LOCUS       Exported               17162 bp ds-DNA     circular SYN 26-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pArgB-hA3A-nCas9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17162)
  AUTHORS   Fanglong Zhao
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 29, 2024 from SnapGene 4.3.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17162
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..2249
                     /label=ArgB
     primer_bind     1..46
                     /label=Primer 2
     misc_feature    1..23
                     /label=Feature 33
     misc_feature    24..2218
                     /label=Feature 2
     CDS             717..1835
                     /codon_start=1
                     /label=ArgB Af
                     /translation="MTCGLKLAAARYGNHTLRQRIPLNAVRRYTSHTATSTTPPTSPFA
                     PRHFLSIADLTSTEFATLVRNASSHKRTIKSGSIPQNLLGSMTGQTVAMLFSKRSTRTR
                     ISTEGAVVRLGGHPMFLGKDDIQLGVNESLYDSAVVISSMVSCIVARVGKHAEVADLAK
                     HSTVPVINALCDSFHPLQAIADFQTIYETFTPKAHRSDSLGLEGLKIAWVGDANNVLFD
                     MAIAATKMGIDIAVATPKGYEIPAPMLELIKQASNGVSKPGKIIETNVPEEAVKGADIL
                     VTDTWVSMGQEAESIKRVKDFEGFQITSELAKRGGANEGWKFMHCLPRHPEEVSDEVFY
                     SPRSLVFPEAENRLWAAISAMEGFVVNKGRIE"
     primer_bind     complement(2194..2249)
                     /label=Primer 3
     primer_bind     2219..2277
                     /label=Primer 5
     misc_feature    2219..2249
                     /label=Feature 32
     misc_feature    2250..4154
                     /label=Feature 25
     rep_origin      complement(2324..2912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3083..3943)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3944..4048)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4135..4192)
                     /label=Primer 6
     misc_feature    4155..6431
                     /label=AMA1 ori for Aspergillus
     misc_feature    6432..6785
                     /label=AMA1 ori for Aspergillus
                     /label=AMA1 ori for Aspergillus(2)
     primer_bind     7117..7143
                     /label=Primer 9
     misc_feature    7529..8404
                     /label=An Tef1
     primer_bind     complement(7729..7750)
                     /label=v1
     primer_bind     8127..8148
                     /label=v2
     misc_feature    8405..14026
                     /label=hA3A-nCas9
     misc_feature    8405..8412
                     /label=notI
     misc_feature    8413..8415
                     /label=ATG
     misc_feature    8416..8448
                     /label=NLS
     misc_feature    8449..8697
                     /label=UGI
     misc_feature    8698..8727
                     /label=linker
     misc_feature    8728..9321
                     /label=hA3A (Y130F)
     misc_feature    9112..9114
                     /label=Y130F
     misc_feature    9322..9417
                     /label=linker
     primer_bind     9413..9436
                     /label=Primer 7
     CDS             9418..13518
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=nCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    9442..9444
                     /label=D10A
     primer_bind     complement(9481..9501)
                     /label=Primer 1
     primer_bind     complement(9625..9646)
                     /label=v3
     primer_bind     13412..13434
                     /label=v4
     misc_feature    13543..14026
                     /label=terminator
     primer_bind     complement(14006..14064)
                     /label=Primer 4
