LOCUS       pTrcHis_Vector_A_Short_ 3297 bp ds-DNA     circular     09-APR-2022
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name: pTrcHis Vector A
            Genomic speG.txt synthetic DNA NEBuilder-generated oligos
            (UPPERCASE = gene-specific, lowercase = overlap) Genomic speG_fwd:
            cctgatgcggtattttctccAAAATAGTAACGTTGTTTTAATCATCTTTGTC Genomic
            speG_fwd 3'Tm: 61.8  3'Ta: 62.8 Genomic speG_rev:
            cactaccatcggcgctacggCTATTGTGCGGTCGGCTTC Genomic speG_rev 3'Tm: 66.3
            3'Ta: 62.8 pTrcHis Vector A short_fwd: CCGTAGCGCCGATGGTAG pTrcHis
            Vector A short_fwd 3'Tm: 68.4  3'Ta: 67.1 pTrcHis Vector A
            short_rev: GGAGAAAATACCGCATCAGGC pTrcHis Vector A short_rev 3'Tm:
            66.1  3'Ta: 67.1
FEATURES             Location/Qualifiers
     rep_origin      227..909
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     977..997
                     /label="Seq Primer for Rev"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(1076..1096)
                     /label="2,900 R"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     1077..1128
                     /label="gene-specific primer: AAAATAGTAACGTTGTTTTAATCATCTTTGTC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     gene            1097..2192
                     /label="Genomic speG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     gene            1632..2192
                     /label="speG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             1632..2192
                     /label="Translation 1632-2192"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVELSDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAIRMCIFQHQYLAEHKTPGQTLLKPTAQ*"
     primer_bind     complement(2174..2212)
                     /label="gene-specific primer: CTATTGTGCGGTCGGCTTC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            2193..1096
                     /label="pTrcHis Vector A short"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     2193..2210
                     /label="700 F"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(2307..2327)
                     /label="Seq Primer for FWD end"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             2767..129
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
       61 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
      121 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
      181 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
      241 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
      301 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
      361 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
      421 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
      481 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
      541 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
      601 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
      661 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
      721 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      781 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      841 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      901 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      961 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     1021 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     1081 atgcggtatt ttctccaaaa tagtaacgtt gttttaatca tctttgtctc ttattgacat
     1141 catgtatagt tatagggcga cataatatca tcaatataaa caccctcctg gtacgttttg
     1201 cgtccgcagt ggatggtgta gaatcacctt taatcattca tacagggaat gaattatgaa
     1261 aatcactctc agcaaacgaa tcggcctgct cgctattctg ctgccttgcg cactggcatt
     1321 gagcacaact gttcatgccg aaactaacaa actggtgatt gagtctggcg acagtgcaca
     1381 aagccgccag cacgccgcta tggaaaaaga gcaatggaat gacacgcgca atctgcgcca
     1441 gaaagtgaat aaacgcactg aaaaagagtg ggataaagcc gacgccgctt ttgataaccg
     1501 cgataaatgt gagcaaagcg ccaacatcaa tgcctactgg gagcccaata ctttgcgctg
     1561 cctggaccgt cgaactggcc gcgttattac cccctaacct gttattgatt taaggaatgt
     1621 aaggacacgt tatgccaagc gcccacagtg ttaagctacg cccgctggag cgtgaagatt
     1681 tacgctatgt acatcaactc gacaataacg ccagtgtgat gcgttactgg tttgaggaac
     1741 cctacgaagc ctttgttgaa ctctctgatc tgtatgataa gcatattcac gatcagagcg
     1801 aacggcgctt tgtggtggaa tgtgacggcg aaaaagccgg tctggtggag ctggtggaaa
     1861 ttaaccatgt tcatcgccgc gcagaatttc agataattat ctccccggag tatcagggga
     1921 aaggtctggc aacccgtgcc gccaaattag caatggacta tggctttacc gttctcaatc
     1981 tctataagct gtatctgatc gttgataaag agaatgaaaa agcgattcac atttaccgca
     2041 agcttggctt ttcggttgaa ggtgaattga tgcacgagtt ctttattaat ggtcaatatc
     2101 gtaatgccat tcgcatgtgt atattccagc atcagtatct ggcagagcac aaaacaccgg
     2161 gtcagactct cctgaagccg accgcacaat agccgtagcg ccgatggtag tgtggggtct
     2221 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     2281 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     2341 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
     2401 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
     2461 gtttctacaa actctttttg tttatttttc taaatacatt caaatatgta tccgctcatg
     2521 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     2581 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     2641 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     2701 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2761 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     2821 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2881 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2941 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     3001 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     3061 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     3121 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     3181 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     3241 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatc
//