LOCUS       Exported               10354 bp ds-DNA     circular SYN 28-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10354)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 1, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10354
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        144..384
                     /label=U6 Promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     315..334
                     /label=LKO1-5
     misc_RNA        411..486
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the CRISPR/Cas9 system"
     promoter        573..1751
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     1699..1719
                     /label=EF1a-F
     primer_bind     1768..1787
                     /label=T7
     promoter        1768..1786
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1800..1856
                     /codon_start=1
                     /label=bpNLS
                     /translation="MKRTADGSEFESPKKKRKV"
     CDS             1857..2531
                     /codon_start=1
                     /label=hUNG2
                     /translation="NVPVGFGESWKKHLSGEFGKPYFIKLMGFVAEERKHYTVYPPPHQ
                     VFTWTQMCDIKDVKVVILGQDPAHGPNQAHGLCFSVQRPVPPPPSLENIYKELSTDIED
                     FVHPGHGDLSGWAKQGVLLLNTVLTVRAHQANSHKERGWEQFTDAVVSWLNQNSNGLVF
                     LLWGSYAAKKGSAIDRKRHHVLQTAHPSPLSVDRGFFGCRHFSKTNELLQKSGKKPIDW
                     KEL"
     misc_feature    2058..2060
                     /label=Y156A
     misc_feature    2232..2234
                     /label=A214T
     variation       2367..2369
                     /label=Q259A
     variation       2442..2444
                     /label=Y284D
     CDS             2532..2627
                     /codon_start=1
                     /label=Linker-32aa
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             2628..6728
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          4761
                     /label=IgHV-Intron
                     /note="  The intron introduced in
                     SpCas9 (see Supplementary Fig. 11) derives from the mouse
                     immunoglobulin heavy chain precursor V-region intron
                     (GenBank ID: M12880.1), previously used
                     with different flanking exons.  AH002574.2
                     Petris, G., et al. (2017). ""Hit and go CAS9 delivered 
                     through a lentiviral based self-limiting circuit."" Nat 
                     Commun, IF=11.878. 8: 15334.
                     "
     CDS             6729..6740
                     /codon_start=1
                     /label=linker
                     /translation="SGGS"
     CDS             6741..6791
                     /codon_start=1
                     /label=bpNLS
                     /translation="KRTADGSEFEPKKKRKV"
     polyA_signal    6803..6937
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     6816..6836
                     /label=EBV-Reverse
     primer_bind     complement(6840..6859)
                     /label=SV40pA-R
     primer_bind     6879..6898
                     /label=PIRES-R
     primer_bind     6902..6921
                     /label=PCMV-R
     misc_feature    6951..7749
                     /label=CBH promoter
     promoter        7238..7515
                     /label=chicken beta-actin promoter
     CDS             7770..8480
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    8503..8727
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(8735..9323)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9494..10354)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct gacgtctaag taggagggcc tatttcccat gattccttca tatttgcata
      181 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
      241 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      301 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      361 ctttatatat cttgtggaaa ggacgaaaca ccgggtcttc gagaagacct gttttagagc
      421 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
      481 cggtgctttt ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttttta
      541 gcgcgtgcgc caattctgca gacaaatggc tcggctccgg tgcccgtcag tgggcagagc
      601 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
      661 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
      721 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
      781 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
      841 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
      901 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
      961 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa
     1021 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     1081 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     1141 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     1201 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     1261 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     1321 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     1381 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     1441 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     1501 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     1561 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga
     1621 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     1681 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc
     1741 aggtgtcgtg aggaattagc ttggtactaa tacgactcac tatagggaga gccgccacca
     1801 tgaaacggac agccgacgga agcgagttcg agtcaccaaa gaagaagcgg aaagtcaacg
     1861 tgcccgtggg ctttggagag agctggaaga agcacctcag cggggagttc gggaaaccgt
     1921 attttatcaa gctaatggga tttgttgcag aagaaagaaa gcattacact gtttatccac
     1981 ccccacacca agtgttcacc tggacccaga tgtgtgacat aaaagatgtg aaggttgtca
     2041 tcctgggaca ggatccagcc catggaccta atcaagctca cgggctctgc tttagtgttc
     2101 aaaggcctgt tccgcctccg cccagtttgg agaacattta taaagagttg tctacagaca
     2161 tagaggattt tgttcatcct ggccatggag atttatctgg gtgggccaag caaggtgttc
     2221 tccttctcaa caccgtcctc acggttcgtg cccatcaagc caactctcat aaggagcgag
     2281 gctgggagca gttcactgat gcagttgtgt cctggctaaa tcagaactcg aatggccttg
     2341 ttttcttgct ctggggctct tatgctgcca agaagggcag tgccattgat aggaagcggc
     2401 accatgtact acagacggct catccctccc ctttgtcagt ggacagaggg ttctttggat
     2461 gtagacactt ttcaaagacc aatgagctgc tgcagaagtc tggcaagaag cccattgact
     2521 ggaaggagct gtccggagga tctagcggag gctcctctgg ctctgagaca cctggcacaa
     2581 gcgagagcgc aacacctgaa agcagcgggg gcagcagcgg ggggtcagac aagaagtaca
     2641 gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     2701 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     2761 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