     misc_feature    14027..17154
                     /label=Feature 18
     primer_bind     complement(14058..14072)
                     /label=Primer 5
     misc_feature    14335..14364
                     /label=lac prom
     misc_feature    14397..14417
                     /label=M13_rev
     misc_feature    14430..15590
                     /label=2micron2
     misc_feature    16031..16255
                     /label=URA3 prom
     CDS             16257..17060
                     /codon_start=1
                     /gene="URA3"
                     /label=URA3
                     /note="Orf frame 3"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(join(17127..17162,1..23))
                     /label=Primer 8
     misc_feature    17155..17162
                     /label=Feature 18
ORIGIN
        1 attccgcggt tttttggggt agtcggaaga taattcgaat ggaggcgcag gactcgagtc
       61 ggaagattgt ctttcgtcga tcggtgacga aggacgcgaa gagggcctgg accccttgtt
      121 cagtaaaggc ctgcttgcct cttcagccat gatggctggg ggggccggat aagtggggga
      181 gatggggaga gggatgtgga ggagataaca cgatagcaat acgttatggg tagataagag
      241 agataaaaga tgatggcgtt cgtagattga gaaaagaaag ctcccggtgg tgatgagttc
      301 ggtagaaggg gacgtaaggc taagaattaa aatatttagc ttgcgtatgg acaacgcacg
      361 caggatgaat tggccagacc tggaagtata gcctatcagt ttgaatcacc gtgatgatcg
      421 gcaatcatac cacgctaacc cgaactggac aaggcgtggt gtggagacgg aaaaagcgga
      481 tcacattgga ggagtcacac ttaacgcttt caagctaact gactgactcg acggtcgagc
      541 taaggtacgt actatttctt ttttcttttc tctttgtctc ttgttgctgc tgttgactct
      601 ctctcttcta gtccgataag aactgagtaa tttcaatcct ctggttctta ctctatatat
      661 tccccctccc ctactaccca attgcttctc cgcatactac cggaaattat ctcatcatga
      721 cttgcggtct gaagctggcc gccgctcgtt acggcaacca taccttgcgc cagaggattc
      781 cactcaacgc tgtgcgccga tacacttctc acactgctac ttctacgact ccccccacct
      841 cgccattcgc tcctcgccat tttctttcca tcgctgatct tacctcaacg gagttcgcaa
      901 ccctcgtccg taatgcatct tcacacaaac gaaccatcaa gtcgggatca atccctcaga
      961 acctgcttgg ttctatgacc ggacagactg tagctatgct gttcagcaaa cgcagtacca
     1021 gaaccaggat atctactgaa ggggctgtgg tgcgcttggg aggacatcca atgtttctgg
     1081 gcaaggatga tattcaactc ggtgtcaatg agtccttgta tgactctgcg gttgtaatct
     1141 cctccatggt ctcttgtatt gtagcccgag tcggcaagca cgctgaggtt gcagaccttg
     1201 ccaagcattc aaccgtgcct gttatcaacg ctctctgtga ctccttccac cctctccagg
     1261 ccattgccga ttttcagacc atctacgaaa catttacacc taaggcccat cgctctgaca
     1321 gtttgggtct ggagggcctc aagattgcct gggtcggtga tgcaaacaac gttctgttcg
     1381 acatggcaat tgctgctacc aagatgggta ttgatatcgc cgttgcgact ccgaagggct
     1441 atgagattcc tgccccgatg ttggagctca tcaagcaggc cagcaacggt gtctcaaagc
     1501 caggaaagat tattgagacc aacgttcctg aggaggcggt taagggtgcg gatatcctgg
     1561 taactgacac ctgggtttcc atgggacagg aggcagagtc gatcaagcgg gtgaaggact
     1621 tcgagggctt ccagatcact tcggaacttg ccaagcgcgg tggtgcgaac gaaggctgga
     1681 agttcatgca ctgcctgcct cgtcaccctg aggaagtcag cgatgaagtt ttctacagcc
     1741 cacggtcctt ggtcttcccc gaggcggaga atcggttgtg ggctgcgatc tcggccatgg
     1801 agggctttgt tgtcaataag ggaaggattg agtaagcctt aaaaaagaaa taaatcataa
     1861 aaatctatat agtccaaagc tcaattctga atagattaat ggaccgttgt ttgactttcc
     1921 ataccgaagt atgtagtata gaataccaca aacatgtaaa cacgtctata tatgtagaag
     1981 taaaaccgca cttactacgg aggtataaaa aaataaagcc gttggtgccc gcattcacat
     2041 gtcacgggcg gagttgccga caaaacaagg ccaacaaccc tccaaaatct catgagctgc
     2101 tgcatccatc ataccattaa tgagcttgcc actctcttag tgccctcttc ctatatttat
