LOCUS       psiTE(0)-LexAGAD        6699 bp    DNA     circular     26-APR-2024
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|-15419004|
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FEATURES             Location/Qualifiers
     primer          27..43
                     /vntifkey="27"
                     /label=T7
     CDS             complement(5113..5973)
                     /vntifkey="4"
                     /label=AmpC
     misc_feature    52..71
                     /vntifkey="21"
                     /label=MCS
     misc_feature    3883..3902
                     /vntifkey="21"
                     /label=MCS
     misc_feature    178..277
                     /vntifkey="21"
                     /label=SA
     misc_feature    278..283
                     /vntifkey="21"
                     /label=EY
     CDS             284..586
                     /vntifkey="4"
                     /label=CfaN
     CDS             587..640
                     /vntifkey="4"
                     /label=T2A
     CDS             3545..3658
                     /vntifkey="4"
                     /label=CfaC-CFN
     misc_marker     3659..3661
                     /vntifkey="22"
                     /label=Glu
     misc_feature    3662..3782
                     /vntifkey="21"
                     /label=SD
     misc_feature    72..171
                     /vntifkey="21"
                     /label=attB
     misc_feature    complement(3783..3882)
                     /vntifkey="21"
                     /label=attB
     CDS             3491..3544
                     /vntifkey="4"
                     /label=T2A
     CDS             641..3490
                     /vntifkey="4"
                     /label=LexAGAD
BASE COUNT     1645 a      1818 c      1687 g      1549 t 
ORIGIN
        1 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgacc caccggtgca 
       61 tgcgcggccg cgatgtaggt cacggtctcg aagccgcggt gcgggtgcca gggcgtgccc 
      121 ttgggctccc cgggcgcgta ctccacctca cccatctggt ccatcatgat gtctagaagt 
      181 cgatccaaca tggcgacttg tcccatcccc ggcatgttta aatatactaa ttattcttga 
      241 actaatttta atcaaccgat ttatctctct tccgcaggaa tattgcctgt cctacgacac 
      301 cgagatcctg accgtggagt acggcttcct gcccatcggc aagatcgtgg aggagcgcat 
      361 cgagtgcacc gtgtacaccg tggacaagaa cggcttcgtg tacacccagc ccatcgccca 
      421 gtggcacaac cgcggcgagc aggaggtgtt cgagtactgc ctggaggacg gctccatcat 
      481 ccgcgccacc aaggaccaca agttcatgac caccgacggc cagatgctgc ccatcgacga 
      541 gatcttcgag cgcggcctgg acctgaagca ggtggacggc ctgcccgagg gccgcggcag 
      601 cctgctgacc tgcggcgatg tggaggagaa ccccgggccc atgccaccca agaagaagcg 
      661 aaaagtagaa gatccaatga aggctctcac ggcccgacaa caggaagttt ttgatttgat 
      721 acgggatcat atatcccaaa cgggtatgcc tccgacccgc gcagagatag cacagcgact 
      781 gggctttcga tcgcctaacg ccgcggagga gcacttgaag gcactggccc gcaagggtgt 
      841 cattgaaatc gtgtccggtg cgagccgcgg aatccggctg ttgcaggagg aggaagaggg 
      901 cctgccactg gtgggacgcg tggccgctgg cgagccgctg ctggcccagc aacacataga 
      961 gggacactat caggtggacc cctccttgtt taagccaaat gctgatttcc tgttgcgggt 
     1021 gtcgggaatg tccatgaagg acatcggtat tatggatggt gacctcctcg ccgtccataa 
     1081 gacacaggat gtcaggaacg gccaggtagt cgttgccagg atagacgatg aggtcactgt 
     1141 gaaacgtctc aagaagcaag gcaataaggt cgagctgctg ccggagaata gcgagttcaa 
     1201 gccgatcgtg gtggatctgc gacagcagtc ctttactatc gagggcttgg ccgtgggtgt 
     1261 gatccgcaac ggagattggc tgggatccat cgacagtgcg gcccaccacg acaacagcac 
     1321 cattccactg gacttcatgc ctcgtgacgc actgcatgga tttgactgga gcgaggagga 
     1381 tgatatgagc gacggactcc cattcctgaa gaccgacccc aataacaatg gtttctttgg 
     1441 cgacggcagc ctgctgtgta tcctgcgaag tattggtttt aagccggaaa attacacgaa 
     1501 cagtaatgtc aatcgtcttc ccacaatgat caccgaccgt tacacgctgg ccagtcgcag 
     1561 tactacaagc cgcctgctgc aaagctatct taacaacttc cacccctact gccccatagt 
     1621 gcatagtcct actctcatga tgctttacaa caatcagatt gagatagcat cgaaggacca 
     1681 gtggcagatc ctcttcaact gcattctcgc catcggcgct tggtgtattg aaggcgagag 
     1741 cacagatatc gacgtgtttt actatcaaaa cgccaagtcg cacctcacct cgaaggtctt 
     1801 cgaaagtgga agcattatcc tcgtgacagc cctccatctg ctgagtcgtt atacccagtg 
     1861 gcgtcagaaa actaatacat cctacaactt tcattcgttc agcattcgga tggcgattag 
     1921 cctgggactt aatcgtgacc tcccaagtag cttcagcgat tcgtcgatcc tggaacagcg 
     1981 ccggaggatt tggtggagtg tgtacagctg ggagatccag ctgtccctct tgtacggacg 
     2041 cagcattcag ctgagtcaga acacgatttc cttcccgtcc agcgtggacg acgttcaacg 
     2101 gacaactacg ggcccgacca tctaccacgg tatcattgag acggcccgct tgcttcaagt 
     2161 gtttacgaag atttatgagc tggataagac agtgaccgcg gagaagagtc cgatctgcgc 
     2221 caagaaatgc ctgatgatct gcaatgagat agaggaggtg agccgccagg cacccaagtt 
     2281 cctgcagatg gacataagca ctacggcgct gactaatctc ctcaaagaac acccctggct 
     2341 gagtttcacc cgcttcgagc tgaagtggaa acagctgagc ctgattatat acgttttgcg 
     2401 ggactttttc acaaatttta cgcagaagaa gtcgcagctt gagcaagatc agaacgacca 
