LOCUS       psiT-GEM(1)        8175 bp    DNA     circular     24-APR-2024
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|-15416124|
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FEATURES             Location/Qualifiers
     primer          27..43
                     /vntifkey="27"
                     /label=T7
     misc_feature    complement(6589..7449)
                     /vntifkey="21"
                     /label=Amp
     primer          3..18
                     /vntifkey="27"
                     /label=M13F
     misc_feature    72..171
                     /vntifkey="21"
                     /label=attP
     CDS             280..588
                     /vntifkey="4"
                     /label=EY-CfaN
     misc_feature    178..277
                     /vntifkey="21"
                     /label=SA
     CDS             589..642
                     /vntifkey="4"
                     /label=T2A
     CDS             643..3285
                     /vntifkey="4"
                     /label=Gal4
     CDS             3286..3339
                     /vntifkey="4"
                     /label=T2A
     CDS             3340..3453
                     /vntifkey="4"
                     /label=CfaC-CFN
     misc_feature    3455..3575
                     /vntifkey="21"
                     /label=SD
     misc_feature    3576..3609
                     /vntifkey="21"
                     /label=FRT
     misc_feature    5225..5258
                     /vntifkey="21"
                     /label=FRT
     misc_feature    3610..5224
                     /vntifkey="21"
                     /label=3XP3-RFP-SV40
     misc_feature    complement(5259..5358)
                     /vntifkey="21"
                     /label=attP
     misc_feature    53..71
                     /vntifkey="21"
                     /label=MCS
     misc_feature    5359..5378
                     /vntifkey="21"
                     /label=MCS
BASE COUNT     2275 a      1934 c      1834 g      2132 t 
ORIGIN
        1 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgacc caccggtgca 
       61 tgcgcggccg ccccaggtca gaagcggttt tcgggagtag tgccccaact ggggtaacct 
      121 ttgagttctc tcagttgggg gcgtagggtc gccgacatga cacaaggggt ttctagaagt 
      181 cgatccaaca tggcgacttg tcccatcccc ggcatgttta aatatactaa ttattcttga 
      241 actaatttta atcaaccgat ttatctctct tccgcaggtg aatattgcct gtcctacgac 
      301 accgagatcc tgaccgtgga gtacggcttc ctgcccatcg gcaagatcgt ggaggagcgc 
      361 atcgagtgca ccgtgtacac cgtggacaag aacggcttcg tgtacaccca gcccatcgcc 
      421 cagtggcaca accgcggcga gcaggaggtg ttcgagtact gcctggagga cggctccatc 
      481 atccgcgcca ccaaggacca caagttcatg accaccgacg gccagatgct gcccatcgac 
      541 gagatcttcg agcgcggcct ggacctgaag caggtggacg gcctgcccga gggccgcggc 
      601 agcctgctga cctgcggcga tgtggaggag aaccccgggc ccatgaagct actgtcttct 
      661 atcgaacaag cctgcgatat ttgccgactt aaaaagctca agtgctccaa agaaaaaccg 
      721 aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct actctcccaa aaccaaaagg 
      781 tctccgctga ctagggcaca tctgacagaa gtggaatcaa ggctagaaag actggaacag 
      841 ctatttctac tgatttttcc tcgagaagac cttgacatga ttttgaaaat ggattcttta 
      901 caggatataa aagcattgtt aacaggatta tttgtacaag ataatgtgaa taaagatgcc 
      961 gtcacagata gattggcttc agtggagact gatatgcctc taacattgag acagcataga 
     1021 ataagtgcga catcatcatc ggaagagagt agtaacaaag gtcaaagaca gttgactgta 
     1081 tcgattgact cggcagctca tcatgataac tccacaattc cgttggattt tatgcccagg 
     1141 gatgctcttc atggatttga ttggtctgaa gaggatgaca tgtcggatgg cttgcccttc 
     1201 ctgaaaacgg accccaacaa taatgggttc tttggcgacg gttctctctt atgtattctt 
     1261 cgatctattg gctttaaacc ggaaaattac acgaactcta acgttaacag gctcccgacc 
     1321 atgattacgg atagatacac gttggcttct agatccacaa catcccgttt acttcaaagt 
     1381 tatctcaata attttcaccc ctactgccct atcgtgcact caccgacgct aatgatgttg 
     1441 tataataacc agattgaaat cgcgtcgaag gatcaatggc aaatcctttt taactgcata 
