LOCUS       Exported               10462 bp ds-DNA     circular SYN 28-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 1, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10462
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        144..384
                     /label=U6 Promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     315..334
                     /label=LKO1-5
     misc_RNA        411..486
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the CRISPR/Cas9 system"
     promoter        573..1751
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     1699..1719
                     /label=EF1a-F
     primer_bind     1768..1787
                     /label=T7
     promoter        1768..1786
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1800..1856
                     /codon_start=1
                     /label=bpNLS
                     /translation="MKRTADGSEFESPKKKRKV"
     CDS             1857..1868
                     /codon_start=1
                     /label=linker
                     /translation="SGGS"
     CDS             1869..5609
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)-N
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGS"
     CDS             5610..5705
                     /codon_start=1
                     /label=Linker1
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             5706..6380
                     /codon_start=1
                     /label=hUNG2
                     /translation="NVPVGFGESWKKHLSGEFGKPYFIKLMGFVAEERKHYTVYPPPHQ
                     VFTWTQMCDIKDVKVVILGQDPAHGPNQAHGLCFSVQRPVPPPPSLENIYKELSTDIED
                     FVHPGHGDLSGWAKQGVLLLNTVLTVRAHQANSHKERGWEQFTDAVVSWLNQNSNGLVF
                     LLWGSYAAKKGSAIDRKRHHVLQTAHPSPLSVDRGFFGCRHFSKTNELLQKSGKKPIDW
                     KEL"
     misc_feature    5907..5909
                     /label=Y156A
     misc_feature    6081..6083
                     /label=A214T
     variation       6216..6218
                     /label=Q259A
     variation       6291..6293
                     /label=Y284D
     CDS             6381..6476
                     /codon_start=1
                     /label=Linker2
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             6477..6836
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="PEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSA
                     YNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSI
                     TGLYETRIDLSQLGGD"
     CDS             6849..6899
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=bpNLS
                     /translation="KRTADGSEFEPKKKRKV"
     polyA_signal    6911..7045
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     6924..6944
                     /label=EBV-Reverse
     primer_bind     complement(6948..6967)
                     /label=SV40pA-R
     primer_bind     6987..7006
                     /label=PIRES-R
     primer_bind     7010..7029
                     /label=PCMV-R
     misc_feature    7059..7857
                     /label=CBH promoter
     promoter        7346..7623
                     /label=chicken beta-actin promoter
     CDS             7878..8588
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    8611..8835
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(8843..9431)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9602..10462)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct gacgtctaag taggagggcc tatttcccat gattccttca tatttgcata
      181 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
      241 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      301 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      361 ctttatatat cttgtggaaa ggacgaaaca ccgggtcttc gagaagacct gttttagagc
      421 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
      481 cggtgctttt ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttttta
      541 gcgcgtgcgc caattctgca gacaaatggc tcggctccgg tgcccgtcag tgggcagagc
      601 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
      661 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
      721 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
      781 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
      841 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
      901 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
      961 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa
     1021 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     1081 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     1141 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     1201 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     1261 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     1321 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     1381 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     1441 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     1501 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     1561 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga
     1621 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     1681 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc
     1741 aggtgtcgtg aggaattagc ttggtactaa tacgactcac tatagggaga gccgccacca
     1801 tgaaacggac agccgacgga agcgagttcg agtcaccaaa gaagaagcgg aaagtctctg
     1861 gcggctcaga caagaagtac agcatcggcc tggccatcgg caccaactct gtgggctggg
     1921 ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg
     1981 accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag
     2041 ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga
     2101 tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc
     2161 acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct
     2221 tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga
     2281 gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg
     2341 cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca
     2401 gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa
     2461 accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga
     2521 gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg
     2581 gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg
     2641 ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc
     2701 tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg
     2761 ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg
     2821 cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg
     2881 tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct
     2941 acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca
     3001 tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc
     3061 tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc
     3121 tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa
     3181 agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa
     3241 acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg
     3301 aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg
     3361 ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca
     3421 ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct
     3481 tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag
     3541 tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg
     3601 aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga
     3661 aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata
     3721 tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct
