LOCUS       psiT-GEM(2)        8175 bp    DNA     circular     24-APR-2024
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|-15416122|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     primer          27..43
                     /vntifkey="27"
                     /label=T7
     misc_feature    complement(6589..7449)
                     /vntifkey="21"
                     /label=Amp
     primer          3..18
                     /vntifkey="27"
                     /label=M13F
     misc_feature    72..171
                     /vntifkey="21"
                     /label=attP
     CDS             279..587
                     /vntifkey="4"
                     /label=EY-CfaN
     misc_feature    178..277
                     /vntifkey="21"
                     /label=SA
     misc_feature    588..641
                     /vntifkey="21"
                     /label=T2A
     CDS             642..3284
                     /vntifkey="4"
                     /label=Gal4
     misc_feature    3285..3338
                     /vntifkey="21"
                     /label=T2A
     CDS             3339..3452
                     /vntifkey="4"
                     /label=CfaC-CFN
     misc_feature    3455..3575
                     /vntifkey="21"
                     /label=SD
     misc_feature    3576..3609
                     /vntifkey="21"
                     /label=FRT
     misc_feature    5225..5258
                     /vntifkey="21"
                     /label=FRT
     misc_feature    3610..5224
                     /vntifkey="21"
                     /label=3XP3_RFP-SV40
     misc_feature    complement(5259..5358)
                     /vntifkey="21"
                     /label=attP
     misc_feature    52..71
                     /vntifkey="21"
                     /label=MCS
     misc_feature    5359..5378
                     /vntifkey="21"
                     /label=MCS
BASE COUNT     2276 a      1934 c      1834 g      2131 t 
ORIGIN
        1 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgacc caccggtgca 
       61 tgcgcggccg ccccaggtca gaagcggttt tcgggagtag tgccccaact ggggtaacct 
      121 ttgagttctc tcagttgggg gcgtagggtc gccgacatga cacaaggggt ttctagaagt 
      181 cgatccaaca tggcgacttg tcccatcccc ggcatgttta aatatactaa ttattcttga 
      241 actaatttta atcaaccgat ttatctctct tccgcaggga atattgcctg tcctacgaca 
      301 ccgagatcct gaccgtggag tacggcttcc tgcccatcgg caagatcgtg gaggagcgca 
      361 tcgagtgcac cgtgtacacc gtggacaaga acggcttcgt gtacacccag cccatcgccc 
      421 agtggcacaa ccgcggcgag caggaggtgt tcgagtactg cctggaggac ggctccatca 
      481 tccgcgccac caaggaccac aagttcatga ccaccgacgg ccagatgctg cccatcgacg 
      541 agatcttcga gcgcggcctg gacctgaagc aggtggacgg cctgcccgag ggccgcggca 
      601 gcctgctgac ctgcggcgat gtggaggaga accccgggcc catgaagcta ctgtcttcta 
      661 tcgaacaagc ctgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga 
      721 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt 
      781 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc 
      841 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac 
      901 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg 
      961 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa 
     1021 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat 
     1081 cgattgactc ggcagctcat catgataact ccacaattcc gttggatttt atgcccaggg 
     1141 atgctcttca tggatttgat tggtctgaag aggatgacat gtcggatggc ttgcccttcc 
     1201 tgaaaacgga ccccaacaat aatgggttct ttggcgacgg ttctctctta tgtattcttc 
     1261 gatctattgg ctttaaaccg gaaaattaca cgaactctaa cgttaacagg ctcccgacca 
     1321 tgattacgga tagatacacg ttggcttcta gatccacaac atcccgttta cttcaaagtt 
     1381 atctcaataa ttttcacccc tactgcccta tcgtgcactc accgacgcta atgatgttgt 
     1441 ataataacca gattgaaatc gcgtcgaagg atcaatggca aatccttttt aactgcatat 
     1501 tagccattgg agcctggtgt atagaggggg aatctactga tatagatgtt ttttactatc 
     1561 aaaatgctaa atctcatttg acgagcaagg tcttcgagtc aggttccata attttggtga 
     1621 cagccctaca tcttctgtcg cgatatacac agtggaggca gaaaacaaat actagctata 
     1681 attttcacag cttttccata agaatggcca tatcattggg cttgaatagg gacctcccct 
     1741 cgtccttcag tgatagcagc attctggaac aaagacgccg aatttggtgg tctgtctact 
     1801 cttgggagat ccaattgtcc ctgctttatg gtcgatccat ccagctttct cagaatacaa 
     1861 tctccttccc ttcttctgtc gacgatgtgc agcgtaccac aacaggtccc accatatatc 
     1921 atggcatcat tgaaacagca aggctcttac aagttttcac aaaaatctat gaactagaca 
     1981 aaacagtaac tgcagaaaaa agtcctatat gtgcaaaaaa atgcttgatg atttgtaatg 
     2041 agattgagga ggtttcgaga caggcaccaa agtttttaca aatggatatt tccaccaccg 
     2101 ctctaaccaa tttgttgaag gaacaccctt ggctatcctt tacaagattc gaactgaagt 
     2161 ggaaacagtt gtctcttatc atttatgtat taagagattt tttcactaat tttacccaga 
     2221 aaaagtcaca actagaacag gatcaaaatg atcatcaaag ttatgaagtt aaacgatgct 
     2281 ccatcatgtt aagcgatgca gcacaaagaa ctgttatgtc tgtaagtagc tatatggaca 
     2341 atcataatgt caccccatat tttgcctgga attgttctta ttacttgttc aatgcagtcc 
     2401 tagtacccat aaagactcta ctctcaaact caaaatcgaa tgctgagaat aacgagaccg 
     2461 cacaattatt acaacaaatt aacactgttc tgatgctatt aaaaaaactg gccactttta 
     2521 aaatccagac ttgtgaaaaa tacattcaag tactggaaga ggtatgtgcg ccgtttctgt 
     2581 tatcacagtg tgcaatccca ttaccgcata tcagttataa caatagtaat ggtagcgcca 
     2641 ttaaaaatat cgtcggttct gcaactatcg cccaataccc tactcttccg gaggaaaatg 
     2701 tcaacaatat cagtgttaaa tatgtttctc ctggctcagt agggccttca cctgtgccat 
     2761 tgaaatcagg agcaagtttc agtgatctag tcaagctgtt atctaaccgt ccaccctctc 
     2821 gtaactctcc agtgacaata ccaagaagca caccttcgca tcgctcagtc acgccttttc 
     2881 tagggcaaca gcaacagctg caatcattag tgccactgac cccgtctgct ttgtttggtg 
     2941 gcgccaattt taatcaaagt gggaatatcg ctgatagctc attgtccttc actttcacta 
     3001 acagtagcaa cggtccgaac ctcataacaa ctcaaacaaa ttctcaagcg ctttcacaac 
     3061 caattgcctc ctctaacgtt catgataact tcatgaataa tgaaatcacg gctagtaaaa 