     1501 ttagccattg gagcctggtg tatagagggg gaatctactg atatagatgt tttttactat 
     1561 caaaatgcta aatctcattt gacgagcaag gtcttcgagt caggttccat aattttggtg 
     1621 acagccctac atcttctgtc gcgatataca cagtggaggc agaaaacaaa tactagctat 
     1681 aattttcaca gcttttccat aagaatggcc atatcattgg gcttgaatag ggacctcccc 
     1741 tcgtccttca gtgatagcag cattctggaa caaagacgcc gaatttggtg gtctgtctac 
     1801 tcttgggaga tccaattgtc cctgctttat ggtcgatcca tccagctttc tcagaataca 
     1861 atctccttcc cttcttctgt cgacgatgtg cagcgtacca caacaggtcc caccatatat 
     1921 catggcatca ttgaaacagc aaggctctta caagttttca caaaaatcta tgaactagac 
     1981 aaaacagtaa ctgcagaaaa aagtcctata tgtgcaaaaa aatgcttgat gatttgtaat 
     2041 gagattgagg aggtttcgag acaggcacca aagtttttac aaatggatat ttccaccacc 
     2101 gctctaacca atttgttgaa ggaacaccct tggctatcct ttacaagatt cgaactgaag 
     2161 tggaaacagt tgtctcttat catttatgta ttaagagatt ttttcactaa ttttacccag 
     2221 aaaaagtcac aactagaaca ggatcaaaat gatcatcaaa gttatgaagt taaacgatgc 
     2281 tccatcatgt taagcgatgc agcacaaaga actgttatgt ctgtaagtag ctatatggac 
     2341 aatcataatg tcaccccata ttttgcctgg aattgttctt attacttgtt caatgcagtc 
     2401 ctagtaccca taaagactct actctcaaac tcaaaatcga atgctgagaa taacgagacc 
     2461 gcacaattat tacaacaaat taacactgtt ctgatgctat taaaaaaact ggccactttt 
     2521 aaaatccaga cttgtgaaaa atacattcaa gtactggaag aggtatgtgc gccgtttctg 
     2581 ttatcacagt gtgcaatccc attaccgcat atcagttata acaatagtaa tggtagcgcc 
     2641 attaaaaata tcgtcggttc tgcaactatc gcccaatacc ctactcttcc ggaggaaaat 
     2701 gtcaacaata tcagtgttaa atatgtttct cctggctcag tagggccttc acctgtgcca 
     2761 ttgaaatcag gagcaagttt cagtgatcta gtcaagctgt tatctaaccg tccaccctct 
     2821 cgtaactctc cagtgacaat accaagaagc acaccttcgc atcgctcagt cacgcctttt 
     2881 ctagggcaac agcaacagct gcaatcatta gtgccactga ccccgtctgc tttgtttggt 
     2941 ggcgccaatt ttaatcaaag tgggaatatc gctgatagct cattgtcctt cactttcact 
     3001 aacagtagca acggtccgaa cctcataaca actcaaacaa attctcaagc gctttcacaa 
     3061 ccaattgcct cctctaacgt tcatgataac ttcatgaata atgaaatcac ggctagtaaa 
     3121 attgatgatg gtaataattc aaaaccactg tcacctggtt ggacggacca aactgcgtat 
     3181 aacgcgtttg gaatcactac agggatgttt aataccacta caatggatga tgtatataac 
     3241 tatctattcg atgatgaaga taccccacca aacccaaaaa aagaggaggg ccgcggcagc 
     3301 ctgctgacct gcggcgatgt ggaggagaac cccgggcccg tgaagatcat ctcccgcaag 
     3361 tccctgggca cccagaacgt gtacgacatc ggcgtggaga aggaccacaa cttcctgctg 
     3421 aagaacggcc tggtggcctc caactgcttc aacggtaagt tattgaacaa tggcatcaaa 
     3481 tgccttcatc atcactaccc tttagccctt aagaccccac aatgacctta cccactcaga 
     3541 gaaaaaagta aatatgaaag cccatttgaa cttctgaagt tcctattctc tagaaagtat 
     3601 aggaacttct cctaggggat ctaattcaat tagagactaa ttcaattaga gctaattcaa 
     3661 ttaggatcca agcttatcga tttcgaaccc tcgaccgccg gagtataaat agaggcgctt 
     3721 cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct aagcgaaagc 
     3781 taagcaaata aacaagcgca gctgaacaag ctaaacaatc ggactagagc cggtcgccac 
     3841 catgaggtct tccaagaatg ttatcaagga gttcatgagg tttaaggttc gcatggaagg 
     3901 aacggtcaat gggcacgagt ttgaaataga aggcgaagga gaggggaggc catacgaagg 
     3961 ccacaatacc gtaaagctta aggtaaccaa ggggggacct ttgccatttg cttgggatat 
     4021 tttgtcacca caatttcagt atggaagcaa ggtatatgtc aagcaccctg ccgacatacc 
     4081 agactataaa aagctgtcat ttcctgaagg atttaaatgg gaaagggtca tgaactttga 
     4141 agacggtggc gtcgttactg taacccagga ttccagtttg caggatggct gtttcatcta 
     4201 caaggtcaag ttcattggcg tgaactttcc ttccgatgga cctgttatgc aaaagaagac 
     4261 aatgggctgg gaagccagca ctgagcgttt gtatcctcgt gatggcgtgt tgaaaggaga 
     4321 gattcataag gctctgaagc tgaaagacgg tggtcattac ctagttgagt tcaaaagtat 
     4381 ttacatggca aagaagcctg tgcagctacc agggtactac tatgttgact ccaaactgga 
     4441 tataacaagc cacaacgaag actatacaat cgttgagcag tatgaaagaa ccgagggacg 
     4501 ccaccatctg ttcctttagg atctttgtga aggaacctta cttctgtggt gtgacataat 
     4561 tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat 
