LOCUS       MSCV-opEBNA2-imC                 7899 bp    DNA     circular SYN 28-MAR-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(1703..2410)
                     /note="pLannotate"
                     /label="mCherry"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     LTR             complement(5378..5894)
                     /note="pLannotate"
                     /label="5' LTR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     LTR             complement(1087..1601)
                     /note="pLannotate"
                     /label="3' LTR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     misc_feature    complement(2424..2886)
                     /note="pLannotate"
                     /label="IRES"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             complement(4490..4906)
                     /note="pLannotate"
                     /label="gag (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(3011..4471)
                     /note="pLannotate"
                     /label="EBNA2"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    complement(4973..5314)
                     /note="pLannotate"
                     /label="MESV Ψ"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        6722..6826
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             6827..7687
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      join(7858..7899,1..547)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        871..901
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    835..856
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    909..925
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             6236..6385
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="86.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(6391..6490)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="70.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             6035..6236
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="17.0"
                     /fragment="True"
                     /other="CDS"
     CDS             730..822
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(6592..6627)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(1660..1686)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="25.0"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(5936..6034)
                     /note="pLannotate"
                     /label="env (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.0"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      721 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      781 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      841 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      901 gtgtggaatt gtgagcggat aacaatttca caattctccc cagctctttc tgcgctgtcc
      961 ataatgaact atttcaggag tattggagta tgtggattca aaacagtggc taattgagtc
     1021 aggattactt aatattcgac tcctaccctc agaatgttgt tctccaactt caagaaactg
     1081 ttacccatga aagacccccg ctgacgggta gtcaatcact cagaggagac cctcccaagg
     1141 aacagcgaga ccacaagtcg gatgcaactg caagagggtt tattggatac acgggtaccc
     1201 gggcgacgca gtctatcgga ggactggcgc gccgagtgag gggttgtggg ctcttttatt
     1261 gagctcgggg agcagaagcg cgcgaacaga agcgagaagc gaactgattg gttagttcaa
     1321 ataaggcaca gggtcatttc aggtccttgg ggcaccctgg aaacatctga tggttctcta
     1381 gaaactgctg agggcgggac cgcatctggg gaccatctgt tcttggccct gagccggggc
     1441 aggaactgct taccacagat atcctgtttg gcccatattc tgctgtctct ctgttcctaa
     1501 ccttgatctg aacttctcta ttctcagtta tgtattttcc atgccttgca aaatggcgtt
     1561 acttaagcta gcttgccaaa cctacaggtg gggtctttca ttcccccctt tttctggaga
     1621 ctaaataaaa tcttttattt tatcgataag cttggctgca ggtcgacggt atcgataagc
     1681 ttgataattc ttaattaatt tacttgtaca gctcgtccat gccgccggtg gagtggcggc
     1741 cctcggcgcg ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
     1801 acttgatgtt gacgttgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg
     1861 tcttgacctc agcgtcgtag tggccgccgt ccttcagctt cagcctctgc ttgatctcgc
     1921 ccttcagggc gccgtcctcg gggtacatcc gctcggagga ggcctcccag cccatggttt
     1981 tcttctgcat tacggggccg tctgagggga agttggtgcc gcgcagcttc accttgtaga
     2041 tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga
     2101 agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcaag tagtcgggga
     2161 tgtcggcggg gtgcttcacg taggccttgg agccgtacat gaactgaggg gacaggatgt
     2221 cccaggcgaa gggcaggggg ccacccttgg tcaccttcag cttggcggtc tgggtgccct
     2281 cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct
     2341 ccatgtgcac cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcgccct
     2401 tgctcaccat ggttgtggcc atattatcat cgtgtttttc aaaggaaaac cacgtccccg
     2461 tggttcgggg ggcctagacg tttttttaac ctcgactaaa cacatgtaaa gcatgtgcac
     2521 cgaggcccca gatcagatcc catacaatgg ggtaccttct gggcatcctt cagccccttg
     2581 ttgaatacgc ttgaggagag ccatttgact ctttccacaa ctatccaact cacaacgtgg
     2641 cactggggtt gtgccgcctt tgcaggtgta tcttatacac gtggcttttg gccgcagagg
     2701 cacctgtcgc caggtggggg gttccgctgc ctgcaaaggg tcgctacaga cgttgtttgt
     2761 cttcaagaag cttccagagg aactgcttcc ttcacgacat tcaacagacc ttgcattcct
     2821 ttggcgagag gggaaagacc cctaggaatg ctcgtcaaga agacagggcc aggtttccgg
     2881 gccctcacat tgccaaaaga cggcaatatg gtggaaaata acatatagac aaacgcacac
     2941 cggccttatt ccaagcggct tcggccagta acgttagggg gggggggcgg aattcgttaa
     3001 cctcgagtta ttggatgcta ggtctgggcc tcttgctagg tccttccacg taatcctcgt
     3061 cgctgctggg agactcggtt gtctcgaaga tgtaatccca gctctcgtcc aggtcggcag
