gatcagatcc catacaatgg ggtaccttct gggcatcctt cagccccttg
     2581 ttgaatacgc ttgaggagag ccatttgact ctttccacaa ctatccaact cacaacgtgg
     2641 cactggggtt gtgccgcctt tgcaggtgta tcttatacac gtggcttttg gccgcagagg
     2701 cacctgtcgc caggtggggg gttccgctgc ctgcaaaggg tcgctacaga cgttgtttgt
     2761 cttcaagaag cttccagagg aactgcttcc ttcacgacat tcaacagacc ttgcattcct
     2821 ttggcgagag gggaaagacc cctaggaatg ctcgtcaaga agacagggcc aggtttccgg
     2881 gccctcacat tgccaaaaga cggcaatatg gtggaaaata acatatagac aaacgcacac
     2941 cggccttatt ccaagcggct tcggccagta acgttagggg gggggggcgg aattcttagt
     3001 catagtagct tagctgaact gggccgtggg ggtcgtcatc atctccaccg gaaccagaag
     3061 aacccaaaag cagcgtagga aggtgtggat caccgccgcc atggccggaa tcatgactat
     3121 gaccgccgcc tccgtctgtc atcaaaggcg ggccctggtc acctcctttg ttttcaacct
     3181 cttccgtcaa ttgtggaggg cctccatcat ttccagcaga gtcgctaggg ctatgaggca
     3241 gcgggtcatg tgggccattg tcatcagtgt tgtcagggtc ctgtgggcca ttgtcatcag
     3301 tgttgtcagg gtcctgaggc agcgggtcat gtgggccatt gtcatcagtg ttgtcagggt
     3361 cctgtgggcc attgtcatca gtgttgtcag ggtcctgtgg gccattgtca ggaccacctc
     3421 caggtgcgcc taggttttga gagcagagtg ggggtccgtc gccggctcca ctcacgagca
     3481 ggtggtgtct gccctcgttg gagttagagt cagattcatg gccagaatca tcggtagctt
     3541 gttgagggtg cgggagggag tcatcgtggt ggtgttcatc actgtgtcgt tgtccatggt
     3601 aatacatcca gattaaaatc gccagaaaca ggaggagcca aaggagatca accaatagag
     3661 tccaccagtt ttgttgtaga tagagagcaa taatgagcag gatgaggtct aggaagaagg
     3721 ctaggaagaa ggccaaaagc tgccagatgg tggcaccaag tcgccagagc atctccaata
     3781 agtagatcca gatacctaag acaagtaagc acccgaagat gaacagcaca attccaagga
     3841 acaatgcctg tccgtgcaaa ttccagagag cgatgagcag gagggtgatc atcagtagga
     3901 gtatacaaag ggctccaagt ggacagagaa ggtctcttcg gaagataaag atgatcaaaa
     3961 ttataattat aagcatgaga gcaaaggaat agaggacaag gagggctcct ccagtccagt
     4021 cactcataac gatgtacagc caaaacagta gcgccaagag gaggagaagg agagcaaggc
     4081 ctagggaaga ggagaggggg ggtcctcgag ggggccgtcg cgggcccggt gggcccctct
     4141 caaggtcgtg ttccatggtg gccagatcta attccggcgc ctagagaagg agtgagggct
     4201 ggataaaggg aggatcgagg cggggtcgaa cgaggaggtt caagggggag agacggggcg
     4261 gatggaggaa gaggaggcgg aggcttaggg tgtacaaagg gcttgaccca gggagggggg
     4321 tcaaaagcca aggcttccca ggtcacgatg taggggacct ggtctgggtg tccatgcggg
     4381 ccaggtgaaa agaccttgat cttaacctgg gtgatgaggt ctcggttaaa ggtgccgtct
     4441 cgcggccatc cgacgttaaa ggttggccat tctgcagagc agaaggtaac ccaacgtctc
     4501 ttcttgacat ctaccgactg gttgtgagcg atccgctcga catctttcca gtgacctaag
     4561 gtcaaactta agggagtggt aacagtctgg ccctaatttt cagacaaata cagaaacaca
     4621 gtcagacaga gacaacacag aacgatgctg cagcgctgca gcagacaaga cgcgcggctt
     4681 cggttccaaa ccgaaagcaa aaattcagac ggaggcggga actgttttag gttctcgtct
     4741 cctaccagaa ccacatatcc tgacggggtc ggattccaca tcgactccct tcctcaggtc
     4801 gggccacaaa aacggccccc aaagtccctg ggacgtctcc cagggttgcg gccgggtgtt
     4861 cagaactcgt cagttccacc acgggtccgc cagatacaga gctagttagc taactagtac
     4921 agacgcaggc gcaaacatta gatgccggca caaacacgca caaagacaga gacagacacg
     4981 aaacgaccgc tggccagctt acctcccggc gggggggtcg gtggtccctg ggcaggggtc
     5041 tccaaatctc ggtggaacct ccaaatgaaa gacccccgag gtgggcagtc aatcaatctg
     5101 aggagaccct cccaaggatc agcgagtcca cgattcggat gcaaacagca agaggcttta
     5161 ttgggaatac gggtacccgg gcgacgcagt ctatcggagg actggcgcgc cgagtgaggg
     5221 gttgtgggct cttttattga gctcggggag cagaagcgcg cgaacagaag cgagaagcga
     5281 actgattggt tagttcaaat aaggcacagg gtcatttcag gtccttgggg caccctggaa
     5341 acatctgatg gttctctaga aactgctgag ggcgggaccg catctgggga ccatctgttc
     5401 ttggccctga gccggggcag gaactgctta ccacagatat cctgtttggc ccatattctg
     5461 ctgtctctct gttcctaacc ttgatctgaa cttctctatt ctcagttatg tattttccat
     5521 gccttgcaaa atggcgttac ttaagctagc ttgccaaacc tacaggtggg gtctttcatt
     5581 cccccctttt tctggagact aaataaaatc ttttatttta tctatggctc gtactctata
     5641 ggcttcagct ggtgatattg ttgagtcaaa actagagcct ggaccactga tatcctgtct
     5701 ttaacaaatt ggactaatcg cctctacgcc ggacgcatcg tggccggcat caccggcgcc
     5761 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
     5821 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
     5881 ggactgttgg gcgccatctc cttgcatgca ccattccttg cgccgtcgtt ttacaacgtc
     5941 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     6001 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     6061 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     6121 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     6181 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     6241 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     6301 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     6361 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6421 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6481 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6541 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6601 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6661 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6721 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6781 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6841 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6901 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6961 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7021 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7081 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7141 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7201 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7261 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7321 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7381 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7441 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7501 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7561 tgcgcgtaat ctgctgcttg caaac
//
