LOCUS       AiP2054                 4538 bp    DNA     circular UNA 09-APR-2024
DEFINITION  pAAV-AiE2612m-minBG-SYFP2-WPRE-BGHpA.
ACCESSION   Circular
KEYWORDS    .
SOURCE       (AiP2054 - pAAV-MGT_E612-minBGpromoter-SYFP2-WPRE-BGHpA)
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             complement(1..130)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="92.20%"
                     /Primary_Match="92.20%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     Enhancer        145..448
                     /X-Transferred_From="AiE2612m"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14j7-hkqyhnq"">AiE2612m</a
                     >"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AiE2612m"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14j7-hkqyhnq"">AiE2612m</a
                     >"
                     /label="AiE2612m"
     promoter        463..515
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Beta Globin minimal promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; minBG
                     promoter (<a
                     href=""urn:local:yoav.ben-simon:14q-gl1nvql"">Beta Globin
                     minimal promoter</a>)"
                     /label="minBG promoter"
     regulatory      550..559
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="Kozak sequence"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kozak sequence"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /label="Kozak sequence"
     CDS             556..1275
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SYFP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cz-gl1nwdj"">SYFP2</a>"
                     /label="SYFP2"
     3'UTR           1310..1555
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="WPRE3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1e2-gl1nwgr"">WPRE3</a>"
                     /label="WPRE3"
     polyA_signal    1569..1772
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BGH-pA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14r-gl1nvql"">BGH-pA</a>"
                     /label="BGH-pA"
     LTR             1801..1941
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     rep_origin      2033..2339
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="f1 Origin"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="f1 Origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /label="f1 Origin"
     promoter        2753..2857
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vx7-gmqsxiz"">AmpR
                     promoter</a>"
                     /label="AmpR promoter"
     misc_feature    2788..2795
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:174-gl1nvx6"">FRT3 spacer
                     (FASEBJ 2011, 25, 1)</a>"
                     /label="FRT3 spacer (FASEBJ 2011, 25, 1)"
     CDS             2858..3715
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13p-gl1nvn3"">AmpR</a>"
                     /label="AmpR"
     rep_origin      3866..4533
                     /vntifkey=33
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC ori"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC\ori
                     (<a href=""urn:local:yoav.ben-simon:vxh-gmqt1zl"">pUC
                     ori</a>)"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtagacct ccaaaccctg aagagggtct gtcttaaagc
      181 atttgattca gagacttctg attctggtca agaagaaaac cttaccttct gattctagga
      241 tgacacatac tagctcagag gctaacacga gtgacttctc atggtgttca agccagtttt
      301 tagctgttca gtaaggtttc tgagaaagag ggattgagca aaagtcctgt aggcatcctt
      361 ttccatcctt tccagggtgg gagatgactc ctggtcctcc tcctctctcc tccttgctca
      421 tcaagcagag tgcttccttt tggcttcccg ccggcggagc tcgggctggg cataaaagtc
      481 agggcagagc catctattgc ttacatttgc ttctgggatc cagatctttc gaagctagcg
      541 ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      601 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      661 ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc
      721 gtgccctggc ccaccctcgt gaccaccctg ggctacggcg tgcagtgctt cgcccgctac
      781 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      841 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      901 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      961 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc
     1021 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc
     1081 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     1141 ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc caacgagaag
     1201 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     1261 gagctgtaca agtaagtcga cggcgcgccg cggccgcgaa ttcgatatca taatcaacct
     1321 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     1381 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     1441 attttctcct ccttgtataa atcctggtta gttcttgcca cggcggaact catcgccgcc
     1501 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggctcga
     1561 gagatcttcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     1621 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     1681 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     1741 gggggaggat tgggaagaca atagcaggca tgagatctca cgtgcggacc gagcggccgc
     1801 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     1861 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     1921 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
     1981 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     2041 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     2101 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     2161 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     2221 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     2281 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     2341 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
     2401 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     2461 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2521 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     2581 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     2641 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     2701 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     2761 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     2821 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     2881 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     2941 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     3001 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     3061 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     3121 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     3181 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     3241 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     3301 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     3361 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     3421 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     3481 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     3541 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     3601 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     3661 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     3721 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     3781 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     3841 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3901 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3961 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4021 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4081 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4141 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4201 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4261 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4321 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4381 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4441 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4501 acggttcctg gccttttgct ggccttttgc tcacatgt
//