     3781 atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct
     3841 ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa
     3901 tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg
     3961 acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata
     4021 gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc
     4081 agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca
     4141 tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg
     4201 agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac
     4261 accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg
     4321 ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg
     4381 accatatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca
     4441 gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga
     4501 tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca
     4561 atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga
     4621 gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga
     4681 tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga
     4741 agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca
     4801 acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca
     4861 aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc
     4921 ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct
     4981 acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga
     5041 agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg
     5101 attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg
     5161 aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc
     5221 tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg
     5281 tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga
     5341 gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca
     5401 tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc
     5461 ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg
     5521 aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg
     5581 ccagccacta tgagaagctg aagggctcca gtggaggctc atcaggaggt tcatcaggct
     5641 cagagacacc cggaacctct gaaagcgcca caccagagag ctctggtgga tccagtgggg
     5701 gttcaaacgt gcccgtgggc tttggagaga gctggaagaa gcacctcagc ggggagttcg
     5761 ggaaaccgta ttttatcaag ctaatgggat ttgttgcaga agaaagaaag cattacactg
     5821 tttatccacc cccacaccaa gtgttcacct ggacccagat gtgtgacata aaagatgtga
     5881 aggttgtcat cctgggacag gatccagccc atggacctaa tcaagctcac gggctctgct
     5941 ttagtgttca aaggcctgtt ccgcctccgc ccagtttgga gaacatttat aaagagttgt
     6001 ctacagacat agaggatttt gttcatcctg gccatggaga tttatctggg tgggccaagc
     6061 aaggtgttct ccttctcaac accgtcctca cggttcgtgc ccatcaagcc aactctcata
     6121 aggagcgagg ctgggagcag ttcactgatg cagttgtgtc ctggctaaat cagaactcga
     6181 atggccttgt tttcttgctc tggggctctt atgctgccaa gaagggcagt gccattgata
     6241 ggaagcggca ccatgtacta cagacggctc atccctcccc tttgtcagtg gacagagggt
     6301 tctttggatg tagacacttt tcaaagacca atgagctgct gcagaagtct ggcaagaagc
     6361 ccattgactg gaaggagctg tccgggggta gctctggcgg cagcagtgga tcagagacac
     6421 ccggtacctc cgagagcgca accccagagt cctctggtgg gtctagcggt ggcagtcccg
     6481 aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca
     6541 tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag
     6601 tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca
     6661 tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca
     6721 ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc
     6781 agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga ggtgactctg
     6841 gcggctcaaa aagaaccgcc gacggcagcg aattcgagcc caagaagaag aggaaagtct
     6901 aaacgcgtcg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     6961 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7021 caatgtatct tatcatgtct ggatcggcgg ccggtacccg ttacataact tacggtaaat
     7081 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaatagt aacgccaata
     7141 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     7201 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     7261 gcctggcatt gtgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     7321 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     7381 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     7441 gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg
     7501 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     7561 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     7621 gcgggagtcg ctgcgacgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
     7681 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     7741 tcctccgggc tgtaattagc tgagcaagag gtaagggttt aagggatggt tggttggtgg
     7801 ggtattaatg tttaattacc tggagcacct gcctgaaatc actttttttc aggttggcag
     7861 atccgctagc cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     7921 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     7981 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     8041 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     8101 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     8161 gcttcaagtg ggagcgcgtg atgaacttcg atgacggcgg cgtggtgacc gtgacccagg
     8221 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     8281 cctccgacgg ccccgtaatg cagaagaaaa ccatgggctg ggaggcctcc tccgagcgga
     8341 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     8401 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     8461 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     8521 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     8581 acaagtaaac cggtctcttg gcccggatcc ctgtgccttc tagttgccag ccatctgttg
     8641 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     8701 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     8761 gggtggggca ggacagcaag ggggaggatt gggaaaacaa tagcaggcat gctggggatg
     8821 cggtgggctc tatggtggaa gctttccata ggctccgccc ccctgacgag catcacaaaa
     8881 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8941 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9001 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9061 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9121 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9181 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9241 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     9301 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9361 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9421 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9481 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9541 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9601 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9661 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9721 ccagtgctgc aatgataccg cggcttccac gctcaccggc tccagattta tcagcaataa
     9781 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9841 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9901 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9961 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    10021 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10081 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    10141 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10201 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10261 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10321 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10381 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10441 cacggaaatg ttgaatactc at
//
