LOCUS       pAB001_pAAV-Puro       11128 bp DNA     circular SYN 30-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Alessandro Bertero
  TITLE     Direct Submission
  JOURNAL   Exported Apr 30, 2024 from SnapGene 7.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11128
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    276..406
                     /label=SV40 pA
                     /label=sv40pA
     misc_feature    complement(413..1408)
                     /label=Trunc.TK
     promoter        complement(1421..2006)
                     /label=PGK-EM7 Promoter
     misc_feature    2026..2829
                     /label=AAVS1 HA-L
                     /note="'left homology arm to AAVS1"""
     misc_feature    2836..2861
                     /label=SA
                     /note="splice acceptor site"
     CDS             2885..2938
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2948..3547
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    3585..3719
                     /label=SV40 pA
                     /note="SV40 polyadenylation signal"
     promoter        join(3729..3927,3928..3946)
                     /label=H1 promoter
     enhancer        4037..4340
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4342..4619
                     /label=chicken beta-actin promoter
     intron          4621..5638
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             5706..6356
                     /codon_start=1
                     /label=OPT-tetR-nls
                     /translation="MPKKKRKVSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQ
                     PTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGA
                     KVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKE
                     ERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSPR"
     polyA_signal    6383..6569
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    6624..7460
                     /label=AAVS1 HA-R
                     /note="right homology arm of AAVS1"
     CDS             complement(9375..10235)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10359..10947
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggcagac atgataagat acattgatga
      301 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
      361 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttctta agtcagttag
      421 cctcccccat ctcccgggca aacgtgcgcg ccaggtcgca gatcgtcggt atggagcctg
      481 gggtggtgac gtgggtctgg atcatcccgg aggtaagttg cagcagggcg tcccggcagc
      541 cggcgggcga ttggtcgtaa tccaggataa agacgtgcat gggacggagg cgtttggcca
      601 agacgtccaa ggcccaggca aacacgttgt acaggtcgcc gttgggggcc agcaactcgg
      661 gggcccgaaa cagggtaaat aacgtgtccc cgatatgggg tcgtgggccc gcgttgctct
      721 ggggctcggc accctggggc ggcacggccg tccccgaaag ctgtccccaa tcctcccgcc
      781 acgacccgcc gccctgcaga taccgcaccg tattggcaag cagcccgtaa acgcggcgaa
      841 tcgcggccag catagccagg tcaagccgct cgccggggcg ctggcgtttg gccaggcggt
      901 cgatgtgtct gtcctccgga agggccccca acacgatgtt tgtgccgggc aaggtcggcg
      961 ggatgagggc cacgaacgcc agcacggcct ggggggtcat gctgcccata aggtatcgcg
     1021 cggccgggta gcacaggagg gcggcgatgg gatggcggtc gaagatgagg gtgagggccg
     1081 ggggcggggc atgtgagctc ccagcctccc ccccgatatg aggagccaga acggcgtcgg
     1141 tcacggcata aggcatgccc attgttatct gggcgcttgt cattaccacc gccgcgtccc
     1201 cggccgatat ctcaccctgg tcaaggcggt gttgtgtggt gtagatgttc gcgattgtct
     1261 cggaagcccc cagcacccgc cagtaagtca tcggctcggg tacgtagacg atatcgtcgc
     1321 gcgaacccag ggccaccagc agttgcgtgg tggtggtttt ccccatcccg tggggaccgt
     1381 ctatataaac ccgcagtagc gtgggcatgg tggccgatct ggtttagttc ctcaccttgt
     1441 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacaggc tgcaggtcga
     1501 aaggcccgga gatgaggaag aggagaacag cgcggcagac gtgcgctttt gaagcgtgca
     1561 gaatgccggg cctccggagg accttcgggc gcccgccccg cccctgagcc cgcccctgag
     1621 cccgcccccg gacccacccc ttcccagcct ctgagcccag aaagcgaagg agcaaagctg
     1681 ctattggccg ctgccccaaa ggcctacccg cttccattgc tcagcggtgc tgtccatctg
     1741 cacgagacta gtgagacgtg ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg
     1801 ggcggggggg aacttcctga ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa
     1861 gaacggagcc ggttggcgcc taccggtgga tgtggaatgt gtgcgaggcc agaggccact
     1921 tgtgtagcgc caagtgccca gcggggctgc taaagcgcat gctccagact gccttgggaa
     1981 aagcgcctcc cctacccggt agaattttta aacgaattcg ccctttgctt tctctgacca
     2041 gcattctctc ccctgggcct gtgccgcttt ctgtctgcag cttgtggcct gggtcacctc
     2101 tacggctggc ccagatcctt ccctgccgcc tccttcaggt tccgtcttcc tccactccct
     2161 cttccccttg ctctctgctg tgttgctgcc caaggatgct ctttccggag cacttccttc
     2221 tcggcgctgc accacgtgat gtcctctgag cggatcctcc ccgtgtctgg gtcctctccg
     2281 ggcatctctc ctccctcacc caaccccatg ccgtcttcac tcgctgggtt cccttttcct
     2341 tctccttctg gggcctgtgc catctctcgt ttcttaggat ggccttctcc gacggatgtc
