LOCUS       psiTE(1)-Gal4        6486 bp    DNA     circular     24-APR-2024
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|-15416137|
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FEATURES             Location/Qualifiers
     primer          27..43
                     /vntifkey="27"
                     /label=T7
     misc_feature    complement(4900..5760)
                     /vntifkey="21"
                     /label=Amp
     primer          3..18
                     /vntifkey="27"
                     /label=M13F
     CDS             274..582
                     /vntifkey="4"
                     /label=EY-CfaN
     CDS             583..636
                     /vntifkey="4"
                     /label=T2A
     CDS             637..3279
                     /vntifkey="4"
                     /label=Gal4
     misc_feature    172..271
                     /vntifkey="21"
                     /label=SA
     misc_feature    72..171
                     /vntifkey="21"
                     /label=attB
     misc_feature    complement(3570..3669)
                     /vntifkey="21"
                     /label=attB
     CDS             3280..3333
                     /vntifkey="4"
                     /label=T2A
     CDS             3334..3447
                     /vntifkey="4"
                     /label=CfaC-CFN
     misc_feature    3449..3569
                     /vntifkey="21"
                     /label=SD
     primer          3739..3755
                     /vntifkey="27"
                     /label=M13\Reverse\Primer
BASE COUNT     1729 a      1622 c      1476 g      1659 t 
ORIGIN
        1 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgacc caccggtgca 
       61 tgcgcggccg cgatgtaggt cacggtctcg aagccgcggt gcgggtgcca gggcgtgccc 
      121 ttgggctccc cgggcgcgta ctccacctca cccatctggt ccatcatgat gagtcgatcc 
      181 aacatggcga cttgtcccat ccccggcatg tttaaatata ctaattattc ttgaactaat 
      241 tttaatcaac cgatttatct ctcttccgca ggtgaatatt gcctgtccta cgacaccgag 
      301 atcctgaccg tggagtacgg cttcctgccc atcggcaaga tcgtggagga gcgcatcgag 
      361 tgcaccgtgt acaccgtgga caagaacggc ttcgtgtaca cccagcccat cgcccagtgg 
      421 cacaaccgcg gcgagcagga ggtgttcgag tactgcctgg aggacggctc catcatccgc 
      481 gccaccaagg accacaagtt catgaccacc gacggccaga tgctgcccat cgacgagatc 
      541 ttcgagcgcg gcctggacct gaagcaggtg gacggcctgc ccgagggccg cggcagcctg 
      601 ctgacctgcg gcgatgtgga ggagaacccc gggcccatga agctactgtc ttctatcgaa 
      661 caagcctgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc 
      721 gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa aaggtctccg 
      781 ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga acagctattt 
      841 ctactgattt ttcctcgaga agaccttgac atgattttga aaatggattc tttacaggat 
      901 ataaaagcat tgttaacagg attatttgta caagataatg tgaataaaga tgccgtcaca 
      961 gatagattgg cttcagtgga gactgatatg cctctaacat tgagacagca tagaataagt 
     1021 gcgacatcat catcggaaga gagtagtaac aaaggtcaaa gacagttgac tgtatcgatt 
     1081 gactcggcag ctcatcatga taactccaca attccgttgg attttatgcc cagggatgct 
     1141 cttcatggat ttgattggtc tgaagaggat gacatgtcgg atggcttgcc cttcctgaaa 
     1201 acggacccca acaataatgg gttctttggc gacggttctc tcttatgtat tcttcgatct 
     1261 attggcttta aaccggaaaa ttacacgaac tctaacgtta acaggctccc gaccatgatt 
     1321 acggatagat acacgttggc ttctagatcc acaacatccc gtttacttca aagttatctc 
     1381 aataattttc acccctactg ccctatcgtg cactcaccga cgctaatgat gttgtataat 
     1441 aaccagattg aaatcgcgtc gaaggatcaa tggcaaatcc tttttaactg catattagcc 
     1501 attggagcct ggtgtataga gggggaatct actgatatag atgtttttta ctatcaaaat 
     1561 gctaaatctc atttgacgag caaggtcttc gagtcaggtt ccataatttt ggtgacagcc 
     1621 ctacatcttc tgtcgcgata tacacagtgg aggcagaaaa caaatactag ctataatttt 
     1681 cacagctttt ccataagaat ggccatatca ttgggcttga atagggacct cccctcgtcc 
     1741 ttcagtgata gcagcattct ggaacaaaga cgccgaattt ggtggtctgt ctactcttgg 
     1801 gagatccaat tgtccctgct ttatggtcga tccatccagc tttctcagaa tacaatctcc 
     1861 ttcccttctt ctgtcgacga tgtgcagcgt accacaacag gtcccaccat atatcatggc 
     1921 atcattgaaa cagcaaggct cttacaagtt ttcacaaaaa tctatgaact agacaaaaca 
     1981 gtaactgcag aaaaaagtcc tatatgtgca aaaaaatgct tgatgatttg taatgagatt 
     2041 gaggaggttt cgagacaggc accaaagttt ttacaaatgg atatttccac caccgctcta 
     2101 accaatttgt tgaaggaaca cccttggcta tcctttacaa gattcgaact gaagtggaaa 
     2161 cagttgtctc ttatcattta tgtattaaga gattttttca ctaattttac ccagaaaaag 
     2221 tcacaactag aacaggatca aaatgatcat caaagttatg aagttaaacg atgctccatc 
     2281 atgttaagcg atgcagcaca aagaactgtt atgtctgtaa gtagctatat ggacaatcat 
     2341 aatgtcaccc catattttgc ctggaattgt tcttattact tgttcaatgc agtcctagta 
