LOCUS       AiP1788                 4904 bp    DNA     circular UNA 09-APR-2024
DEFINITION  pAAV-AiE2116m-minBG-iCre(R297T)-BGHpA.
ACCESSION   Circular
KEYWORDS    .
SOURCE       (AiP1788 - pAAV-MGT_E116-minBGprom-iCre(R297T)-BGHpA)
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             complement(1..130)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="92.20%"
                     /Primary_Match="92.20%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     Enhancer        145..742
                     /X-Transferred_From="AiE2116m"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:12pv-hkqy3mv"">AiE2116m</a
                     >"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AiE2116m"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:12pv-hkqy3mv"">AiE2116m</a
                     >"
                     /label="AiE2116m"
     promoter        765..817
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Beta Globin minimal promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; minBG
                     promoter (<a
                     href=""urn:local:yoav.ben-simon:14q-gl1nvql"">Beta Globin
                     minimal promoter</a>)"
                     /label="minBG promoter"
     regulatory      840..849
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="Kozak sequence"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kozak sequence"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /label="Kozak sequence"
     regulatory      complement(844..853)
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="Kozak sequence"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kozak sequence"
                     /Transferred_Similarity="90.00%"
                     /Primary_Match="90.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /label="Kozak sequence"
     CDS             846..1901
                     /vntifkey=21
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="iCre(297T)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vxt-gmqtbht"">iCre(297T)</
                     a>"
                     /label="iCre(297T)"
     polyA_signal    1935..2138
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BGH-pA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14r-gl1nvql"">BGH-pA</a>"
                     /label="BGH-pA"
     LTR             2167..2307
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     rep_origin      2399..2705
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="f1 Origin"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="f1 Origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /label="f1 Origin"
     promoter        3119..3223
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vx7-gmqsxiz"">AmpR
                     promoter</a>"
                     /label="AmpR promoter"
     misc_feature    3154..3161
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:174-gl1nvx6"">FRT3 spacer
                     (FASEBJ 2011, 25, 1)</a>"
                     /label="FRT3 spacer (FASEBJ 2011, 25, 1)"
     CDS             3224..4081
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13p-gl1nvn3"">AmpR</a>"
                     /label="AmpR"
     rep_origin      4232..4899
                     /vntifkey=33
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC ori"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC\ori
                     (<a href=""urn:local:yoav.ben-simon:vxh-gmqt1zl"">pUC
                     ori</a>)"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttcacac ctggacgaca cctgtgaagg tcactgtcct
      181 tcactcacac ctggacacct gtgaaggtta ctgtcctcca ctcacacctg aatgacacct
      241 gtgaaagtca aggtcacaca aaaaggcact tttgtgctta tgcttctgtt ttaatctggg
      301 aacccagatg ttctatcagc atgctttatt ctgaggcccc cgcacatcct tcatgtatgc
      361 aagccttctc ttatgtaacc ttttggcttt ctgtggccag atggcttcct ctttcattca
      421 tccctatgtg aaccaactat gactccaaaa tatctggcct ttgtgtatca catatactcc
      481 agctttctgg tcttttttag gttccttgaa atatgccagg ctgtttcctc ttcctggggc
      541 ttcacagtag cagtctggta tacctgggat attttttctc ttatcctcat tatatgtgtt
      601 cctttctaaa tgtcacttct catggaatcc actttaccta atttgtgtag attttcagaa
      661 tttatgccca tgccaaagat ctttaccata actggatcta atcaccttta tgaaggtaag
      721 aaaagatccc aaatttattt agctatttca ctgagtttga gctcgggctg ggcataaaag
      781 tcagggcaga gccatctatt gcttacattt gcttctggga tccagatctt tccaagctag
      841 ccaccatggt gcccaagaag aagaggaaag tctccaacct gctgactgtg caccaaaacc
      901 tgcctgccct ccctgtggat gccacctctg atgaagtcag gaagaacctg atggacatgt
      961 tcagggacag gcaggccttc tctgaacaca cctggaagat gctcctgtct gtgtgcagat
     1021 cctgggctgc ctggtgcaag ctgaacaaca ggaaatggtt ccctgctgaa cctgaggatg
     1081 tgagggacta cctcctgtac ctgcaagcca gaggcctggc tgtgaagacc atccaacagc
     1141 acctgggcca gctcaacatg ctgcacagga gatctggcct gcctcgccct tctgactcca
     1201 atgctgtgtc cctggtgatg aggagaatca gaaaggagaa tgtggatgct ggggagagag
     1261 ccaagcaggc cctggccttt gaacgcactg actttgacca agtcagatcc ctgatggaga
     1321 actctgacag atgccaggac atcaggaacc tggccttcct gggcattgcc tacaacaccc
     1381 tgctgcgcat tgccgaaatt gccagaatca gagtgaagga catctcccgc accgatggtg
     1441 ggagaatgct gatccacatt ggcaggacca agaccctggt gtccacagct ggtgtggaga
     1501 aggccctgtc cctgggggtt accaagctgg tggagagatg gatctctgtg tctggtgtgg
     1561 ctgatgaccc caacaactac ctgttctgcc gggtcagaaa gaatggtgtg gctgcccctt
     1621 ctgccacctc ccaactgtcc acccgggccc tggaagggat ctttgaggcc acccaccgcc
     1681 tgatctatgg tgccaaggat gactctgggc agagatacct ggcctggtct ggccactctg
     1741 ccagagtggg tgctgccacc gacatggcca gggctggtgt gtccatccct gaaatcatgc
     1801 aggctggtgg ctggaccaat gtgaacattg tgatgaacta catcagaaac ctggactctg
     1861 agactggggc catggtgagg ctgctcgaag atggggactg aggcgcgccg aattcaagct
     1921 tctcgagaga tcttcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     1981 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     2041 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     2101 cagcaagggg gaggattggg aagacaatag caggcatgag atctcacgtg cggaccgagc
     2161 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     2221 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     2281 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     2341 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     2401 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     2461 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     2521 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     2581 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     2641 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     2701 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
     2761 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     2821 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     2881 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     2941 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     3001 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     3061 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     3121 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3181 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3241 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3301 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3361 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     3421 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     3481 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3541 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3601 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     3661 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3721 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3781 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     3841 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3901 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     3961 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4021 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4081 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4141 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4201 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4261 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4321 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4381 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     4441 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4501 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4561 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4621 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4681 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4741 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4801 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4861 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
