LOCUS       psiTE(0)-Gal4        6492 bp    DNA     circular     24-APR-2024
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
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COMMENT     VNTDBDATE|-15416128|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     primer          27..43
                     /vntifkey="27"
                     /label=T7
     CDS             complement(4906..5766)
                     /vntifkey="4"
                     /label=AmpC
     misc_feature    52..71
                     /vntifkey="21"
                     /label=MCS
     misc_feature    3676..3695
                     /vntifkey="21"
                     /label=MCS
     misc_feature    178..277
                     /vntifkey="21"
                     /label=SA
     misc_feature    278..283
                     /vntifkey="21"
                     /label=EY
     CDS             284..586
                     /vntifkey="4"
                     /label=CfaN
     CDS             587..640
                     /vntifkey="4"
                     /label=T2A
     CDS             3338..3451
                     /vntifkey="4"
                     /label=CfaC-CFN
     misc_marker     3452..3454
                     /vntifkey="22"
                     /label=Glu
     misc_feature    3455..3575
                     /vntifkey="21"
                     /label=SD
     misc_feature    72..171
                     /vntifkey="21"
                     /label=attB
     misc_feature    complement(3576..3675)
                     /vntifkey="21"
                     /label=attB
     CDS             641..3283
                     /vntifkey="4"
                     /label=Gal4
     CDS             3284..3337
                     /vntifkey="4"
                     /label=T2A
BASE COUNT     1732 a      1623 c      1477 g      1660 t 
ORIGIN
        1 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgacc caccggtgca 
       61 tgcgcggccg cgatgtaggt cacggtctcg aagccgcggt gcgggtgcca gggcgtgccc 
      121 ttgggctccc cgggcgcgta ctccacctca cccatctggt ccatcatgat gtctagaagt 
      181 cgatccaaca tggcgacttg tcccatcccc ggcatgttta aatatactaa ttattcttga 
      241 actaatttta atcaaccgat ttatctctct tccgcaggaa tattgcctgt cctacgacac 
      301 cgagatcctg accgtggagt acggcttcct gcccatcggc aagatcgtgg aggagcgcat 
      361 cgagtgcacc gtgtacaccg tggacaagaa cggcttcgtg tacacccagc ccatcgccca 
      421 gtggcacaac cgcggcgagc aggaggtgtt cgagtactgc ctggaggacg gctccatcat 
      481 ccgcgccacc aaggaccaca agttcatgac caccgacggc cagatgctgc ccatcgacga 
      541 gatcttcgag cgcggcctgg acctgaagca ggtggacggc ctgcccgagg gccgcggcag 
      601 cctgctgacc tgcggcgatg tggaggagaa ccccgggccc atgaagctac tgtcttctat 
      661 cgaacaagcc tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 
      721 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 
      781 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 
      841 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 
      901 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 
      961 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 
     1021 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 
     1081 gattgactcg gcagctcatc atgataactc cacaattccg ttggatttta tgcccaggga 
     1141 tgctcttcat ggatttgatt ggtctgaaga ggatgacatg tcggatggct tgcccttcct 
     1201 gaaaacggac cccaacaata atgggttctt tggcgacggt tctctcttat gtattcttcg 
     1261 atctattggc tttaaaccgg aaaattacac gaactctaac gttaacaggc tcccgaccat 
     1321 gattacggat agatacacgt tggcttctag atccacaaca tcccgtttac ttcaaagtta 
     1381 tctcaataat tttcacccct actgccctat cgtgcactca ccgacgctaa tgatgttgta 
     1441 taataaccag attgaaatcg cgtcgaagga tcaatggcaa atccttttta actgcatatt 
     1501 agccattgga gcctggtgta tagaggggga atctactgat atagatgttt tttactatca 
     1561 aaatgctaaa tctcatttga cgagcaaggt cttcgagtca ggttccataa ttttggtgac 
     1621 agccctacat cttctgtcgc gatatacaca gtggaggcag aaaacaaata ctagctataa 
     1681 ttttcacagc ttttccataa gaatggccat atcattgggc ttgaataggg acctcccctc 
     1741 gtccttcagt gatagcagca ttctggaaca aagacgccga atttggtggt ctgtctactc 
     1801 ttgggagatc caattgtccc tgctttatgg tcgatccatc cagctttctc agaatacaat 
     1861 ctccttccct tcttctgtcg acgatgtgca gcgtaccaca acaggtccca ccatatatca 
     1921 tggcatcatt gaaacagcaa ggctcttaca agttttcaca aaaatctatg aactagacaa 
     1981 aacagtaact gcagaaaaaa gtcctatatg tgcaaaaaaa tgcttgatga tttgtaatga 
     2041 gattgaggag gtttcgagac aggcaccaaa gtttttacaa atggatattt ccaccaccgc 
     2101 tctaaccaat ttgttgaagg aacacccttg gctatccttt acaagattcg aactgaagtg 
     2161 gaaacagttg tctcttatca tttatgtatt aagagatttt ttcactaatt ttacccagaa 
     2221 aaagtcacaa ctagaacagg atcaaaatga tcatcaaagt tatgaagtta aacgatgctc 
     2281 catcatgtta agcgatgcag cacaaagaac tgttatgtct gtaagtagct atatggacaa 