     2401 cccataaaga ctctactctc aaactcaaaa tcgaatgctg agaataacga gaccgcacaa 
     2461 ttattacaac aaattaacac tgttctgatg ctattaaaaa aactggccac ttttaaaatc 
     2521 cagacttgtg aaaaatacat tcaagtactg gaagaggtat gtgcgccgtt tctgttatca 
     2581 cagtgtgcaa tcccattacc gcatatcagt tataacaata gtaatggtag cgccattaaa 
     2641 aatatcgtcg gttctgcaac tatcgcccaa taccctactc ttccggagga aaatgtcaac 
     2701 aatatcagtg ttaaatatgt ttctcctggc tcagtagggc cttcacctgt gccattgaaa 
     2761 tcaggagcaa gtttcagtga tctagtcaag ctgttatcta accgtccacc ctctcgtaac 
     2821 tctccagtga caataccaag aagcacacct tcgcatcgct cagtcacgcc ttttctaggg 
     2881 caacagcaac agctgcaatc attagtgcca ctgaccccgt ctgctttgtt tggtggcgcc 
     2941 aattttaatc aaagtgggaa tatcgctgat agctcattgt ccttcacttt cactaacagt 
     3001 agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc acaaccaatt 
     3061 gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag taaaattgat 
     3121 gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc gtataacgcg 
     3181 tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata taactatcta 
     3241 ttcgatgatg aagatacccc accaaaccca aaaaaagagg agggccgcgg cagcctgctg 
     3301 acctgcggcg atgtggagga gaaccccggg cccgtgaaga tcatctcccg caagtccctg 
     3361 ggcacccaga acgtgtacga catcggcgtg gagaaggacc acaacttcct gctgaagaac 
     3421 ggcctggtgg cctccaactg cttcaacggt aagttattga acaatggcat caaatgcctt 
     3481 catcatcact accctttagc ccttaagacc ccacaatgac cttacccact cagagaaaaa 
     3541 agtaaatatg aaagcccatt tgaacttctc atcatgatgg accagatggg tgaggtggag 
     3601 tacgcgcccg gggagcccaa gggcacgccc tggcacccgc accgcggctt cgagaccgtg 
     3661 acctacatcg gcgcgccggt accactagtg gggaggttcc ctttagtgag ggttaattgc 
     3721 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 
     3781 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 
     3841 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 
     3901 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 
     3961 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 
     4021 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 
     4081 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 
     4141 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
     4201 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
     4261 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
     4321 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
     4381 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
     4441 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
     4501 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
     4561 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 
     4621 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 
     4681 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
     4741 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
     4801 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
     4861 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 
     4921 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 
     4981 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 
     5041 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 
     5101 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 
     5161 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 
     5221 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 
     5281 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 
     5341 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 
     5401 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 
     5461 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 
     5521 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 
     5581 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 
     5641 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 
     5701 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 
     5761 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
     5821 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
     5881 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 
     5941 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 
     6001 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 
     6061 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 
     6121 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 
     6181 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 
     6241 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
     6301 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttgcc attcgccatt 
     6361 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 
     6421 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 
     6481 acgacg 
//