     2401 tcccttgcgt cccgcctccc cttcttgtag gcctgcatca tcaccgtttt tctggacaac
     2461 cccaaagtac cccgtctccc tggctttagc cacctctcca tcctcttgct ttctttgcct
     2521 ggacaccccg ttctcctgtg gattcgggtc acctctcact cctttcattt gggcagctcc
     2581 cctacccccc ttacctctct agtctgtgct agctcttcca gccccctgtc atggcatctt
     2641 ccaggggtcc gagagctcag ctagtcttct tcctccaacc cgggccccta tgtccacttc
     2701 aggacagcat gtttgctgcc tccagggatc ctgtgtcccc gagctgggac caccttatat
     2761 tcccagggcc ggttaatgtg gctctggttc tgggtacttt tatctgtccc ctccacccca
     2821 cagtggggca agcttctgac ctcttctctt cctcccacag ggcctcgaga gttctggcag
     2881 cggagagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctag
     2941 gctcgagatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     3001 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     3061 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     3121 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     3181 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     3241 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     3301 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     3361 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     3421 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     3481 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     3541 tgcctgatct agagggcccg tttaaacccg ctgatcagcc tcgaaacttg tttattgcag
     3601 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3661 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcg
     3721 tcaattcgaa cgctgacgtc atcaacccgc tccaaggaat cgcgggccca gtgtcactag
     3781 gcgggaacac ccagcgcgcg tgcgccctgg caggaagatg gctgtgaggg acaggggagt
     3841 ggcgccctgc aatatttgca tgtcgctatg tgttctggga aatcaccata aacgtgaaat
     3901 gtctttggat ttgggaatct tataagttcc ctatcagtga tagagatcta agcttatcga
     3961 taccgtcgac ctcgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     4021 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     4081 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     4141 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     4201 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     4261 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     4321 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
     4381 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
     4441 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
     4501 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
     4561 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
     4621 ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
     4681 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
     4741 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
     4801 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
     4861 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
     4921 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     4981 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     5041 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     5101 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     5161 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     5221 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     5281 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     5341 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     5401 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     5461 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     5521 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     5581 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     5641 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat taattcggat
     5701 ccaccatgcc caagaaaaag cggaaggtgt cccggctgga caagagcaaa gtgatcaaca
     5761 gcgccctgga actgctgaac gaagtgggca tcgagggcct gaccacccgg aagctggccc
     5821 agaaactggg cgtggaacag cccaccctgt actggcacgt gaagaacaag cgggccctgc
     5881 tggacgccct ggccatcgag atgctggacc ggcaccacac acacttttgc cccctggaag
     5941 gcgaaagctg gcaggacttc ctgcggaaca acgccaagag cttcagatgc gccctgctga
     6001 gccaccggga cggcgccaaa gtgcacctgg gcaccagacc caccgagaag cagtacgaga
     6061 cactggaaaa ccagctggcc ttcctgtgcc agcagggctt cagcctggaa aacgccctgt
     6121 acgccctgag cgccgtgggc cactttaccc tgggctgcgt gctggaagat caggaacacc
     6181 aggtcgccaa agaggaaaga gagacaccca ccaccgacag catgcccccc ctgctgagac
     6241 aggccatcga gctgttcgat catcaaggcg ccgagcccgc cttcctgttc ggcctggaac
     6301 tgatcatctg cggcctcgag aagcagctga agtgcgagag cggctccccc agatgaacgc
     6361 gtagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     6421 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     6481 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     6541 caggacagca agggggagga ttgggaagag aatagcaggc atgctggggg tctcgagggg
     6601 gggcccggac agtactaagc tttactaggg acaggattgg tgacagaaaa gccccatcct
