LOCUS       AiP1840                 4846 bp    DNA     circular UNA 09-APR-2024
DEFINITION  pAAV-AiE2450m-SYFP2-WPRE-BGHpA.
ACCESSION   Circular
KEYWORDS    .
SOURCE       (AiP1840 - pAAV-MGT_E450-SYFP2-WPRE-BGHpA)
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             complement(1..130)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="92.20%"
                     /Primary_Match="92.20%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     Enhancer        162..846
                     /X-Transferred_From="AiE2450m"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13ya-hkqydmj"">AiE2450m</a
                     >"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:18/-330; <a
                     href=""urn:local:yoav.ben-simon:104n-hkqxixt"">AiE0808m</a
                     >"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:45/-337; <a
                     href=""urn:local:yoav.ben-simon:10z0-hkqxp2e"">AiE1031m</a
                     >"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:21/-383; <a
                     href=""urn:local:yoav.ben-simon:13yb-hkqydmj"">AiE2451m</a
                     >"
                     /X-Alternative_Match="100.00%;
                     Adjusted-Interval-0:307/-126; <a
                     href=""urn:local:yoav.ben-simon:10zy-hkqxpe8"">AiE1032m</a
                     >"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AiE2450m"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13ya-hkqydmj"">AiE2450m</a
                     >"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:18/-330;
                     <a
                     href=""urn:local:yoav.ben-simon:104n-hkqxixt"">AiE0808m</a
                     >"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:45/-337;
                     <a
                     href=""urn:local:yoav.ben-simon:10z0-hkqxp2e"">AiE1031m</a
                     >"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:21/-383;
                     <a
                     href=""urn:local:yoav.ben-simon:13yb-hkqydmj"">AiE2451m</a
                     >"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:307/-126;
                     <a
                     href=""urn:local:yoav.ben-simon:10zy-hkqxpe8"">AiE1032m</a
                     >"
                     /label="AiE2450m"
     regulatory      858..867
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="Kozak sequence"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kozak sequence"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /label="Kozak sequence"
     CDS             864..1583
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SYFP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cz-gl1nwdj"">SYFP2</a>"
                     /label="SYFP2"
     3'UTR           1618..1863
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="WPRE3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1e2-gl1nwgr"">WPRE3</a>"
                     /label="WPRE3"
     polyA_signal    1877..2080
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BGH-pA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14r-gl1nvql"">BGH-pA</a>"
                     /label="BGH-pA"
     LTR             2109..2249
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     rep_origin      2341..2647
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="f1 Origin"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="f1 Origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /label="f1 Origin"
     promoter        3061..3165
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vx7-gmqsxiz"">AmpR
                     promoter</a>"
                     /label="AmpR promoter"
     misc_feature    3096..3103
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:174-gl1nvx6"">FRT3 spacer
                     (FASEBJ 2011, 25, 1)</a>"
                     /label="FRT3 spacer (FASEBJ 2011, 25, 1)"
     CDS             3166..4023
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13p-gl1nvn3"">AmpR</a>"
                     /label="AmpR"
     rep_origin      4174..4841
                     /vntifkey=33
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC ori"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC\ori
                     (<a href=""urn:local:yoav.ben-simon:vxh-gmqt1zl"">pUC
                     ori</a>)"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaag agtaataatc gaagtgatag atggcagaga
      181 cacaaacaga cgaccgatcg gccggcctag ctctagagac tctcacctgt ctgtcctgtt
      241 ggttatccgg acggttagcc agaggatccg gggcccccgc acagctccgg gactctggaa
      301 gatagttccg agggtgggga ccttcgagaa ccagcccaca ctgaactcct ccctccttgt
      361 ggcagcagca aggtgggacg ggccaggacg tctgcttagc acctcctcca gaaatgcagc
      421 acttgggggg cccccaccct ttcgcgcgct ccttcccacc gacctcccag gggtgcacct
      481 ctccagcctc ggtcgcgctt ccgaaacctt tggtgccccc ttttcctggt cccgaccccc
      541 cacctcacgc cccctggtct ggacagcatc tccccctcgc cgccctccgc ccacgcaccg
      601 cctgactccg aggggtgcga gcgcattggg ctgcgcccgc gtgggggcgc cgcgccagcc
      661 tcgcgtagct gttctgacgc tgccgtcgcc gccgccctcc gcagcccagc cggcacccgc
      721 accagctctg cagtgcactc gtcgcctctc gggccggtcc caccaagagc cagactgtcg
      781 tgaccggggc cagcctcgaa cgtcaggcgc gagggtcatg agccagagcg ccctggggcg
      841 ccgcgccgct accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
      901 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      961 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca
     1021 agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcgtg cagtgcttcg
     1081 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1141 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1201 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1261 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     1321 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     1381 aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     1441 ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc aaagacccca
     1501 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     1561 gcatggacga gctgtacaag taagtcgacg gcgcgccgcg gccgcgaatt cgatatcata
     1621 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     1681 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     1741 tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg gcggaactca
     1801 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     1861 tggctcgaga gatcttcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     1921 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     1981 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     2041 gacagcaagg gggaggattg ggaagacaat agcaggcatg agatctcacg tgcggaccga
     2101 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     2161 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     2221 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca
     2281 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     2341 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     2401 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     2461 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     2521 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     2581 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     2641 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     2701 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     2761 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     2821 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     2881 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     2941 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     3001 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     3061 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     3121 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     3181 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     3241 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     3301 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     3361 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     3421 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     3481 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     3541 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     3601 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     3661 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     3721 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     3781 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     3841 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     3901 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     3961 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     4021 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     4081 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     4141 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4201 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4261 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4321 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     4381 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4441 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4501 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4561 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4621 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4681 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4741 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4801 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
