LOCUS       pTrcHis_Vector_A_short_ 3291 bp ds-DNA     circular     08-FEB-2023
DEFINITION  synthetic DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name:
            pTrcHis_speGprom_PA1472.txt NEBuilder-generated oligos (UPPERCASE =
            gene-specific, lowercase = overlap) PA1472_fwd:
            aaggaatgtaaggacacgttATGAACTTTGTTTTTCCGC PA1472_fwd 3'Tm: 58.3
            3'Ta: 59.3 PA1472_rev: cactaccatcggcgctacggTTAGCGATTACGCCAATG
            PA1472_rev 3'Tm: 59.5  3'Ta: 59.3 pTrcHis_speGprom_fwd:
            CCGTAGCGCCGATGGTAG pTrcHis_speGprom_fwd 3'Tm: 68.4  3'Ta: 65.6
            pTrcHis_speGprom_rev: AACGTGTCCTTACATTCCTTAAATCAATAAC
            pTrcHis_speGprom_rev 3'Tm: 64.6  3'Ta: 65.6
FEATURES             Location/Qualifiers
     primer_bind     complement(359..389)
                     /label="pTrcHis_speGprom_rev"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="gene-specific Tm: 64.6 Ta: 65.6"
                     /note="gene-specific primer: AACGTGTCCTTACATTCCTTAAATCAATAAC"
     primer_bind     370..408
                     /label="PA1472_fwd"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="gene-specific Tm: 58.3 Ta: 59.3"
                     /note="overlap: aaggaatgtaaggacacgtt"
                     /note="gene-specific primer: ATGAACTTTGTTTTTCCGC"
     gene            390..944
                     /label="PA1472"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             390..944
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MNFVFPHPLDTPRLRLRTFREEDAAPLFAMMSDPEVMRYWNTPPWTTPAQAREAILRDSQALVDGEYLNLAIERREDGQLLGSCILFHFEKGSRRAELGYCLARAAQGRGYMGEALRRLLAFAFDEIDLNRLEAEIDPRNRPSAASLERLGFRQEGLLAQRWIVSGEVSDSALYGLLAEHWRNR*"
     primer_bind     complement(927..964)
                     /label="PA1472_rev"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="gene-specific Tm: 59.5 Ta: 59.3"
                     /note="overlap: cactaccatcggcgctacgg"
                     /note="gene-specific primer: TTAGCGATTACGCCAATG"
     gene            945..389
                     /label="pTrcHis_speGprom"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     945..962
                     /label="pTrcHis_speGprom_fwd"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="gene-specific Tm: 68.4 Ta: 65.6"
                     /note="gene-specific primer: CCGTAGCGCCGATGGTAG"
     primer_bind     945..962
                     /label="700 F"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(1059..1079)
                     /label="Seq Primer for FWD end"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1390..2181
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1519..2178
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      2276..2958
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     3026..3046
                     /label="Seq Primer for Rev"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(3125..3145)
                     /label="2,900 R"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
ORIGIN
        1 cagggaatga attatgaaaa tcactctcag caaacgaatc ggcctgctcg ctattctgct
       61 gccttgcgca ctggcattga gcacaactgt tcatgccgaa actaacaaac tggtgattga
      121 gtctggcgac agtgcacaaa gccgccagca cgccgctatg gaaaaagagc aatggaatga
      181 cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa aaagagtggg ataaagccga
      241 cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc aacatcaatg cctactggga
      301 gcccaatact ttgcgctgcc tggaccgtcg aactggccgc gttattaccc cctaacctgt
      361 tattgattta aggaatgtaa ggacacgtta tgaactttgt ttttccgcat ccgctggata
      421 caccgcgtct gcgtctgcgc acctttcgtg aagaagatgc agcaccgctg tttgcaatga
      481 tgagcgatcc ggaagttatg cgttattgga atacccctcc gtggaccaca ccggcacagg
      541 cacgtgaagc aattctgcgt gatagccagg cactggttga tggtgaatat ctgaatctgg
      601 caattgaacg tcgtgaagat ggtcagctgc tgggtagctg tattctgttt cattttgaaa
      661 aaggtagccg tcgtgcagaa ctgggttatt gtctggcacg tgcagcacaa ggtcgtggtt
      721 atatgggtga agcactgcgt cgtctgctgg catttgcatt tgatgaaatt gatctgaatc
      781 gtctggaagc agaaatcgat ccgcgtaatc gtccgagcgc agcaagcctg gaacgtctgg
      841 gttttcgtca agaaggcctg ctggcacagc gttggattgt tagcggtgaa gttagcgata
      901 gcgcactgta tggactgctg gcagaacatt ggcgtaatcg ctaaccgtag cgccgatggt
      961 agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc
     1021 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
     1081 taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg
     1141 ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga
     1201 tggccttttt gcgtttctac aaactctttt tgtttatttt tctaaataca ttcaaatatg
     1261 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1321 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     1381 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1441 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1501 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1561 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1621 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1681 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1741 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     1801 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     1861 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1921 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1981 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     2041 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2101 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     2161 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     2221 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     2281 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2341 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2401 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2461 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     2521 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2581 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2641 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2701 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2761 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2821 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2881 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2941 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3001 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3061 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3121 gagcgcctga tgcggtattt tctccaaaat agtaacgttg ttttaatcat ctttgtctct
     3181 tattgacatc atgtatagtt atagggcgac ataatatcat caatataaac accctcctgg
     3241 tacgttttgc gtccgcagtg gatggtgtag aatcaccttt aatcattcat a
//