LOCUS       pCAG-2dV-Camui(T        8415 bp DNA     circular SYN 25-APR-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 25, 2024 from SnapGene 7.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        8..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        389..667
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      1759..1768
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             join(1765..1767,1768..1770,1771..2481)
                     /codon_start=1
                     /product="=REACh (Lee 2009)
                     =Venus(A206K,T145W)"
                     /label=mVenus(Y145W)
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 1765 .. 1767 / #e1ff2f
                        2: 1768 .. 1770 / #e1ff2f / 1a
                        3: 1771 .. 2481 / #e1ff2f"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNWNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      2500..2509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             join(2506..2508,2509..2511,2512..3222)
                     /codon_start=1
                     /product="=REACh (Lee 2009)
                     =Venus(A206K,T145W)"
                     /label=mVenus(Y145W)
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 2506 .. 2508 / #ccffcc
                        2: 2509 .. 2511 / #ccffcc / 1a
                        3: 2512 .. 3222 / #ccffcc"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNWNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3244..4677
                     /codon_start=1
                     /label=CaMKII-alpha (T286A)
                     /note="CaMKII-alpha (wt)"
                     /note="color: #808080"
                     /translation="MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIIN
                     TKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAR
                     EYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEV
                     EGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLY
                     QQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCM
                     HRQEAVDCLKKFNARRKLKGAILTTMLATRNFSGGKSGGNKKNDGVKESSESTNTTIED
                     EDTKVRKQEIIKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYF
                     ENLWSRNSKPVHTTILNPHIHLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRR
                     DGKWQIVHFHRSGAPSVLPH"
     regulatory      4696..4705
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             join(4702..4704,4705..4707,4708..5418)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP(A206K)
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 4702 .. 4704 / #05fd14
                        2: 4705 .. 4707 / #05fd14 / 1a
                        3: 4708 .. 5418 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    5502..5557
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
                     /note="color: #a6acb3"
     primer_bind     complement(5918..5934)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    5942..5958
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(join(5966..5972,5973..5990,5991..5996))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 5966 .. 5972 / #ffffff / -10
                        2: 5973 .. 5990 / #ffffff
                        3: 5991 .. 5996 / #ffffff / -35"
     protein_bind    6011..6032
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        6090..6286
                     /label=SV40 promoter
                     /note="SV40 early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      6137..6272
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     polyA_signal    6292..6426
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(6665..7253)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(join(7424..8215,8216..8284))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7424 .. 8215 / #ccffcc
                        2: 8216 .. 8284 / #ccffcc / signal sequence
                     Cleavage site after base 8215"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8285..8389)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gtcgatcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
      421 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
      481 gcagcgatgg gggcgggggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga
      541 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
      601 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
      661 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
      721 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
      781 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
      841 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
      901 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
      961 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     1021 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     1081 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     1141 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     1201 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     1261 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag
     1321 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     1381 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
     1441 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
     1501 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     1561 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     1621 gcgtgtgacc ggcggctcta gctagagcct ctgctaacca tgttcatgcc ttcttctttt
     1681 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
     1741 tcgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     1801 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1861 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc
     1921 aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct gcagtgcttc
     1981 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     2041 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     2101 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     2161 gaggacggca acatcctggg gcacaagctg gagtacaact ggaacagcca caacgtctat
     2221 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     2281 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     2341 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag caaagacccc
     2401 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     2461 ggcatggacg agctgtacaa gtccggagcg ctacaggtcg ccaccatggt gagcaagggc
     2521 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     2581 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     2641 aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     2701 ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     2761 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     2821 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     2881 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     2941 tggaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     3001 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag
     3061 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     3121 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     3181 accgccgccg ggatcactct cggcatggac gagctgtaca agtctggtct cagatctcga
     3241 gctatggcta ccatcacctg cacccgattc acggaagagt accagctctt cgaggaactg
     3301 ggaaagggag ccttctccgt ggtgcgcagg tgtgtgaagg tgctggctgg ccaggagtat
     3361 gctgccaaga ttatcaacac caagaagctc tcagccagag atcaccagaa gttggaacgc
     3421 gaggcccgca tctgccgctt gttgaagcac cccaatatcg tccgactcca tgacagcatc
     3481 tccgaggagg ggcaccacta ccttatcttc gatctggtca ctggtgggga gctgttcgaa
     3541 gacattgtgg cccgggagta ttacagtgag gctgatgcca gccactgtat ccagcagatc
     3601 ctggaggctg tgctacactg tcaccagatg ggggtggtgc atcgcgacct gaagcctgag
     3661 aatctgttgc tggcttcgaa gctcaagggt gctgcggtga agctggcaga ctttggcctg
     3721 gccatagagg ttgagggaga gcagcaggca tggtttgggt tcgcagggac acctggatac
     3781 ctctccccag aagtgctgcg gaaggaccca tacgggaagc ctgtggacct gtgggcctgt
     3841 ggcgtcatcc tgtatatctt gctggttggg tatcccccat tctgggatga ggaccagcac
     3901 cgcctgtacc agcagatcaa agctggtgcc tacgatttcc catcaccaga atgggacacc
     3961 gtcaccccgg aagccaagga tctgatcaat aagatgctga ccatcaaccc gtccaaacgc
     4021 atcacggccg ctgaggctct caagcacccc tggatctcgc accgctccac tgtggcctcc
     4081 tgcatgcaca gacaggaggc cgtggactgc ctgaagaagt tcaatgccag gaggaaactg
     4141 aagggagcca tcctcaccac tatgctggcc accaggaact tctccggagg gaagagtgga
     4201 ggaaacaaga agaatgatgg cgtgaaggaa tcctctgaga gcaccaacac caccatcgag
     4261 gatgaagaca ccaaagtgcg caaacaggaa attatcaaag tgacagagca gctgatcgaa
     4321 gccataagca atggagactt tgaatcctac acgaagatgt gcgaccctgg aatgacagcc
     4381 tttgaaccgg aggccctggg gaacctggtc gagggcctgg actttcatcg attctatttt
     4441 gaaaacctgt ggtcccggaa cagcaagccc gtgcacacca ccatcctgaa ccctcacatc
     4501 cacctgatgg gtgacgagtc agcctgcatc gcctacatcc gcatcactca gtacctggat
     4561 gcgggtggca tcccccgcac ggcccagtca gaggagaccc gtgtctggca ccgcagggat
     4621 ggaaaatggc agatcgtcca cttccacaga tctggggcgc cctccgtcct gccccatgga
     4681 ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     4741 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     4801 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     4861 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     4921 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     4981 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     5041 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     5101 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     5161 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     5221 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     5281 gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac
     5341 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     5401 atggacgagc tgtacaagta gtctagagtc gacggatctt tttccctctg ccaaaaatta
     5461 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     5521 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     5581 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
     5641 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     5701 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     5761 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     5821 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
     5881 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     5941 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6001 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6061 agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc
     6121 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6181 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6241 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt
     6301 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6361 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6421 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6481 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6541 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6601 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6661 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6721 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6781 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6841 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6901 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6961 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7021 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7081 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7141 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7201 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7261 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7321 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7381 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7441 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7501 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7561 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7621 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7681 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7741 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7801 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7861 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7921 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7981 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8041 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8101 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8161 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8221 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8281 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8341 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8401 gaaaagtgcc acctg
//