     6661 taggcctcct ccttcctagt ctcctgatat tgggtctaac ccccacctcc tgttaggcag
     6721 attccttatc tggtgacaca cccccatttc ctggagccat ctctctcctt gccagaacct
     6781 ctaaggtttg cttacgatgg agccagagag gatcctggga gggagagctt ggcagggggt
     6841 gggagggaag ggggggatgc gtgacctgcc cggttctcag tggccaccct gcgctaccct
     6901 ctcccagaac ctgagctgct ctgacgcggc tgtctggtgc gtttcactga tcctggtgct
     6961 gcagcttcct tacacttccc aagaggagaa gcagtttgga aaaacaaaat cagaataagt
     7021 tggtcctgag ttctaacttt ggctcttcac ctttctagtc cccaatttat attgttcctc
     7081 cgtgcgtcag ttttacctgt gagataaggc cagtagccag ccccgtcctg gcagggctgt
     7141 ggtgaggagg ggggtgtccg tgtggaaaac tccctttgtg agaatggtgc gtcctaggtg
     7201 ttcaccaggt cgtggccgcc tctactccct ttctctttct ccatccttct ttccttaaag
     7261 agtccccagt gctatctggg acatattcct ccgcccagag cagggtcccg cttccctaag
     7321 gccctgctct gggcttctgg gtttgagtcc ttggcaagcc caggagaggc gctcaggctt
     7381 ccctgtcccc cttcctcgtc caccatctca tgcccctggc tctcctgccc cttccctaca
     7441 ggggttcctg gctctgctct aagggcaagg gcgaattcgc ggccgctaaa ttcaattcgc
     7501 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     7561 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     7621 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagccta tacgtacggc
     7681 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     7741 gtgatattat tgacacgccg gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     7801 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     7861 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     7921 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     7981 aaatgtcagg catgagatta tcaaaaagga tcttcaccta gatccttttc acgtagaaag
     8041 ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa
     8101 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc
     8161 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg
     8221 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat
     8281 ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     8341 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     8401 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     8461 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     8521 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     8581 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     8641 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     8701 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     8761 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     8821 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
     8881 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     8941 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     9001 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     9061 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     9121 tctgaattat taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg
     9181 gtatttcaca ccgcatcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     9241 ttatttttct aaatacattc aaatatgtat ccgctcatga gattatcaaa aaggatcttc
     9301 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9361 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9421 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9481 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9541 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9601 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9661 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9721 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9781 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9841 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9901 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9961 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10021 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10081 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10141 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10201 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10261 agcatttatc agggttattg tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    10321 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    10381 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    10441 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    10501 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    10561 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    10621 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    10681 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    10741 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    10801 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    10861 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    10921 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    10981 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    11041 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
    11101 agcgagtcag tgagcgagga agcggaag
//
