LOCUS       pTrcHis_Vector_A_Short_ 3246 bp ds-DNA     circular     20-JAN-2023
DEFINITION  synthetic DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name:
            pTrcHis_PA4114_speGprom.txt NEBuilder-generated oligos (UPPERCASE =
            gene-specific, lowercase = overlap) PA4114_fwd:
            aaggaatgtaaggacacgttATGATTGAACTGCGTACCATTACAC PA4114_fwd 3'Tm: 64.9
            3'Ta: 65.9 PA4114_rev: cactaccatcggcgctacggTCAGTGGTGGTGGTGGTG
            PA4114_rev 3'Tm: 67.6  3'Ta: 65.9 pTrcHis_speGpromoter_fwd:
            CCGTAGCGCCGATGGTAG pTrcHis_speGpromoter_fwd 3'Tm: 68.4  3'Ta: 65.6
            pTrcHis_speGpromoter_rev: AACGTGTCCTTACATTCCTTAAATCAATAAC
            pTrcHis_speGpromoter_rev 3'Tm: 64.6  3'Ta: 65.6
FEATURES             Location/Qualifiers
     rep_origin      224..906
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     974..994
                     /label="Seq Primer for Rev"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(1073..1093)
                     /label="2,900 R"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(1598..1628)
                     /label="pTrcHis_speGpromoter_rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="gene-specific Tm: 64.6 Ta: 65.6"
                     /note="gene-specific primer: AACGTGTCCTTACATTCCTTAAATCAATAAC"
     misc_feature    1609..1646
                     /label="PA4114_fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1629..2138
                     /label="PA4114"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             1629..2138
                     /label="PA4114"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MIELRTITRDDWETCIDLKVARHQAHFVASNLYSLAQSRFLPGFRAVGIHHGGRMVGFALYGPDADDGHYWLYRLMIDRRYQGKGHGRAALQRIIASVRRTRRRSDRLMVAYAVDNDVARQLYRDAGFVEQGLAPWGETMACLMLPEPSHRSGKVAGAPGGRAAHGVPA*"
     misc_feature    complement(2121..2158)
                     /label="PA4114_rev"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     gene            2139..1628
                     /label="pTrcHis_speGpromoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     2139..2156
                     /label="700 F"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     2139..2156
                     /label="pTrcHis_speGpromoter_fwd"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="gene-specific Tm: 68.4 Ta: 65.6"
                     /note="gene-specific primer: CCGTAGCGCCGATGGTAG"
     primer_bind     complement(2253..2273)
                     /label="Seq Primer for FWD end"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             2584..129
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             2713..126
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
       61 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
      121 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
      181 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
      241 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
      301 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
      361 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
      421 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
      481 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      541 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      601 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      661 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      721 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      781 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      841 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      901 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      961 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     1021 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     1081 cggtattttc tccaaaatag taacgttgtt ttaatcatct ttgtctctta ttgacatcat
     1141 gtatagttat agggcgacat aatatcatca atataaacac cctcctggta cgttttgcgt
     1201 ccgcagtgga tggtgtagaa tcacctttaa tcattcatac agggaatgaa ttatgaaaat
     1261 cactctcagc aaacgaatcg gcctgctcgc tattctgctg ccttgcgcac tggcattgag
     1321 cacaactgtt catgccgaaa ctaacaaact ggtgattgag tctggcgaca gtgcacaaag
     1381 ccgccagcac gccgctatgg aaaaagagca atggaatgac acgcgcaatc tgcgccagaa
     1441 agtgaataaa cgcactgaaa aagagtggga taaagccgac gccgcttttg ataaccgcga
     1501 taaatgtgag caaagcgcca acatcaatgc ctactgggag cccaatactt tgcgctgcct
     1561 ggaccgtcga actggccgcg ttattacccc ctaacctgtt attgatttaa ggaatgtaag
     1621 gacacgttat gattgaactg cgtaccatta cacgtgatga ttgggaaacc tgtattgatc
     1681 tgaaagttgc acgtcatcag gcacattttg ttgcaagcaa tctgtatagc ctggcacaga
     1741 gccgttttct gcctggtttt cgtgcagttg gtattcatca tggtggtcgt atggttggtt
     1801 ttgcactgta tggtccggat gcagatgatg gtcattattg gctgtatcgt ctgatgattg
     1861 atcgtcgcta tcaaggtaaa ggtcatggtc gtgcagcact gcagcgtatt attgcaagcg
     1921 ttcgtcgtac ccgtcgtcgt agcgatcgcc tgatggttgc atatgccgtt gataatgatg
     1981 ttgcccgtca gctgtaccgt gatgcaggtt ttgttgaaca aggtctggca ccgtggggtg
     2041 aaaccatggc atgtctgatg ctgccggaac cgagccatcg tagcggtaaa gttgccggtg
     2101 caccaggtgg tcgcgcagcc catggtgttc cggcataacc gtagcgccga tggtagtgtg
     2161 gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
     2221 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     2281 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg
     2341 acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct
     2401 ttttgcgttt ctacaaactc tttttgttta tttttctaaa tacattcaaa tatgtatccg
     2461 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     2521 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     2581 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2641 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2701 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     2761 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2821 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2881 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2941 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     3001 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     3061 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3121 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3181 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3241 atcatt
//