LOCUS       pTrcHis_Vector_A_Short_ 3252 bp ds-DNA     circular     13-MAY-2022
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name: New
            Assembly_assembledseq (3).gb synthetic DNA NEBuilder-generated
            oligos (UPPERCASE = gene-specific, lowercase = overlap) pTrcHis
            speG promoter_fwd: CCGTAGCGCCGATGGTAG pTrcHis speG promoter_fwd
            3'Tm: 68.4  3'Ta: 65.6 pTrcHis speG promoter_rev:
            AACGTGTCCTTACATTCCTTAAATCAATAAC pTrcHis speG promoter_rev 3'Tm:
            64.6  3'Ta: 65.6 SAT1_fwd:
            aaggaatgtaaggacacgttATGGCCAAATTTGTTATTCGTC SAT1_fwd 3'Tm: 61.1
            3'Ta: 62.1 SAT1_rev: cactaccatcggcgctacggCTATTCTTCGGTGGCCATTTTC
            SAT1_rev 3'Tm: 63.6  3'Ta: 62.1
FEATURES             Location/Qualifiers
     rep_origin      223..905
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     973..993
                     /label="Seq Primer for Rev"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(1072..1092)
                     /label="2,900 R"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(1597..1627)
                     /label="gene-specific primer: AACGTGTCCTTACATTCCTTAAATCAATAAC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     1608..1649
                     /label="gene-specific primer: ATGGCCAAATTTGTTATTCGTC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     gene            1628..2143
                     /label="SAT1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1628..2143
                     /label="Translation 1628-2143"
                     /translation="MAKFVIRPATAADCSDILRLIKELAKYEYMEEQVILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPEGHSIVGFAMYYFTYDPWIGKLLYLEDFFVMSDYRGFGIGSEILKNLSQVAMRCRCSSMHFLVAEWNEPSINFYKRRGASDLSSEEGWRLFKIDKEYLLKMATEE*"
     primer_bind     complement(2122..2163)
                     /label="gene-specific primer: CTATTCTTCGGTGGCCATTTTC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     gene            2144..1627
                     /label="pTrcHis speG promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     2144..2161
                     /label="gene-specific primer: CCGTAGCGCCGATGGTAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     2144..2161
                     /label="700 F"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(2258..2278)
                     /label="Seq Primer for FWD end"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             2718..125
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
       61 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
      121 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
      181 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
      241 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
      301 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
      361 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
      421 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
      481 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
      541 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
      601 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
      661 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      721 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      781 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      841 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      901 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      961 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     1021 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     1081 ggtattttct ccaaaatagt aacgttgttt taatcatctt tgtctcttat tgacatcatg
     1141 tatagttata gggcgacata atatcatcaa tataaacacc ctcctggtac gttttgcgtc
     1201 cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat tatgaaaatc
     1261 actctcagca aacgaatcgg cctgctcgct attctgctgc cttgcgcact ggcattgagc
     1321 acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag tgcacaaagc
     1381 cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct gcgccagaaa
     1441 gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga taaccgcgat
     1501 aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt gcgctgcctg
     1561 gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag gaatgtaagg
     1621 acacgttatg gccaaatttg ttattcgtcc ggcaaccgca gcagattgta gcgatattct
     1681 gcgtctgatt aaagaactgg ccaaatacga gtatatggaa gaacaggtta tcctgaccga
     1741 aaaagatctg ctggaagatg gttttggtga acatccgttt tatcattgtc tggttgcaga
     1801 agttccgaaa gaacattgga caccggaagg tcatagcatt gttggttttg ccatgtacta
     1861 tttcacctat gatccgtgga ttggcaaact gctgtatctg gaagatttct ttgtgatgag
     1921 cgattatcgc ggttttggta ttggttccga aattctgaaa aatctgagcc aggttgcaat
     1981 gcgttgtcgt tgtagcagca tgcattttct ggtggccgaa tggaatgaac cgagcattaa
     2041 tttctataaa cgtcgtggtg caagcgatct gagcagcgaa gaaggttggc gtctgtttaa
     2101 aatcgataaa gaatacctgc tgaaaatggc caccgaagaa tagccgtagc gccgatggta
     2161 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
     2221 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     2281 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
     2341 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
     2401 ggcctttttg cgtttctaca aactcttttt gtttattttt ctaaatacat tcaaatatgt
     2461 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     2521 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     2581 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     2641 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     2701 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     2761 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     2821 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     2881 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     2941 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3001 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3061 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     3121 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     3181 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     3241 gcggtatcat tg
//