LOCUS       pET21-6xHis.RGD.P(Q54A) 7069 bp ds-DNA     circular     08-AUG-2022
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            pet21-rgd-p-q54a-x72phocl-x72-p-q54a-rgd_qxphoclxq.gb
FEATURES             Location/Qualifiers
     CDS             complement(482..673)
                     /label="rop"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             complement(1482..2564)
                     /label="lacI"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        complement(2565..2642)
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        2951..2969
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_binding    2970..2992
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    complement(2970..2994)
                     /label="lac operator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3050..4750
                     /label="IDT Insert"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             3053..4744
                     /label="Translation 3053-4744"
                     /translation="MRGSHHHHHHEFGRGDSVDGSGSGSGSGSGSGAPQMLRELQETNAALQDVRELLRQAVKEITFLKNTVMESDASGSGSGSGSGSGSLDGSPAGSPTSTEEGTSESATPESGPGTSTEPSEGSAPGSPAGSPTSTEEGTSTEPSEGSAPGTSTEPSEGSAKLEFVIPDYFKQSFPEGYSWERSMTYEDGGICIATNDITMEGDSFINKIHFQGTNFPPNGPVMQKRTVGWEASTEKMYERDGVLKGDVKMKLLLKGGGHYRGDYRTTYKVKQKPVKLPDCHFVDHRIEILSHDKDYNKVKLYEHAVAKTSTDSMDELYKGGSGGMVSKGEETITSVIKPDMKNKLRMEGNVNGHAFVIEGEGSGKPFEGIQTIDLEVKEGAPLPFAYDILTTAFHYGNRVFTKYPRGGGQLPGTSESATPESGPGSEPATSGSETPGSEPATSGSETPGSPAGSPTSTEEGTSESATPESGPGTSTEPSEGSAPLDGSGSGSGSGSGSGAPQMLRELQETNAALQDVRELLRQAVKEITFLKNTVMESDASGSGSGSGSGSGSGRGDSHHHHHH*"
     misc_feature    3110..3310
                     /label="P domain"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3221..3223
                     /label="Q54A"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3311..3535
                     /label="X72"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          3522..3566
                     /label="Phocl2-amp_F"
                     /note="sequence: GCAGCGCCAAACTGGAATTCgtgatccctgactacttcaagcaga"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3523..3541)
                     /label="Copy of PhoCl-ins_R"
                     /note="sequence: GAATTCCAGTTTGGCGCTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     CDS             3542..4267
                     /label="PhoCl 2c"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    4229..4234
                     /label="PhoCl cleavage"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(4247..4287)
                     /label="PhoCl2-amp_R"
                     /note="sequence: CCCGGCAATTGGCCGCCGCCccgtgggtacttggtgaacac"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          4275..4290
                     /label="Copy of PhoCl-ins_F"
                     /note="sequence: GCCAATTGCCGGGAAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    4283..4507
                     /label="X72"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    4508..4711
                     /label="P domain"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4619..4621
                     /label="Q54A"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      complement(4835..4882)
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     rep_origin      4919..5374
                     /label="f1 ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        5400..5504
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             5505..6365
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      6536..55
                     /label="ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
       61 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      121 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      181 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
      241 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca atggtgcact
      301 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
      361 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
      421 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
      481 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag
      541 cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt
      601 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt
      661 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac
      721 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac
      781 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca
      841 ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc
      901 agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca
      961 gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt
     1021 tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa
     1081 ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg
     1141 gggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag
     1201 tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca
     1261 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct
     1321 gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc
     1381 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg
     1441 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt
     1501 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     1561 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
     1621 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
     1681 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta
     1741 tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg
     1801 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
     1861 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