     2461 tcagtcctac gaggtaaaga ggtgcagcat aatgctctcc gacgcagccc agcgcaccgt 
     2521 gatgtcggtg tcgtcctata tggacaacca taatgtgacc ccttatttcg cctggaattg 
     2581 ctcctactat ttgttcaacg ccgtgttggt cccaatcaag actcttctga gcaactccaa 
     2641 atcgaacgcc gagaataacg agacagcaca gctcttgcag caaattaaca ccgtgttgat 
     2701 gctgttgaag aaactcgcta cattcaagat tcagacttgc gaaaagtaca tccaggtgtt 
     2761 ggaagaggtg tgcgccccat tccttctttc gcaatgcgca attcccctcc cgcacataag 
     2821 ttacaataat tccaacggct ccgctatcaa gaatatcgtc ggctccgcta caatcgcgca 
     2881 gtatcccacg ctgcctgagg aaaatgttaa caacatatcg gtgaagtatg taagtccagg 
     2941 cagcgtcggc ccgagccctg tgccgctgaa aagcggagcc tccttcagcg atctggtcaa 
     3001 gctgctcagc aaccgcccac catcccgcaa ctcccctgtc actatacccc gatcgacccc 
     3061 gtcgcatcgt agcgttaccc cattcctggg acaacagcag caactgcaga gtctggtgcc 
     3121 acttacgccc tcggcattgt ttggcggagc caacttcaac cagagtggta acattgcgga 
     3181 cagctccttg agcttcacgt ttaccaacag ctccaacggt ccaaacttga tcacgactca 
     3241 gacaaactcc caggcattgt cccagcccat tgccagctcg aatgtgcatg ataactttat 
     3301 gaataatgaa attactgcct ccaagattga cgacggcaac aacagcaagc ccctttcccc 
     3361 aggctggact gaccagacgg cctacaatgc tttcggtatc accactggca tgttcaacac 
     3421 cacaacgatg gacgacgtat ataactatct ttttgatgat gaggatactc caccgaaccc 
     3481 aaagaaggag gaaggacgcg gatcgttgct gacgtgcgga gacgtggagg agaatccggg 
     3541 ccccgtgaag atcatctccc gcaagtccct gggcacccag aacgtgtacg acatcggcgt 
     3601 ggagaaggac cacaacttcc tgctgaagaa cggcctggtg gcctccaact gcttcaacga 
     3661 ggtaagttat tgaacaatgg catcaaatgc cttcatcatc actacccttt agcccttaag 
     3721 accccacaat gaccttaccc actcagagaa aaaagtaaat atgaaagccc atttgaactt 
     3781 ctcatcatga tggaccagat gggtgaggtg gagtacgcgc ccggggagcc caagggcacg 
     3841 ccctggcacc cgcaccgcgg cttcgagacc gtgacctaca tcggcgcgcc ggtaccacta 
     3901 gtggggaggt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct 
     3961 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 
     4021 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 
     4081 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 
     4141 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 
     4201 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
     4261 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 
     4321 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 
     4381 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 
     4441 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 
     4501 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 
     4561 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 
     4621 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 
     4681 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 
     4741 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 
     4801 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 
     4861 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 
     4921 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 
     4981 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 
     5041 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 
     5101 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 
     5161 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 
     5221 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 
     5281 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 
     5341 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 
     5401 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 
     5461 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 
     5521 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 
     5581 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 
     5641 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 
     5701 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 
     5761 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 
     5821 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 
     5881 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 
     5941 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 
     6001 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 
     6061 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg 
     6121 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 
     6181 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 
     6241 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 
     6301 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 
     6361 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 
     6421 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 
     6481 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa 
     6541 aatattaacg cttacaattt gccattcgcc attcaggctg cgcaactgtt gggaagggcg 
     6601 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 
     6661 attaagttgg gtaacgccag ggttttccca gtcacgacg 
//