     2161 tgccctccgt ggccccaggc aacctagatg gaggtatgta tttccctgct gacgggtaat
     2221 tcttctgagg tttcgcaatg gctgtaggtc tctctacata tgacatgtga gcaaaaggcc
     2281 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2341 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2401 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2461 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2521 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2581 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2641 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2701 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2761 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2821 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2881 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2941 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3001 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3061 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3121 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3181 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaacca cgctcaccgg
     3241 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3301 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3361 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3421 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3481 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3541 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3601 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3661 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3721 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3781 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3841 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3901 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3961 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4021 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     4081 ctagctgtac aaaaaagcag gctttaaagg aaccaattca gtcgactgga tccggtacca
     4141 aggtcgggca ggaactgcag attttgacct agttaacaag gcacatcatg ctatcacttc
     4201 caacactctt gctgagcgcg ttctgccttg cctttctggt tctacgaagt atccagtccc
     4261 gcctgcaacg ccgcagaaac gccaaaaggt ggggttgtca accgccagct agagctccat
     4321 cgggattctt tggcattccg ccgttcctcg gtggtcgcag ataactccct aaattccctg
     4381 agctaactct aagtcgacca tgccgtttat ggttagcgcc tcccaaaaag gaatggccga
     4441 cttagagtta cctcttgacc gactttttct ttcctccccc ttacatttcg ttaccacaac
     4501 acattcctat atccaaactc caggtacata actagtcgaa atctctttta aatctagtca
     4561 agaactagat acttaacctt catactgctt agagatagcc tttgaagctc ttatttcgct
     4621 ctttgtctca ctctcacctt cctcctcctc ctccaatcct ttttgctcgg gactagtcca
     4681 agactagtca acgattagta tgccgagcat tcgcgataac gatctccgaa agtccccaga
     4741 gtactgtcac tatctcgagg cagttaagga cggggagctt acgctgccgg atttcaagat
     4801 agtaagccga cccgactagc tttaagccta gttactgact agtccacagg acgacaatgg
     4861 cgtgcctgat atccatccat atgaagtcta ctgccgagtg aagggatgcc tcaagcgtac
     4921 agtgagtcta ctgctatact agtttctgac tagttattaa ctagttgaca ggttccctct
     4981 gccaacagaa acatattggt caagcacttg aaggacaaga actcccacgg catggagttt
     5041 acattgcaca atggacctcc cactatgaag gaactgatgg aggccaaagg caagtcctat
     5101 ctagattact tggtgactag tccctgacta gtcaagtagc atggtatgaa ggcttgtttg
     5161 aaggcactgt tctcccaacc ccgactccta ccaagaagcg caagcgagct gcgtaagttt
     5221 ctgtgagtct aactagtgta ttagctaata tatagcagca ccaagtccaa ggaccacaat
     5281 actaagggtg tcgagaattc gtgagtttct tctcccattt caactagtcc ttgactagtc
     5341 actaactact ttgcagcaac gagggtgaag ctggaaatga tcaggacaat ggcgagggcc