     2341 tcataatgtc accccatatt ttgcctggaa ttgttcttat tacttgttca atgcagtcct 
     2401 agtacccata aagactctac tctcaaactc aaaatcgaat gctgagaata acgagaccgc 
     2461 acaattatta caacaaatta acactgttct gatgctatta aaaaaactgg ccacttttaa 
     2521 aatccagact tgtgaaaaat acattcaagt actggaagag gtatgtgcgc cgtttctgtt 
     2581 atcacagtgt gcaatcccat taccgcatat cagttataac aatagtaatg gtagcgccat 
     2641 taaaaatatc gtcggttctg caactatcgc ccaataccct actcttccgg aggaaaatgt 
     2701 caacaatatc agtgttaaat atgtttctcc tggctcagta gggccttcac ctgtgccatt 
     2761 gaaatcagga gcaagtttca gtgatctagt caagctgtta tctaaccgtc caccctctcg 
     2821 taactctcca gtgacaatac caagaagcac accttcgcat cgctcagtca cgccttttct 
     2881 agggcaacag caacagctgc aatcattagt gccactgacc ccgtctgctt tgtttggtgg 
     2941 cgccaatttt aatcaaagtg ggaatatcgc tgatagctca ttgtccttca ctttcactaa 
     3001 cagtagcaac ggtccgaacc tcataacaac tcaaacaaat tctcaagcgc tttcacaacc 
     3061 aattgcctcc tctaacgttc atgataactt catgaataat gaaatcacgg ctagtaaaat 
     3121 tgatgatggt aataattcaa aaccactgtc acctggttgg acggaccaaa ctgcgtataa 
     3181 cgcgtttgga atcactacag ggatgtttaa taccactaca atggatgatg tatataacta 
     3241 tctattcgat gatgaagata ccccaccaaa cccaaaaaaa gaggagggcc gcggcagcct 
     3301 gctgacctgc ggcgatgtgg aggagaaccc cgggcccgtg aagatcatct cccgcaagtc 
     3361 cctgggcacc cagaacgtgt acgacatcgg cgtggagaag gaccacaact tcctgctgaa 
     3421 gaacggcctg gtggcctcca actgcttcaa cgaggtaagt tattgaacaa tggcatcaaa 
     3481 tgccttcatc atcactaccc tttagccctt aagaccccac aatgacctta cccactcaga 
     3541 gaaaaaagta aatatgaaag cccatttgaa cttctcatca tgatggacca gatgggtgag 
     3601 gtggagtacg cgcccgggga gcccaagggc acgccctggc acccgcaccg cggcttcgag 
     3661 accgtgacct acatcggcgc gccggtacca ctagtgggga ggttcccttt agtgagggtt 
     3721 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 
     3781 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 
     3841 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 
     3901 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 
     3961 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 
     4021 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 
     4081 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 
     4141 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 
     4201 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 
     4261 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 
     4321 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 
     4381 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 
     4441 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 
     4501 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 
     4561 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 
     4621 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 
     4681 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 
     4741 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 
     4801 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 
     4861 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 
     4921 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 
     4981 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 
     5041 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 
     5101 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 
     5161 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 
     5221 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 
     5281 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 
     5341 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 
     5401 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 
     5461 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 
     5521 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 
     5581 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 
     5641 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 
     5701 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 
     5761 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 
     5821 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 
     5881 ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 
     5941 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 
     6001 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 
     6061 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 
     6121 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 
     6181 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 
     6241 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 
     6301 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttgccattc 
     6361 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 
     6421 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 
     6481 ccagtcacga cg 
//