     4621 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg 
     4681 atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa 
     4741 tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga 
     4801 agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg 
     4861 ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg 
     4921 cactgctata caagaaaatt atggaaaaat atttgatgta tagtgccttg actagagatc 
     4981 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc 
     5041 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct 
     5101 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 
     5161 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcgct 
     5221 agcagaagtt cctattctct agaaagtata ggaacttcaa ccccttgtgt catgtcggcg 
     5281 accctacgcc cccaactgag agaactcaaa ggttacccca gttggggcac tactcccgaa 
     5341 aaccgcttct gacctggggg cgcgccggta ccactagtgg ggaggttccc tttagtgagg 
     5401 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 
     5461 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 
     5521 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 
     5581 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 
     5641 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
     5701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 
     5761 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 
     5821 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 
     5881 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 
     5941 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 
     6001 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 
     6061 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 
     6121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 
     6181 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 
     6241 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 
     6301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 
     6361 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 
     6421 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 
     6481 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 
     6541 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 
     6601 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 
     6661 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 
     6721 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 
     6781 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 
     6841 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 
     6901 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 
     6961 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 
     7021 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 
     7081 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 
     7141 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 
     7201 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 
     7261 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 
     7321 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 
     7381 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 
     7441 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 
     7501 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 
     7561 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 
     7621 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 
     7681 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 
     7741 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 
     7801 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 
     7861 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 
     7921 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 
     7981 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttgcca 
     8041 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt 
     8101 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt 
     8161 ttcccagtca cgacg 
//