     3121 ttgatgatgg taataattca aaaccactgt cacctggttg gacggaccaa actgcgtata 
     3181 acgcgtttgg aatcactaca gggatgttta ataccactac aatggatgat gtatataact 
     3241 atctattcga tgatgaagat accccaccaa acccaaaaaa agaggagggc cgcggcagcc 
     3301 tgctgacctg cggcgatgtg gaggagaacc ccgggcccgt gaagatcatc tcccgcaagt 
     3361 ccctgggcac ccagaacgtg tacgacatcg gcgtggagaa ggaccacaac ttcctgctga 
     3421 agaacggcct ggtggcctcc aactgcttca acaggtaagt tattgaacaa tggcatcaaa 
     3481 tgccttcatc atcactaccc tttagccctt aagaccccac aatgacctta cccactcaga 
     3541 gaaaaaagta aatatgaaag cccatttgaa cttctgaagt tcctattctc tagaaagtat 
     3601 aggaacttct cctaggggat ctaattcaat tagagactaa ttcaattaga gctaattcaa 
     3661 ttaggatcca agcttatcga tttcgaaccc tcgaccgccg gagtataaat agaggcgctt 
     3721 cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct aagcgaaagc 
     3781 taagcaaata aacaagcgca gctgaacaag ctaaacaatc ggactagagc cggtcgccac 
     3841 catgaggtct tccaagaatg ttatcaagga gttcatgagg tttaaggttc gcatggaagg 
     3901 aacggtcaat gggcacgagt ttgaaataga aggcgaagga gaggggaggc catacgaagg 
     3961 ccacaatacc gtaaagctta aggtaaccaa ggggggacct ttgccatttg cttgggatat 
     4021 tttgtcacca caatttcagt atggaagcaa ggtatatgtc aagcaccctg ccgacatacc 
     4081 agactataaa aagctgtcat ttcctgaagg atttaaatgg gaaagggtca tgaactttga 
     4141 agacggtggc gtcgttactg taacccagga ttccagtttg caggatggct gtttcatcta 
     4201 caaggtcaag ttcattggcg tgaactttcc ttccgatgga cctgttatgc aaaagaagac 
     4261 aatgggctgg gaagccagca ctgagcgttt gtatcctcgt gatggcgtgt tgaaaggaga 
     4321 gattcataag gctctgaagc tgaaagacgg tggtcattac ctagttgagt tcaaaagtat 
     4381 ttacatggca aagaagcctg tgcagctacc agggtactac tatgttgact ccaaactgga 
     4441 tataacaagc cacaacgaag actatacaat cgttgagcag tatgaaagaa ccgagggacg 
     4501 ccaccatctg ttcctttagg atctttgtga aggaacctta cttctgtggt gtgacataat 
     4561 tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat 
     4621 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg 
     4681 atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa 
     4741 tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga 
     4801 agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg 
     4861 ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg 
     4921 cactgctata caagaaaatt atggaaaaat atttgatgta tagtgccttg actagagatc 
     4981 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc 
     5041 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct 
     5101 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 
     5161 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcgct 
     5221 agcagaagtt cctattctct agaaagtata ggaacttcaa ccccttgtgt catgtcggcg 
     5281 accctacgcc cccaactgag agaactcaaa ggttacccca gttggggcac tactcccgaa 
     5341 aaccgcttct gacctggggg cgcgccggta ccactagtgg ggaggttccc tttagtgagg 
     5401 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 
     5461 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 
     5521 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 
     5581 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 
     5641 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
     5701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 
     5761 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 
     5821 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 
     5881 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 
     5941 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 
     6001 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 
     6061 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 
     6121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 
     6181 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 
     6241 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 
     6301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 
     6361 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 
     6421 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 
     6481 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 
     6541 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 
     6601 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 
     6661 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 
     6721 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 
     6781 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 
     6841 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 
     6901 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 
     6961 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 
     7021 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 
     7081 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 
     7141 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 
     7201 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 
     7261 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 
     7321 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 
     7381 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 
     7441 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 
     7501 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 
     7561 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 
     7621 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 
     7681 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 
     7741 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 
     7801 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 
     7861 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 
     7921 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 
     7981 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttgcca 
     8041 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt 
     8101 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt 
     8161 ttcccagtca cgacg 
//
