LOCUS       psiTE(0)-p65AD        4905 bp    DNA     circular     24-APR-2024
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13432954|
COMMENT     VNTDBDATE|-15416150|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     primer          27..43
                     /vntifkey="27"
                     /label=T7
     CDS             complement(3319..4179)
                     /vntifkey="4"
                     /label=AmpC
     misc_feature    52..71
                     /vntifkey="21"
                     /label=MCS
     misc_feature    2089..2108
                     /vntifkey="21"
                     /label=MCS
     misc_feature    178..277
                     /vntifkey="21"
                     /label=SA
     misc_feature    278..283
                     /vntifkey="21"
                     /label=EY
     CDS             284..586
                     /vntifkey="4"
                     /label=CfaN
     CDS             587..640
                     /vntifkey="4"
                     /label=T2A
     CDS             1751..1864
                     /vntifkey="4"
                     /label=CfaC-CFN
     misc_marker     1865..1867
                     /vntifkey="22"
                     /label=Glu
     misc_feature    1868..1988
                     /vntifkey="21"
                     /label=SD
     misc_feature    72..171
                     /vntifkey="21"
                     /label=attB
     misc_feature    complement(1989..2088)
                     /vntifkey="21"
                     /label=attB
     CDS             1697..1750
                     /vntifkey="4"
                     /label=T2A
     CDS             641..1696
                     /vntifkey="4"
                     /label=p65AD
BASE COUNT     1126 a      1400 c      1274 g      1105 t 
ORIGIN
        1 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgacc caccggtgca 
       61 tgcgcggccg cgatgtaggt cacggtctcg aagccgcggt gcgggtgcca gggcgtgccc 
      121 ttgggctccc cgggcgcgta ctccacctca cccatctggt ccatcatgat gtctagaagt 
      181 cgatccaaca tggcgacttg tcccatcccc ggcatgttta aatatactaa ttattcttga 
      241 actaatttta atcaaccgat ttatctctct tccgcaggaa tattgcctgt cctacgacac 
      301 cgagatcctg accgtggagt acggcttcct gcccatcggc aagatcgtgg aggagcgcat 
      361 cgagtgcacc gtgtacaccg tggacaagaa cggcttcgtg tacacccagc ccatcgccca 
      421 gtggcacaac cgcggcgagc aggaggtgtt cgagtactgc ctggaggacg gctccatcat 
      481 ccgcgccacc aaggaccaca agttcatgac caccgacggc cagatgctgc ccatcgacga 
      541 gatcttcgag cgcggcctgg acctgaagca ggtggacggc ctgcccgagg gccgcggcag 
      601 cctgctgacc tgcggcgatg tggaggagaa ccccgggccc atggataaag cggaattaat 
      661 tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt tccacgccga tggagttcca 
      721 gtacctgccc gatacggatg accgtcaccg tatcgaagaa aagcggaagc gaacctatga 
      781 aaccttcaag tccatcatga aaaagtcccc cttctcgggc cccacggacc cgcgcccccc 
      841 gccccgtcgt attgcggttc cttcgcgcag cagtgccagc gtccccaaac ccgcaccgca 
      901 gccctacccg ttcacttcct cccttagcac gattaactat gatgagttcc ccacgatggt 
      961 gttccccagt ggacaaattt cccaggcatc ggcactggct ccggccccac cgcaagtcct 
     1021 cccccaggcg cccgctccgg caccggctcc cgcaatggtg agtgctctgg cccaggcccc 
     1081 cgctccagtc cccgtgctgg cgcctggacc cccacaggca gttgcccctc ctgctccgaa 
     1141 accaacgcag gcgggcgaag gaaccctgag cgaggccctc ttgcagcttc agttcgatga 
     1201 cgaagacttg ggagccctgc tgggtaacag cacagaccct gccgtattca ccgatctcgc 
     1261 atccgtggac aacagcgagt ttcagcagct cttgaatcag ggaatcccgg tcgcacctca 
     1321 taccacagag cccatgctga tggaataccc ggaggctatc acgcgactgg tgaccggcgc 
     1381 acagcgacca cccgatccag cccctgcccc actgggtgcc ccgggtttgc ccaatggcct 
     1441 cctcagcggc gatgaggatt tctccagcat cgctgatatg gatttctccg ctttgctgag 
     1501 ccagataagc tccgctagcg gaggaggtgg tggaggtgga ggtggaggta ctagtctgga 
     1561 gatcgaggcc gccttcctgg agcgcgagaa caccgccctg gagacccgcg tggccgagct 
     1621 gcgccagcgc gtgcagcgcc tgcgcaaccg cgtgagccag taccgcaccc gctacggccc 
     1681 cctgggcggc ggcaaggaag gacgcggatc gttgctgacg tgcggagacg tggaggagaa 
     1741 tccgggcccc gtgaagatca tctcccgcaa gtccctgggc acccagaacg tgtacgacat 
     1801 cggcgtggag aaggaccaca acttcctgct gaagaacggc ctggtggcct ccaactgctt 
     1861 caacgaggta agttattgaa caatggcatc aaatgccttc atcatcacta ccctttagcc 
     1921 cttaagaccc cacaatgacc ttacccactc agagaaaaaa gtaaatatga aagcccattt 
     1981 gaacttctca tcatgatgga ccagatgggt gaggtggagt acgcgcccgg ggagcccaag 
     2041 ggcacgccct ggcacccgca ccgcggcttc gagaccgtga cctacatcgg cgcgccggta 
     2101 ccactagtgg ggaggttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc 
     2161 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 
     2221 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 
     2281 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 
     2341 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 
     2401 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 
     2461 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 
     2521 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 
     2581 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 
     2641 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 
     2701 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 
     2761 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 
     2821 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 
     2881 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 
     2941 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 
     3001 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 
     3061 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 
     3121 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 
     3181 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 
     3241 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 
     3301 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 
     3361 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 
     3421 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 
     3481 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 
     3541 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 
     3601 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 
     3661 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 
     3721 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 
     3781 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 
     3841 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 
     3901 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 
     3961 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 
     4021 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 
     4081 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 
     4141 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 
     4201 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 
     4261 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg 
     4321 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 
     4381 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 
     4441 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 
     4501 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 
     4561 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 
     4621 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 
     4681 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt 
     4741 taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca actgttggga 
     4801 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 
     4861 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacg 
//