     3121 ggtcgatgct aggaggatac cagtcgtcag gaaacaggat aggagcctcg gggctattgt
     3181 ggctctcagg ctcgtggatt ggggacacgt taggtgtggc ggtgtgagag tttctgcaca
     3241 caggagcggc gttagaaggt ccaggagtag gagaatcgcc ggcaccttgt ccttgatgca
     3301 gtcccagcac agggctcagc tcaggcatgc tagggctaga tgtgtttggc tcaggcctcc
     3361 aagggccgcc aggttttctc tgcttgtctc tgctcttgcc cttgcctcta cctcttccac
     3421 gtccacggcc tctaccacgg gactgtcctc tgctctgtcc ctgggtctta gaaggctgtg
     3481 gagggtcaca gattggaggc caccatggag gtccagatgg cagatggtgc agctgctggt
     3541 cattgatcac gccaggagga ggttgagctg gagcagcagg aggtggcaac tgactaggtg
     3601 gcagtggcat aggagggatg ttgtagaaca cggtggggct tctaggctca gggctgtcag
     3661 ggtcgttagg ggtgctctgg tgtgtcagag gatgcataga ctggtcgggc acgtgcagca
     3721 ctggcagatg cattctaggt ggagatggtg tgggctgcag ttctgtaggc ctctgcagca
     3781 cagtcagcag aggtgttgga ggaagtgtgg taggcctggt tggtcttgga ggcactgtca
     3841 gtgtagctgg aggcagtggt gtaggaggtg taggcctagg aggcatcatc agaggctgag
     3901 gaggtgtcag tctaggctgg ctcagtggag acaggatggt aggagcggtt ggtctgagtg
     3961 gaggcacatg aggctgcacc agtctagcct gtggagctgt ctgaggtccc tgaggcagaa
     4021 tcaggcctct gtcgcctcta gactgccgca cgatgtagtt ggccatccac agcattctca
     4081 tggcagaggc cagaggtccg tgtccgacat cgtatcccag gggatctcta tccagaggac
     4141 tgggctcttg tgtccaggca tctcttcttt gaggcggtgg tggtggtgga ggtggcggcg
     4201 gtggagaagg tggtggcggc ggaggcggtg gcggtggtgg cggaggtgga ggcggaggtg
     4261 gtggaggcgg cggtggcggt ggaggcaatg gaggtggaac gcctgtgttc tcgcccacga
     4321 agattgtcag agggatcaga ggtgtgtcgc tcagctgctc ttggtagggg ttgctaggga
     4381 tcacgctcag gctagggttg cccaggctgt ctgtatccac gatcaggtgg taggtctgtc
     4441 cgccgtgcag agccagatag aaggtgggca tggtggcaga tctaattccg gcgcctagag
     4501 aaggagtgag ggctggataa agggaggatc gaggcggggt cgaacgagga ggttcaaggg
     4561 ggagagacgg ggcggatgga ggaagaggag gcggaggctt agggtgtaca aagggcttga
     4621 cccagggagg ggggtcaaaa gccaaggctt cccaggtcac gatgtagggg acctggtctg
     4681 ggtgtccatg cgggccaggt gaaaagacct tgatcttaac ctgggtgatg aggtctcggt
     4741 taaaggtgcc gtctcgcggc catccgacgt taaaggttgg ccattctgca gagcagaagg
     4801 taacccaacg tctcttcttg acatctaccg actggttgtg agcgatccgc tcgacatctt
     4861 tccagtgacc taaggtcaaa cttaagggag tggtaacagt ctggccctaa ttttcagaca
     4921 aatacagaaa cacagtcaga cagagacaac acagaacgat gctgcagcgc tgcagcagac
     4981 aagacgcgcg gcttcggttc caaaccgaaa gcaaaaattc agacggaggc gggaactgtt
     5041 ttaggttctc gtctcctacc agaaccacat atcctgacgg ggtcggattc cacatcgact
     5101 cccttcctca ggtcgggcca caaaaacggc ccccaaagtc cctgggacgt ctcccagggt
     5161 tgcggccggg tgttcagaac tcgtcagttc caccacgggt ccgccagata cagagctagt
     5221 tagctaacta gtacagacgc aggcgcaaac attagatgcc ggcacaaaca cgcacaaaga
     5281 cagagacaga cacgaaacga ccgctggcca gcttacctcc cggcgggggg gtcggtggtc
     5341 cctgggcagg ggtctccaaa tctcggtgga acctccaaat gaaagacccc cgaggtgggc
     5401 agtcaatcaa tctgaggaga ccctcccaag gatcagcgag tccacgattc ggatgcaaac
     5461 agcaagaggc tttattggga atacgggtac ccgggcgacg cagtctatcg gaggactggc
     5521 gcgccgagtg aggggttgtg ggctctttta ttgagctcgg ggagcagaag cgcgcgaaca
     5581 gaagcgagaa gcgaactgat tggttagttc aaataaggca cagggtcatt tcaggtcctt
     5641 ggggcaccct ggaaacatct gatggttctc tagaaactgc tgagggcggg accgcatctg
     5701 gggaccatct gttcttggcc ctgagccggg gcaggaactg cttaccacag atatcctgtt
     5761 tggcccatat tctgctgtct ctctgttcct aaccttgatc tgaacttctc tattctcagt
     5821 tatgtatttt ccatgccttg caaaatggcg ttacttaagc tagcttgcca aacctacagg
     5881 tggggtcttt cattcccccc tttttctgga gactaaataa aatcttttat tttatctatg
     5941 gctcgtactc tataggcttc agctggtgat attgttgagt caaaactaga gcctggacca
     6001 ctgatatcct gtctttaaca aattggacta atcgcctcta cgccggacgc atcgtggccg
     6061 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     6121 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     6181 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcgccgt
     6241 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6301 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6361 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
     6421 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     6481 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     6541 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     6601 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     6661 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     6721 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6781 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6841 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6901 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6961 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7021 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7081 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7141 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7201 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7261 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7321 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7381 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7441 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7501 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7561 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7621 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7681 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7741 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7801 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7861 agatcctttt tttctgcgcg taatctgctg cttgcaaac
//