     5401 cgtaagtaca gccattcaat gcagactagt tgctaactaa tctgtgacta gttcaagtgg
     5461 tccgtacgcc gtgcataccc ctgtgactgg taggaatttg agcaagcctg tcttgccgcg
     5521 cgatgagaaa ggaaaggcaa gttacattca gccccgtacc taggatcagt gctaatttat
     5581 aacctctagc cactctttat gcagatccgc cgtgagggta gcaaggcagc taaatcagct
     5641 ggtgagaaag gaaccatacc ctgcaagacc tgtcgcaacg caaagggcaa aggtaagcta
     5701 tccaagctag tttgggacta gattctaact agtctcagca ccgtgtggtt caaagccata
     5761 ttgcgagttt tggcgctttt tctcatcgat tgacgaggca aagggagcga gtatgcaacc
     5821 tcgtaagtca gactcagaca aagaccaact agttattgac cagtcttcta gaaggctctg
     5881 ttgtggatct tgaggccctg gagagttcct ccaacaatcc ggagacaagc aagtcctcgt
     5941 cggactagtc actaactaga ctctaactag ttgcagacat ggataatgca aaagagacaa
     6001 gcaatgaaga aagtggtaag acatttctcc tttgtggttc tggactagtc tttgactagt
     6061 cacagtctta aacaaggaaa atgagcatga aaatgaggag gaaaaggctg ctgagcccga
     6121 ggaagtgcag ggtgatggca gacatggtag gttaatacct tgttagttat tgctagtcac
     6181 tgactagtca ataactagtc tctgaacacc ttgcaatcac tccgtttgcg cagctgaaca
     6241 gtggtgagga taatagtagt aagttattct agcttcagag ttataggaga ctagatacta
     6301 actagtatta gttgcaacta acctggatct cagagacttt ggcctcaatc tagaatctat
     6361 ctagttgtca actagactgt ggtatcattg tcttttattt tcctagtcct ggaactagct
     6421 tctaactagt ctccctaata tgtggctgtc ttgttttttt tttttgtttc cctacccgga
     6481 tatctagtcc ccttctaggt tctgttaacc tctcgggctc tgatttagtt taacgcaaac
     6541 ctgagattag tttctaacta gtctctaggt tttctatcca cctttaattg taataataaa
     6601 tacaagcaac gtttatacgt caaaagcatt tataaacttt taccctaaag tagcttgctt
     6661 gtgtgtttag tttataatta gtctcttatt aatttgatgt aggtaagccc gccacaaata
     6721 tatattttta acaagatacc gtggaaaaac ttcgtgctat cacaaaacag tatacaaaaa
     6781 ataagctggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     6841 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     6901 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6961 actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata
     7021 agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     7081 cgcgaatttt aacaaaatgg ggtgacgatg agccgctctt gcatctttgt ttgtattata
     7141 ctgtctttct tgttacacat aattattcta gaatgcccca ccgttacata cgggacacag
     7201 ccatttacat atgcatgtgg attacgagct aacgagttca ttcaaatctc agaactatca
     7261 cataatcatc attcccctat cgtcaaagac cgtaagacaa atccggttca tgcactgaac
     7321 ccattcgggt agtgagtcat ttactcagca cactcgcgct gacgctcgtc gaacaccttc
     7381 aatgcctcct cggcagcctt gacaccactg agaaccatgg caccgaaggt agggcccatg
     7441 cggttaaagc catcaatttc agacagctcc ataccgccga ttatcaagcc cttagtaacc
     7501 tcgcgggtgt tcttgacgat ggcatcctcg agacagcaga atcaccgccc aagttaagcc
     7561 tttgtgctga tcatgctctc gaacgggcca agttcgggaa aagcaaagga gcgtttagtg
     7621 aggggcaatt tgactcacct cccaggcaac agatgagggg ggcaaaaaga aagaaatttt
     7681 cgtgagtcaa tatggattcc gagcatcatt ttcttgcggt ctatcttgct acgtatgttg
     7741 atcttgacgc tgtggatcaa gcaacgccac tcgctcgctc catcgcaggc tggtcgcaga
     7801 caaattaaaa ggcggcaaac tcgtacagcc gcggggttgt ccgctgcaaa gtacagagtg
     7861 ataaaagccg ccatgcgacc atcaacgcgt tgatgcccag ctttttcgat ccgagaatcc
     7921 accgtagagg cgatagcaag taaagaaaag ctaaacaaaa aaaaatttct gcccctaagc
     7981 catgaaaacg agatggggtg gagcagaacc aaggaaagag tcgcgctggg ctgccgttcc
     8041 ggaaggtgtt gtaaaggctc gacgcccaag gtgggagtct aggagaagaa tttgcatcgg
     8101 gagtggggcg ggttacccct ccatatccaa tgacagatat ctaccagcca agggtttgag
     8161 cccgcccgct tagtcgtcgt cctcgcttgc ccctccataa aaggatttcc cctccccctc
     8221 ccacaaaatt ttctttccct tcctctcctt gtccgcttca gtacgtatat cttcccttcc
