LOCUS       AiP1926                 4755 bp    DNA     circular UNA 09-APR-2024
DEFINITION  pAAV-AiE2554m-minBG-SYFP2-WPRE-BGHpA.
ACCESSION   Circular
KEYWORDS    .
SOURCE       (AiP1926 - pAAV-MGT_E554-minBGpromoter-SYFP2-WPRE-BGHpA)
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             complement(1..130)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="92.20%"
                     /Primary_Match="92.20%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     Enhancer        145..665
                     /X-Transferred_From="AiE2554m"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14az-hkqyftg"">AiE2554m</a
                     >"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AiE2554m"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14az-hkqyftg"">AiE2554m</a
                     >"
                     /label="AiE2554m"
     promoter        680..732
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Beta Globin minimal promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; minBG
                     promoter (<a
                     href=""urn:local:yoav.ben-simon:14q-gl1nvql"">Beta Globin
                     minimal promoter</a>)"
                     /label="minBG promoter"
     regulatory      767..776
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="Kozak sequence"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kozak sequence"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /label="Kozak sequence"
     CDS             773..1492
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SYFP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cz-gl1nwdj"">SYFP2</a>"
                     /label="SYFP2"
     3'UTR           1527..1772
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="WPRE3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1e2-gl1nwgr"">WPRE3</a>"
                     /label="WPRE3"
     polyA_signal    1786..1989
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BGH-pA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14r-gl1nvql"">BGH-pA</a>"
                     /label="BGH-pA"
     LTR             2018..2158
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     rep_origin      2250..2556
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="f1 Origin"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="f1 Origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /label="f1 Origin"
     promoter        2970..3074
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vx7-gmqsxiz"">AmpR
                     promoter</a>"
                     /label="AmpR promoter"
     misc_feature    3005..3012
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:174-gl1nvx6"">FRT3 spacer
                     (FASEBJ 2011, 25, 1)</a>"
                     /label="FRT3 spacer (FASEBJ 2011, 25, 1)"
     CDS             3075..3932
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13p-gl1nvn3"">AmpR</a>"
                     /label="AmpR"
     rep_origin      4083..4750
                     /vntifkey=33
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC ori"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC\ori
                     (<a href=""urn:local:yoav.ben-simon:vxh-gmqt1zl"">pUC
                     ori</a>)"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgaagga gttgagccct ctgactcctc ttagaatttt
      181 aaagtaagcc atatagggga ggaaaaaaaa tacttctcaa agttgacaca aatgtttagg
      241 ggaaatcaca gaatattgtt taaaggtttt agctgaaagc ccgatatttg agaattagtg
      301 aagaggttta atattgttgt gtagtttgtg tagcttgctg tttgtgggaa cttgcagata
      361 caatgcatac agactcgtcg gtgcccctcc gcaatcacac acactggctg ctggatatgg
      421 cagtgatatc acagagagtg agttagcatc tgttcagccc tgagtcagtg acatatgctg
      481 tgtcttattt tgactagcta gtagctcgag atttaagaaa aatatgatct tagaaaaata
      541 tgacaaaatt gcattttagt actccgagat ttgtgtttgt ggggctattc taaacacaaa
      601 tcctttcatt tgaaatagtg ctgaaattat gtcatcttgc ttttcctgta aatttttgga
      661 agagacgccg gcggagctcg ggctgggcat aaaagtcagg gcagagccat ctattgctta
      721 catttgcttc tgggatccag atctttcgaa gctagcgcta ccggtcgcca ccatggtgag
      781 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      841 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      901 gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      961 caccctgggc tacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     1021 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     1081 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     1141 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1201 gtacaactac aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa
     1261 ggccaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta
     1321 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1381 ctaccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     1441 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aagtcgacgg
     1501 cgcgccgcgg ccgcgaattc gatatcataa tcaacctctg gattacaaaa tttgtgaaag
     1561 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     1621 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     1681 ctggttagtt cttgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     1741 ggctcggctg ttgggcactg acaattccgt ggctcgagag atcttcgact gtgccttcta
     1801 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     1861 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     1921 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     1981 gcaggcatga gatctcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
     2041 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     2101 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
     2161 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     2221 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     2281 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     2341 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     2401 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     2461 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     2521 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
     2581 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     2641 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     2701 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     2761 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     2821 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     2881 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     2941 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3001 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3061 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     3121 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     3181 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     3241 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     3301 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     3361 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     3421 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     3481 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     3541 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     3601 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     3661 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3721 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3781 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3841 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3901 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3961 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     4021 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     4081 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     4141 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     4201 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     4261 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     4321 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4381 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4441 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4501 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4561 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4621 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4681 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4741 cttttgctca catgt
//