     1921 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
     1981 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
     2041 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg
     2101 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
     2161 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
     2221 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
     2281 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
     2341 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
     2401 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
     2461 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
     2521 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
     2581 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
     2641 tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag
     2701 gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa
     2761 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
     2821 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
     2881 acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat
     2941 cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta
     3001 ggatccCCCC TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC ATATGCGTGG
     3061 CAGCCACCAT CACCATCACC ATGAGTTTGG CCGCGGAGAT TCAGTAGATG GTTCGGGCTC
     3121 TGGATCAGGT TCGGGTTCGG GTAGTGGGGC CCCACAGATG CTTCGCGAGC TCCAAGAGAC
     3181 AAACGCGGCC CTGCAAGACG TACGTGAGCT TCTCCGCCAG gcaGTAAAAG AAATTACATT
     3241 TCTGAAGAAT ACTGTTATGG AAAGCGACGC CTCAGGTTCG GGTTCTGGGT CAGGCAGCGG
     3301 CTCCGGCTCA TTGGATGGGT CGCCAGCAGG TAGTCCAACG AGTACCGAGG AAGGTACCTC
     3361 AGAAAGCGCC ACTCCAGAAT CAGGTCCAGG TACAAGCACT GAGCCTTCGG AAGGGTCTGC
     3421 CCCCGGCTCC CCAGCGGGCA GTCCCACGTC AACAGAGGAA GGCACGAGTA CTGAGCCTTC
     3481 AGAAGGGTCA GCTCCAGGGA CATCAACGGA ACCTAGTGAA GGCAGCGCCA AACTGGAATT
     3541 Cgtgatccct gactacttca agcagagctt ccccgagggc tacagctggg agcgcagcat
     3601 gacctacgag gacggcggca tctgcatcgc caccaacgac atcacaatgg agggggacag
     3661 cttcatcaac aagatccact tccagggcac gaacttcccc cccaacggcc ccgtgatgca
     3721 gaagaggacc gtgggctggg aggccagcac cgagaagatg tacgagcgcg acggcgtgct
     3781 gaagggcgac gtgaagatga agctgctgct gaagggcggc ggccactatc gcggcgacta
     3841 ccgcaccacc tacaaggtca agcagaagcc cgtaaagctg cccgactgcc acttcgtgga
     3901 ccaccgcatc gagatcctga gccacgacaa ggactacaac aaggtgaagc tgtacgagca
     3961 cgccgtggcc aagacttcca ccgacagcat ggacgagctg tacaagggtg gcagcggtgg
     4021 catggtgagc aagggcgagg agaccattac aagcgtgatc aagcctgaca tgaagaacaa
     4081 gctgcgcatg gagggcaacg tgaacggcca cgccttcgtg atcgagggcg agggcagcgg
     4141 caagcccttc gagggcatcc agacgattga tttggaggtg aaggagggcg ccccgctgcc
     4201 cttcgcctac gacatcctga ccaccgcctt ccactacggc aaccgcgtgt tcaccaagta
     4261 cccacggGGC GGCGGCCAAT TGCCGGGAAC ATCAGAATCT GCTACACCAG AATCGGGCCC
     4321 TGGGTCAGAA CCGGCCACTT CGGGTTCAGA AACACCTGGC AGTGAACCTG CTACTAGTGG
     4381 CTCGGAAACC CCAGGGTCCC CAGCCGGTTC CCCTACCTCG ACAGAAGAGG GTACATCGGA
     4441 GTCCGCGACA CCTGAATCAG GGCCAGGAAC AAGCACAGAA CCGTCGGAAG GGTCTGCCCC
     4501 TCTGGATGGA AGCGGTTCAG GCTCGGGTTC GGGTAGCGGT TCGGGCGCAC CCCAGATGTT
     4561 GCGCGAGCTT CAAGAGACTA ACGCAGCCTT ACAAGACGTA CGCGAGCTGC TGCGCCAGGC
     4621 CGTGAAAGAA ATTACCTTCT TAAAGAATAC GGTTATGGAA AGTGACGCCT CAGGGAGTGG
     4681 TAGTGGTTCG GGCAGCGGGT CAGGCTCTGG CCGCGGCGAT TCACACCATC ACCATCACCA
     4741 TTAActcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga
     4801 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
     4861 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac
     4921 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4981 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5041 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5101 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     5161 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     5221 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     5281 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5341 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     5401 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5461 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5521 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5581 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5641 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5701 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5761 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5821 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5881 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5941 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6001 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     6061 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6121 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6181 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6241 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6301 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6361 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6421 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6481 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6541 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6601 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6661 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     6721 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6781 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6841 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6901 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6961 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7021 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacct
//