     8281 ctcgcttctc tcctccatcc ttctttcatc catctcctgc taacttctct gctcagcacc
     8341 tctacgcatt actagccgta gtatctgagc acttctccct tttatattcc acaaaacata
     8401 acacgcggcc gcatgggacc taagaaaaag aggaaggtgg cggccgctac taatctgagc
     8461 gacatcattg agaaggagac tgggaaacag ctggtcattc aggagtccat cctgatgctg
     8521 cctgaggagg tggaggaagt gatcggcaac aagccagagt ctgacatcct ggtgcacacc
     8581 gcctacgacg agtccacaga tgagaatgtg atgctgctga cctctgacgc ccccgagtat
     8641 aagccttggg ccctggtcat ccaggattct aacggcgaga ataagatcaa gatgctgagc
     8701 ggaggatccg gaggatctgg aggcagcgaa gccagcccag catccgggcc cagacacttg
     8761 atggatccac acatattcac ttccaacttt aacaatggca ttggaaggca taagacctac
     8821 ctgtgctacg aagtggagcg cctggacaat ggcacctcgg tcaagatgga ccagcacagg
     8881 ggctttctac acaaccaggc taagaatctt ctctgtggct tttacggccg ccatgcggag
     8941 ctgcgcttct tggacctggt tccttctttg cagttggacc cggcccagat ctacagggtc
     9001 acttggttca tctcctggag cccctgcttc tcctggggct gtgccgggga agtgcgtgcg
     9061 ttccttcagg agaacacaca cgtgagactg cgtatcttcg ctgcccgcat cttcgattac
     9121 gaccccctat ataaggaggc actgcaaatg ctgcgggatg ctggggccca agtctccatc
     9181 atgacctacg atgaatttaa gcactgctgg gacacctttg tggaccacca gggatgtccc
     9241 ttccagccct gggatggact agatgagcac agccaagccc tgagtgggag gctgcgggcc
     9301 attctccaga atcagggaaa ctctggagga tctagcggag gatcctctgg cagcgagaca
     9361 ccaggaacaa gcgagtcagc aacaccagag agcagtggcg gcagcagcgg cggcagcgac
     9421 aagaagtata gcatcgggct ggccattgga acgaactcgg ttggttgggc tgtgattacg
     9481 gacgaataca aggtgccatc caagaagttt aaggtcctgg gaaacaccga ccgtcactca
     9541 atcaagaaga atctcattgg agccctgctc ttcgatagtg gggagaccgc cgaagctact
     9601 cgactgaagc gaacggctcg ccggcgttat acacgacgca agaatcgcat ctgctacctc
     9661 caggagattt tcagcaacga aatggctaag gttgatgact cattctttca tcgactcgaa
     9721 gaaagtttct tggtcgagga ggataagaag cacgagcgcc atccgatctt tggtaacatt
     9781 gtggatgagg ttgcctatca cgaaaagtac ccaactatct atcatcttcg taagaagctg
     9841 gtcgatagca cggacaaggc tgatttgcga cttatctacc tggcactcgc gcacatgatt
     9901 aagttccgcg gccattttct tatcgagggt gacctgaacc ccgataattc tgacgttgat
     9961 aagctcttca tccagttggt ccaaacctac aatcagctgt ttgaggaaaa ccctattaat
    10021 gcatctggcg tggacgccaa ggctatcctt tcggcgcgcc tgtctaagtc gcggcgtttg
    10081 gagaacctta tcgcacaact ccccggcgaa aagaagaacg gcctcttcgg taatttgatt
    10141 gcgttgtcac ttggtctgac tcctaacttc aagagtaatt ttgacctggc agaggatgcg
    10201 aagctccagt tgtctaagga tacgtatgat gacgatctcg acaacttgct tgcccaaatc
    10261 ggtgaccagt acgctgatct tttcctggcc gctaagaatc tctcagatgc aatcctgctc
    10321 agtgacattt tgcgggtcaa caccgagatt actaaggccc ccctgtcagc tagtatgatc
    10381 aagcggtatg atgagcacca tcaggacctc accttgctta aggccctcgt gcgtcagcaa
    10441 ttgcctgaga agtataagga aatcttcttt gaccaatcca agaacggata cgcagggtat
    10501 attgatggcg gtgcgagcca ggaggaattc tacaagttta tcaagccgat tttggagaag
    10561 atggacggca ctgaggaact gctcgtcaag ctgaatcgcg aagatttgct tcgtaagcaa
    10621 cgaacgttcg acaacggctc catcccgcac cagattcatc tgggcgagct ccacgccatc
    10681 cttcgacgcc aggaagattt ctacccattt ctgaaggaca accgtgagaa gatcgaaaag
    10741 attcttacat tccgaatccc ctactatgtg ggacctttgg cccgtgggaa ttcccgattt
    10801 gcttggatga cccgaaagag cgaggaaacc atcactccgt ggaacttcga ggaagtcgtg
    10861 gacaagggtg catccgcgca gagcttcatt gagcggatga ccaattttga taagaacctt
    10921 ccgaatgaaa aggtcctgcc aaagcattcg ctgctctacg agtatttcac cgtgtataac
    10981 gaactgacta aggtcaagta cgtgacggag ggaatgcgga agccagcctt cctctcaggg
    11041 gaacaaaaga aggctatcgt cgatttgctt tttaagacca atcgtaaagt gactgttaag
    11101 cagctgaagg aggattattt caagaagatt gaatgtttcg actccgtcga gatcagcggc