     2821 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     2881 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     2941 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     3001 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     3061 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     3121 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     3181 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     3241 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     3301 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     3361 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     3421 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     3481 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     3541 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     3601 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     3661 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     3721 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     3781 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     3841 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     3901 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     3961 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     4021 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     4081 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     4141 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     4201 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     4261 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     4321 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     4381 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     4441 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     4501 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     4561 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     4621 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     4681 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     4741 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     4801 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     4861 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     4921 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     4981 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     5041 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     5101 aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct
     5161 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     5221 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     5281 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     5341 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     5401 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     5461 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     5521 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     5581 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     5641 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     5701 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     5761 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     5821 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     5881 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     5941 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     6001 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     6061 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     6121 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     6181 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     6241 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     6301 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     6361 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     6421 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     6481 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     6541 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     6601 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     6661 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     6721 gaggtgactc tggcggctca aaaagaaccg ccgacggcag cgaattcgag cccaagaaga
     6781 agaggaaagt ctaaacgcgt cgaacttgtt tattgcagct tataatggtt acaaataaag
     6841 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6901 gtccaaactc atcaatgtat cttatcatgt ctggatcggc ggccggtacc cgttacataa
     6961 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     7021 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     7081 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     7141 ggtaaatggc ccgcctggca ttgtgcccag tacatgacct tatgggactt tcctacttgg
     7201 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
     7261 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
     7321 ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccagg cggggcgggg
     7381 cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc
     7441 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
     7501 agcgcgcggc gggcgggagt cgctgcgacg ctgccttcgc cccgtgcccc gctccgccgc
     7561 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     7621 ggacggccct tctcctccgg gctgtaatta gctgagcaag aggtaagggt ttaagggatg
     7681 gttggttggt ggggtattaa tgtttaatta cctggagcac ctgcctgaaa tcactttttt
     7741 tcaggttggc agatccgcta gccgccacca tggtgagcaa gggcgaggag gataacatgg
     7801 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     7861 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     7921 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     7981 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     8041 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgatgacggc ggcgtggtga
     8101 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     8161 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa aaccatgggc tgggaggcct
     8221 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     8281 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     8341 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     8401 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     8461 tggacgagct gtacaagtaa accggtctct tggcccggat ccctgtgcct tctagttgcc
     8521 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     8581 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     8641 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaaaac aatagcaggc
     8701 atgctgggga tgcggtgggc tctatggtgg aagctttcca taggctccgc ccccctgacg
     8761 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8821 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8881 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8941 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9001 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9061 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9121 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     9181 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9241 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9301 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9361 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9421 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9481 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9541 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9601 taccatctgg ccccagtgct gcaatgatac cgcggcttcc acgctcaccg gctccagatt
     9661 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9721 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9781 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9841 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9901 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9961 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10021 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10081 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10141 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10201 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10261 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10321 gaataagggc gacacggaaa tgttgaatac tcat
//