    11161 gtggaagatc gctttaacgc ttccctcggt acctaccacg acctgctcaa gatcattaag
    11221 gacaaggatt tcctcgataa cgaggaaaat gaggacatct tggaagatat tgtcctcacg
    11281 ttgacacttt ttgaggaccg cgaaatgatc gaggaacggc tcaagacata tgcccatttg
    11341 ttcgacgata aggtgatgaa gcagctgaag cggcgtcgat acaccggatg gggtcgcctt
    11401 agccggaagc tgatcaacgg cattcgagat aagcaatctg gtaagactat cttggatttc
    11461 cttaagtcgg acggcttcgc caaccgcaat tttatgcagc ttattcacga cgattccctg
    11521 acgttcaagg aggacatcca gaaggcacaa gtctcaggac aaggggattc cctgcacgag
    11581 catatcgcca acctggctgg atccccggcg atcaagaagg ggattcttca gaccgtcaag
    11641 gttgtcgacg agctggtcaa ggtgatgggc cgtcataagc cagaaaacat cgtgattgag
    11701 atggcccgag aaaatcagac cactcaaaag ggtcagaaga acagccgcga gcggatgaag
    11761 cggatcgagg aaggcattaa ggaacttggt tctcagatcc tgaaggagca ccctgttgaa
    11821 aacacacagc tccaaaatga gaagctgtat ctctactatt tgcaaaatgg acgcgacatg
    11881 tacgtcgatc aggagctcga cattaaccgg ttgtcggact acgatgttga ccatatcgtc
    11941 ccgcaatcct tccttaagga cgatagcatt gataacaagg tgctgactcg ctcagataag
    12001 aaccggggca agtccgacaa tgttccaagc gaggaagtgg ttaagaagat gaagaactac
    12061 tggcgccaat tgcttaatgc caagctcatc acacagcgca agtttgacaa cttgaccaag
    12121 gccgagcggg gagggctgag tgaactcgat aaggctggct tcatcaagcg tcaactcgtg
    12181 gagacgcgac agatcacaaa gcacgttgct cagattctgg actcccggat gaacacaaag
    12241 tacgacgaga atgataagct catccgtgaa gttaaggtca ttaccctcaa gtctaagttg
    12301 gtgtcggatt tccgcaagga cttccaattt tataaggttc gggagatcaa caattatcac
    12361 catgcacatg atgcgtacct caacgcagtc gtgggaactg cgctcatcaa gaagtatccc
    12421 aagttggagt ccgaattcgt ctacggggat tataaggttt acgacgtccg caagatgatc
    12481 gccaagagtg agcaggaaat tggcaaggcc acggctaagt atttctttta ctccaacatc
    12541 atgaatttct ttaagacgga gatcacactc gccaatggag aaatccgtaa gcgacctttg
    12601 attgagacca acggcgagac tggtgaaatc gtttgggata aggggcgcga cttcgctacc
    12661 gtgcggaagg ttctgagcat gccgcaagtc aatatcgtca agaaaaccga ggtgcagaca
    12721 ggcggtttct ctaaggaatc gattcttcca aagcgtaact ctgacaagct gatcgctcga
    12781 aagaaggatt gggaccccaa gaagtatgga gggttcgatt ctcctacagt ggcatactcg
    12841 gttctcgttg tcgcgaaggt tgagaaggga aagtctaaga agctgaagtc ggtcaaggaa
    12901 ctgctcggga tcaccattat ggagcgctcc agcttcgaaa agaatcccat cgactttctc
    12961 gaggccaagg gctataagga agtcaagaag gatcttatca ttaagctgcc taagtactct
    13021 ttgttcgagc ttgaaaacgg tcgaaagcga atgctcgcat cggcaggaga gttgcagaag
    13081 gggaatgaat tggcacttcc ctcaaagtac gtgaacttcc tgtatctcgc gtcccactac
    13141 gagaagctga agggtagccc tgaggacaac gaacagaagc aactttttgt tgagcaacac
    13201 aagcattatc tggatgagat cattgaacag atttcagagt tcagtaagcg cgtcatcctc
    13261 gccgatgcta atctcgacaa ggtgttgtcg gcctacaaca agcaccgtga caagccgatc
    13321 cgagagcagg ctgaaaatat cattcatctg ttcaccctca ctaacttggg agcaccagca
    13381 gcgttcaagt attttgatac gacaatcgac cgtaagcgat acacgtccac aaaggaggtg
    13441 cttgatgcga ccctgattca tcaatccatc actgggctct atgaaacccg tatcgacctt
    13501 agtcaactgg ggggcgaccc tcccaagaag aagcgcaagg tctgagcgga cattcgattt
    13561 atgccgttat gacttcctta aaaaagcctt tacgaatgaa agaaatggaa ttagacttgt
    13621 tatgtagttg attctacaat ggattatgat tcctgaactt caaatccgct gttcattatt
    13681 aatctcagct cttcccgtaa agccaatgtt gaaactattc gtaaatgtac ctcgttttgc
    13741 gtgtaccttg cttatcacgt gatattacat gacctggaca gagttctgcg cgaaagtcat
    13801 aacgtaaatc ccgggcggta ggtgcgtccc gggcggaagg tagttttctc gtccacccca
    13861 acgcgtttat caacctcaac tttcaacaac catcatgcca ccaaaagcgc gtaaaacaaa
    13921 gcgagatttg attgagcaag agggcaggat ccaatgcgcg attcaagaca ttaaaaatgg
    13981 aaaatttcaa aaaattgcgc ccgcagcgcg tgcatacaaa attcatttat ttaaatggtt
    14041 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
    14101 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
    14161 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
    14221 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
    14281 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
    14341 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
    14401 aaacagctat gaccatgatt acgccactga acgaagcatc tgtgcttcat tttgtagaac
    14461 aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag
    14521 aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt
    14581 aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt
    14641 tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt
    14701 tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac
    14761 tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt
    14821 ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg
    14881 actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa
    14941 aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt
    15001 gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct
    15061 ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa
    15121 tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt
    15181 agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg
    15241 atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat
    15301 tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc
    15361 ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct agagaatagg
    15421 aacttcggaa taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc
    15481 gagctgcgca catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata
    15541 tatatataca tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc
    15601 gtctatttat gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg
    15661 cggggtatcg tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct
    15721 caattggatt agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatactaag
    15781 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
    15841 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
    15901 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
    15961 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    16021 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
    16081 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
    16141 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
    16201 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
    16261 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
    16321 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
    16381 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
    16441 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
    16501 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
    16561 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
    16621 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
    16681 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
    16741 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
    16801 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
    16861 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
    16921 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
    16981 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
    17041 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
    17101 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataatttaa
    17161 at
//
